Rroxscaffold_1G00002910

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000940
Physical Location & Seq
Reverse (-)
3429032 .. 3431279
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002910.1

Sequence Viewer

Length: 882 bp
ATGGCTGAGAAAGAAGGTACTGTCAATATTGAGCGACTCGACTTCGGCCAAGACCCAGAGCCCCGCCGAAAGCTGCTTCGGCTACCTCGCCGTCCGAAGAAAAGAGACGATATAGTGAGTCCGGGATCAAAGAAATTTGATGTTGTTATTGACGAAATTAACAGACTTCATGAGCAAGTACAGCTTCCCCGAGAACAAGTGTCCGATGCGCAAGCACTTCTAGACGTAACTAAAACCGTGGTGGCCCCTATTATGTCACATTCTAATGGGGAAACTACCACAAGCGATTTCATTACCAGTTTGATTACAAATTTCGGGCAAGTCAATAGGTCATCAGTGAACGAAGATGATGCTCATGTCTCACTTAACTGGAAAGATATTGGACTTTCTGTTCCACCAACACTCAAGAGGGCTTTTGGATGTTGCACAATGCTTGGACCCATGAATACTGAGTATAAGGAAAGGAAGGCTGCTGTCCGGCGTACAAAGCCTACCACAGCTCATCTGCCGACAGAGATTAATGAAGGAGGGGAAACAAATGAGACAGACTTAAACATTTTAACAATGTATGAGATTTTAAAGAAGAAGAAGAATGTCGAACTCAGTTGTTTGATACTAAATAGAAGGTCTTTTGCACAGACCGTGGAGAATTTATTCGCTCTTTCATTCCTCGCAAAAGACGGCAGAGTTAGAATTGCCGTCGAAGCAAATGGTTCTCATGTCGTTTCACCTACGAATGGTCCTGTTGCCAATGCAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATAATGACTGAAATGGTGGCTGTTGGTGAGGAACTGATGCCTCATAGAGACCACACAAAGTGCACACCTGCCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

293

Amino Acids

32.96

Weight (kDa)

