RLG00000036860

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000940
Physical Location & Seq
Forward (+)
85913270 .. 85915241
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036860

Sequence Viewer

Length: 1098 bp
ATGGCCGAGAAAGAGTGTACTGTCAATTTTGAGCTACTCAACTTCGGCCAAGACCCGGTGCCACGGCGAGAGCTCCGCTCCGACTACCTCGCCTTCCGGAGCTTCACCAGTGAGAAAAGAGACGATTTAATGAGTCCGGATTCGATGAAGTTTGATGTGATCATTGATATAGTTGACAAACTGCATGAGCAAGTTCAGCTTCCGAGAGAACAAGTAGCGGATGCACAAGCACTTCTGGGCATAAGCAACACAGTGGTGGCCTCTGTTATGTCACATTCTAATTGGGAAACTACCCCAAGCGATTTCATTACTGGTTTGATTACACATTTCGGGCAAGTCAATAGGCCATCAGTGAACAAAGATATTGCCCATGTATCGCTTAAATGGAAAGATATTGGACTTTCTGTTTCACCAATACTCGAGGGCTGTTGGATGCTTGGACCCATGAATATTGAGATTAAGCAAAGGAAGGCTGCAGTCCGGCGTACAAATCCTACCACAACTCATCTGCCCAAGGAGATCAATAATGGAGGCGGCAAAAGAACTGATACAGACTTGAACATGGCAACAATGTTTGAGATTTTAAAGAACAAGAAAAGGGTGGAACTCAGTTGTTTGATACTAAATAGAAGATCTTTTGCACAGACAGTGGAGAATCTATTTGCTCTGTCATTTCTGGCAAAAGACGGCAGAGTTAGAATTGCTGTCGAAGCAAATGGTTCTCATATTGTTTCTCCTACGAATGGTCCTGTTGCCAATGAAGTGTCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATAATGATGGTAATGGTGGCTGTTGGTGAGGAGCTGATGCCTCATAGAGACAGCACAAAGTGCACACCTGCCTCTGAGGAGCAACCAGCAGCATTCAATTCCCAAACAGCGACAGCCTTACACACAACCCGGATTCAAAAACTTACCAGAAACCATGCCCTGGTTGTGCAGGAAAAATCTACTGCAGAAGAATTCTTTGAAAATGATGATGCTGTTGGAGCCACAGAACTTCGCAGGTGTAAGCGCAGGTTTTCGTTTAATACATGTTTAAGCGGAAGCTTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

366

Amino Acids

41.13

Weight (kDa)

