FvH4_3g44140

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000940
Physical Location & Seq
Forward (+)
36805335 .. 36808118
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44140.t2

Sequence Viewer

Length: 1155 bp
ATGCCGAAGTCCACGAAACGCGACCGGAGCGCCGCCCAAGGCCGCGGCGTCGACGACGCCGAGGAGGCCGAGCGTCTCCGATCCGTGAAGCGCGAGCGGCTCAACCCTGAACAGGATGAGAATGAGTCCACCGAGCAAGACGCCGGTCAACGGCGAGCTCTCCGATCTGACTACAATGATATCCTCAACCTCATCAGCGAGAAGAGGAACGATTTATTGAGACCGGAGTCGGAGAAATTTGGGGTTATTATTGATAGAGTTGAGAGGCTGCATAAGCAAGTTCAGAAGCCAAGGGAGCAAGTGGCAGACGCAGAGGCACTGTTGGACATAACCAACACACTGGTGACATCTGTGAAGTCACAGTCCAGTGCTGGTATTACCCCGGCGGACTTCATTACCTGTTTGATTCAAGATTTTGGGCAGTTAAGGGACGGGAATGCCTTGATTCGGTGGAAGGATGTTGGGCTTGGTGTGGCGCCAATTTTCAGGAGGGCTCATGGGTGCTGTACAATGCTTGGACCCATGAATACCGAGTATAAGGAAAGGAAGGTTGGTGTTCGTACTAGACGAACAAAGCCTACTACGACTGATGAGCCTGAGGAGATTGATGCACAGGAAGAAGAAAAAACTGATACGGACAGGAATATGTCAACTATGTTTGAGATTTTAAGAAAGAACAAACGCGTACGACTGGAATGGTTAATATTAAACAGACTGTTCTTTGCACAGACAGTGGAGAATTTATTTGCTCTTTCATTCCTGGTTAAAGATGGCCGGGCTGAAATCATAGTTGATGCAAATTGTTGTCATTTTGTTGCACCAAGGAATGCTGCCAAGGCCACTGAAGTGGCATCTCGTGAGGTGGTTTATAGACACTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGTTGATGAAGGATATGGTGCCCGATGGTGAGGAGCTAATGCCACATAGAGTCCCTCCCAACTCTGAACCTGCCTCACAGGAGGAACCGGCTGCATACAGTTCCCAAACAGGCCACACAACTCCAATTAGGAAGCTTTCCAGGAATCGTGGCCGGGTTGTGCAGGAAGAAGCTGTTGTAGAAGAATCCCCTGAATGTGATAATGGTCATGCTGGAGGCACATTTCGCAGGTCTAAGCGCAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

385

Amino Acids

43.88

Weight (kDa)

7.07

Isoelectric Point (pI)

