Rh5DG554800

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000940
Physical Location & Seq
Forward (+)
85688794 .. 85689789
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG554800.1

Sequence Viewer

Length: 648 bp
ATGAGTCCGGATTCGATGAAGTTTGATGTGATCATTGATATAGTTGACAAACTGCATGAGCAAGTTCAGCTTCCGAGAGAACAAGTAGCGGATGCACAAGCACTTCTGGACATAAGTAACACAGTGGTGGCCTCTGTTATGTCACATTCTAATGGGGAAACTACCCCAAGCGATTTCATTACTGGTTTGATTACATATTTCGGGCAAGTCAATAGGCCATCAGTGAACAAAGATAATGTCCATGTCTCACTTAAATGGAAAGATATTGGACTTTCTGTTTCACCAATACTCAAGAGGGCTGTTGGATGTTGCACAATGCTTGGACCCATGAATATTCAAATTAAGCAAAGGAAGGCTGCTGTCCGGCGTACTAAGCCTACCACAACTCATCTGCCCAAGGAGATCAATAATGAAGGAGGCGAAAGAACTGATACAGATTTGAACATGGCAACAATGTTTGAGATTTTAAAGAACAAGAACAGGGTGGAATTCAGTTGTTTGATACTAAATAGAAGATCTTTTGCACAGACAGTGGAGAATCTATTTGCTCTGTCATTTCTGGCAAAAGACGGCAGAGTTAGAATTGCTGTCGAGCAAATGGTTCTCATATTGTTTGTAAGTGGAATAGAAAAACAACACAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

215

Amino Acids

24.08

Weight (kDa)

