Rh5AG523000

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000940
Physical Location & Seq
Forward (+)
89764812 .. 89767465
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG523000.1

Sequence Viewer

Length: 897 bp
ATGGCTGAGAAAGAAGGTACTGTCAATATTGAGCGACTCGACTTCGGCCAAGACCCGGAGCCCCGCCGAAAGCTCCGCTCCGGCTACCTCGCCGTCCGGAAGCTGACCAGTGAGAAAAGAGACGATATAGTGAGTCCGGAGTCAAAGAAATTTGATGTTGTTATTGACGAAATTAACAGACTTCATGAGCAAGTACAGCTTCCCAGAGAACAAGTCTCGGATGCGCAAGCACTTCTAGACGTAACTAAAACCGTGGTGGCCCCTATTATGTCACATCATAATGGGGAAACTACCACAAGCGATTTCATTACCAGTTTGATTTCAAATTTCGGGCAAGTCAATAGGTCATCAGTGAACGAAGATGATGCCCATGTCTCACTTAACTGGAAAGATATTGGACTTTCTGTTCCACCAACACTCAAGAGGGCTTTTGGATGTTGCACAATGCTTGGACCCATGGATACTGAGTATAAGGAAAGGAAGGCTGCTGTCCGCCGTTTAAAGCCTACCACAGCTCATCTGCCGACACAGATTAATGAAGGAGGGGAAACAAATGAGACAGACTTAAACATGTTAACAATGTATGAGATATTAAAGAAGAAGAAGAATGTCGAACTCAGTTGTTTGATACTGAATAGAAGGTCTTTTGCACAGACCGTGGAGAATCTATTCGCTCTTTCATTCCTCGCAAAAGACGGCAGAGTTAGAATTGCCGTCGAAGCAAGCGGTTCTCATGTCGTTTCACCTACGAATGGTCCTGTTGCCAATGCAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATAATGACTGAAATGGTGGCTGTTGGTGAGGAGCTGATGCCTCATAGAGACCACACAAATTGCACACCTGCCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

298

Amino Acids

33.5

Weight (kDa)

