Rorug05G0469200

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000940
Physical Location & Seq
Forward (+)
64607985 .. 64611692
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0469200.1

Sequence Viewer

Length: 1044 bp
ATGTCTCAAGATTCAGGGTTGAGAGCGGTGTGTCACGGTTCAGAAATAATGAATATTAGAATGCTGCAAACCAACGCACGTGGCTACCTTGAAAAGGCATTGTCGGAGCAGGAAAAGGCATTCTTAGAGCAGGATGAAGCCTTGAAAGTATCCGAAGCTCAGAAGAAAGATTTGAAGAAACAGCTTGAGGAGAGTGAAGCTCAGAACAAAGTGCTTAAGAAACAGCTTGAGCAGAGTGTTTCTCGGAATAAAGTGCTACAACAAAGGAATAACAGCATTATGTTGGAGAAGAAGAAGGCAGATGAAAAGGTGTTACTGCTTTCAGAAGAACATAAGAGAGAAAAGAATGAATTGTTTAGAACAATCATTGAATTGGAAAAGCAGCTTGACGTCAAAACTGCATTGGTGCTAGAGATTGAGCGTATAAGAGGTGCTTTATTGTTAATCAAACAAATGCATGAAGATGGGGATTTGCAAGGGCGAAATAAGATGAATAAGATTGAGGAACTGTTAAAGAAGAAGGAAGAGGACTTGACCATGGTAGAACTTATCATCAAGGAGTGGATAAGTAATGATGAAATTCAGGAAGCTCAGAAGGAGTTGATCGTTGGACTGTGGGATTCATCAAACCAGGCTTTTATTCATGTGAAGAGAGTGGGAGTTGACATCAATCCATTTCAGACTGCAACCAGCAGAAATTTTTTCAAAGAAGCAAATATTGAATTGTTGGAGTTGTGGTCTTTATGGGCCGATTATACTAGGGACGAGAGCTGGCACCCATTCAAAGTTATTGTGGATAAAGTAGGAAATGCAAAGGAAATTATTGATGAGAAGGATGCCACATTGAAATTTATAATGAACGAGTTTGATGAAGAATTTTACAAGTTTGTCACAACTGCCTTGGTCAAAGTGAACGAGAATGAGAACAAGAATGATCCCAGTTATAATCATATAAGACCCGTGATGTGGAACTGTAAGACAGGGAGGAGAGCTACAGTTAGAGATGTATATTTGAAAGCGGTGGATTGGTGGAACAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

347

Amino Acids

40.71

Weight (kDa)