7.17

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 201 - 283 1.7e-19 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 886, 1035
Acc36I ACCTGC 3 cut(s) 886, 1035, 1047
AccB1I GGYRCC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 970
AccBSI CCGCTC 1 cut(s) 78
AccIII TCCGGA 2 cut(s) 96, 136
AciI CCGC 4 cut(s) 76, 218, 534, 1083
AcoI YGGCCR 2 cut(s) 3, 46
AcsI RAATTY 1 cut(s) 1001
AdeI CACNNNGTG 1 cut(s) 870
AfaI GTAC 2 cut(s) 19, 487
AfiI CCNNNNNNNGG 3 cut(s) 55, 743, 970
AflII CTTAAG 1 cut(s) 1090
AflIII ACRYGT 1 cut(s) 1073
AgsI TTSAA 5 cut(s) 559, 799, 907, 947, 1010
AjnI CCWGG 1 cut(s) 969
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 6 cut(s) 34, 73, 102, 199, 844, 1089
AluI AGCT 6 cut(s) 34, 73, 102, 199, 844, 1089
Alw21I GWGCWC 2 cut(s) 75, 875
Alw26I GTCTC 2 cut(s) 114, 852
Alw44I GTGCAC 1 cut(s) 871
Ama87I CYCGRG 1 cut(s) 419
Aor13HI TCCGGA 2 cut(s) 96, 136
AoxI GGCC 4 cut(s) 3, 46, 258, 344
ApaLI GTGCAC 1 cut(s) 871
ApeKI GCWGC 2 cut(s) 473, 899
ApoI RAATTY 1 cut(s) 1001
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 2 cut(s) 440, 746
AsuC2I CCSGG 2 cut(s) 56, 940
AsuHPI GGTGA 4 cut(s) 97, 402, 764, 848
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 2 cut(s) 440, 746
BaeGI GKGCMC 1 cut(s) 875
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 75, 875
BbvI GCAGC 2 cut(s) 460, 911
BccI CCATC 2 cut(s) 355, 811
BceAI ACGGC 2 cut(s) 80, 703
BciT130I CCWGG 1 cut(s) 971
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 2 cut(s) 56, 940
BcoDI GTCTC 2 cut(s) 114, 852
BfmI CTRYAG 2 cut(s) 474, 993
BfrI CTTAAG 1 cut(s) 1090
BfuAI ACCTGC 3 cut(s) 886, 1035, 1047
BglII AGATCT 1 cut(s) 632
BisI GCNGC 3 cut(s) 474, 535, 900
BlsI GCNGC 3 cut(s) 475, 536, 901
Bme1390I CCNGG 3 cut(s) 56, 940, 971
Bme18I GGWCC 2 cut(s) 440, 746
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 2 cut(s) 440, 746
BmiI GGNNCC 3 cut(s) 60, 442, 1030
BmrFI CCNGG 3 cut(s) 56, 940, 971
BmsI GCATC 4 cut(s) 211, 423, 837, 1009
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpuMI CCSGG 2 cut(s) 56, 940
BsaJI CCNNGG 3 cut(s) 62, 513, 969
BsaWI WCCGGW 2 cut(s) 96, 136
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 743, 970
Bse1I ACTGG 3 cut(s) 108, 316, 809
BseAI TCCGGA 2 cut(s) 96, 136
BseBI CCWGG 1 cut(s) 971
BseDI CCNNGG 3 cut(s) 62, 513, 969
BseGI GGATG 2 cut(s) 226, 438
BseLI CCNNNNNNNGG 3 cut(s) 55, 743, 970
BseMII CTCAG 2 cut(s) 622, 876
BseNI ACTGG 3 cut(s) 108, 316, 809
BseRI GAGGAG 2 cut(s) 854, 902
BseSI GKGCMC 1 cut(s) 875
BseXI GCAGC 2 cut(s) 460, 911
BsgI GTGCAG 1 cut(s) 998
BshFI GGCC 4 cut(s) 5, 48, 260, 346
BshNI GGYRCC 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 75, 875
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 5 cut(s) 56, 97, 137, 481, 940
BslI CCNNNNNNNGG 3 cut(s) 55, 743, 970
BsmAI GTCTC 2 cut(s) 114, 852
BsmBI CGTCTC 1 cut(s) 114
BsmI GAATGC 1 cut(s) 902
BsnI GGCC 4 cut(s) 5, 48, 260, 346
BsoBI CYCGRG 1 cut(s) 419
Bsp1286I GDGCHC 2 cut(s) 75, 875
Bsp13I TCCGGA 2 cut(s) 96, 136
Bsp143I GATC 3 cut(s) 159, 519, 632
BspACI CCGC 4 cut(s) 76, 218, 534, 1083
BspANI GGCC 4 cut(s) 5, 48, 260, 346
BspCNI CTCAG 2 cut(s) 621, 877
