Rh5AG522900

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000940
Physical Location & Seq
Forward (+)
89759107 .. 89760499
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG522900.1

Sequence Viewer

Length: 642 bp
ATGGCTGAGAAAGAGTGTACTGTCAATTTTGAGCTACTCAACTTCGGCCAAGACCCGGTGCCACGGCGAGAGCTCCGCTCCGACTACCTCGCCGTCCGGAGCTTCACCAGTGAGAAAAGAGACGATTTAATGAGTCCGGATTCGATGAAGTTTGATGTGATCATTGATATAGTTGACAAACTGCATGAGCAAGTTCAGCTTCCGAGAGAAAAAGTAGCGGATGCACAAGCACTTGTCCAAGTAACACCGTGGTGGCCTCTGCTTGGACCCATGAATATTCAGATTAAGCAATGGAAGGCTGCTGTCCGGCGTACAAAGCCTACCACAACTCATCTGCCCAAGGAGATCAATAATGAAGGAGGCAAAAGAACTGATACAGATTTGAACATGGCAACAATGTCTGAGATTTTAAAGAACAATGAAAGGGTGGAACTCAGTTGTTTGATACTAAATAGAAGATCTTTTGCACAGACAGTGGAGAATCTATTTGCTCTGTCATTTCTGGCAAAAGACGGCAGAGTTAGAATTGCTGTCGAAGCAAATGGTTCTCATATTGTTTCTCCTACGAATGGTCCTGTTGCCAATGAAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

213

Amino Acids

24.45

Weight (kDa)