7.73

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 199 - 281 4.4e-20 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 210
AciI CCGC 1 cut(s) 64
AclWI GGATC 1 cut(s) 133
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 3 cut(s) 134, 310, 649
AdeI CACNNNGTG 1 cut(s) 864
AfaI GTAC 3 cut(s) 19, 180, 484
AfiI CCNNNNNNNGG 1 cut(s) 737
AgsI TTSAA 1 cut(s) 793
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 3 cut(s) 73, 184, 500
AluI AGCT 3 cut(s) 73, 184, 500
Alw21I GWGCWC 1 cut(s) 869
Alw26I GTCTC 4 cut(s) 99, 364, 536, 846
Alw44I GTGCAC 1 cut(s) 865
AlwI GGATC 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 2 cut(s) 46, 243
ApaLI GTGCAC 1 cut(s) 865
ApeKI GCWGC 2 cut(s) 73, 470
ApoI RAATTY 3 cut(s) 134, 310, 649
AseI ATTAAT 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 653
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 3 cut(s) 244, 437, 740
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 3 cut(s) 720, 758, 842
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 2 cut(s) 437, 740
BaeGI GKGCMC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 869
BbvI GCAGC 2 cut(s) 60, 457
BceAI ACGGC 3 cut(s) 75, 683, 697
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 4 cut(s) 99, 364, 536, 846
BfaI CTAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 74, 471
BlsI GCNGC 2 cut(s) 75, 472
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 437, 740
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 3 cut(s) 244, 437, 740
BmiI GGNNCC 2 cut(s) 246, 439
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 3 cut(s) 196, 340, 831
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 846
BsaJI CCNNGG 2 cut(s) 237, 642
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse1I ACTGG 3 cut(s) 297, 374, 803
BseDI CCNNGG 2 cut(s) 237, 642
BseGI GGATG 1 cut(s) 425
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMII CTCAG 2 cut(s) 441, 616
BseNI ACTGG 3 cut(s) 297, 374, 803
BseSI GKGCMC 1 cut(s) 869
BseXI GCAGC 2 cut(s) 60, 457
BshFI GGCC 2 cut(s) 48, 245
BsiHKAI GWGCWC 1 cut(s) 869
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 122, 478
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 4 cut(s) 99, 364, 536, 846
BsmBI CGTCTC 1 cut(s) 99
BsnI GGCC 2 cut(s) 48, 245
Bso31I GGTCTC 1 cut(s) 846
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 63, 869
Bsp143I GATC 1 cut(s) 125
BspACI CCGC 1 cut(s) 64
BspANI GGCC 2 cut(s) 48, 245
BspCNI CTCAG 2 cut(s) 442, 615
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 246, 439
BspPI GGATC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 846
BsrI ACTGG 3 cut(s) 297, 374, 803
BssECI CCNNGG 2 cut(s) 237, 642
BssMI GATC 1 cut(s) 125
Bst4CI ACNGT 3 cut(s) 22, 238, 643
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 4 cut(s) 6, 450, 602, 879
BstDSI CCRYGG 2 cut(s) 237, 642
BstF5I GGATG 1 cut(s) 425
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 1 cut(s) 128
BstMAI GTCTC 4 cut(s) 99, 364, 536, 846
BstMBI GATC 1 cut(s) 125
BstMWI GCNNNNNNNGC 4 cut(s) 79, 181, 487, 704
BstSCI CCNGG 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 869
BstV1I GCAGC 2 cut(s) 60, 457
BstXI CCANNNNNNTGG 1 cut(s) 757
BsuRI GGCC 2 cut(s) 48, 245
BtgI CCRYGG 2 cut(s) 237, 642
BtsCI GGATG 1 cut(s) 425
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 2 cut(s) 342, 762
Cac8I GCNNGC 1 cut(s) 213
CciI TCATGA 1 cut(s) 169
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 3 cut(s) 244, 437, 740
Csp6I GTAC 3 cut(s) 18, 179, 483
CviAII CATG 4 cut(s) 170, 356, 442, 719
CviQI GTAC 3 cut(s) 18, 179, 483
DdeI CTNAG 4 cut(s) 6, 450, 602, 879
DpnI GATC 1 cut(s) 127
DpnII GATC 1 cut(s) 125
DraI TTTAAA 1 cut(s) 579
DraIII CACNNNGTG 1 cut(s) 864
EaeI YGGCCR 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 846
