Rw0G019230

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00864
OG0000940
Physical Location & Seq
Reverse (-)
59800 .. 61730
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019230.1

Sequence Viewer

Length: 1077 bp
ATGGTGAAGCGCGTATTGGGTTGTGCTACTAGTACTAGTAGAGGAGGAGATGAAACGCACGAAACTGATCACGCGACGCCGGCGCTGGCTGACCCTAGAGTACTCCGATCACGCTACCACACTGTCAAGACCATCCTCTACGATGAAAGAGAGGATCTCACAAGAGCAGATTCGGAGAAGTTCAATTCGATCATTAAGGAAGTGGAGAGCTTGCACCAGCTGGTAACAAAGCCAAGAGAGCAAGTCGCGGATGCGGAGGCGCTTTTGGACATTGCATGCACCCTGATGACCTCTGTACGGGCACATAACAAGGAAGGAATTACAGCTTCGGGTTTCGTCAACTGTATTCTTAGTGACTTCAGGAAACCGAGTGGTGATGGCAGCAGCTCTATATCTTGGAGGGACATTGGGAATGCGGTATCACATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATTGGGCCAATGAATGCTGATGTGAAGCAACGAAAGGTTTATCAACGTCAAAAACGTGGGAATCCTACACACAATGATACCCCTAAAGAGCTTGGTGATGCTGTTGTTGAAGAGGAAACCGAGACTGTTAAAAATGTGGCAACTATGTTTAATATTTTGAAGAAAAAGAGAAGGGTCAGGCTTGAATATCTAGTTTTGAATGGAAATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCGTTTCTTGTTAAAGATGGGCGAGTCGAAATAAAAGTGAACGAGGAAGGCCACCATCTAGTGTCGCCAAGGAATGCTCCTGCTGCTGAAGCAATTGCCTCAGGCGCTGTTACTTACTTTGTTGGGAATGGGGAGGAGCTGATGCCACATAGGTTTCCAGCTGACGATGTATCAAACAATTCGCAACTGGAAGAACCTCAAGCAACAACAGGTACAACACCAATTAGGAAGTTCTCAAGGAACAGAGGCTTGGTTATAAGGGAGCAGCCCGTTGGGACTGATAGTAGCAGCAGATCAATTGCTGAAGACAGCGCTGAATGTGATTATGCTGCAAGCGCTTCTGCCACTCGTAAAGGGAAGCGTAAACTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

358

Amino Acids

39.56

Weight (kDa)

