RLG00000036859

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000940
Physical Location & Seq
Forward (+)
85910283 .. 85912608
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036859

Sequence Viewer

Length: 1074 bp
ATGCCGAGGACCGTGAAACGTGACCGGAGCGCCGGTCAAGGCCGTGGCGTCGAAGACGGCGAGGAGGCGGAGAGGCTTAGAACCGTGAAGCGCGAGCGACTCAATTCCGAGCAGGTTGAAAATGAGTCAACCGATCAAGACGCCGGTCAACGGCGAGCTCTCCGATCTGACTACAATGAAATCCTGAACCTCATTAGTGAGAAGAGAAATGAGTTATTGAAGCCGGAATCGGAGAAGTTTGGCGTCATCATTGGTAGAGTTGAGAAGTTGCATGAGCTAGTTCAAAAGCCAAGAGAGCAAGTGGCAGATGCAGAGGCACTGTTAGACATAACCAACACATTAGTGACATCTGTTAAGTCACAGTCCAGTGCTGGCATTACCCCATCGGACTTCATTACCTGTTTGATTCGAGATTTTGGGCAATTAAGGGATGGCCATGCCTCGATTCGATGGAAAGATGTTGGGGTTGCTGTGGCGCCAATTTTCAAGAGGGCTCATGGGTGCTGCACAATGCTTGGACCCATGAATACTGAGTATAAGGAAAGGAAGGCAGCTGTTCGTACTAGACGAATAAAGCCTACCACAACTGATGAGCCTGAGGAGATTGATGCACAGGAAGAAGAAAAAACTGATACGGATAGGAATATGTCAACGATGTTTGAGATATTAAGAAAGAAGAAACATGTCCGACTGGAATGTTTAATATTAAACAGAATGTTCTTTGCACAGACAGTTGAGAATTTGTTTGCTCTTTCATTCCTTGTTAAAGACGGCCGGGCTGAAATCATAGTTGATGCAAATCGTGCTCATTTTGTTGGTATCTATTTGATGAAGGATATGGTGCCTGACGGTGAGGAGCTAATGCCACATAGAATCCCTGCCAACTCCGAACCTGCCTCTCAGGAGGAACCAGCTGCATACGTGTCCCAAACAGGACACACAACTCCAATTCGAAAGCTTTCCAGGAATCGCGGCCGGATGGTGCAGGAAGAAGCTATTGTAGAAGAATCCCCTGAATATGATAATGATCGTGCTGCTGGAGGCACACTTCGCAGGTCTAAGCGCAAGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

358

Amino Acids

40.57

Weight (kDa)

