Rh3BG262600

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000940
Physical Location & Seq
Reverse (-)
25435285 .. 25437723
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG262600.1

Sequence Viewer

Length: 1152 bp
ATGGTGAAACGCGTATTGGGTTGTGCTACTAGTAGCGGAGGAGATGAAACGCACGAAACTGATCACGCGACGCCGGCGGTGGCTGACCCTAGAGTACTCCGATCACGCTACCACACTGTCAAGACCATCCTCTACGATGAAAGAGAGGATCTCACAAGAGCAGACTCGGAGAAGTTCAATTCGATCATTAAGGAAGTGGAGAGCTTGCACAAGCTGGTAACAAAGCCAAGAGAGCAAGTCGCGGATGCAGAGGCGCTTTTGGACATTGCAAGCACCCTGATGACCTCTGTACGGGCACATAACAAGGAAGGAATTACAGCTTCGTGTTTTGTCAACTGTATTCTTAGTGACTTCAGGAAACCGAGTGGTAATGGCAGCAGCTCAATATCGTGGAGGGACATTGGGAATGCAGTATCACATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATTGGGCCTATGAATGCTGATGTGAAGCGACGAAAGGTTTATCAACGTCAAAAACGTGGGAATCCTACAGACAATGATACCCCTAAAGAGCTTGGTGATGCTGTTGTAGAAGAGGAAACAGAGACTGTTAAAAATGTGGCAACTATGTTTAATATTTTGAAGAAAAAGAGAAGGGTCAGGCTTGAATATCTAGTTTTGAATGGAAATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCGTTTCTTGTTAAAGATGGGCGAGTCGAAATAAAAGTGAACGAGGAAGGCTATCATCTAGTGTCGCCAAGGAATGCTCCTGCTGCCAAAGCAATTGCCTCAGGCACTGTTACTTACTGCCACTTAATCTTCAGATTTGATTTCAAGGACTGGAAGTTAATGAAAGAAGTTGTTGGGAATGGGGAGGAGCTGATGCCACATAGGTTTCCAGCTGACGATGTATCAAACAATTCGCAACTGGAAGAACCTCAAGCAACAACAGGTACAACACCAATTAGGAAGTTCTCAAGGAACAGAGGGTTGGTTATACGGGAGCAGCCCGTTGGGACTGATAGCAGTAGCAGATCAATTGCTGAAGACAGCGCTGAATTTGATTATGCTTCAAGCGCTTCTGCCACTCGTAAAGGGAAGCGAGTACACTATGATGGAGGTCATGAAGTAGGGGCATTAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

383

Amino Acids

42.62

Weight (kDa)