9.01

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 162 - 200 3.2e-12 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 1 cut(s) 89
AcsI RAATTY 2 cut(s) 488, 642
AfaI GTAC 1 cut(s) 370
AgsI TTSAA 2 cut(s) 338, 442
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 250
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 2 cut(s) 129, 215
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 488, 642
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 273
AvaII GGWCC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 343
BccI CCATC 1 cut(s) 226
BceAI ACGGC 1 cut(s) 586
BclI TGATCA 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 250
BglII AGATCT 1 cut(s) 515
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 1 cut(s) 325
BmsI GCATC 1 cut(s) 82
BpuEI CTTGAG 1 cut(s) 275
BsaJI CCNNGG 1 cut(s) 396
BsaWI WCCGGW 1 cut(s) 7
Bse1I ACTGG 1 cut(s) 187
BseAI TCCGGA 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 396
BseGI GGATG 2 cut(s) 97, 311
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 343
BshFI GGCC 2 cut(s) 131, 217
BsiSI CCGG 2 cut(s) 8, 364
BsmAI GTCTC 1 cut(s) 250
BsnI GGCC 2 cut(s) 131, 217
Bsp13I TCCGGA 1 cut(s) 7
Bsp143I GATC 3 cut(s) 30, 402, 515
BspACI CCGC 1 cut(s) 89
BspANI GGCC 2 cut(s) 131, 217
BspEI TCCGGA 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 325
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 3 cut(s) 30, 402, 515
BssT1I CCWWGG 1 cut(s) 396
Bst4CI ACNGT 2 cut(s) 124, 532
BstDEI CTNAG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 97, 311
BstKTI GATC 3 cut(s) 33, 405, 518
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 3 cut(s) 30, 402, 515
BstMWI GCNNNNNNNGC 2 cut(s) 67, 373
BstV1I GCAGC 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 515
BstYI RGATCY 1 cut(s) 515
BsuRI GGCC 2 cut(s) 131, 217
BtsCI GGATG 2 cut(s) 97, 311
BtsIMutI CAGTG 3 cut(s) 129, 228, 537
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 369
CviAII CATG 4 cut(s) 56, 242, 328, 445
CviJI RGCY 6 cut(s) 70, 131, 217, 299, 356, 376
CviKI_1 RGCY 6 cut(s) 70, 131, 217, 299, 356, 376
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 1 cut(s) 372
DpnI GATC 3 cut(s) 32, 404, 517
DpnII GATC 3 cut(s) 30, 402, 515
DraI TTTAAA 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 396
FaeI CATG 4 cut(s) 59, 245, 331, 448
FaiI YATR 9 cut(s) 41, 57, 113, 140, 196, 243, 329, 446, 608
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FatI CATG 4 cut(s) 55, 241, 327, 444
FbaI TGATCA 1 cut(s) 30
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 104, 318
Fsp4HI GCNGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 357
HaeIII GGCC 2 cut(s) 131, 217
HapII CCGG 2 cut(s) 8, 364
Hin1II CATG 4 cut(s) 59, 245, 331, 448
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 3 cut(s) 4, 11, 538
HpaII CCGG 2 cut(s) 8, 364
HphI GGTGA 1 cut(s) 273
Hpy166II GTNNAC 2 cut(s) 46, 226
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 3 cut(s) 8, 107, 292
Hpy8I GTNNAC 2 cut(s) 46, 226
HpyAV CCTTC 2 cut(s) 346, 407
HpyCH4III ACNGT 2 cut(s) 124, 532
HpyCH4V TGCA 4 cut(s) 55, 95, 312, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 373
HpyF3I CTNAG 1 cut(s) 372
Hsp92II CATG 4 cut(s) 59, 245, 331, 448
Kpn2I TCCGGA 1 cut(s) 7
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 3 cut(s) 30, 402, 515
LpnPI CCDG 6 cut(s) 21, 92, 168, 377, 466, 545
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 82
MaeIII GTNAC 2 cut(s) 116, 141
MalI GATC 3 cut(s) 32, 404, 517
MboI GATC 3 cut(s) 30, 402, 515
MboII GAAGA 1 cut(s) 525
MflI RGATCY 1 cut(s) 515
MluCI AATT 4 cut(s) 339, 488, 582, 642
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 3 cut(s) 142, 288, 410
MroI TCCGGA 1 cut(s) 7
MseI TTAA 4 cut(s) 252, 342, 467, 646
MslI CAYNNNNRTG 3 cut(s) 125, 150, 253
MspI CCGG 2 cut(s) 8, 364
MwoI GCNNNNNNNGC 2 cut(s) 67, 373
NdeII GATC 3 cut(s) 30, 402, 515
NlaIII CATG 4 cut(s) 59, 245, 331, 448
NlaIV GGNNCC 1 cut(s) 325
NmuCI GTSAC 1 cut(s) 141
OliI CACNNNNGTG 1 cut(s) 125
PfeI GAWTC 2 cut(s) 11, 538
PkrI GCNGC 1 cut(s) 358
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 1 cut(s) 323
PsuI RGATCY 1 cut(s) 515
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
RseI CAYNNNNRTG 3 cut(s) 125, 150, 253
SaqAI TTAA 4 cut(s) 252, 342, 467, 646
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 3 cut(s) 30, 402, 515
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 1 cut(s) 13
SetI ASST 1 cut(s) 72
SfaNI GCATC 1 cut(s) 82
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 3 cut(s) 125, 150, 253
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 4 cut(s) 339, 488, 582, 642
SsiI CCGC 1 cut(s) 89
SspI AATATT 1 cut(s) 334
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 2 cut(s) 124, 532
TaqI TCGA 2 cut(s) 14, 591
TasI AATT 4 cut(s) 339, 488, 582, 642
TfiI GAWTC 2 cut(s) 11, 538
Tru1I TTAA 4 cut(s) 252, 342, 467, 646
Tru9I TTAA 4 cut(s) 252, 342, 467, 646
TscAI CASTG 3 cut(s) 129, 228, 537
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 32, 166, 344, 426
TspRI CASTG 3 cut(s) 129, 228, 537
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 2 cut(s) 488, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.