MD12G1059300.v1.1

Non-structural maintenance of chromosomes element 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000940
Physical Location & Seq
Forward (+)
6920698 .. 6923237
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1059300.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGTGAAGCGTGAATTCGTCAGTAGCGGCAGCGGTGGAAGAGATGAAACTGAACGACCGCAAGCGGTGGAGGACCGGAGAGCTCTCCGATCACGCTATTTTAATGTGAAGAGCATTATCCACGATAAAAGAGAGGACATTACGAAAGTGGATTCAGACAAGTTCAATTTGATCATCAATGAAGTGGAGAGCTTGCATGAGCTTGTTCAAAATCCGAGAGAACAAGTTGCTGACGCAGAAGCGCTTTTGGACATTACAACCCACTTGATAAGCTCCGTCAAGGCACATAACAAGGAAGGAATCACAACTACAGGTTTCGTCAACTGTATTCTCAAACAGTTTGAGAACCGAAGTCGAAGTGGAAGCAGCTCAGTCTCTTGGAAGGATATTGGAAGGGCAGTCTCACATGTTTACCCGAAACCTCCCCAATGCTGCACCATGATTGGGCCTATGAGTCTTGAAGTAAAGCAACGGAAGAGTTATGTTCGTGCAAAACGCGTGAAGCCTACAGAAAATGTTACCCCTGAAGAGCTTGATGATGCCGTTGAAGAAGAGCAACAGGAGACCGTTAAAAATGTGTCAGCTATGTTCAATATCTTGAGGAAGAAGAGAAGAGTCAGGTTTGAAAATCTAGTCTTGAATGAGAATTCCTTTGCGCAGACGGTGGAAAATATATTTGCCCTCTCGTTTCTAGTTAAAGATGGGCGAGCGGAACTAAAATTGAATGAGGAAGGCCATCACCTAGTTTTGCCAAGGAATGCTCCTTCTGCCGAAAAAATTGCTTCTGGGGAGGTTACTTACGGACACTTTGTCTTCCGATTTGATTTCCAGGACTGGAAGTTCATGAAAGAGATTGTTGGGGACGGCGAGCAGCTGATGCCACACAGGACCTATGATCTATACAAACCAAGCGATCATCAAGGCGATTTGCAATGCGAAGAATCTCAAACAACGCCATCCACAATGCCAATTAGGAAGCTCTCCAGAAACAGAGGCTTGGTTATGCAGGAGCAGCCAGTTGGAGAAGGCATGGCTGAGCCTGATACTAATCAATCGATTGTGGACGACTCGCCCGTGCACGATTACGCTCAAGAAAGCGCCAATGCCATCCGAAAAGGGAAGCGTAAAATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

379

Amino Acids

43.11

Weight (kDa)

