Rmu_sc0020711.1_g000002

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020711.1
OG0000940
Physical Location & Seq
Forward (+)
7240 .. 9540
2301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020711.1_g000002.1.cds

Sequence Viewer

Length: 1158 bp
atgccgaggaccgcgaaacgcgaccggagcgcctgtcaaggccgtggcgtcgatgacggcgaggaggcggagcggctccgagccgtgaagcgcgagcgactcaattctgagcaggttgaaaatgagtcaaccgatcaggacgccggtcaacggcgagctctccgatctggctacaatgaaatcctgaacctcattagtgagaagagaaatgagttattgaagccggagtcggagaaatttggcgtcatcattggtagagttgagaagttgcatgagctagttcagaagccaagagagcaagtggcagatgcagaggcactattggacataactaacacattggtaacctctgtgaagtcacagtccagtgccggcattactccatcagacttcattacctgtttgattcgagattttggacaattaagggatggccatgcctcgattcggtggaaagacgttggggtggccgtggcaccaattttcaagagggctcatgggtgctgtacaatgcttggacccatgaatactgagtataaggaaaggaaggcagctgttcgtactagacgaacaaagcctaccacaactgatgagcctgaggagattgatgcacaggaagaagaaaaaactgatacggataggaatatgtcaacgatgtttgagatattaagaaagaagaaacgtgtccgactggaatgtttaatattaaacagaatgttctttgcacagacagtcgagaatttatttgctctttcattccttgttaaagatggccgggctgaaatcatagttgatgcaaatcgcgttcattttgttgcaccaaggaatgctgccaaggccactgaagtggcatctcgtgaggttgtttatagacactttgtgttccgatttgatttcaaggactggaagttgatgaaggatatggtgcctgatggtgaggagctaatgccacatagaatccctgccaactctgaacctgcctctcaggaggaaccagctgcatacgtgtcccaaacaggacacacaactccaattcgaaagctttccaggaatcgcggcaggatggtgcaggaagaagctattgtagaagaatcccctgaatatgataatgaccgtgctgctggaggcgcactttgcaggtctaagcgcaagctatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

385

Amino Acids

43.83

Weight (kDa)