61.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 985, 1125
AccB1I GGYRCC 2 cut(s) 475, 925
AccBSI CCGCTC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 51
AccII CGCG 4 cut(s) 21, 45, 93, 684
AciI CCGC 5 cut(s) 33, 43, 45, 97, 386
AclWI GGATC 1 cut(s) 75
AcoI YGGCCR 2 cut(s) 772, 1057
AcsI RAATTY 2 cut(s) 236, 739
AcuI CTGAAG 1 cut(s) 864
AcyI GRCGYC 4 cut(s) 48, 57, 141, 476
AdeI CACNNNGTG 1 cut(s) 880
AfaI GTAC 3 cut(s) 508, 562, 687
AfiI CCNNNNNNNGG 3 cut(s) 112, 150, 447
AflIII ACRYGT 1 cut(s) 682
AgsI TTSAA 2 cut(s) 410, 898
AjnI CCWGG 2 cut(s) 759, 1046
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
AleI CACNNNNGTG 2 cut(s) 341, 845
AluBI AGCT 5 cut(s) 158, 943, 1042, 1079, 1150
AluI AGCT 5 cut(s) 158, 943, 1042, 1079, 1150
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 80, 214
AlwI GGATC 1 cut(s) 75
AoxI GGCC 6 cut(s) 40, 66, 772, 837, 1018, 1057
ApeKI GCWGC 3 cut(s) 268, 830, 998
ApoI RAATTY 2 cut(s) 236, 739
AspLEI GCGC 4 cut(s) 32, 93, 478, 1146
AspS9I GGNCC 1 cut(s) 518
AsuC2I CCSGG 3 cut(s) 383, 776, 1061
AsuHPI GGTGA 2 cut(s) 355, 947
AvaII GGWCC 1 cut(s) 518
AxyI CCTNAGG 1 cut(s) 597
BaeGI GKGCMC 1 cut(s) 930
BanI GGYRCC 2 cut(s) 475, 925
BanII GRGCYC 2 cut(s) 160, 496
BauI CACGAG 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 255, 817, 985
BccI CCATC 2 cut(s) 764, 926
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 761, 1048
BcnI CCSGG 3 cut(s) 383, 776, 1061
BcoDI GTCTC 2 cut(s) 80, 214
BfaI CTAG 1 cut(s) 564
BfoI RGCGCY 2 cut(s) 33, 479
BfuAI ACCTGC 2 cut(s) 985, 1125
BglI GCCNNNNNGGC 1 cut(s) 65
BisI GCNGC 7 cut(s) 33, 43, 46, 98, 269, 831, 999
BlsI GCNGC 7 cut(s) 34, 44, 47, 99, 270, 832, 1000
Bme1390I CCNGG 5 cut(s) 383, 761, 776, 1048, 1061
Bme18I GGWCC 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 4 cut(s) 477, 520, 927, 993
BmrFI CCNGG 5 cut(s) 383, 761, 776, 1048, 1061
BmsI GCATC 3 cut(s) 598, 784, 860
BoxI GACNNNNGTC 2 cut(s) 144, 226
BpmI CTGGAG 1 cut(s) 1140
BpuMI CCSGG 3 cut(s) 383, 776, 1061
BsaHI GRCGYC 4 cut(s) 48, 57, 141, 476
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 7 cut(s) 37, 43, 60, 290, 381, 821, 834
BsaWI WCCGGW 2 cut(s) 24, 223
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 150, 447
Bse118I RCCGGY 2 cut(s) 143, 994
Bse1I ACTGG 4 cut(s) 345, 366, 696, 908
Bse21I CCTNAGG 1 cut(s) 597
BseBI CCWGG 2 cut(s) 761, 1048
BseDI CCNNGG 7 cut(s) 37, 43, 60, 290, 381, 821, 834
BseGI GGATG 2 cut(s) 121, 463
BseLI CCNNNNNNNGG 3 cut(s) 112, 150, 447
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 4 cut(s) 345, 366, 696, 908
BseRI GAGGAG 3 cut(s) 77, 614, 953
BseSI GKGCMC 1 cut(s) 930
BseXI GCAGC 3 cut(s) 255, 817, 985
BsgI GTGCAG 1 cut(s) 1088
Bsh1236I CGCG 4 cut(s) 21, 45, 93, 684
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 6 cut(s) 42, 68, 774, 839, 1020, 1059
BshNI GGYRCC 2 cut(s) 475, 925
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 7 cut(s) 25, 144, 224, 383, 775, 995, 1060
BsiWI CGTACG 1 cut(s) 685
BslFI GGGAC 2 cut(s) 443, 944
BslI CCNNNNNNNGG 3 cut(s) 112, 150, 447
BsmAI GTCTC 2 cut(s) 80, 214
BsmBI CGTCTC 1 cut(s) 80
BsmFI GGGAC 2 cut(s) 443, 944
BsmI GAATGC 2 cut(s) 442, 832
BsnI GGCC 6 cut(s) 42, 68, 774, 839, 1020, 1059
Bso31I GGTCTC 1 cut(s) 214
Bsp1286I GDGCHC 3 cut(s) 160, 496, 930
Bsp1407I TGTACA 1 cut(s) 506
Bsp143I GATC 2 cut(s) 80, 164