6.71

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 229 - 272 2.3e-11 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 103, 901, 1044
AccB1I GGYRCC 2 cut(s) 475, 841
AccII CGCG 2 cut(s) 93, 972
AciI CCGC 2 cut(s) 68, 972
AcoI YGGCCR 3 cut(s) 433, 772, 973
AcsI RAATTY 1 cut(s) 739
AcyI GRCGYC 4 cut(s) 48, 141, 243, 476
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 150
AflIII ACRYGT 2 cut(s) 682, 921
AgsI TTSAA 4 cut(s) 119, 220, 284, 487
AjnI CCWGG 1 cut(s) 962
AleI CACNNNNGTG 1 cut(s) 341
AluBI AGCT 8 cut(s) 158, 277, 554, 859, 914, 958, 995, 1069
AluI AGCT 8 cut(s) 158, 277, 554, 859, 914, 958, 995, 1069
Alw21I GWGCWC 2 cut(s) 160, 808
AoxI GGCC 4 cut(s) 40, 433, 772, 973
ApeKI GCWGC 4 cut(s) 504, 551, 914, 1034
ApoI RAATTY 1 cut(s) 739
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 4 cut(s) 32, 93, 478, 1065
AspS9I GGNCC 2 cut(s) 9, 518
AsuC2I CCSGG 1 cut(s) 776
AsuHPI GGTGA 1 cut(s) 863
AsuII TTCGAA 1 cut(s) 952
AvaII GGWCC 2 cut(s) 9, 518
AxyI CCTNAGG 1 cut(s) 597
BalI TGGCCA 1 cut(s) 435
BanI GGYRCC 2 cut(s) 475, 841
BanII GRGCYC 2 cut(s) 160, 496
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 160, 808
BbvI GCAGC 4 cut(s) 491, 563, 901, 1021
BccI CCATC 4 cut(s) 391, 425, 444, 973
BceAI ACGGC 4 cut(s) 27, 73, 167, 787
BciT130I CCWGG 1 cut(s) 964
BcnI CCSGG 1 cut(s) 776
BfaI CTAG 2 cut(s) 278, 564
BfmI CTRYAG 1 cut(s) 1070
BfoI RGCGCY 2 cut(s) 33, 479
BfuAI ACCTGC 3 cut(s) 103, 901, 1044
BisI GCNGC 5 cut(s) 505, 552, 915, 973, 1035
BlsI GCNGC 5 cut(s) 506, 553, 916, 974, 1036
Bme1390I CCNGG 2 cut(s) 776, 964
Bme18I GGWCC 2 cut(s) 9, 518
BmgT120I GGNCC 2 cut(s) 9, 518
BmiI GGNNCC 4 cut(s) 477, 520, 843, 909
BmrFI CCNGG 2 cut(s) 776, 964
BmsI GCATC 3 cut(s) 298, 598, 784
BoxI GACNNNNGTC 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 952
BpuMI CCSGG 1 cut(s) 776
BsaAI YACGTR 1 cut(s) 922
BsaBI GATNNNNATC 2 cut(s) 798, 1026
BsaHI GRCGYC 4 cut(s) 48, 141, 243, 476
BsaJI CCNNGG 2 cut(s) 5, 43
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 2 cut(s) 32, 143
Bse1I ACTGG 2 cut(s) 366, 696
Bse21I CCTNAGG 1 cut(s) 597
Bse8I GATNNNNATC 2 cut(s) 798, 1026
BseBI CCWGG 1 cut(s) 964
BseDI CCNNGG 2 cut(s) 5, 43
BseGI GGATG 2 cut(s) 436, 984
BseJI GATNNNNATC 2 cut(s) 798, 1026
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMII CTCAG 3 cut(s) 522, 588, 914
BseNI ACTGG 2 cut(s) 366, 696
BseRI GAGGAG 3 cut(s) 77, 614, 869
BseX3I CGGCCG 2 cut(s) 772, 973
BseXI GCAGC 4 cut(s) 491, 563, 901, 1021
BsgI GTGCAG 2 cut(s) 490, 1004
Bsh1236I CGCG 2 cut(s) 93, 972
Bsh1285I CGRYCG 2 cut(s) 775, 976
BshFI GGCC 4 cut(s) 42, 435, 774, 975