8.43

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 103, 985, 1128
AccB1I GGYRCC 2 cut(s) 475, 925
AccBSI CCGCTC 1 cut(s) 73
AccII CGCG 5 cut(s) 14, 21, 93, 804, 1056
AciI CCGC 4 cut(s) 12, 68, 73, 1056
AcoI YGGCCR 3 cut(s) 433, 468, 772
AcsI RAATTY 2 cut(s) 236, 739
AcuI CTGAAG 1 cut(s) 864
AcyI GRCGYC 3 cut(s) 48, 141, 243
AdeI CACNNNGTG 1 cut(s) 880
AfaI GTAC 2 cut(s) 508, 562
AfiI CCNNNNNNNGG 2 cut(s) 150, 447
AflIII ACRYGT 2 cut(s) 682, 1005
AgsI TTSAA 4 cut(s) 119, 220, 487, 898
AjnI CCWGG 1 cut(s) 1046
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 8 cut(s) 158, 277, 554, 943, 998, 1042, 1079, 1153
AluI AGCT 8 cut(s) 158, 277, 554, 943, 998, 1042, 1079, 1153
Alw21I GWGCWC 1 cut(s) 160
AoxI GGCC 5 cut(s) 40, 433, 468, 772, 837
ApeKI GCWGC 4 cut(s) 551, 830, 998, 1118
ApoI RAATTY 2 cut(s) 236, 739
Asp700I GAANNNNTTC 1 cut(s) 1042
AspLEI GCGC 4 cut(s) 32, 93, 1130, 1149
AspS9I GGNCC 2 cut(s) 9, 518
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 1 cut(s) 947
AsuII TTCGAA 1 cut(s) 1036
AvaII GGWCC 2 cut(s) 9, 518
AxyI CCTNAGG 1 cut(s) 597
BalI TGGCCA 1 cut(s) 435
BanI GGYRCC 2 cut(s) 475, 925
BanII GRGCYC 2 cut(s) 160, 496
BauI CACGAG 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 4 cut(s) 563, 817, 985, 1105
BccI CCATC 5 cut(s) 391, 425, 764, 926, 1057
BceAI ACGGC 5 cut(s) 27, 68, 73, 167, 455
BciT130I CCWGG 1 cut(s) 1048
BcnI CCSGG 1 cut(s) 776
BfaI CTAG 2 cut(s) 278, 564
BfmI CTRYAG 1 cut(s) 1154
BfoI RGCGCY 1 cut(s) 33
BfuAI ACCTGC 3 cut(s) 103, 985, 1128
BisI GCNGC 6 cut(s) 74, 552, 831, 999, 1057, 1119
BlsI GCNGC 6 cut(s) 75, 553, 832, 1000, 1058, 1120
Bme1390I CCNGG 2 cut(s) 776, 1048
Bme18I GGWCC 2 cut(s) 9, 518
BmgT120I GGNCC 2 cut(s) 9, 518
BmiI GGNNCC 5 cut(s) 77, 477, 520, 927, 993
BmrFI CCNGG 2 cut(s) 776, 1048
BmsI GCATC 4 cut(s) 298, 598, 784, 860
BoxI GACNNNNGTC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 1143
Bpu14I TTCGAA 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 776
BsaAI YACGTR 1 cut(s) 1006
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 3 cut(s) 48, 141, 243
BsaJI CCNNGG 5 cut(s) 5, 43, 471, 821, 834
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 447
Bse118I RCCGGY 2 cut(s) 143, 371
Bse1I ACTGG 3 cut(s) 366, 696, 908
Bse21I CCTNAGG 1 cut(s) 597
Bse8I GATNNNNATC 1 cut(s) 798
BseBI CCWGG 1 cut(s) 1048
BseDI CCNNGG 5 cut(s) 5, 43, 471, 821, 834
BseGI GGATG 2 cut(s) 436, 1068
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 2 cut(s) 150, 447
BseMII CTCAG 4 cut(s) 99, 522, 588, 998
BseNI ACTGG 3 cut(s) 366, 696, 908
BseRI GAGGAG 3 cut(s) 77, 614, 953
BseXI GCAGC 4 cut(s) 563, 817, 985, 1105
BsgI GTGCAG 1 cut(s) 1088
Bsh1236I CGCG 5 cut(s) 14, 21, 93, 804, 1056
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 42, 435, 470, 774, 839
BshNI GGYRCC 2 cut(s) 475, 925
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 5 cut(s) 25, 144, 224, 372, 775
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 2 cut(s) 150, 447
BsmFI GGGAC 1 cut(s) 994
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 5 cut(s) 42, 435, 470, 774, 839
Bsp119I TTCGAA 1 cut(s) 1036
Bsp1286I GDGCHC 2 cut(s) 160, 496
Bsp1407I TGTACA 1 cut(s) 506
Bsp143I GATC 2 cut(s) 133, 164
BspACI CCGC 4 cut(s) 12, 68, 73, 1056
BspANI GGCC 5 cut(s) 42, 435, 470, 774, 839
BspCNI CTCAG 4 cut(s) 100, 523, 589, 997
BspFNI CGCG 5 cut(s) 14, 21, 93, 804, 1056
BspLI GGNNCC 5 cut(s) 77, 477, 520, 927, 993
BspMI ACCTGC 3 cut(s) 103, 985, 1128
BspT104I TTCGAA 1 cut(s) 1036
BspT107I GGYRCC 2 cut(s) 475, 925
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 2 cut(s) 143, 371
BsrGI TGTACA 1 cut(s) 506
BsrI ACTGG 3 cut(s) 366, 696, 908
BssAI RCCGGY 2 cut(s) 143, 371
BssECI CCNNGG 5 cut(s) 5, 43, 471, 821, 834
BssMI GATC 2 cut(s) 133, 164
