pycom03g01450

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000940
Physical Location & Seq
Reverse (-)
1085016 .. 1087069
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g01450.4

Sequence Viewer

Length: 1170 bp
ATGCCCAAGTCAGCGAAGCGCGAACGGAGCGCGAGTCAAACCCGAGGCGTTGACGAGGCCGCGCAGCTCAGAGCCGTGAAGCGTGAGAAGGTCAACGCCGGCGACCGAGTCGATGACGAGTCGGCGATCCCGAGTGAGCAGGAAGCAGTCAGGCGGCGGGCACTGCGGTCCGACTACCTCGCCGTCAAGAACCTCATCAGTGAGAAAAGAGATGATTTGATGTGTTCGGATTTGGAGAAGTTCGGACTTCTCATCAATAAAGTCGAGAAATTGCATGAGCAAGTTCAGAAGCCAAGGGAACAAGTAGCAGACGCAGAGGCACTGCTGGACATAACAAACACATTGTGGACCTCTGTTAAGTCACAGTCCAGTGCCGGGATTAGCCCGTCGGACTTTGTTACCGCTTTGCTTAGCAATTTTAGCCAACCGAGAGGAGGGGATGACCACGTCACGGTTAATTGGAAGGGAATTGGGTTTTTTGTTTTGCCCATTTTCAAGAGCGCTCATGGTTGTTGCACTATGCTTGGACCCATGAGTACCGAGTTGAAGCAAAGGAAGGCTGTGGTTCGCAGTAAACGTGTGAAGCCTACCACAACTGATCGGCCTGATGAGATAGATGATACTCAGATGGAAGAGAAAACTGATACTGACAAGAACATGTCAACGATATTTGACATTCTAAGAAGGAAAAAGCGGAGTCGACTTGAGGCTTTGATATTAAACAGAAATTCTTTTGCGCAGACGGTGGAGAACTTATTTGCACTGTCATTCTTAGTCAAAGATGGCCGAGCTGAATTATCTGTAGATGCAAATGATTTTCATATTGTTTCACCTAGGAATGCTCCTTCCGCCGAGGACGTGGCATCACGGAAGGTCACTTACCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGGTAATGATGGATAGTTTGCCTGTTGGTGAGGAGCTGATGCCTCATAGATTTCCCCCAAACTCTACCCGTACCTCTCAAGAGGAACCAGCATCATCTGGTTCCCAAACAGCCTTACCCCCAACCCCGATTCGAAAACTCTCTAGAAACCGTGGCCGGGTTGTGCAGGAAGAATCTATTGCAGAAGAATCCCCTGAAAACGATGAAGCTACTGGAACTAACGTTTTTCGCAGGTCTAAGCGCAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

390

Amino Acids

43.89

Weight (kDa)