BshNI GGYRCC 2 cut(s) 475, 841
BsiEI CGRYCG 2 cut(s) 775, 976
BsiHKAI GWGCWC 2 cut(s) 160, 808
BsiSI CCGG 6 cut(s) 25, 33, 144, 224, 775, 976
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 910
BsnI GGCC 4 cut(s) 42, 435, 774, 975
Bsp119I TTCGAA 1 cut(s) 952
Bsp1286I GDGCHC 3 cut(s) 160, 496, 808
Bsp143I GATC 3 cut(s) 133, 164, 1027
BspACI CCGC 2 cut(s) 68, 972
BspANI GGCC 4 cut(s) 42, 435, 774, 975
BspCNI CTCAG 3 cut(s) 523, 589, 913
BspFNI CGCG 2 cut(s) 93, 972
BspLI GGNNCC 4 cut(s) 477, 520, 843, 909
BspMI ACCTGC 3 cut(s) 103, 901, 1044
BspT104I TTCGAA 1 cut(s) 952
BspT107I GGYRCC 2 cut(s) 475, 841
BsrFI RCCGGY 2 cut(s) 32, 143
BsrI ACTGG 2 cut(s) 366, 696
BssAI RCCGGY 2 cut(s) 32, 143
BssECI CCNNGG 2 cut(s) 5, 43
BssMI GATC 3 cut(s) 133, 164, 1027
BssNI GRCGYC 4 cut(s) 48, 141, 243, 476
Bst2UI CCWGG 1 cut(s) 964
Bst4CI ACNGT 6 cut(s) 13, 85, 321, 363, 733, 851
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 4 cut(s) 48, 141, 243, 476
BstAPI GCANNNNNTGC 1 cut(s) 803
BstBAI YACGTR 1 cut(s) 922
BstBI TTCGAA 1 cut(s) 952
BstC8I GCNNGC 4 cut(s) 95, 156, 373, 1067
BstDEI CTNAG 5 cut(s) 77, 531, 597, 900, 1059
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 436, 984
BstFNI CGCG 2 cut(s) 93, 972
BstH2I RGCGCY 2 cut(s) 33, 479
BstHHI GCGC 4 cut(s) 32, 93, 478, 1065
BstKTI GATC 3 cut(s) 136, 167, 1030
BstMBI GATC 3 cut(s) 133, 164, 1027
BstMCI CGRYCG 2 cut(s) 775, 976
BstMWI GCNNNNNNNGC 3 cut(s) 295, 803, 1050
BstNI CCWGG 1 cut(s) 964
BstNSI RCATGY 1 cut(s) 686
BstPAI GACNNNNGTC 1 cut(s) 144
BstSCI CCNGG 2 cut(s) 774, 962
BstSFI CTRYAG 1 cut(s) 1070
BstUI CGCG 2 cut(s) 93, 972
BstV1I GCAGC 4 cut(s) 491, 563, 901, 1021
BstV2I GAAGAC 1 cut(s) 60
BstZI CGGCCG 2 cut(s) 772, 973
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 4 cut(s) 42, 435, 774, 975
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 436, 984
BtsIMutI CAGTG 2 cut(s) 317, 373
BveI ACCTGC 3 cut(s) 103, 901, 1044
Cac8I GCNNGC 4 cut(s) 95, 156, 373, 1067
CfoI GCGC 4 cut(s) 32, 93, 478, 1065
Cfr10I RCCGGY 2 cut(s) 32, 143
Cfr13I GGNCC 2 cut(s) 9, 518
CseI GACGC 3 cut(s) 37, 149, 232
Csp6I GTAC 1 cut(s) 561
CviAII CATG 5 cut(s) 272, 437, 497, 523, 683
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 5 cut(s) 77, 531, 597, 900, 1059
DinI GGCGCC 1 cut(s) 477
DpnI GATC 3 cut(s) 135, 166, 1029
DpnII GATC 3 cut(s) 133, 164, 1027
EaeI YGGCCR 3 cut(s) 433, 772, 973
EagI CGGCCG 2 cut(s) 772, 973
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 158
EclXI CGGCCG 2 cut(s) 772, 973
Eco24I GRGCYC 2 cut(s) 160, 496
Eco47I GGWCC 2 cut(s) 9, 518