Eco47I GGWCC 2 cut(s) 437, 740
Eco88I CYCGRG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 4 cut(s) 173, 359, 445, 722
FaiI YATR 9 cut(s) 113, 171, 254, 357, 443, 456, 570, 720, 849
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FatI CATG 4 cut(s) 169, 355, 441, 718
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 2 cut(s) 74, 471
FokI GGATG 1 cut(s) 432
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 2 cut(s) 74, 471
FspBI CTAG 1 cut(s) 221
FspI TGCGCA 1 cut(s) 210
GlaI GCGC 1 cut(s) 210
GluI GCNGC 2 cut(s) 74, 471
HaeIII GGCC 2 cut(s) 48, 245
HapII CCGG 2 cut(s) 122, 478
HhaI GCGC 1 cut(s) 211
Hin1II CATG 4 cut(s) 173, 359, 445, 722
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HinfI GANTC 2 cut(s) 36, 118
HpaII CCGG 2 cut(s) 122, 478
HphI GGTGA 3 cut(s) 720, 758, 842
Hpy166II GTNNAC 2 cut(s) 340, 867
Hpy188I TCNGA 3 cut(s) 96, 205, 782
Hpy188III TCNNGA 3 cut(s) 170, 221, 406
Hpy8I GTNNAC 2 cut(s) 340, 867
Hpy99I CGWCG 1 cut(s) 704
HpyAV CCTTC 4 cut(s) 8, 460, 518, 618
HpyCH4III ACNGT 3 cut(s) 22, 238, 643
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 4 cut(s) 426, 635, 755, 867
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 181, 487, 704
HpyF3I CTNAG 4 cut(s) 6, 450, 602, 879
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 4 cut(s) 173, 359, 445, 722
HspAI GCGC 1 cut(s) 209
Kzo9I GATC 1 cut(s) 125
LpnPI CCDG 7 cut(s) 69, 135, 310, 355, 491, 756, 784
Lsp1109I GCAGC 2 cut(s) 60, 457
LweI GCATC 3 cut(s) 196, 340, 831
MaeI CTAG 1 cut(s) 221
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 226, 255
MalI GATC 1 cut(s) 127
MboI GATC 1 cut(s) 125
MboII GAAGA 6 cut(s) 109, 356, 595, 598, 601, 814
MhlI GDGCHC 2 cut(s) 63, 869
MluCI AATT 5 cut(s) 134, 156, 310, 649, 693
MlyI GAGTC 2 cut(s) 30, 127
MnlI CCTC 6 cut(s) 96, 402, 521, 680, 826, 855
MroXI GAANNNNTTC 1 cut(s) 653
MseI TTAA 6 cut(s) 159, 366, 519, 551, 560, 578
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 2 cut(s) 122, 478
MspR9I CCNGG 1 cut(s) 123
MwoI GCNNNNNNNGC 4 cut(s) 79, 181, 487, 704
NciI CCSGG 1 cut(s) 123
NdeII GATC 1 cut(s) 125
NlaIII CATG 4 cut(s) 173, 359, 445, 722
NlaIV GGNNCC 2 cut(s) 246, 439
NmuCI GTSAC 1 cut(s) 255
NsbI TGCGCA 1 cut(s) 210
PagI TCATGA 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 653
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 2 cut(s) 75, 472
PleI GAGTC 2 cut(s) 30, 126
PpsI GAGTC 2 cut(s) 30, 126
PshBI ATTAAT 1 cut(s) 519
PspN4I GGNNCC 2 cut(s) 246, 439
PspPI GGNCC 3 cut(s) 244, 437, 740
RsaI GTAC 3 cut(s) 19, 180, 484
RsaNI GTAC 3 cut(s) 18, 179, 483
RseI CAYNNNNRTG 1 cut(s) 264
SaqAI TTAA 6 cut(s) 159, 366, 519, 551, 560, 578
SatI GCNGC 2 cut(s) 74, 471
Sau3AI GATC 1 cut(s) 125
Sau96I GGNCC 3 cut(s) 244, 437, 740
SchI GAGTC 2 cut(s) 30, 127
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 2 cut(s) 63, 869
SfaNI GCATC 3 cut(s) 196, 340, 831
SinI GGWCC 2 cut(s) 437, 740
SmiMI CAYNNNNRTG 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 5 cut(s) 134, 156, 310, 649, 693
SsiI CCGC 1 cut(s) 64
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 3 cut(s) 22, 238, 643
TaiI ACGT 1 cut(s) 228
TaqI TCGA 3 cut(s) 39, 597, 702
TasI AATT 5 cut(s) 134, 156, 310, 649, 693
TatI WGTACW 1 cut(s) 178
Tru1I TTAA 6 cut(s) 159, 366, 519, 551, 560, 578
Tru9I TTAA 6 cut(s) 159, 366, 519, 551, 560, 578
TscAI CASTG 2 cut(s) 342, 762
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 73, 470
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 5 cut(s) 158, 280, 458, 537, 654
TspRI CASTG 2 cut(s) 342, 762
VneI GTGCAC 1 cut(s) 865
VpaK11BI GGWCC 2 cut(s) 437, 740
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 3 cut(s) 134, 310, 649
XbaI TCTAGA 1 cut(s) 220
XmnI GAANNNNTTC 1 cut(s) 653
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.