BspEI TCCGGA 2 cut(s) 96, 136
BspLI GGNNCC 3 cut(s) 60, 442, 1030
BspMAI CTGCAG 2 cut(s) 478, 997
BspMI ACCTGC 3 cut(s) 886, 1035, 1047
BspT107I GGYRCC 1 cut(s) 58
BspTI CTTAAG 1 cut(s) 1090
BsrBI CCGCTC 1 cut(s) 78
BsrI ACTGG 3 cut(s) 108, 316, 809
BssECI CCNNGG 3 cut(s) 62, 513, 969
BssMI GATC 3 cut(s) 159, 519, 632
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 971
Bst4CI ACNGT 3 cut(s) 22, 253, 649
BstAFI CTTAAG 1 cut(s) 1090
BstAPI GCANNNNNTGC 1 cut(s) 870
BstDEI CTNAG 2 cut(s) 608, 885
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 226, 438
BstHHI GCGC 1 cut(s) 1056
BstKTI GATC 3 cut(s) 162, 522, 635
BstMAI GTCTC 2 cut(s) 114, 852
BstMBI GATC 3 cut(s) 159, 519, 632
BstMWI GCNNNNNNNGC 4 cut(s) 196, 710, 870, 1028
BstNI CCWGG 1 cut(s) 971
BstNSI RCATGY 1 cut(s) 1077
BstSCI CCNGG 3 cut(s) 54, 938, 969
BstSFI CTRYAG 2 cut(s) 474, 993
BstSLI GKGCMC 1 cut(s) 875
BstV1I GCAGC 2 cut(s) 460, 911
BstX2I RGATCY 1 cut(s) 632
BstYI RGATCY 1 cut(s) 632
BsuRI GGCC 4 cut(s) 5, 48, 260, 346
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 226, 438
BtsIMutI CAGTG 4 cut(s) 115, 258, 357, 654
BveI ACCTGC 3 cut(s) 886, 1035, 1047
CfoI GCGC 1 cut(s) 1056
Cfr13I GGNCC 2 cut(s) 440, 746
Csp6I GTAC 2 cut(s) 18, 486
CviAII CATG 6 cut(s) 185, 371, 445, 562, 965, 1074
CviQI GTAC 2 cut(s) 18, 486
DdeI CTNAG 2 cut(s) 608, 885
DpnI GATC 3 cut(s) 161, 521, 634
DpnII GATC 3 cut(s) 159, 519, 632
DraI TTTAAA 1 cut(s) 585
DraIII CACNNNGTG 1 cut(s) 870
EaeI YGGCCR 2 cut(s) 3, 46
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 440, 746
Eco53kI GAGCTC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 1001
EcoRII CCWGG 1 cut(s) 969
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 513
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 6 cut(s) 188, 374, 448, 565, 968, 1077
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 6 cut(s) 184, 370, 444, 561, 964, 1073
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 474, 535, 900
FokI GGATG 2 cut(s) 233, 445
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 3 cut(s) 474, 535, 900
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 3 cut(s) 474, 535, 900
HaeIII GGCC 4 cut(s) 5, 48, 260, 346
HapII CCGG 5 cut(s) 56, 97, 137, 481, 940
HhaI GCGC 1 cut(s) 1056
Hin1II CATG 6 cut(s) 188, 374, 448, 565, 968, 1077
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 1087
HinfI GANTC 4 cut(s) 133, 140, 655, 943
HpaII CCGG 5 cut(s) 56, 97, 137, 481, 940
HphI GGTGA 4 cut(s) 97, 402, 764, 848
Hpy166II GTNNAC 4 cut(s) 18, 175, 355, 873
Hpy188I TCNGA 4 cut(s) 82, 204, 788, 886
Hpy188III TCNNGA 2 cut(s) 97, 137
Hpy8I GTNNAC 4 cut(s) 18, 175, 355, 873
HpyAV CCTTC 2 cut(s) 103, 463
HpyCH4III ACNGT 3 cut(s) 22, 253, 649
HpyCH4V TGCA 7 cut(s) 184, 224, 476, 641, 873, 979, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 710, 870, 1028
HpyF3I CTNAG 2 cut(s) 608, 885
Hsp92II CATG 6 cut(s) 188, 374, 448, 565, 968, 1077
HspAI GCGC 1 cut(s) 1054
Kpn2I TCCGGA 2 cut(s) 96, 136
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 519, 632
LmnI GCTCC 6 cut(s) 78, 83, 99, 841, 889, 1028
Lsp1109I GCAGC 2 cut(s) 460, 911