6.98

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 143 - 212 5.4e-18 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 58
AccBSI CCGCTC 1 cut(s) 78
AccIII TCCGGA 2 cut(s) 96, 136
AciI CCGC 2 cut(s) 76, 218
AcoI YGGCCR 1 cut(s) 46
AfaI GTAC 2 cut(s) 19, 313
AfiI CCNNNNNNNGG 3 cut(s) 55, 263, 569
AgsI TTSAA 2 cut(s) 385, 625
AleI CACNNNNGTG 1 cut(s) 250
AluBI AGCT 4 cut(s) 34, 73, 102, 199
AluI AGCT 4 cut(s) 34, 73, 102, 199
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 114
Aor13HI TCCGGA 2 cut(s) 96, 136
AoxI GGCC 2 cut(s) 46, 254
ApeKI GCWGC 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 266, 572
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 2 cut(s) 97, 590
AvaII GGWCC 2 cut(s) 266, 572
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 286
BceAI ACGGC 3 cut(s) 77, 80, 529
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 114
BglII AGATCT 1 cut(s) 458
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 2 cut(s) 266, 572
BmgT120I GGNCC 2 cut(s) 266, 572
BmiI GGNNCC 2 cut(s) 60, 268
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 1 cut(s) 211
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpuMI CCSGG 1 cut(s) 56
BsaJI CCNNGG 3 cut(s) 62, 248, 339
BsaWI WCCGGW 2 cut(s) 96, 136
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 263, 569
Bse1I ACTGG 2 cut(s) 108, 635
Bse3DI GCAATG 1 cut(s) 296
BseAI TCCGGA 2 cut(s) 96, 136
BseDI CCNNGG 3 cut(s) 62, 248, 339
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 3 cut(s) 55, 263, 569
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 2 cut(s) 393, 448
BseNI ACTGG 2 cut(s) 108, 635
BseXI GCAGC 1 cut(s) 286
BshFI GGCC 2 cut(s) 48, 256
BshNI GGYRCC 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 4 cut(s) 56, 97, 137, 307
BslI CCNNNNNNNGG 3 cut(s) 55, 263, 569
BsmAI GTCTC 1 cut(s) 114
BsmBI CGTCTC 1 cut(s) 114
BsnI GGCC 2 cut(s) 48, 256
Bsp1286I GDGCHC 1 cut(s) 75
Bsp13I TCCGGA 2 cut(s) 96, 136
Bsp143I GATC 3 cut(s) 159, 345, 458
BspACI CCGC 2 cut(s) 76, 218
BspANI GGCC 2 cut(s) 48, 256
BspCNI CTCAG 2 cut(s) 394, 447
BspEI TCCGGA 2 cut(s) 96, 136
BspLI GGNNCC 2 cut(s) 60, 268
BspT107I GGYRCC 1 cut(s) 58
BsrBI CCGCTC 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 296
BsrI ACTGG 2 cut(s) 108, 635
BssECI CCNNGG 3 cut(s) 62, 248, 339
BssMI GATC 3 cut(s) 159, 345, 458
BssT1I CCWWGG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 22, 249, 475
BstDEI CTNAG 3 cut(s) 6, 402, 434
BstDSI CCRYGG 2 cut(s) 62, 248
BstF5I GGATG 1 cut(s) 226
BstKTI GATC 3 cut(s) 162, 348, 461
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 3 cut(s) 159, 345, 458
BstMWI GCNNNNNNNGC 3 cut(s) 196, 316, 536
BstSCI CCNGG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 458
BstXI CCANNNNNNTGG 1 cut(s) 589
BstYI RGATCY 1 cut(s) 458
BsuRI GGCC 2 cut(s) 48, 256
BtgI CCRYGG 2 cut(s) 62, 248
BtsCI GGATG 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 115, 480
Cfr13I GGNCC 2 cut(s) 266, 572
Csp6I GTAC 2 cut(s) 18, 312
CviAII CATG 3 cut(s) 185, 271, 388
CviQI GTAC 2 cut(s) 18, 312
DdeI CTNAG 3 cut(s) 6, 402, 434
DpnI GATC 3 cut(s) 161, 347, 460
DpnII GATC 3 cut(s) 159, 345, 458
DraI TTTAAA 1 cut(s) 411
EaeI YGGCCR 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 266, 572
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 3 cut(s) 188, 274, 391
FaiI YATR 5 cut(s) 170, 186, 272, 389, 552
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 3 cut(s) 184, 270, 387
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 300
GluI GCNGC 1 cut(s) 300
HaeIII GGCC 2 cut(s) 48, 256
HapII CCGG 4 cut(s) 56, 97, 137, 307
Hin1II CATG 3 cut(s) 188, 274, 391
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 3 cut(s) 133, 140, 481
HpaII CCGG 4 cut(s) 56, 97, 137, 307
HphI GGTGA 2 cut(s) 97, 590
Hpy166II GTNNAC 2 cut(s) 18, 175
Hpy188I TCNGA 5 cut(s) 82, 204, 282, 403, 614
Hpy188III TCNNGA 2 cut(s) 97, 137
Hpy8I GTNNAC 2 cut(s) 18, 175
HpyAV CCTTC 3 cut(s) 289, 350, 628
HpyCH4III ACNGT 3 cut(s) 22, 249, 475
HpyCH4V TGCA 3 cut(s) 184, 224, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 316, 536
HpyF3I CTNAG 3 cut(s) 6, 402, 434
Hsp92II CATG 3 cut(s) 188, 274, 391
Kpn2I TCCGGA 2 cut(s) 96, 136
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 345, 458
LmnI GCTCC 3 cut(s) 78, 83, 99
LpnPI CCDG 8 cut(s) 69, 110, 121, 150, 320, 488, 588, 616
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 211
MaeIII GTNAC 1 cut(s) 241
MalI GATC 3 cut(s) 161, 347, 460
MbiI CCGCTC 1 cut(s) 78
MboI GATC 3 cut(s) 159, 345, 458
MboII GAAGA 1 cut(s) 468
MflI RGATCY 1 cut(s) 458
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 2 cut(s) 25, 525
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 3 cut(s) 98, 267, 353
MroI TCCGGA 2 cut(s) 96, 136
MseI TTAA 3 cut(s) 128, 285, 410
MslI CAYNNNNRTG 1 cut(s) 250
MspI CCGG 4 cut(s) 56, 97, 137, 307
MspR9I CCNGG 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 196, 316, 536
NciI CCSGG 1 cut(s) 56
NdeII GATC 3 cut(s) 159, 345, 458
NlaIII CATG 3 cut(s) 188, 274, 391
NlaIV GGNNCC 2 cut(s) 60, 268
OliI CACNNNNGTG 1 cut(s) 250
PfeI GAWTC 2 cut(s) 140, 481
PkrI GCNGC 1 cut(s) 301
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 60, 268
PspPI GGNCC 2 cut(s) 266, 572
PsuI RGATCY 1 cut(s) 458
RsaI GTAC 2 cut(s) 19, 313
RsaNI GTAC 2 cut(s) 18, 312
RseI CAYNNNNRTG 1 cut(s) 250
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 3 cut(s) 128, 285, 410
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 3 cut(s) 159, 345, 458
Sau96I GGNCC 2 cut(s) 266, 572
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 1 cut(s) 75
SetI ASST 6 cut(s) 36, 75, 90, 104, 201, 639
SfaNI GCATC 1 cut(s) 211
SinI GGWCC 2 cut(s) 266, 572
SmiMI CAYNNNNRTG 1 cut(s) 250
Sse9I AATT 2 cut(s) 25, 525
SsiI CCGC 2 cut(s) 76, 218
SspI AATATT 1 cut(s) 277
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 3 cut(s) 22, 249, 475
TaqI TCGA 2 cut(s) 143, 534
TasI AATT 2 cut(s) 25, 525
TatI WGTACW 1 cut(s) 17
TfiI GAWTC 2 cut(s) 140, 481
Tru1I TTAA 3 cut(s) 128, 285, 410
Tru9I TTAA 3 cut(s) 128, 285, 410
TscAI CASTG 2 cut(s) 115, 480
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 5 cut(s) 161, 287, 369, 435, 600
TspRI CASTG 2 cut(s) 115, 480
VpaK11BI GGWCC 2 cut(s) 266, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.