8.97

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 1140
AccI GTMKAC 1 cut(s) 700
AccII CGCG 3 cut(s) 21, 32, 62
AciI CCGC 7 cut(s) 60, 154, 157, 166, 402, 694, 849
AclI AACGTT 1 cut(s) 1140
AclWI GGATC 1 cut(s) 121
AcoI YGGCCR 2 cut(s) 784, 1072
AcsI RAATTY 1 cut(s) 727
AdeI CACNNNGTG 1 cut(s) 345
AfaI GTAC 2 cut(s) 538, 991
AfeI AGCGCT 1 cut(s) 502
AfiI CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1075
AflIII ACRYGT 2 cut(s) 577, 657
AgsI TTSAA 3 cut(s) 496, 547, 910
AjiI CACGTC 2 cut(s) 448, 859
AluBI AGCT 5 cut(s) 67, 791, 955, 1127, 1165
AluI AGCT 5 cut(s) 67, 791, 955, 1127, 1165
AlwI GGATC 1 cut(s) 121
Ama87I CYCGRG 2 cut(s) 42, 130
Aor51HI AGCGCT 1 cut(s) 502
AoxI GGCC 4 cut(s) 57, 602, 784, 1072
ApeKI GCWGC 1 cut(s) 64
ApoI RAATTY 1 cut(s) 727
AspA2I CCTAGG 1 cut(s) 833
AspLEI GCGC 6 cut(s) 21, 32, 64, 503, 739, 1161
AspS9I GGNCC 3 cut(s) 168, 348, 527
AsuC2I CCSGG 2 cut(s) 376, 1076
AsuHPI GGTGA 2 cut(s) 822, 959
AsuII TTCGAA 1 cut(s) 1051
AvaI CYCGRG 2 cut(s) 42, 130
AvaII GGWCC 3 cut(s) 168, 348, 527
AvrII CCTAGG 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 863, 895
BbvI GCAGC 1 cut(s) 76
BccI CCATC 3 cut(s) 622, 776, 922
BceAI ACGGC 2 cut(s) 59, 167
BcnI CCSGG 2 cut(s) 376, 1076
BfaI CTAG 2 cut(s) 834, 1062
BfmI CTRYAG 1 cut(s) 801
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 1 cut(s) 1140
BisI GCNGC 3 cut(s) 60, 65, 155
BlnI CCTAGG 1 cut(s) 833
BlpI GCTNAGC 1 cut(s) 410
BlsI GCNGC 3 cut(s) 61, 66, 156
Bme1390I CCNGG 2 cut(s) 376, 1076
Bme18I GGWCC 3 cut(s) 168, 348, 527
BmeT110I CYCGRG 2 cut(s) 42, 130
BmgBI CACGTC 2 cut(s) 448, 859
BmgT120I GGNCC 3 cut(s) 168, 348, 527
BmiI GGNNCC 3 cut(s) 529, 1005, 1021
BmrFI CCNGG 2 cut(s) 376, 1076
BmsI GCATC 4 cut(s) 796, 872, 948, 1019
Bpu1102I GCTNAGC 1 cut(s) 410
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 2 cut(s) 725, 981
BpuMI CCSGG 2 cut(s) 376, 1076
BsaJI CCNNGG 5 cut(s) 43, 293, 833, 852, 1069
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1075
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 3 cut(s) 369, 920, 1135
BseDI CCNNGG 5 cut(s) 43, 293, 833, 852, 1069
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1075
BseMII CTCAG 2 cut(s) 82, 638
BseNI ACTGG 3 cut(s) 369, 920, 1135
BseRI GAGGAG 2 cut(s) 447, 965
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 1103
Bsh1236I CGCG 3 cut(s) 21, 32, 62
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 59, 604, 786, 1074
BsiEI CGRYCG 1 cut(s) 106
BsiHKCI CYCGRG 2 cut(s) 42, 130
BsiSI CCGG 3 cut(s) 99, 375, 1075
BslI CCNNNNNNNGG 4 cut(s) 375, 434, 451, 1075
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 4 cut(s) 59, 604, 786, 1074
BsoBI CYCGRG 2 cut(s) 42, 130
Bsp119I TTCGAA 1 cut(s) 1051
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 126, 598
Bsp1720I GCTNAGC 1 cut(s) 410
BspACI CCGC 7 cut(s) 60, 154, 157, 166, 402, 694, 849
BspANI GGCC 4 cut(s) 59, 604, 786, 1074
BspCNI CTCAG 2 cut(s) 81, 637
BspFNI CGCG 3 cut(s) 21, 32, 62
BspLI GGNNCC 3 cut(s) 529, 1005, 1021
BspMI ACCTGC 1 cut(s) 1140
BspPI GGATC 1 cut(s) 121
BspT104I TTCGAA 1 cut(s) 1051
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 3 cut(s) 369, 920, 1135
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 5 cut(s) 43, 293, 833, 852, 1069
BssMI GATC 2 cut(s) 126, 598
BssT1I CCWWGG 2 cut(s) 293, 833