6.45

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 206 - 292 7.2e-23 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 657
AccBSI CCGCTC 1 cut(s) 710
AccII CGCG 1 cut(s) 498
AciI CCGC 5 cut(s) 27, 33, 59, 65, 710
AcsI RAATTY 2 cut(s) 14, 646
AcuI CTGAAG 1 cut(s) 546
AfeI AGCGCT 1 cut(s) 243
AfiI CCNNNNNNNGG 1 cut(s) 444
AflIII ACRYGT 2 cut(s) 406, 496
AgsI TTSAA 8 cut(s) 166, 209, 461, 548, 592, 626, 640, 724
AhdI GACNNNNNGTC 1 cut(s) 809
AjnI CCWGG 1 cut(s) 828
AloI GAACNNNNNNTCC 2 cut(s) 824, 856
AluBI AGCT 9 cut(s) 83, 192, 202, 273, 369, 532, 584, 874, 979
AluI AGCT 9 cut(s) 83, 192, 202, 273, 369, 532, 584, 874, 979
Alw21I GWGCWC 2 cut(s) 85, 1080
Alw26I GTCTC 3 cut(s) 379, 406, 557
Alw44I GTGCAC 1 cut(s) 1076
Aor51HI AGCGCT 1 cut(s) 243
AoxI GGCC 2 cut(s) 446, 733
ApaLI GTGCAC 1 cut(s) 1076
ApeKI GCWGC 5 cut(s) 30, 366, 432, 871, 1012
ApoI RAATTY 2 cut(s) 14, 646
AspLEI GCGC 3 cut(s) 244, 658, 1100
AspS9I GGNCC 3 cut(s) 73, 446, 888
AsuHPI GGTGA 2 cut(s) 16, 731
AvaII GGWCC 2 cut(s) 73, 888
BaeGI GKGCMC 1 cut(s) 1080
BanII GRGCYC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 805
Bbv12I GWGCWC 2 cut(s) 85, 1080
BbvI GCAGC 5 cut(s) 42, 378, 419, 883, 1024
BccI CCATC 4 cut(s) 695, 744, 964, 1115
BceAI ACGGC 2 cut(s) 527, 880
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 1 cut(s) 830
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 3 cut(s) 379, 406, 557
BfaI CTAG 3 cut(s) 632, 692, 743
BfmI CTRYAG 2 cut(s) 309, 507
BfoI RGCGCY 2 cut(s) 245, 1101
BisI GCNGC 6 cut(s) 28, 31, 367, 433, 872, 1013
BlpI GCTNAGC 1 cut(s) 1035
BlsI GCNGC 6 cut(s) 29, 32, 368, 434, 873, 1014
Bme1390I CCNGG 1 cut(s) 830
Bme18I GGWCC 2 cut(s) 73, 888
BmeRI GACNNNNNGTC 1 cut(s) 809
BmgT120I GGNCC 3 cut(s) 73, 446, 888
BmrFI CCNGG 1 cut(s) 830
BmsI GCATC 2 cut(s) 529, 867
BpiI GAAGAC 1 cut(s) 805
BpmI CTGGAG 1 cut(s) 967
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 2 cut(s) 619, 1074
Bsa29I ATCGAT 1 cut(s) 1055
BsaBI GATNNNNATC 1 cut(s) 1047
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 1 cut(s) 752
BsaWI WCCGGW 1 cut(s) 75
Bsc4I CCNNNNNNNGG 1 cut(s) 444
Bse1I ACTGG 2 cut(s) 839, 1016
Bse3DI GCAATG 1 cut(s) 938
Bse8I GATNNNNATC 1 cut(s) 1047
BseBI CCWGG 1 cut(s) 830
BseCI ATCGAT 1 cut(s) 1055
BseDI CCNNGG 1 cut(s) 752
BseGI GGATG 2 cut(s) 956, 1107
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 1 cut(s) 444
BseMI GCAATG 1 cut(s) 938
BseMII CTCAG 2 cut(s) 384, 1026
BseNI ACTGG 2 cut(s) 839, 1016
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 5 cut(s) 42, 378, 419, 883, 1024
BsgI GTGCAG 1 cut(s) 418
Bsh1236I CGCG 1 cut(s) 498
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 448, 735
BshVI ATCGAT 1 cut(s) 1055
BsiEI CGRYCG 1 cut(s) 59
BsiHKAI GWGCWC 2 cut(s) 85, 1080
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 1 cut(s) 444
BsmAI GTCTC 3 cut(s) 379, 406, 557
BsmFI GGGAC 1 cut(s) 875
BsmI GAATGC 1 cut(s) 763
BsnI GGCC 2 cut(s) 448, 735
Bso31I GGTCTC 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 85, 1080
Bsp143I GATC 4 cut(s) 89, 171, 895, 913
Bsp1720I GCTNAGC 1 cut(s) 1035
BspACI CCGC 5 cut(s) 27, 33, 59, 65, 710
BspANI GGCC 2 cut(s) 448, 735
BspCNI CTCAG 2 cut(s) 383, 1027