BssNI GRCGYC 3 cut(s) 48, 141, 243
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 2 cut(s) 821, 834
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 1 cut(s) 1048
Bst4CI ACNGT 3 cut(s) 363, 733, 1115
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 3 cut(s) 48, 141, 243
BstAUI TGTACA 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 1006
BstBI TTCGAA 1 cut(s) 1036
BstC8I GCNNGC 4 cut(s) 95, 156, 373, 1151
BstDEI CTNAG 5 cut(s) 108, 531, 597, 984, 1143
BstDSI CCRYGG 2 cut(s) 43, 471
BstEII GGTNACC 1 cut(s) 343
BstF5I GGATG 2 cut(s) 436, 1068
BstFNI CGCG 5 cut(s) 14, 21, 93, 804, 1056
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 4 cut(s) 32, 93, 1130, 1149
BstKTI GATC 2 cut(s) 136, 167
BstMBI GATC 2 cut(s) 133, 164
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 5 cut(s) 27, 295, 836, 1127, 1134
BstNI CCWGG 1 cut(s) 1048
BstPAI GACNNNNGTC 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 343
BstSCI CCNGG 2 cut(s) 774, 1046
BstSFI CTRYAG 1 cut(s) 1154
BstUI CGCG 5 cut(s) 14, 21, 93, 804, 1056
BstV1I GCAGC 4 cut(s) 563, 817, 985, 1105
BstXI CCANNNNNNTGG 1 cut(s) 847
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 5 cut(s) 42, 435, 470, 774, 839
BtgI CCRYGG 2 cut(s) 43, 471
BtsCI GGATG 2 cut(s) 436, 1068
BtsIMutI CAGTG 2 cut(s) 373, 840
BveI ACCTGC 3 cut(s) 103, 985, 1128
Cac8I GCNNGC 4 cut(s) 95, 156, 373, 1151
CfoI GCGC 4 cut(s) 32, 93, 1130, 1149
Cfr10I RCCGGY 2 cut(s) 143, 371
Cfr13I GGNCC 2 cut(s) 9, 518
CseI GACGC 3 cut(s) 37, 149, 232
Csp6I GTAC 2 cut(s) 507, 561
CviAII CATG 4 cut(s) 272, 437, 497, 523
CviQI GTAC 2 cut(s) 507, 561
DdeI CTNAG 5 cut(s) 108, 531, 597, 984, 1143
DpnI GATC 2 cut(s) 135, 166
DpnII GATC 2 cut(s) 133, 164
DraIII CACNNNGTG 1 cut(s) 880
EaeI YGGCCR 3 cut(s) 433, 468, 772
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 821, 834
Eco24I GRGCYC 2 cut(s) 160, 496
Eco47I GGWCC 2 cut(s) 9, 518
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 864
Eco81I CCTNAGG 1 cut(s) 597
Eco91I GGTNACC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 1046
EcoT14I CCWWGG 2 cut(s) 821, 834
EcoT38I GRGCYC 2 cut(s) 160, 496
ErhI CCWWGG 2 cut(s) 821, 834
FaeI CATG 4 cut(s) 275, 440, 500, 526
FaqI GGGAC 1 cut(s) 994
FatI CATG 4 cut(s) 271, 436, 496, 522
Fnu4HI GCNGC 6 cut(s) 74, 552, 831, 999, 1057, 1119
FokI GGATG 2 cut(s) 443, 1075
FriOI GRGCYC 2 cut(s) 160, 496
Fsp4HI GCNGC 6 cut(s) 74, 552, 831, 999, 1057, 1119
FspBI CTAG 2 cut(s) 278, 564
GlaI GCGC 4 cut(s) 31, 92, 1129, 1148
GluI GCNGC 6 cut(s) 74, 552, 831, 999, 1057, 1119
GsuI CTGGAG 1 cut(s) 1143
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 5 cut(s) 42, 435, 470, 774, 839
HapII CCGG 5 cut(s) 25, 144, 224, 372, 775
HgaI GACGC 3 cut(s) 37, 149, 232
HhaI GCGC 4 cut(s) 32, 93, 1130, 1149
Hin1I GRCGYC 3 cut(s) 48, 141, 243
Hin1II CATG 4 cut(s) 275, 440, 500, 526
Hin6I GCGC 4 cut(s) 30, 91, 1128, 1147
HinP1I GCGC 4 cut(s) 30, 91, 1128, 1147
HincII GTYRAC 3 cut(s) 129, 149, 651
HindII GTYRAC 3 cut(s) 129, 149, 651
HindIII AAGCTT 1 cut(s) 1040
HinfI GANTC 8 cut(s) 99, 125, 227, 406, 445, 957, 1051, 1091
HpaII CCGG 5 cut(s) 25, 144, 224, 372, 775
HphI GGTGA 1 cut(s) 947
Hpy166II GTNNAC 3 cut(s) 129, 149, 651
Hpy188I TCNGA 9 cut(s) 80, 109, 164, 232, 285, 388, 689, 887, 973
Hpy188III TCNNGA 7 cut(s) 137, 184, 410, 487, 736, 857, 986
Hpy8I GTNNAC 3 cut(s) 129, 149, 651
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 541, 910
HpyCH4III ACNGT 3 cut(s) 363, 733, 1115
HpyCH4IV ACGT 3 cut(s) 459, 682, 1005
HpyCH4V TGCA 9 cut(s) 271, 311, 611, 725, 797, 818, 1001, 1069, 1137
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 295, 836, 1127, 1134
HpyF3I CTNAG 5 cut(s) 108, 531, 597, 984, 1143
HpySE526I ACGT 3 cut(s) 459, 682, 1005
Hsp92I GRCGYC 3 cut(s) 48, 141, 243