Bst4CI ACNGT 5 cut(s) 366, 454, 745, 765, 1070
Bst6I CTCTTC 1 cut(s) 627
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 3 cut(s) 100, 159, 1163
BstDEI CTNAG 6 cut(s) 68, 410, 624, 680, 772, 1155
BstDSI CCRYGG 1 cut(s) 1069
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 3 cut(s) 21, 32, 62
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 6 cut(s) 21, 32, 64, 503, 739, 1161
BstKTI GATC 2 cut(s) 129, 601
BstMBI GATC 2 cut(s) 126, 598
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 4 cut(s) 27, 163, 420, 848
BstNSI RCATGY 1 cut(s) 661
BstSCI CCNGG 2 cut(s) 374, 1074
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 3 cut(s) 21, 32, 62
BstV1I GCAGC 1 cut(s) 76
BsuRI GGCC 4 cut(s) 59, 604, 786, 1074
BtgI CCRYGG 1 cut(s) 1069
BtrI CACGTC 2 cut(s) 448, 859
BtsCI GGATG 1 cut(s) 445
BtsI GCAGTG 2 cut(s) 161, 320
BtsIMutI CAGTG 5 cut(s) 161, 205, 320, 376, 761
BveI ACCTGC 1 cut(s) 1140
Cac8I GCNNGC 3 cut(s) 100, 159, 1163
CfoI GCGC 6 cut(s) 21, 32, 64, 503, 739, 1161
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 168, 348, 527
CpoI CGGWCCG 1 cut(s) 168
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 537, 990
CspI CGGWCCG 1 cut(s) 168
CviAII CATG 4 cut(s) 275, 506, 532, 658
CviQI GTAC 2 cut(s) 537, 990
DdeI CTNAG 6 cut(s) 68, 410, 624, 680, 772, 1155
DpnI GATC 2 cut(s) 128, 600
DpnII GATC 2 cut(s) 126, 598
DraIII CACNNNGTG 1 cut(s) 345
EaeI YGGCCR 2 cut(s) 784, 1072
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EciI GGCGGA 1 cut(s) 838
Eco130I CCWWGG 2 cut(s) 293, 833
Eco47I GGWCC 3 cut(s) 168, 348, 527
Eco47III AGCGCT 1 cut(s) 502
Eco88I CYCGRG 2 cut(s) 42, 130
EcoT14I CCWWGG 2 cut(s) 293, 833
ErhI CCWWGG 2 cut(s) 293, 833
FaeI CATG 4 cut(s) 278, 509, 535, 661
FaiI YATR 8 cut(s) 276, 332, 507, 521, 533, 659, 822, 966
FatI CATG 4 cut(s) 274, 505, 531, 657
FauI CCCGC 1 cut(s) 150
FblI GTMKAC 1 cut(s) 700
Fnu4HI GCNGC 3 cut(s) 60, 65, 155
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 3 cut(s) 60, 65, 155
FspBI CTAG 2 cut(s) 834, 1062
FspI TGCGCA 1 cut(s) 738
GlaI GCGC 6 cut(s) 20, 31, 63, 502, 738, 1160
GluI GCNGC 3 cut(s) 60, 65, 155
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 4 cut(s) 59, 604, 786, 1074
HapII CCGG 3 cut(s) 99, 375, 1075
HgaI GACGC 1 cut(s) 320
HhaI GCGC 6 cut(s) 21, 32, 64, 503, 739, 1161
Hin1II CATG 4 cut(s) 278, 509, 535, 661
Hin6I GCGC 6 cut(s) 19, 30, 62, 501, 737, 1159
HinP1I GCGC 6 cut(s) 19, 30, 62, 501, 737, 1159
HincII GTYRAC 4 cut(s) 52, 94, 663, 701
HindII GTYRAC 4 cut(s) 52, 94, 663, 701
HindIII AAGCTT 1 cut(s) 1163
HinfI GANTC 7 cut(s) 34, 108, 119, 697, 1048, 1091, 1106
HpaII CCGG 3 cut(s) 99, 375, 1075
HphI GGTGA 2 cut(s) 822, 959
Hpy166II GTNNAC 6 cut(s) 52, 94, 348, 575, 663, 701
Hpy188I TCNGA 8 cut(s) 71, 172, 229, 245, 288, 391, 627, 899
Hpy188III TCNNGA 6 cut(s) 130, 187, 265, 496, 998, 1062
Hpy8I GTNNAC 6 cut(s) 52, 94, 348, 575, 663, 701
Hpy99I CGWCG 1 cut(s) 391
HpyAV CCTTC 7 cut(s) 82, 457, 550, 678, 855, 865, 913
HpyCH4III ACNGT 5 cut(s) 366, 454, 745, 765, 1070
HpyCH4IV ACGT 4 cut(s) 447, 577, 858, 1140
HpyCH4V TGCA 6 cut(s) 274, 516, 761, 809, 1084, 1100
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 163, 420, 848
HpyF3I CTNAG 6 cut(s) 68, 410, 624, 680, 772, 1155
HpySE526I ACGT 4 cut(s) 447, 577, 858, 1140
Hsp92II CATG 4 cut(s) 278, 509, 535, 661
HspAI GCGC 6 cut(s) 19, 30, 62, 501, 737, 1159