6.38

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 204 - 286 8.7e-20 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 78
AccIII TCCGGA 2 cut(s) 96, 136
AciI CCGC 4 cut(s) 64, 76, 493, 726
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 149, 325
AfaI GTAC 2 cut(s) 19, 195
AfiI CCNNNNNNNGG 2 cut(s) 55, 752
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 2 cut(s) 324, 808
AloI GAACNNNNNNTCC 2 cut(s) 390, 422
AluBI AGCT 5 cut(s) 73, 103, 199, 515, 853
AluI AGCT 5 cut(s) 73, 103, 199, 515, 853
Alw26I GTCTC 5 cut(s) 114, 220, 379, 551, 861
Aor13HI TCCGGA 2 cut(s) 96, 136
AoxI GGCC 2 cut(s) 46, 258
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 2 cut(s) 149, 325
AseI ATTAAT 1 cut(s) 534
Asp700I GAANNNNTTC 1 cut(s) 668
AspLEI GCGC 1 cut(s) 226
AspS9I GGNCC 3 cut(s) 259, 452, 755
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 735, 773, 857
AvaII GGWCC 2 cut(s) 452, 755
BanII GRGCYC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 472
BceAI ACGGC 4 cut(s) 77, 480, 698, 712
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciVI GTATCC 1 cut(s) 454
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 5 cut(s) 114, 220, 379, 551, 861
BfaI CTAG 1 cut(s) 236
BfuI GTATCC 1 cut(s) 454
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 2 cut(s) 452, 755
BmgT120I GGNCC 3 cut(s) 259, 452, 755
BmiI GGNNCC 3 cut(s) 60, 261, 454
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 3 cut(s) 211, 355, 846
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 861
BsaJI CCNNGG 3 cut(s) 252, 456, 657
BsaWI WCCGGW 2 cut(s) 96, 136
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 752
Bse1I ACTGG 4 cut(s) 108, 312, 389, 818
BseAI TCCGGA 2 cut(s) 96, 136
BseDI CCNNGG 3 cut(s) 252, 456, 657
BseGI GGATG 2 cut(s) 226, 440
BseLI CCNNNNNNNGG 2 cut(s) 55, 752
BseMII CTCAG 2 cut(s) 456, 631
BseNI ACTGG 4 cut(s) 108, 312, 389, 818
BseRI GAGGAG 1 cut(s) 863
BseXI GCAGC 1 cut(s) 472
BshFI GGCC 2 cut(s) 48, 260
BsiSI CCGG 4 cut(s) 56, 81, 97, 137
BslI CCNNNNNNNGG 2 cut(s) 55, 752
BsmAI GTCTC 5 cut(s) 114, 220, 379, 551, 861
BsmBI CGTCTC 1 cut(s) 114
BsnI GGCC 2 cut(s) 48, 260
Bso31I GGTCTC 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 63
Bsp13I TCCGGA 2 cut(s) 96, 136
Bsp19I CCATGG 1 cut(s) 456
BspACI CCGC 4 cut(s) 64, 76, 493, 726
BspANI GGCC 2 cut(s) 48, 260
BspCNI CTCAG 2 cut(s) 457, 630
BspEI TCCGGA 2 cut(s) 96, 136
BspHI TCATGA 1 cut(s) 184
BspLI GGNNCC 3 cut(s) 60, 261, 454
BspTNI GGTCTC 1 cut(s) 861
BsrBI CCGCTC 1 cut(s) 78
BsrI ACTGG 4 cut(s) 108, 312, 389, 818
BssECI CCNNGG 3 cut(s) 252, 456, 657
BssT1I CCWWGG 1 cut(s) 456
Bst4CI ACNGT 3 cut(s) 22, 253, 658
BstC8I GCNNGC 2 cut(s) 228, 724
BstDEI CTNAG 4 cut(s) 6, 465, 617, 894
BstDSI CCRYGG 3 cut(s) 252, 456, 657
BstF5I GGATG 2 cut(s) 226, 440
BstHHI GCGC 1 cut(s) 226
BstMAI GTCTC 5 cut(s) 114, 220, 379, 551, 861
BstMWI GCNNNNNNNGC 2 cut(s) 196, 719
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 472
BstXI CCANNNNNNTGG 1 cut(s) 772
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 2 cut(s) 48, 260
BtgI CCRYGG 3 cut(s) 252, 456, 657
BtsCI GGATG 2 cut(s) 226, 440
BtsI GCAGTG 1 cut(s) 777
BtsIMutI CAGTG 3 cut(s) 115, 357, 777
Cac8I GCNNGC 2 cut(s) 228, 724
CciI TCATGA 1 cut(s) 184
CfoI GCGC 1 cut(s) 226
Cfr13I GGNCC 3 cut(s) 259, 452, 755
Csp6I GTAC 2 cut(s) 18, 194
CspCI CAANNNNNGTGG 2 cut(s) 860, 895
CviAII CATG 5 cut(s) 185, 371, 457, 571, 734
CviQI GTAC 2 cut(s) 18, 194
DdeI CTNAG 4 cut(s) 6, 465, 617, 894
DraI TTTAAA 1 cut(s) 501
EaeI YGGCCR 1 cut(s) 46
EciI GGCGGA 1 cut(s) 482
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 63
Eco31I GGTCTC 1 cut(s) 861
Eco47I GGWCC 2 cut(s) 452, 755
EcoT14I CCWWGG 1 cut(s) 456
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 456