5.89

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XH PF03469 220 - 344 6.6e-24 XH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 854, 945
AatII GACGTC 1 cut(s) 393
AccB1I GGYRCC 1 cut(s) 774
AccBSI CCGCTC 1 cut(s) 26
AciI CCGC 2 cut(s) 26, 1019
AclWI GGATC 1 cut(s) 929
AcsI RAATTY 4 cut(s) 579, 697, 848, 875
AcvI CACGTG 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 390
AfiI CCNNNNNNNGG 2 cut(s) 94, 966
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 9 cut(s) 92, 145, 175, 371, 706, 722, 784, 847, 1015
AjnI CCWGG 1 cut(s) 630
AluBI AGCT 8 cut(s) 158, 184, 200, 226, 385, 590, 771, 992
AluI AGCT 8 cut(s) 158, 184, 200, 226, 385, 590, 771, 992
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 929
AoxI GGCC 1 cut(s) 747
ApeKI GCWGC 2 cut(s) 64, 382
ApoI RAATTY 4 cut(s) 579, 697, 848, 875
AspS9I GGNCC 1 cut(s) 747
BanI GGYRCC 1 cut(s) 774
BbrPI CACGTG 1 cut(s) 80
BbvI GCAGC 2 cut(s) 51, 394
BccI CCATC 1 cut(s) 458
BciT130I CCWGG 1 cut(s) 632
BciVI GTATCC 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 410, 759
BfmI CTRYAG 1 cut(s) 993
BfrI CTTAAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 160
BisI GCNGC 2 cut(s) 65, 383
BlsI GCNGC 2 cut(s) 66, 384
Bme1390I CCNGG 1 cut(s) 632
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 1 cut(s) 776
BmrFI CCNGG 1 cut(s) 632
BmrI ACTGGG 1 cut(s) 933
BmsI GCATC 1 cut(s) 826
BmuI ACTGGG 1 cut(s) 933
BplI GAGNNNNNCTC 4 cut(s) 184, 216, 226, 258
BpuEI CTTGAG 2 cut(s) 206, 248
BsaAI YACGTR 1 cut(s) 80
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 537, 900
BsaXI ACNNNNNCTCC 2 cut(s) 722, 752
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 966
Bse1I ACTGG 1 cut(s) 939
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 2 cut(s) 537, 900
BseGI GGATG 2 cut(s) 139, 841
BseLI CCNNNNNNNGG 2 cut(s) 94, 966
BseMII CTCAG 3 cut(s) 173, 215, 605
BseNI ACTGG 1 cut(s) 939
BseRI GAGGAG 2 cut(s) 203, 1000
BseXI GCAGC 2 cut(s) 51, 394
BshFI GGCC 1 cut(s) 749
BshNI GGYRCC 1 cut(s) 774
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 2 cut(s) 94, 966
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 776
BsmI GAATGC 2 cut(s) 66, 119
BsnI GGCC 1 cut(s) 749
Bsp143I GATC 2 cut(s) 603, 934
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 2 cut(s) 26, 1019
BspANI GGCC 1 cut(s) 749
BspCNI CTCAG 3 cut(s) 172, 214, 604
BspLI GGNNCC 1 cut(s) 776
BspPI GGATC 1 cut(s) 929
BspT107I GGYRCC 1 cut(s) 774
BspTI CTTAAG 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 26
BsrI ACTGG 1 cut(s) 939
BssECI CCNNGG 2 cut(s) 537, 900
BssMI GATC 2 cut(s) 603, 934
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 2 cut(s) 537, 900
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 5 cut(s) 38, 510, 615, 974, 997
Bst6I CTCTTC 2 cut(s) 519, 644
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 773
BstDEI CTNAG 4 cut(s) 124, 159, 201, 591
BstDSI CCRYGG 1 cut(s) 537
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 2 cut(s) 139, 841
BstKTI GATC 2 cut(s) 606, 937
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 603, 934
BstNI CCWGG 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 630
BstSFI CTRYAG 1 cut(s) 993
BstV1I GCAGC 2 cut(s) 51, 394
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 749
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 2 cut(s) 139, 841
Cac8I GCNNGC 1 cut(s) 773
Cfr13I GGNCC 1 cut(s) 747
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 3 cut(s) 458, 538, 644
DdeI CTNAG 4 cut(s) 124, 159, 201, 591
DpnI GATC 2 cut(s) 605, 936