KroI GCCGGC 1 cut(s) 98
KroNI GCCGGC 1 cut(s) 100
Kzo9I GATC 2 cut(s) 126, 598
LmnI GCTCC 3 cut(s) 27, 847, 952
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 4 cut(s) 796, 872, 948, 1019
MaeI CTAG 2 cut(s) 834, 1062
MaeII ACGT 4 cut(s) 447, 577, 858, 1140
MaeIII GTNAC 4 cut(s) 360, 397, 448, 874
MalI GATC 2 cut(s) 128, 600
MboI GATC 2 cut(s) 126, 598
MboII GAAGA 3 cut(s) 644, 1100, 1115
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 6 cut(s) 269, 415, 457, 468, 727, 794
MlyI GAGTC 4 cut(s) 43, 117, 128, 706
MmeI TCCRAC 2 cut(s) 195, 369
MreI CGCCGGCG 1 cut(s) 98
MroNI GCCGGC 1 cut(s) 98
MseI TTAA 4 cut(s) 357, 456, 719, 1168
MspI CCGG 3 cut(s) 99, 375, 1075
MspR9I CCNGG 2 cut(s) 376, 1076
Mva1269I GAATGC 1 cut(s) 844
MvnI CGCG 3 cut(s) 21, 32, 62
MwoI GCNNNNNNNGC 4 cut(s) 27, 163, 420, 848
NaeI GCCGGC 1 cut(s) 100
NciI CCSGG 2 cut(s) 376, 1076
NdeII GATC 2 cut(s) 126, 598
NgoMIV GCCGGC 1 cut(s) 98
NlaIII CATG 4 cut(s) 278, 509, 535, 661
NlaIV GGNNCC 3 cut(s) 529, 1005, 1021
NmeAIII GCCGAG 2 cut(s) 812, 877
NmuCI GTSAC 3 cut(s) 360, 448, 874
NsbI TGCGCA 1 cut(s) 738
NspI RCATGY 1 cut(s) 661
NspV TTCGAA 1 cut(s) 1051
PciI ACATGT 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 574
PctI GAATGC 1 cut(s) 844
PdiI GCCGGC 1 cut(s) 100
PfeI GAWTC 3 cut(s) 1048, 1091, 1106
PflFI GACNNNGTC 2 cut(s) 107, 446
PkrI GCNGC 3 cut(s) 61, 66, 156
PleI GAGTC 4 cut(s) 42, 116, 127, 705
PpsI GAGTC 4 cut(s) 42, 116, 127, 705
PscI ACATGT 1 cut(s) 657
Psp1406I AACGTT 1 cut(s) 1140
PspN4I GGNNCC 3 cut(s) 529, 1005, 1021
PspPI GGNCC 3 cut(s) 168, 348, 527
PsyI GACNNNGTC 2 cut(s) 107, 446
RsaI GTAC 2 cut(s) 538, 991
RsaNI GTAC 2 cut(s) 537, 990
Rsr2I CGGWCCG 1 cut(s) 168
RsrII CGGWCCG 1 cut(s) 168
SalI GTCGAC 1 cut(s) 699
SaqAI TTAA 4 cut(s) 357, 456, 719, 1168
SatI GCNGC 3 cut(s) 60, 65, 155
Sau3AI GATC 2 cut(s) 126, 598
Sau96I GGNCC 3 cut(s) 168, 348, 527
SchI GAGTC 4 cut(s) 43, 117, 128, 706
ScrFI CCNGG 2 cut(s) 376, 1076
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 4 cut(s) 796, 872, 948, 1019
SfcI CTRYAG 1 cut(s) 801
SfuI TTCGAA 1 cut(s) 1051
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 3 cut(s) 168, 348, 527
SmlI CTYRAG 2 cut(s) 704, 996
SmoI CTYRAG 2 cut(s) 704, 996
Sse9I AATT 6 cut(s) 269, 415, 457, 468, 727, 794
SsiI CCGC 7 cut(s) 60, 154, 157, 166, 402, 694, 849
SspMI CTAG 2 cut(s) 834, 1062
StyD4I CCNGG 2 cut(s) 374, 1074
StyI CCWWGG 2 cut(s) 293, 833
TaaI ACNGT 5 cut(s) 366, 454, 745, 765, 1070
TaiI ACGT 4 cut(s) 450, 580, 861, 1143
TaqI TCGA 4 cut(s) 111, 264, 700, 1051
TaqII GACCGA 1 cut(s) 120
TasI AATT 6 cut(s) 269, 415, 457, 468, 727, 794
TauI GCSGC 2 cut(s) 62, 157
TfiI GAWTC 3 cut(s) 1048, 1091, 1106
Tru1I TTAA 4 cut(s) 357, 456, 719, 1168
Tru9I TTAA 4 cut(s) 357, 456, 719, 1168
TscAI CASTG 5 cut(s) 168, 205, 327, 376, 768
TseFI GTSAC 3 cut(s) 360, 448, 874
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 3 cut(s) 360, 448, 874
TspDTI ATGAA 2 cut(s) 809, 1137
TspGWI ACGGA 2 cut(s) 40, 883
TspRI CASTG 5 cut(s) 168, 205, 327, 376, 768
Tth111I GACNNNGTC 2 cut(s) 107, 446
VpaK11BI GGWCC 3 cut(s) 168, 348, 527
XapI RAATTY 1 cut(s) 727
XbaI TCTAGA 1 cut(s) 1061
XceI RCATGY 1 cut(s) 661
XmaJI CCTAGG 1 cut(s) 833
XmiI GTMKAC 1 cut(s) 700
XspI CTAG 2 cut(s) 834, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.