BspDI ATCGAT 1 cut(s) 1055
BspFNI CGCG 1 cut(s) 498
BspHI TCATGA 1 cut(s) 843
BspQI GCTCTTC 3 cut(s) 104, 522, 546
BspTNI GGTCTC 1 cut(s) 557
BsrBI CCGCTC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 938
BsrI ACTGG 2 cut(s) 839, 1016
BssECI CCNNGG 1 cut(s) 752
BssMI GATC 4 cut(s) 89, 171, 895, 913
BssT1I CCWWGG 1 cut(s) 752
Bst2UI CCWGG 1 cut(s) 830
Bst4CI ACNGT 4 cut(s) 326, 339, 568, 664
Bst6I CTCTTC 7 cut(s) 34, 104, 470, 522, 546, 602, 607
BstAPI GCANNNNNTGC 1 cut(s) 877
BstC8I GCNNGC 4 cut(s) 63, 194, 708, 869
BstDEI CTNAG 2 cut(s) 370, 1035
BstF5I GGATG 2 cut(s) 956, 1107
BstFNI CGCG 1 cut(s) 498
BstH2I RGCGCY 2 cut(s) 245, 1101
BstHHI GCGC 3 cut(s) 244, 658, 1100
BstKTI GATC 4 cut(s) 92, 174, 898, 916
BstMAI GTCTC 3 cut(s) 379, 406, 557
BstMBI GATC 4 cut(s) 89, 171, 895, 913
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 3 cut(s) 767, 877, 1012
BstNI CCWGG 1 cut(s) 830
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 828
BstSFI CTRYAG 2 cut(s) 309, 507
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 5 cut(s) 42, 378, 419, 883, 1024
BstV2I GAAGAC 1 cut(s) 805
Bsu15I ATCGAT 1 cut(s) 1055
BsuRI GGCC 2 cut(s) 448, 735
BsuTUI ATCGAT 1 cut(s) 1055
BtsCI GGATG 2 cut(s) 956, 1107
Cac8I GCNNGC 4 cut(s) 63, 194, 708, 869
CciI TCATGA 1 cut(s) 843
CfoI GCGC 3 cut(s) 244, 658, 1100
Cfr13I GGNCC 3 cut(s) 73, 446, 888
ClaI ATCGAT 1 cut(s) 1055
CseI GACGC 1 cut(s) 242
CviAII CATG 5 cut(s) 197, 407, 439, 844, 1030
DdeI CTNAG 2 cut(s) 370, 1035
DpnI GATC 4 cut(s) 91, 173, 897, 915
DpnII GATC 4 cut(s) 89, 171, 895, 913
DriI GACNNNNNGTC 1 cut(s) 809
Eam1104I CTCTTC 7 cut(s) 34, 104, 470, 522, 546, 602, 607
Eam1105I GACNNNNNGTC 1 cut(s) 809
EarI CTCTTC 7 cut(s) 34, 104, 470, 522, 546, 602, 607
Ecl136II GAGCTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 752
Eco24I GRGCYC 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 73, 888
Eco47III AGCGCT 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 546
EcoICRI GAGCTC 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 888
EcoRI GAATTC 2 cut(s) 14, 646
EcoRII CCWGG 1 cut(s) 828
EcoT14I CCWWGG 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 752
FaeI CATG 5 cut(s) 200, 410, 442, 847, 1033
FaqI GGGAC 1 cut(s) 875
FatI CATG 5 cut(s) 196, 406, 438, 843, 1029
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 6 cut(s) 28, 31, 367, 433, 872, 1013
FokI GGATG 2 cut(s) 943, 1094
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 6 cut(s) 28, 31, 367, 433, 872, 1013
FspBI CTAG 3 cut(s) 632, 692, 743
FspI TGCGCA 1 cut(s) 657
GlaI GCGC 3 cut(s) 243, 657, 1099
GluI GCNGC 6 cut(s) 28, 31, 367, 433, 872, 1013
GsuI CTGGAG 1 cut(s) 967
HaeII RGCGCY 2 cut(s) 245, 1101
HaeIII GGCC 2 cut(s) 448, 735
HapII CCGG 1 cut(s) 76
HgaI GACGC 1 cut(s) 242
HhaI GCGC 3 cut(s) 244, 658, 1100
Hin1II CATG 5 cut(s) 200, 410, 442, 847, 1033
Hin6I GCGC 3 cut(s) 242, 656, 1098
HinP1I GCGC 3 cut(s) 242, 656, 1098
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 6 cut(s) 152, 300, 454, 615, 941, 1067
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 16, 731
Hpy166II GTNNAC 4 cut(s) 322, 412, 1063, 1078
Hpy188I TCNGA 5 cut(s) 89, 157, 216, 818, 1112
Hpy188III TCNNGA 6 cut(s) 458, 598, 637, 844, 984, 1091