8.16

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 212 - 264 2.7e-14 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
AccII CGCG 3 cut(s) 12, 74, 248
AciI CCGC 3 cut(s) 248, 254, 416
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 3 cut(s) 343, 810, 1026
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 34, 102, 297, 916
AfeI AGCGCT 2 cut(s) 1015, 1039
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 5 cut(s) 184, 569, 619, 644, 658
AhlI ACTAGT 2 cut(s) 29, 35
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 935, 967
AluBI AGCT 7 cut(s) 210, 220, 326, 387, 550, 841, 863
AluI AGCT 7 cut(s) 210, 220, 326, 387, 550, 841, 863
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 809
Aor51HI AGCGCT 2 cut(s) 1015, 1039
AoxI GGCC 2 cut(s) 464, 751
ApeKI GCWGC 7 cut(s) 381, 384, 450, 785, 967, 990, 1031
ApoI RAATTY 1 cut(s) 664
AspLEI GCGC 6 cut(s) 12, 85, 262, 809, 1016, 1040
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 3 cut(s) 16, 386, 566
AxyI CCTNAGG 1 cut(s) 802
BaeGI GKGCMC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 1014
BbvI GCAGC 7 cut(s) 393, 396, 437, 772, 979, 1002, 1018
BccI CCATC 4 cut(s) 140, 371, 713, 765
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 575
BcuI ACTAGT 2 cut(s) 29, 35
BfaI CTAG 5 cut(s) 30, 36, 96, 650, 761
BfoI RGCGCY 5 cut(s) 86, 263, 810, 1017, 1041
BisI GCNGC 7 cut(s) 382, 385, 451, 786, 968, 991, 1032
BlsI GCNGC 7 cut(s) 383, 386, 452, 787, 969, 992, 1033
BmcAI AGTACT 2 cut(s) 34, 102
BmgT120I GGNCC 1 cut(s) 464
BmsI GCATC 4 cut(s) 241, 437, 547, 834
BpiI GAAGAC 1 cut(s) 1014
BplI GAGNNNNNCTC 4 cut(s) 141, 173, 427, 459
BpuEI CTTGAG 2 cut(s) 885, 922
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 770
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 894
Bse21I CCTNAGG 1 cut(s) 802
Bse3DI GCAATG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 770
BseGI GGATG 2 cut(s) 132, 256
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 1 cut(s) 894
BseRI GAGGAG 3 cut(s) 57, 60, 851
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 7 cut(s) 393, 396, 437, 772, 979, 1002, 1018
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 3 cut(s) 12, 74, 248
BshFI GGCC 2 cut(s) 466, 753
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 2 cut(s) 416, 991
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 2 cut(s) 416, 991
BsmI GAATGC 3 cut(s) 418, 478, 781
BsnI GGCC 2 cut(s) 466, 753
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 5 cut(s) 67, 107, 154, 189, 995
BspACI CCGC 3 cut(s) 248, 254, 416
BspANI GGCC 2 cut(s) 466, 753
BspCNI CTCAG 1 cut(s) 815
BspFNI CGCG 3 cut(s) 12, 74, 248
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 894
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 770
BssMI GATC 5 cut(s) 67, 107, 154, 189, 995
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 770
Bst4CI ACNGT 4 cut(s) 124, 344, 586, 682
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 5 cut(s) 81, 87, 212, 277, 1036
BstDEI CTNAG 2 cut(s) 350, 802
BstF5I GGATG 2 cut(s) 132, 256
BstFNI CGCG 3 cut(s) 12, 74, 248
BstH2I RGCGCY 5 cut(s) 86, 263, 810, 1017, 1041
BstHHI GCGC 6 cut(s) 12, 85, 262, 809, 1016, 1040
BstKTI GATC 5 cut(s) 70, 110, 157, 192, 998
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 5 cut(s) 67, 107, 154, 189, 995
BstMWI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1037
BstNSI RCATGY 2 cut(s) 279, 428
BstSLI GKGCMC 1 cut(s) 304
BstUI CGCG 3 cut(s) 12, 74, 248
BstV1I GCAGC 7 cut(s) 393, 396, 437, 772, 979, 1002, 1018
BstV2I GAAGAC 1 cut(s) 1014
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
Bsu36I CCTNAGG 1 cut(s) 802
BsuRI GGCC 2 cut(s) 466, 753
BtsCI GGATG 2 cut(s) 132, 256
BtsIMutI CAGTG 2 cut(s) 120, 687
Cac8I GCNNGC 5 cut(s) 81, 87, 212, 277, 1036
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 6 cut(s) 12, 85, 262, 809, 1016, 1040
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 85
Csp6I GTAC 4 cut(s) 33, 101, 296, 915
CviAII CATG 2 cut(s) 276, 425
CviQI GTAC 4 cut(s) 33, 101, 296, 915
DdeI CTNAG 2 cut(s) 350, 802
DpnI GATC 5 cut(s) 69, 109, 156, 191, 997
DpnII GATC 5 cut(s) 67, 107, 154, 189, 995
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 770
Eco47III AGCGCT 2 cut(s) 1015, 1039
Eco57I CTGAAG 3 cut(s) 343, 810, 1026
Eco81I CCTNAGG 1 cut(s) 802
EcoT14I CCWWGG 1 cut(s) 770
ErhI CCWWGG 1 cut(s) 770
FaeI CATG 2 cut(s) 279, 428
FaqI GGGAC 2 cut(s) 416, 991
FatI CATG 2 cut(s) 275, 424
