Rw0G023200

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01079
OG0000940
Physical Location & Seq
Reverse (-)
39311 .. 39944
634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023200.1

Sequence Viewer

Length: 369 bp
ATGGATACTGAGTATAAGGAAAGGAAGGCTGCTGTCCGCCGTACAAAGCCTACCACAGCTCGTCTGCCGACACAGATTAATGAAGGAGGGGAAACAAATGAGACAGACTTAAACATGTTAACAATGTATGAGATATTAAAGAAGAAGAAGAATGTCGAACTCAGTTGTTTGATACTAAATAGAAGGTCTTTTGCACAGACCGTGGAGAATCTATTCGCTCTTTCATTCCTCGCAAAAGACGGCAGAGTCAGAATTGCCGTCGAAGCAAATGGTTTTCATGTCGTTTCACCTACGAATGGTCCTGTTGCCAATGCAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

122

Amino Acids

13.98

Weight (kDa)

9.52

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 52 - 121 1.1e-18 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AciI CCGC 1 cut(s) 37
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 296
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 1 cut(s) 352
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 29
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 279, 317
AvaII GGWCC 1 cut(s) 299
BbvI GCAGC 1 cut(s) 16
BceAI ACGGC 3 cut(s) 24, 242, 256
BcoDI GTCTC 1 cut(s) 95
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 362
BseDI CCNNGG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 362
BseXI GCAGC 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 95
BspACI CCGC 1 cut(s) 37
BspCNI CTCAG 1 cut(s) 174
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 201
Bst4CI ACNGT 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 9, 161
BstDSI CCRYGG 1 cut(s) 201
BstMAI GTCTC 1 cut(s) 95
BstMWI GCNNNNNNNGC 1 cut(s) 263
BstNSI RCATGY 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 16
BstXI CCANNNNNNTGG 1 cut(s) 316
BtgI CCRYGG 1 cut(s) 201
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
Cfr13I GGNCC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 42
CviAII CATG 2 cut(s) 115, 278
CviJI RGCY 4 cut(s) 29, 49, 59, 320
CviKI_1 RGCY 4 cut(s) 29, 49, 59, 320
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 2 cut(s) 9, 161
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EciI GGCGGA 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 299
FaeI CATG 2 cut(s) 118, 281
FaiI YATR 5 cut(s) 15, 116, 129, 279, 367
FatI CATG 2 cut(s) 114, 277
Fnu4HI GCNGC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
Hin1II CATG 2 cut(s) 118, 281
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HinfI GANTC 2 cut(s) 208, 246
HpaI GTTAAC 1 cut(s) 120
HphI GGTGA 2 cut(s) 279, 317
Hpy166II GTNNAC 1 cut(s) 120
Hpy188I TCNGA 2 cut(s) 251, 341
Hpy8I GTNNAC 1 cut(s) 120
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 4 cut(s) 19, 77, 177, 355
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 194, 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 263
HpyF3I CTNAG 2 cut(s) 9, 161
Hsp92II CATG 2 cut(s) 118, 281
KspAI GTTAAC 1 cut(s) 120
LpnPI CCDG 2 cut(s) 315, 343
Lsp1109I GCAGC 1 cut(s) 16
MboII GAAGA 3 cut(s) 154, 157, 160
MluCI AATT 1 cut(s) 252
MlyI GAGTC 1 cut(s) 255
MnlI CCTC 2 cut(s) 80, 239
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 4 cut(s) 78, 110, 119, 137
MwoI GCNNNNNNNGC 1 cut(s) 263
NlaIII CATG 2 cut(s) 118, 281
NspI RCATGY 1 cut(s) 118
PciI ACATGT 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PscI ACATGT 1 cut(s) 114
PshBI ATTAAT 1 cut(s) 78
PspPI GGNCC 1 cut(s) 299
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
SaqAI TTAA 4 cut(s) 78, 110, 119, 137
SatI GCNGC 1 cut(s) 30
Sau96I GGNCC 1 cut(s) 299
SchI GAGTC 1 cut(s) 255
SetI ASST 4 cut(s) 61, 188, 292, 366
SgeI CNNG 7 cut(s) 72, 127, 214, 242, 290, 314, 364
SinI GGWCC 1 cut(s) 299
Sse9I AATT 1 cut(s) 252
SsiI CCGC 1 cut(s) 37
TaaI ACNGT 1 cut(s) 202
TaqI TCGA 2 cut(s) 156, 261
TasI AATT 1 cut(s) 252
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 4 cut(s) 78, 110, 119, 137
Tru9I TTAA 4 cut(s) 78, 110, 119, 137
TscAI CASTG 1 cut(s) 321
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 96, 213, 266
TspRI CASTG 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 299
VspI ATTAAT 1 cut(s) 78
XceI RCATGY 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.