Hsp92II CATG 4 cut(s) 275, 440, 500, 526
HspAI GCGC 4 cut(s) 30, 91, 1128, 1147
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
Kzo9I GATC 2 cut(s) 133, 164
LmnI GCTCC 4 cut(s) 27, 70, 81, 940
Lsp1109I GCAGC 4 cut(s) 563, 817, 985, 1105
LweI GCATC 4 cut(s) 298, 598, 784, 860
MaeI CTAG 2 cut(s) 278, 564
MaeII ACGT 3 cut(s) 459, 682, 1005
MaeIII GTNAC 2 cut(s) 343, 357
MalI GATC 2 cut(s) 135, 166
MbiI CCGCTC 1 cut(s) 73
MboI GATC 2 cut(s) 133, 164
MboII GAAGA 6 cut(s) 214, 629, 632, 688, 1085, 1100
MhlI GDGCHC 2 cut(s) 160, 496
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 6 cut(s) 103, 236, 422, 480, 739, 1032
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 3 cut(s) 93, 134, 236
MmeI TCCRAC 2 cut(s) 210, 712
Mox20I TGGCCA 1 cut(s) 435
MroNI GCCGGC 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 1042
MscI TGGCCA 1 cut(s) 435
MseI TTAA 5 cut(s) 425, 668, 701, 707, 765
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 554, 998
MspI CCGG 5 cut(s) 25, 144, 224, 372, 775
MspR9I CCNGG 2 cut(s) 776, 1048
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 1 cut(s) 1048
MvnI CGCG 5 cut(s) 14, 21, 93, 804, 1056
MwoI GCNNNNNNNGC 5 cut(s) 27, 295, 836, 1127, 1134
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 1 cut(s) 776
NdeII GATC 2 cut(s) 133, 164
NgoMIV GCCGGC 1 cut(s) 371
NlaIII CATG 4 cut(s) 275, 440, 500, 526
NlaIV GGNNCC 5 cut(s) 77, 477, 520, 927, 993
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 357
NspV TTCGAA 1 cut(s) 1036
OliI CACNNNNGTG 1 cut(s) 845
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 565
PctI GAATGC 1 cut(s) 832
PdiI GCCGGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 1042
PfeI GAWTC 5 cut(s) 406, 445, 957, 1051, 1091
PfoI TCCNGGA 1 cut(s) 1046
PkrI GCNGC 6 cut(s) 75, 553, 832, 1000, 1058, 1120
PleI GAGTC 3 cut(s) 93, 133, 235
PpsI GAGTC 3 cut(s) 93, 133, 235
Ppu21I YACGTR 1 cut(s) 1006
PshAI GACNNNNGTC 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 1046
PspEI GGTNACC 1 cut(s) 343
PspGI CCWGG 1 cut(s) 1046
PspN4I GGNNCC 5 cut(s) 77, 477, 520, 927, 993
PspPI GGNCC 2 cut(s) 9, 518
PsrI GAACNNNNNNTAC 2 cut(s) 562, 594
PvuII CAGCTG 2 cut(s) 554, 998
RsaI GTAC 2 cut(s) 508, 562
RsaNI GTAC 2 cut(s) 507, 561
RseI CAYNNNNRTG 1 cut(s) 845
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 5 cut(s) 425, 668, 701, 707, 765
SatI GCNGC 6 cut(s) 74, 552, 831, 999, 1057, 1119
Sau3AI GATC 2 cut(s) 133, 164
Sau96I GGNCC 2 cut(s) 9, 518
SchI GAGTC 3 cut(s) 93, 134, 236
ScrFI CCNGG 2 cut(s) 776, 1048
SduI GDGCHC 2 cut(s) 160, 496
SfaNI GCATC 4 cut(s) 298, 598, 784, 860
SfcI CTRYAG 1 cut(s) 1154
SfuI TTCGAA 1 cut(s) 1036
SinI GGWCC 2 cut(s) 9, 518
SmiMI CAYNNNNRTG 1 cut(s) 845
Sse9I AATT 6 cut(s) 103, 236, 422, 480, 739, 1032
SsiI CCGC 4 cut(s) 12, 68, 73, 1056
SspI AATATT 1 cut(s) 705
SspMI CTAG 2 cut(s) 278, 564
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 2 cut(s) 774, 1046
StyI CCWWGG 2 cut(s) 821, 834
TaaI ACNGT 3 cut(s) 363, 733, 1115
TaiI ACGT 3 cut(s) 462, 685, 1008
TaqI TCGA 5 cut(s) 51, 409, 443, 735, 1036
TasI AATT 6 cut(s) 103, 236, 422, 480, 739, 1032
TatI WGTACW 1 cut(s) 506
TauI GCSGC 2 cut(s) 76, 1059
TfiI GAWTC 5 cut(s) 406, 445, 957, 1051, 1091
Tru1I TTAA 5 cut(s) 425, 668, 701, 707, 765
Tru9I TTAA 5 cut(s) 425, 668, 701, 707, 765
TscAI CASTG 2 cut(s) 373, 847
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 4 cut(s) 551, 830, 998, 1118
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 6 cut(s) 192, 382, 539, 744, 797, 929
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 2 cut(s) 373, 847
VpaK11BI GGWCC 2 cut(s) 9, 518
XapI RAATTY 2 cut(s) 236, 739
XmnI GAANNNNTTC 1 cut(s) 1042
XspI CTAG 2 cut(s) 278, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.