Eco52I CGGCCG 2 cut(s) 772, 973
Eco53kI GAGCTC 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 962
EcoT38I GRGCYC 2 cut(s) 160, 496
EgeI GGCGCC 1 cut(s) 477
EheI GGCGCC 1 cut(s) 477
FaeI CATG 5 cut(s) 275, 440, 500, 526, 686
FaqI GGGAC 1 cut(s) 910
FatI CATG 5 cut(s) 271, 436, 496, 522, 682
Fnu4HI GCNGC 5 cut(s) 505, 552, 915, 973, 1035
FokI GGATG 2 cut(s) 443, 991
FriOI GRGCYC 2 cut(s) 160, 496
Fsp4HI GCNGC 5 cut(s) 505, 552, 915, 973, 1035
FspBI CTAG 2 cut(s) 278, 564
GlaI GCGC 4 cut(s) 31, 92, 477, 1064
GluI GCNGC 5 cut(s) 505, 552, 915, 973, 1035
GsuI CTGGAG 1 cut(s) 1059
HaeII RGCGCY 2 cut(s) 33, 479
HaeIII GGCC 4 cut(s) 42, 435, 774, 975
HapII CCGG 6 cut(s) 25, 33, 144, 224, 775, 976
HgaI GACGC 3 cut(s) 37, 149, 232
HhaI GCGC 4 cut(s) 32, 93, 478, 1065
Hin1I GRCGYC 4 cut(s) 48, 141, 243, 476
Hin1II CATG 5 cut(s) 275, 440, 500, 526, 686
Hin6I GCGC 4 cut(s) 30, 91, 476, 1063
HinP1I GCGC 4 cut(s) 30, 91, 476, 1063
HincII GTYRAC 3 cut(s) 129, 149, 651
HindII GTYRAC 3 cut(s) 129, 149, 651
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 8 cut(s) 99, 125, 227, 406, 445, 873, 967, 1007
HpaII CCGG 6 cut(s) 25, 33, 144, 224, 775, 976
HphI GGTGA 1 cut(s) 863
Hpy166II GTNNAC 3 cut(s) 129, 149, 651
Hpy188I TCNGA 7 cut(s) 109, 164, 169, 232, 388, 689, 889
Hpy188III TCNNGA 5 cut(s) 137, 184, 410, 487, 902
Hpy8I GTNNAC 3 cut(s) 129, 149, 651
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 541, 826
HpyCH4III ACNGT 6 cut(s) 13, 85, 321, 363, 733, 851
HpyCH4IV ACGT 2 cut(s) 19, 921
HpyCH4V TGCA 8 cut(s) 271, 311, 507, 611, 725, 797, 917, 985
HpyF10VI GCNNNNNNNGC 3 cut(s) 295, 803, 1050
HpyF3I CTNAG 5 cut(s) 77, 531, 597, 900, 1059
HpySE526I ACGT 2 cut(s) 19, 921
Hsp92I GRCGYC 4 cut(s) 48, 141, 243, 476
Hsp92II CATG 5 cut(s) 275, 440, 500, 526, 686
HspAI GCGC 4 cut(s) 30, 91, 476, 1063
KasI GGCGCC 1 cut(s) 475
Kzo9I GATC 3 cut(s) 133, 164, 1027
LmnI GCTCC 2 cut(s) 27, 856
Lsp1109I GCAGC 4 cut(s) 491, 563, 901, 1021
LweI GCATC 3 cut(s) 298, 598, 784
MaeI CTAG 2 cut(s) 278, 564
MaeII ACGT 2 cut(s) 19, 921
MaeIII GTNAC 3 cut(s) 20, 343, 357
MalI GATC 3 cut(s) 135, 166, 1029
MboI GATC 3 cut(s) 133, 164, 1027
MboII GAAGA 7 cut(s) 65, 214, 629, 632, 688, 1001, 1016
MhlI GDGCHC 3 cut(s) 160, 496, 808
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 5 cut(s) 103, 422, 480, 739, 948
MluNI TGGCCA 1 cut(s) 435
Mly113I GGCGCC 1 cut(s) 476
MlyI GAGTC 2 cut(s) 93, 134
MmeI TCCRAC 1 cut(s) 712
Mox20I TGGCCA 1 cut(s) 435
MroXI GAANNNNTTC 1 cut(s) 958
MscI TGGCCA 1 cut(s) 435
MseI TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
MslI CAYNNNNRTG 1 cut(s) 341