LweI GCATC 4 cut(s) 211, 423, 837, 1009
MaeIII GTNAC 1 cut(s) 270
MalI GATC 3 cut(s) 161, 521, 634
MbiI CCGCTC 1 cut(s) 78
MboI GATC 3 cut(s) 159, 519, 632
MboII GAAGA 3 cut(s) 642, 820, 1010
MflI RGATCY 1 cut(s) 632
MhlI GDGCHC 2 cut(s) 75, 875
MluCI AATT 5 cut(s) 25, 280, 699, 907, 1001
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 3 cut(s) 105, 410, 1006
MnlI CCTC 8 cut(s) 98, 271, 415, 524, 832, 861, 880, 892
MroI TCCGGA 2 cut(s) 96, 136
MseI TTAA 7 cut(s) 128, 381, 459, 584, 1068, 1079, 1091
MslI CAYNNNNRTG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 1090
MspI CCGG 5 cut(s) 56, 97, 137, 481, 940
MspR9I CCNGG 3 cut(s) 56, 940, 971
Mva1269I GAATGC 1 cut(s) 902
MvaI CCWGG 1 cut(s) 971
MwoI GCNNNNNNNGC 4 cut(s) 196, 710, 870, 1028
NciI CCSGG 2 cut(s) 56, 940
NdeII GATC 3 cut(s) 159, 519, 632
NlaIII CATG 6 cut(s) 188, 374, 448, 565, 968, 1077
NlaIV GGNNCC 3 cut(s) 60, 442, 1030
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 270
NspI RCATGY 1 cut(s) 1077
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 419
PaqCI CACCTGC 2 cut(s) 886, 1035
PciI ACATGT 1 cut(s) 1073
PctI GAATGC 1 cut(s) 902
PfeI GAWTC 3 cut(s) 140, 655, 943
PflMI CCANNNNNTGG 1 cut(s) 970
PkrI GCNGC 3 cut(s) 475, 536, 901
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
PscI ACATGT 1 cut(s) 1073
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 969
PspGI CCWGG 1 cut(s) 969
PspN4I GGNNCC 3 cut(s) 60, 442, 1030
PspPI GGNCC 2 cut(s) 440, 746
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 2 cut(s) 478, 997
PsuI RGATCY 1 cut(s) 632
RsaI GTAC 2 cut(s) 19, 487
RsaNI GTAC 2 cut(s) 18, 486
RseI CAYNNNNRTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 7 cut(s) 128, 381, 459, 584, 1068, 1079, 1091
SatI GCNGC 3 cut(s) 474, 535, 900
Sau3AI GATC 3 cut(s) 159, 519, 632
Sau96I GGNCC 2 cut(s) 440, 746
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 3 cut(s) 56, 940, 971
SduI GDGCHC 2 cut(s) 75, 875
SfaNI GCATC 4 cut(s) 211, 423, 837, 1009
SfcI CTRYAG 2 cut(s) 474, 993
Sfr274I CTCGAG 1 cut(s) 419
SinI GGWCC 2 cut(s) 440, 746
SlaI CTCGAG 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 419, 1090
SmoI CTYRAG 2 cut(s) 419, 1090
Sse9I AATT 5 cut(s) 25, 280, 699, 907, 1001
SsiI CCGC 4 cut(s) 76, 218, 534, 1083
SspI AATATT 1 cut(s) 451
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 3 cut(s) 54, 938, 969
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 3 cut(s) 22, 253, 649
TaqI TCGA 3 cut(s) 143, 420, 708
TasI AATT 5 cut(s) 25, 280, 699, 907, 1001
TatI WGTACW 1 cut(s) 17
TauI GCSGC 1 cut(s) 537
TfiI GAWTC 3 cut(s) 140, 655, 943
Tru1I TTAA 7 cut(s) 128, 381, 459, 584, 1068, 1079, 1091
Tru9I TTAA 7 cut(s) 128, 381, 459, 584, 1068, 1079, 1091
TscAI CASTG 4 cut(s) 115, 258, 357, 654
TseFI GTSAC 1 cut(s) 270
TseI GCWGC 2 cut(s) 473, 899
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 4 cut(s) 161, 295, 461, 774
TspRI CASTG 4 cut(s) 115, 258, 357, 654
Van91I CCANNNNNTGG 1 cut(s) 970
Vha464I CTTAAG 1 cut(s) 1090
VneI GTGCAC 1 cut(s) 871
VpaK11BI GGWCC 2 cut(s) 440, 746
XapI RAATTY 1 cut(s) 1001
XceI RCATGY 1 cut(s) 1077
XhoI CTCGAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.