Hpy8I GTNNAC 4 cut(s) 322, 412, 1063, 1078
HpyAV CCTTC 6 cut(s) 290, 376, 387, 725, 774, 1019
HpyCH4III ACNGT 4 cut(s) 326, 339, 568, 664
HpyCH4V TGCA 6 cut(s) 196, 435, 491, 931, 1006, 1078
HpyF10VI GCNNNNNNNGC 3 cut(s) 767, 877, 1012
HpyF3I CTNAG 2 cut(s) 370, 1035
Hsp92II CATG 5 cut(s) 200, 410, 442, 847, 1033
HspAI GCGC 3 cut(s) 242, 656, 1098
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 4 cut(s) 89, 171, 895, 913
LguI GCTCTTC 3 cut(s) 104, 522, 546
LmnI GCTCC 3 cut(s) 278, 766, 1009
Lsp1109I GCAGC 5 cut(s) 42, 378, 419, 883, 1024
LweI GCATC 2 cut(s) 529, 867
MaeI CTAG 3 cut(s) 632, 692, 743
MaeIII GTNAC 2 cut(s) 517, 793
MalI GATC 4 cut(s) 91, 173, 897, 915
MbiI CCGCTC 1 cut(s) 710
MboI GATC 4 cut(s) 89, 171, 895, 913
MhlI GDGCHC 2 cut(s) 85, 1080
MluCI AATT 6 cut(s) 14, 166, 646, 719, 777, 969
MluI ACGCGT 1 cut(s) 496
MlyI GAGTC 3 cut(s) 463, 624, 1061
MmeI TCCRAC 1 cut(s) 1000
MnlI CCTC 8 cut(s) 64, 127, 432, 594, 692, 721, 784, 986
MseI TTAA 3 cut(s) 102, 570, 696
MspA1I CMGCKG 2 cut(s) 33, 874
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 763
MvaI CCWGG 1 cut(s) 830
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 3 cut(s) 767, 877, 1012
NdeII GATC 4 cut(s) 89, 171, 895, 913
NlaIII CATG 5 cut(s) 200, 410, 442, 847, 1033
NsbI TGCGCA 1 cut(s) 657
NspI RCATGY 1 cut(s) 410
PagI TCATGA 1 cut(s) 843
PciI ACATGT 1 cut(s) 406
PciSI GCTCTTC 3 cut(s) 104, 522, 546
PcsI WCGNNNNNNNCGW 1 cut(s) 1071
PctI GAATGC 1 cut(s) 763
PfeI GAWTC 3 cut(s) 152, 300, 941
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 6 cut(s) 29, 32, 368, 434, 873, 1014
PleI GAGTC 3 cut(s) 462, 623, 1061
PpsI GAGTC 3 cut(s) 462, 623, 1061
PpuMI RGGWCCY 1 cut(s) 888
PscI ACATGT 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 85
Psp5II RGGWCCY 1 cut(s) 888
Psp6I CCWGG 1 cut(s) 828
PspGI CCWGG 1 cut(s) 828
PspPI GGNCC 3 cut(s) 73, 446, 888
PspPPI RGGWCCY 1 cut(s) 888
PvuII CAGCTG 1 cut(s) 874
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 3 cut(s) 104, 522, 546
SaqAI TTAA 3 cut(s) 102, 570, 696
SatI GCNGC 6 cut(s) 28, 31, 367, 433, 872, 1013
Sau3AI GATC 4 cut(s) 89, 171, 895, 913
Sau96I GGNCC 3 cut(s) 73, 446, 888
SchI GAGTC 3 cut(s) 463, 624, 1061
ScrFI CCNGG 1 cut(s) 830
SduI GDGCHC 2 cut(s) 85, 1080
SfaNI GCATC 2 cut(s) 529, 867
SfcI CTRYAG 2 cut(s) 309, 507
SinI GGWCC 2 cut(s) 73, 888
SmlI CTYRAG 2 cut(s) 598, 1089
SmoI CTYRAG 2 cut(s) 598, 1089
Sse9I AATT 6 cut(s) 14, 166, 646, 719, 777, 969
SsiI CCGC 5 cut(s) 27, 33, 59, 65, 710
SspMI CTAG 3 cut(s) 632, 692, 743
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 828
StyI CCWWGG 1 cut(s) 752
TaaI ACNGT 4 cut(s) 326, 339, 568, 664
TaqI TCGA 2 cut(s) 355, 1055
TasI AATT 6 cut(s) 14, 166, 646, 719, 777, 969
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 3 cut(s) 152, 300, 941
Tru1I TTAA 3 cut(s) 102, 570, 696
Tru9I TTAA 3 cut(s) 102, 570, 696
TseI GCWGC 5 cut(s) 30, 366, 432, 871, 1012
TspDTI ATGAA 4 cut(s) 60, 195, 832, 860
TspGWI ACGGA 3 cut(s) 265, 487, 816
VneI GTGCAC 1 cut(s) 1076
VpaK11BI GGWCC 2 cut(s) 73, 888
XapI RAATTY 2 cut(s) 14, 646
XceI RCATGY 1 cut(s) 410
XspI CTAG 3 cut(s) 632, 692, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.