8.13

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 210 - 297 3.4e-22 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 12, 68, 242
AciI CCGC 3 cut(s) 36, 77, 242
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 2 cut(s) 658, 1064
AcuI CTGAAG 3 cut(s) 337, 811, 1071
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 4 cut(s) 96, 291, 961, 1113
AfeI AGCGCT 2 cut(s) 1060, 1084
AfiI CCNNNNNNNGG 1 cut(s) 291
AflIII ACRYGT 2 cut(s) 10, 418
AgsI TTSAA 6 cut(s) 178, 613, 638, 652, 841, 1080
AhlI ACTAGT 1 cut(s) 29
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 980, 1012
AluBI AGCT 7 cut(s) 204, 214, 320, 381, 544, 886, 908
AluI AGCT 7 cut(s) 204, 214, 320, 381, 544, 886, 908
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 1 cut(s) 156
AlwNI CAGNNNCTG 2 cut(s) 578, 803
Aor51HI AGCGCT 2 cut(s) 1060, 1084
AoxI GGCC 1 cut(s) 458
ApeKI GCWGC 5 cut(s) 375, 378, 444, 779, 1012
ApoI RAATTY 2 cut(s) 658, 1064
AspLEI GCGC 3 cut(s) 256, 1061, 1085
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 16, 560
AxyI CCTNAGG 1 cut(s) 796
BaeGI GKGCMC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 1059
BbvI GCAGC 5 cut(s) 387, 390, 431, 766, 1024
BccI CCATC 3 cut(s) 134, 707, 1115
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 569
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 4 cut(s) 30, 90, 644, 755
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 3 cut(s) 257, 1062, 1086
BisI GCNGC 5 cut(s) 376, 379, 445, 780, 1013
BlsI GCNGC 5 cut(s) 377, 380, 446, 781, 1014
BmcAI AGTACT 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 458
BmsI GCATC 4 cut(s) 235, 431, 541, 879
BpiI GAAGAC 1 cut(s) 1059
BplI GAGNNNNNCTC 4 cut(s) 135, 167, 421, 453
BpuEI CTTGAG 2 cut(s) 930, 967
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 764
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse118I RCCGGY 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 851, 939
Bse21I CCTNAGG 1 cut(s) 796
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 2 cut(s) 126, 250
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 1 cut(s) 810
BseNI ACTGG 2 cut(s) 851, 939
BseRI GAGGAG 2 cut(s) 54, 896
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 5 cut(s) 387, 390, 431, 766, 1024
BsgI GTGCAG 1 cut(s) 430
Bsh1236I CGCG 3 cut(s) 12, 68, 242
BshFI GGCC 1 cut(s) 460
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 410, 1036
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 2 cut(s) 410, 1036
BsmI GAATGC 3 cut(s) 412, 472, 775
BsnI GGCC 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 5 cut(s) 61, 101, 148, 183, 1040
BspACI CCGC 3 cut(s) 36, 77, 242
BspANI GGCC 1 cut(s) 460
BspCNI CTCAG 1 cut(s) 809
BspFNI CGCG 3 cut(s) 12, 68, 242
BspHI TCATGA 1 cut(s) 1129
BspPI GGATC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 73
BsrI ACTGG 2 cut(s) 851, 939
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 5 cut(s) 61, 101, 148, 183, 1040
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 764
Bst4CI ACNGT 5 cut(s) 118, 338, 580, 676, 805
Bst6I CTCTTC 1 cut(s) 558
BstACI GRCGYC 1 cut(s) 71
BstC8I GCNNGC 3 cut(s) 75, 206, 271
BstDEI CTNAG 2 cut(s) 344, 796
BstF5I GGATG 2 cut(s) 126, 250
BstFNI CGCG 3 cut(s) 12, 68, 242
BstH2I RGCGCY 3 cut(s) 257, 1062, 1086
BstHHI GCGC 3 cut(s) 256, 1061, 1085
BstKTI GATC 5 cut(s) 64, 104, 151, 186, 1043
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 5 cut(s) 61, 101, 148, 183, 1040
BstMWI GCNNNNNNNGC 6 cut(s) 74, 232, 441, 779, 785, 1082
BstNSI RCATGY 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 3 cut(s) 12, 68, 242
BstV1I GCAGC 5 cut(s) 387, 390, 431, 766, 1024
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 796
BsuRI GGCC 1 cut(s) 460
BtsCI GGATG 2 cut(s) 126, 250
BtsIMutI CAGTG 3 cut(s) 114, 681, 801
Cac8I GCNNGC 3 cut(s) 75, 206, 271
CaiI CAGNNNCTG 2 cut(s) 578, 803
CciI TCATGA 1 cut(s) 1129
CfoI GCGC 3 cut(s) 256, 1061, 1085
Cfr10I RCCGGY 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 458
CseI GACGC 1 cut(s) 79
Csp6I GTAC 4 cut(s) 95, 290, 960, 1112
CviAII CATG 2 cut(s) 419, 1130
CviQI GTAC 4 cut(s) 95, 290, 960, 1112
DdeI CTNAG 2 cut(s) 344, 796
DpnI GATC 5 cut(s) 63, 103, 150, 185, 1042
DpnII GATC 5 cut(s) 61, 101, 148, 183, 1040
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 764
Eco47III AGCGCT 2 cut(s) 1060, 1084
Eco57I CTGAAG 3 cut(s) 337, 811, 1071
Eco81I CCTNAGG 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 764
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 2 cut(s) 422, 1133