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 5 cut(s) 188, 374, 460, 574, 737
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 5 cut(s) 184, 370, 456, 570, 733
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 2 cut(s) 233, 447
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 1 cut(s) 236
FspI TGCGCA 1 cut(s) 225
GlaI GCGC 1 cut(s) 225
GluI GCNGC 1 cut(s) 486
HaeIII GGCC 2 cut(s) 48, 260
HapII CCGG 4 cut(s) 56, 81, 97, 137
HhaI GCGC 1 cut(s) 226
Hin1II CATG 5 cut(s) 188, 374, 460, 574, 737
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HinfI GANTC 4 cut(s) 36, 133, 140, 664
HpaI GTTAAC 1 cut(s) 576
HpaII CCGG 4 cut(s) 56, 81, 97, 137
HphI GGTGA 3 cut(s) 735, 773, 857
Hpy166II GTNNAC 2 cut(s) 355, 576
Hpy188I TCNGA 2 cut(s) 220, 797
Hpy188III TCNNGA 5 cut(s) 97, 137, 185, 236, 421
Hpy8I GTNNAC 2 cut(s) 355, 576
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 4 cut(s) 8, 475, 533, 633
HpyCH4III ACNGT 3 cut(s) 22, 253, 658
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 4 cut(s) 441, 650, 770, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 719
HpyF3I CTNAG 4 cut(s) 6, 465, 617, 894
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 5 cut(s) 188, 374, 460, 574, 737
HspAI GCGC 1 cut(s) 224
Kpn2I TCCGGA 2 cut(s) 96, 136
KspAI GTTAAC 1 cut(s) 576
LmnI GCTCC 4 cut(s) 58, 78, 83, 850
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 3 cut(s) 211, 355, 846
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 2 cut(s) 241, 270
MbiI CCGCTC 1 cut(s) 78
MboII GAAGA 5 cut(s) 371, 610, 613, 616, 829
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 5 cut(s) 149, 171, 325, 708, 877
MlyI GAGTC 3 cut(s) 30, 142, 149
MnlI CCTC 6 cut(s) 98, 417, 536, 695, 841, 870
MroI TCCGGA 2 cut(s) 96, 136
MroXI GAANNNNTTC 1 cut(s) 668
MseI TTAA 7 cut(s) 174, 381, 500, 534, 566, 575, 593
MslI CAYNNNNRTG 1 cut(s) 279
MspI CCGG 4 cut(s) 56, 81, 97, 137
MspR9I CCNGG 1 cut(s) 56
MwoI GCNNNNNNNGC 2 cut(s) 196, 719
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 456
NlaIII CATG 5 cut(s) 188, 374, 460, 574, 737
NlaIV GGNNCC 3 cut(s) 60, 261, 454
NmuCI GTSAC 1 cut(s) 270
NsbI TGCGCA 1 cut(s) 225
NspI RCATGY 1 cut(s) 574
PagI TCATGA 1 cut(s) 184
PciI ACATGT 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 668
PfeI GAWTC 1 cut(s) 664
PkrI GCNGC 1 cut(s) 487
PleI GAGTC 3 cut(s) 30, 141, 148
PpsI GAGTC 3 cut(s) 30, 141, 148
PscI ACATGT 1 cut(s) 570
PshBI ATTAAT 1 cut(s) 534
PspN4I GGNNCC 3 cut(s) 60, 261, 454
PspPI GGNCC 3 cut(s) 259, 452, 755
RsaI GTAC 2 cut(s) 19, 195
RsaNI GTAC 2 cut(s) 18, 194
RseI CAYNNNNRTG 1 cut(s) 279
SaqAI TTAA 7 cut(s) 174, 381, 500, 534, 566, 575, 593
SatI GCNGC 1 cut(s) 486
Sau96I GGNCC 3 cut(s) 259, 452, 755
SchI GAGTC 3 cut(s) 30, 142, 149
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 1 cut(s) 63
SfaNI GCATC 3 cut(s) 211, 355, 846
SinI GGWCC 2 cut(s) 452, 755
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 5 cut(s) 149, 171, 325, 708, 877
SsiI CCGC 4 cut(s) 64, 76, 493, 726
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 3 cut(s) 22, 253, 658
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 39, 612, 717
TasI AATT 5 cut(s) 149, 171, 325, 708, 877
TatI WGTACW 1 cut(s) 193
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 7 cut(s) 174, 381, 500, 534, 566, 575, 593
Tru9I TTAA 7 cut(s) 174, 381, 500, 534, 566, 575, 593
TscAI CASTG 3 cut(s) 115, 357, 777
TseFI GTSAC 1 cut(s) 270
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 4 cut(s) 173, 295, 552, 669
TspRI CASTG 3 cut(s) 115, 357, 777
VpaK11BI GGWCC 2 cut(s) 452, 755
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 2 cut(s) 149, 325
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 668
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.