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 7 cut(s) 382, 385, 451, 786, 968, 991, 1032
FokI GGATG 2 cut(s) 119, 263
Fsp4HI GCNGC 7 cut(s) 382, 385, 451, 786, 968, 991, 1032
FspBI CTAG 5 cut(s) 30, 36, 96, 650, 761
GlaI GCGC 6 cut(s) 11, 84, 261, 808, 1015, 1039
GluI GCNGC 7 cut(s) 382, 385, 451, 786, 968, 991, 1032
HaeII RGCGCY 5 cut(s) 86, 263, 810, 1017, 1041
HaeIII GGCC 2 cut(s) 466, 753
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 85
HhaI GCGC 6 cut(s) 12, 85, 262, 809, 1016, 1040
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 2 cut(s) 279, 428
Hin6I GCGC 6 cut(s) 10, 83, 260, 807, 1014, 1038
HinP1I GCGC 6 cut(s) 10, 83, 260, 807, 1014, 1038
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 3 cut(s) 170, 520, 726
HpaII CCGG 1 cut(s) 80
HphI GGTGA 3 cut(s) 16, 386, 566
Hpy166II GTNNAC 3 cut(s) 340, 742, 1067
Hpy188I TCNGA 2 cut(s) 107, 175
Hpy188III TCNNGA 3 cut(s) 127, 361, 433
Hpy8I GTNNAC 3 cut(s) 340, 742, 1067
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 308, 624, 743
HpyCH4III ACNGT 4 cut(s) 124, 344, 586, 682
HpyCH4IV ACGT 2 cut(s) 505, 514
HpyCH4V TGCA 5 cut(s) 214, 275, 279, 453, 1034
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1037
HpyF3I CTNAG 2 cut(s) 350, 802
HpySE526I ACGT 2 cut(s) 505, 514
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 2 cut(s) 279, 428
HspAI GCGC 6 cut(s) 10, 83, 260, 807, 1014, 1038
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 107, 154, 189, 995
LmnI GCTCC 3 cut(s) 784, 838, 964
Lsp1109I GCAGC 7 cut(s) 393, 396, 437, 772, 979, 1002, 1018
LweI GCATC 4 cut(s) 241, 437, 547, 834
MaeI CTAG 5 cut(s) 30, 36, 96, 650, 761
MaeII ACGT 2 cut(s) 505, 514
MaeIII GTNAC 3 cut(s) 223, 353, 811
MalI GATC 5 cut(s) 69, 109, 156, 191, 997
MboI GATC 5 cut(s) 67, 107, 154, 189, 995
MboII GAAGA 4 cut(s) 581, 631, 905, 1019
MfeI CAATTG 2 cut(s) 795, 999
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 7 cut(s) 184, 318, 664, 795, 880, 924, 999
MlyI GAGTC 1 cut(s) 735
MreI CGCCGGCG 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 4 cut(s) 195, 588, 609, 714
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 220, 863
MspI CCGG 1 cut(s) 80
MunI CAATTG 2 cut(s) 795, 999
Mva1269I GAATGC 3 cut(s) 418, 478, 781
MvnI CGCG 3 cut(s) 12, 74, 248
MwoI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1037
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 5 cut(s) 67, 107, 154, 189, 995
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 2 cut(s) 279, 428
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 2 cut(s) 279, 428
PaeI GCATGC 1 cut(s) 279
PciI ACATGT 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PctI GAATGC 3 cut(s) 418, 478, 781
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 170, 520
PkrI GCNGC 7 cut(s) 383, 386, 452, 787, 969, 992, 1033
PleI GAGTC 1 cut(s) 734
PpsI GAGTC 1 cut(s) 734
PscI ACATGT 1 cut(s) 424
PsiI TTATAA 1 cut(s) 959
PspPI GGNCC 1 cut(s) 464
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 2 cut(s) 220, 863
RsaI GTAC 4 cut(s) 34, 102, 297, 916
RsaNI GTAC 4 cut(s) 33, 101, 296, 915
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 4 cut(s) 195, 588, 609, 714
SatI GCNGC 7 cut(s) 382, 385, 451, 786, 968, 991, 1032
Sau3AI GATC 5 cut(s) 67, 107, 154, 189, 995
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 2 cut(s) 34, 102
SchI GAGTC 1 cut(s) 735
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 4 cut(s) 241, 437, 547, 834
SgrAI CRCCGGYG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 900, 937
SmoI CTYRAG 2 cut(s) 900, 937
SpeI ACTAGT 2 cut(s) 29, 35
SphI GCATGC 1 cut(s) 279
Sse9I AATT 7 cut(s) 184, 318, 664, 795, 880, 924, 999
SsiI CCGC 3 cut(s) 248, 254, 416
SspI AATATT 1 cut(s) 613
SspMI CTAG 5 cut(s) 30, 36, 96, 650, 761
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 4 cut(s) 124, 344, 586, 682
TaiI ACGT 2 cut(s) 508, 517
TaqI TCGA 3 cut(s) 188, 445, 729
TasI AATT 7 cut(s) 184, 318, 664, 795, 880, 924, 999
TatI WGTACW 2 cut(s) 32, 100
TfiI GAWTC 2 cut(s) 170, 520
Tru1I TTAA 4 cut(s) 195, 588, 609, 714
Tru9I TTAA 4 cut(s) 195, 588, 609, 714
TscAI CASTG 2 cut(s) 127, 687
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 7 cut(s) 381, 384, 450, 785, 967, 990, 1031
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 66, 159, 485
TspRI CASTG 2 cut(s) 127, 687
XapI RAATTY 1 cut(s) 664
XceI RCATGY 2 cut(s) 279, 428
XspI CTAG 5 cut(s) 30, 36, 96, 650, 761
ZrmI AGTACT 2 cut(s) 34, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.