FaqI GGGAC 2 cut(s) 410, 1036
FatI CATG 2 cut(s) 418, 1129
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 376, 379, 445, 780, 1013
FokI GGATG 2 cut(s) 113, 257
Fsp4HI GCNGC 5 cut(s) 376, 379, 445, 780, 1013
FspBI CTAG 4 cut(s) 30, 90, 644, 755
GlaI GCGC 3 cut(s) 255, 1060, 1084
GluI GCNGC 5 cut(s) 376, 379, 445, 780, 1013
HaeII RGCGCY 3 cut(s) 257, 1062, 1086
HaeIII GGCC 1 cut(s) 460
HapII CCGG 1 cut(s) 74
HgaI GACGC 1 cut(s) 79
HhaI GCGC 3 cut(s) 256, 1061, 1085
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 2 cut(s) 422, 1133
Hin6I GCGC 3 cut(s) 254, 1059, 1083
HinP1I GCGC 3 cut(s) 254, 1059, 1083
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 3 cut(s) 164, 514, 720
HpaII CCGG 1 cut(s) 74
HphI GGTGA 2 cut(s) 16, 560
Hpy166II GTNNAC 3 cut(s) 334, 736, 1114
Hpy188I TCNGA 3 cut(s) 101, 169, 830
Hpy188III TCNNGA 4 cut(s) 121, 355, 427, 1130
Hpy8I GTNNAC 3 cut(s) 334, 736, 1114
Hpy99I CGWCG 2 cut(s) 73, 486
HpyAV CCTTC 3 cut(s) 302, 618, 737
HpyCH4III ACNGT 5 cut(s) 118, 338, 580, 676, 805
HpyCH4IV ACGT 2 cut(s) 499, 508
HpyCH4V TGCA 5 cut(s) 208, 248, 269, 410, 447
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 232, 441, 779, 785, 1082
HpyF3I CTNAG 2 cut(s) 344, 796
HpySE526I ACGT 2 cut(s) 499, 508
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 2 cut(s) 422, 1133
HspAI GCGC 3 cut(s) 254, 1059, 1083
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 5 cut(s) 61, 101, 148, 183, 1040
LmnI GCTCC 3 cut(s) 778, 883, 1009
Lsp1109I GCAGC 5 cut(s) 387, 390, 431, 766, 1024
LweI GCATC 4 cut(s) 235, 431, 541, 879
MaeI CTAG 4 cut(s) 30, 90, 644, 755
MaeII ACGT 2 cut(s) 499, 508
MaeIII GTNAC 3 cut(s) 217, 347, 805
MalI GATC 5 cut(s) 63, 103, 150, 185, 1042
MboI GATC 5 cut(s) 61, 101, 148, 183, 1040
MboII GAAGA 5 cut(s) 575, 625, 817, 950, 1064
MfeI CAATTG 2 cut(s) 789, 1044
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 8 cut(s) 178, 312, 658, 789, 925, 969, 1044, 1064
MluI ACGCGT 1 cut(s) 10
MlyI GAGTC 2 cut(s) 158, 729
MreI CGCCGGCG 1 cut(s) 73
MroNI GCCGGC 1 cut(s) 73
MseI TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
MslI CAYNNNNRTG 2 cut(s) 278, 1119
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 1 cut(s) 74
MunI CAATTG 2 cut(s) 789, 1044
Mva1269I GAATGC 3 cut(s) 412, 472, 775
MvnI CGCG 3 cut(s) 12, 68, 242
MwoI GCNNNNNNNGC 6 cut(s) 74, 232, 441, 779, 785, 1082
NaeI GCCGGC 1 cut(s) 75
NdeII GATC 5 cut(s) 61, 101, 148, 183, 1040
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 2 cut(s) 422, 1133
NmuCI GTSAC 1 cut(s) 347
NspI RCATGY 1 cut(s) 422
PagI TCATGA 1 cut(s) 1129
PciI ACATGT 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PctI GAATGC 3 cut(s) 412, 472, 775
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 1 cut(s) 514
PkrI GCNGC 5 cut(s) 377, 380, 446, 781, 1014
PleI GAGTC 2 cut(s) 158, 728
PpsI GAGTC 2 cut(s) 158, 728
PscI ACATGT 1 cut(s) 418
PspPI GGNCC 1 cut(s) 458
PstNI CAGNNNCTG 2 cut(s) 578, 803
PsuI RGATCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 4 cut(s) 96, 291, 961, 1113
RsaNI GTAC 4 cut(s) 95, 290, 960, 1112
RseI CAYNNNNRTG 2 cut(s) 278, 1119
SaqAI TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
SatI GCNGC 5 cut(s) 376, 379, 445, 780, 1013
Sau3AI GATC 5 cut(s) 61, 101, 148, 183, 1040
Sau96I GGNCC 1 cut(s) 458
ScaI AGTACT 1 cut(s) 96
SchI GAGTC 2 cut(s) 158, 729
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 4 cut(s) 235, 431, 541, 879
SfcI CTRYAG 1 cut(s) 519
SgrAI CRCCGGYG 1 cut(s) 73
SmiMI CAYNNNNRTG 2 cut(s) 278, 1119
SmlI CTYRAG 2 cut(s) 945, 982
SmoI CTYRAG 2 cut(s) 945, 982
SpeI ACTAGT 1 cut(s) 29
Sse9I AATT 8 cut(s) 178, 312, 658, 789, 925, 969, 1044, 1064
SsiI CCGC 3 cut(s) 36, 77, 242
SspI AATATT 1 cut(s) 607
SspMI CTAG 4 cut(s) 30, 90, 644, 755
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 5 cut(s) 118, 338, 580, 676, 805
TaiI ACGT 2 cut(s) 502, 511
TaqI TCGA 3 cut(s) 182, 439, 723
TasI AATT 8 cut(s) 178, 312, 658, 789, 925, 969, 1044, 1064
TatI WGTACW 2 cut(s) 94, 1111
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
Tru9I TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
TscAI CASTG 3 cut(s) 121, 681, 808
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 5 cut(s) 375, 378, 444, 779, 1012
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 5 cut(s) 60, 153, 479, 872, 1146
TspRI CASTG 3 cut(s) 121, 681, 808
XapI RAATTY 2 cut(s) 658, 1064
XceI RCATGY 1 cut(s) 422
XspI CTAG 4 cut(s) 30, 90, 644, 755
ZrmI AGTACT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.