DpnII GATC 2 cut(s) 603, 934
Eam1104I CTCTTC 2 cut(s) 519, 644
EarI CTCTTC 2 cut(s) 519, 644
Eco130I CCWWGG 2 cut(s) 537, 900
Eco72I CACGTG 1 cut(s) 80
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 630
EcoT14I CCWWGG 2 cut(s) 537, 900
EcoT22I ATGCAT 1 cut(s) 459
ErhI CCWWGG 2 cut(s) 537, 900
FaeI CATG 3 cut(s) 461, 541, 647
FalI AAGNNNNNCTT 4 cut(s) 107, 139, 418, 450
FaqI GGGAC 1 cut(s) 776
FatI CATG 3 cut(s) 457, 537, 643
Fnu4HI GCNGC 2 cut(s) 65, 383
FokI GGATG 2 cut(s) 146, 848
Fsp4HI GCNGC 2 cut(s) 65, 383
FspBI CTAG 2 cut(s) 410, 759
GluI GCNGC 2 cut(s) 65, 383
HaeIII GGCC 1 cut(s) 749
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 3 cut(s) 461, 541, 647
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 2 cut(s) 11, 620
Hpy166II GTNNAC 2 cut(s) 664, 913
Hpy188I TCNGA 9 cut(s) 43, 106, 154, 162, 204, 246, 325, 594, 681
Hpy188III TCNNGA 2 cut(s) 8, 584
Hpy8I GTNNAC 2 cut(s) 664, 913
HpyAV CCTTC 4 cut(s) 289, 514, 589, 826
HpyCH4III ACNGT 5 cut(s) 38, 510, 615, 974, 997
HpyCH4IV ACGT 2 cut(s) 79, 390
HpyCH4V TGCA 6 cut(s) 67, 401, 457, 475, 686, 812
HpyF3I CTNAG 4 cut(s) 124, 159, 201, 591
HpySE526I ACGT 2 cut(s) 79, 390
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 3 cut(s) 461, 541, 647
Kzo9I GATC 2 cut(s) 603, 934
LmnI GCTCC 1 cut(s) 106
LpnPI CCDG 9 cut(s) 95, 116, 569, 617, 644, 703, 757, 952, 966
Lsp1109I GCAGC 2 cut(s) 51, 394
LweI GCATC 1 cut(s) 826
MaeI CTAG 2 cut(s) 410, 759
MaeII ACGT 2 cut(s) 79, 390
MaeIII GTNAC 3 cut(s) 32, 312, 889
MalI GATC 2 cut(s) 605, 936
MbiI CCGCTC 1 cut(s) 26
MboI GATC 2 cut(s) 603, 934
MluCI AATT 8 cut(s) 350, 371, 579, 697, 722, 819, 848, 875
MmeI TCCRAC 4 cut(s) 84, 264, 589, 708
MnlI CCTC 5 cut(s) 181, 422, 496, 520, 978
Mph1103I ATGCAT 1 cut(s) 459
MseI TTAA 3 cut(s) 216, 443, 512
MslI CAYNNNNRTG 1 cut(s) 462
MspCI CTTAAG 1 cut(s) 215
MspR9I CCNGG 1 cut(s) 632
Mva1269I GAATGC 2 cut(s) 66, 119
MvaI CCWGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 537
NdeII GATC 2 cut(s) 603, 934
NlaIII CATG 3 cut(s) 461, 541, 647
NlaIV GGNNCC 1 cut(s) 776
NmuCI GTSAC 2 cut(s) 32, 889
NsiI ATGCAT 1 cut(s) 459
PctI GAATGC 2 cut(s) 66, 119
PfeI GAWTC 2 cut(s) 11, 620
PkrI GCNGC 2 cut(s) 66, 384
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 80
PsiI TTATAA 2 cut(s) 854, 945
Psp6I CCWGG 1 cut(s) 630
PspCI CACGTG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 1 cut(s) 776
PspPI GGNCC 1 cut(s) 747
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 3 cut(s) 216, 443, 512
SatI GCNGC 2 cut(s) 65, 383
Sau3AI GATC 2 cut(s) 603, 934
Sau96I GGNCC 1 cut(s) 747
ScrFI CCNGG 1 cut(s) 632
SfaNI GCATC 1 cut(s) 826
SfcI CTRYAG 1 cut(s) 993
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 4 cut(s) 6, 185, 215, 227
SmoI CTYRAG 4 cut(s) 6, 185, 215, 227
Sse9I AATT 8 cut(s) 350, 371, 579, 697, 722, 819, 848, 875
SsiI CCGC 2 cut(s) 26, 1019
SspI AATATT 2 cut(s) 55, 718
SspMI CTAG 2 cut(s) 410, 759
StyD4I CCNGG 1 cut(s) 630
StyI CCWWGG 2 cut(s) 537, 900
TaaI ACNGT 5 cut(s) 38, 510, 615, 974, 997
TaiI ACGT 2 cut(s) 82, 393
TasI AATT 8 cut(s) 350, 371, 579, 697, 722, 819, 848, 875
TfiI GAWTC 2 cut(s) 11, 620
Tru1I TTAA 3 cut(s) 216, 443, 512
Tru9I TTAA 3 cut(s) 216, 443, 512
TseFI GTSAC 2 cut(s) 32, 889
TseI GCWGC 2 cut(s) 64, 382
Tsp45I GTSAC 2 cut(s) 32, 889
Vha464I CTTAAG 1 cut(s) 215
XagI CCTNNNNNAGG 1 cut(s) 92
XapI RAATTY 4 cut(s) 579, 697, 848, 875
XspI CTAG 2 cut(s) 410, 759
ZraI GACGTC 1 cut(s) 391
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.