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 554, 914
MspI CCGG 6 cut(s) 25, 33, 144, 224, 775, 976
MspR9I CCNGG 2 cut(s) 776, 964
MvaI CCWGG 1 cut(s) 964
MvnI CGCG 2 cut(s) 93, 972
MwoI GCNNNNNNNGC 3 cut(s) 295, 803, 1050
NarI GGCGCC 1 cut(s) 476
NciI CCSGG 1 cut(s) 776
NdeII GATC 3 cut(s) 133, 164, 1027
NlaIII CATG 5 cut(s) 275, 440, 500, 526, 686
NlaIV GGNNCC 4 cut(s) 477, 520, 843, 909
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 3 cut(s) 20, 343, 357
NspI RCATGY 1 cut(s) 686
NspV TTCGAA 1 cut(s) 952
OliI CACNNNNGTG 1 cut(s) 341
PciI ACATGT 1 cut(s) 682
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 565
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 6 cut(s) 227, 406, 445, 873, 967, 1007
PfoI TCCNGGA 1 cut(s) 962
PkrI GCNGC 5 cut(s) 506, 553, 916, 974, 1036
PleI GAGTC 2 cut(s) 93, 133
PluTI GGCGCC 1 cut(s) 479
PpsI GAGTC 2 cut(s) 93, 133
Ppu21I YACGTR 1 cut(s) 922
PscI ACATGT 1 cut(s) 682
PshAI GACNNNNGTC 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 962
PspGI CCWGG 1 cut(s) 962
PspN4I GGNNCC 4 cut(s) 477, 520, 843, 909
PspPI GGNCC 2 cut(s) 9, 518
PvuII CAGCTG 2 cut(s) 554, 914
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
SatI GCNGC 5 cut(s) 505, 552, 915, 973, 1035
Sau3AI GATC 3 cut(s) 133, 164, 1027
Sau96I GGNCC 2 cut(s) 9, 518
SchI GAGTC 2 cut(s) 93, 134
ScrFI CCNGG 2 cut(s) 776, 964
SduI GDGCHC 3 cut(s) 160, 496, 808
SfaNI GCATC 3 cut(s) 298, 598, 784
SfcI CTRYAG 1 cut(s) 1070
SfoI GGCGCC 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 952
SinI GGWCC 2 cut(s) 9, 518
SmiMI CAYNNNNRTG 1 cut(s) 341
Sse9I AATT 5 cut(s) 103, 422, 480, 739, 948
SsiI CCGC 2 cut(s) 68, 972
SspDI GGCGCC 1 cut(s) 475
SspI AATATT 1 cut(s) 705
SspMI CTAG 2 cut(s) 278, 564
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 2 cut(s) 774, 962
TaaI ACNGT 6 cut(s) 13, 85, 321, 363, 733, 851
TaiI ACGT 2 cut(s) 22, 924
TaqI TCGA 5 cut(s) 51, 409, 443, 448, 952
TasI AATT 5 cut(s) 103, 422, 480, 739, 948
TauI GCSGC 1 cut(s) 975
TfiI GAWTC 6 cut(s) 227, 406, 445, 873, 967, 1007
Tru1I TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
Tru9I TTAA 6 cut(s) 354, 425, 668, 701, 707, 765
TscAI CASTG 2 cut(s) 324, 373
TseFI GTSAC 3 cut(s) 20, 343, 357
TseI GCWGC 4 cut(s) 504, 551, 914, 1034
Tsp45I GTSAC 3 cut(s) 20, 343, 357
TspDTI ATGAA 5 cut(s) 192, 382, 539, 744, 845
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 2 cut(s) 324, 373
VpaK11BI GGWCC 2 cut(s) 9, 518
XapI RAATTY 1 cut(s) 739
XceI RCATGY 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 2 cut(s) 278, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.