BspACI CCGC 5 cut(s) 33, 43, 45, 97, 386
BspANI GGCC 6 cut(s) 42, 68, 774, 839, 1020, 1059
BspCNI CTCAG 1 cut(s) 589
BspFNI CGCG 4 cut(s) 21, 45, 93, 684
BspLI GGNNCC 4 cut(s) 477, 520, 927, 993
BspMI ACCTGC 2 cut(s) 985, 1125
BspPI GGATC 1 cut(s) 75
BspT107I GGYRCC 2 cut(s) 475, 925
BspTNI GGTCTC 1 cut(s) 214
BsrBI CCGCTC 1 cut(s) 97
BsrFI RCCGGY 2 cut(s) 143, 994
BsrGI TGTACA 1 cut(s) 506
BsrI ACTGG 4 cut(s) 345, 366, 696, 908
BssAI RCCGGY 2 cut(s) 143, 994
BssECI CCNNGG 7 cut(s) 37, 43, 60, 290, 381, 821, 834
BssMI GATC 2 cut(s) 80, 164
BssNI GRCGYC 4 cut(s) 48, 57, 141, 476
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 4 cut(s) 37, 290, 821, 834
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 2 cut(s) 761, 1048
Bst4CI ACNGT 5 cut(s) 321, 363, 717, 733, 1007
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 4 cut(s) 48, 57, 141, 476
BstAUI TGTACA 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 95, 156, 1148
BstDEI CTNAG 2 cut(s) 597, 1140
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 121, 463
BstFNI CGCG 4 cut(s) 21, 45, 93, 684
BstH2I RGCGCY 2 cut(s) 33, 479
BstHHI GCGC 4 cut(s) 32, 93, 478, 1146
BstKTI GATC 2 cut(s) 83, 167
BstMAI GTCTC 2 cut(s) 80, 214
BstMBI GATC 2 cut(s) 80, 164
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 7 cut(s) 27, 65, 97, 274, 295, 836, 1131
BstNI CCWGG 2 cut(s) 761, 1048
BstPAI GACNNNNGTC 2 cut(s) 144, 226
BstSCI CCNGG 5 cut(s) 381, 759, 774, 1046, 1059
BstSLI GKGCMC 1 cut(s) 930
BstUI CGCG 4 cut(s) 21, 45, 93, 684
BstV1I GCAGC 3 cut(s) 255, 817, 985
BstXI CCANNNNNNTGG 2 cut(s) 340, 847
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 6 cut(s) 42, 68, 774, 839, 1020, 1059
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 121, 463
BtsIMutI CAGTG 5 cut(s) 317, 338, 373, 738, 840
BveI ACCTGC 2 cut(s) 985, 1125
Cac8I GCNNGC 3 cut(s) 95, 156, 1148
CfoI GCGC 4 cut(s) 32, 93, 478, 1146
Cfr10I RCCGGY 2 cut(s) 143, 994
Cfr13I GGNCC 1 cut(s) 518
Cfr42I CCGCGG 1 cut(s) 46
CseI GACGC 5 cut(s) 37, 62, 65, 149, 317
Csp6I GTAC 3 cut(s) 507, 561, 686
CviAII CATG 3 cut(s) 497, 523, 1115
CviQI GTAC 3 cut(s) 507, 561, 686
DdeI CTNAG 2 cut(s) 597, 1140
DinI GGCGCC 1 cut(s) 477
DpnI GATC 2 cut(s) 82, 166
DpnII GATC 2 cut(s) 80, 164
DraIII CACNNNGTG 1 cut(s) 880
EaeI YGGCCR 2 cut(s) 772, 1057
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 4 cut(s) 37, 290, 821, 834
Eco24I GRGCYC 2 cut(s) 160, 496
Eco31I GGTCTC 1 cut(s) 214
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 518
Eco53kI GAGCTC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 864
Eco81I CCTNAGG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 759, 1046
EcoRV GATATC 1 cut(s) 181
EcoT14I CCWWGG 4 cut(s) 37, 290, 821, 834
EcoT38I GRGCYC 2 cut(s) 160, 496
EgeI GGCGCC 1 cut(s) 477
EheI GGCGCC 1 cut(s) 477
ErhI CCWWGG 4 cut(s) 37, 290, 821, 834
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 3 cut(s) 500, 526, 1118
FaqI GGGAC 2 cut(s) 443, 944
FatI CATG 3 cut(s) 496, 522, 1114
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 7 cut(s) 33, 43, 46, 98, 269, 831, 999
FokI GGATG 2 cut(s) 128, 470
FriOI GRGCYC 2 cut(s) 160, 496
Fsp4HI GCNGC 7 cut(s) 33, 43, 46, 98, 269, 831, 999
FspBI CTAG 1 cut(s) 564
GlaI GCGC 4 cut(s) 31, 92, 477, 1145
GluI GCNGC 7 cut(s) 33, 43, 46, 98, 269, 831, 999
GsuI CTGGAG 1 cut(s) 1140
HaeII RGCGCY 2 cut(s) 33, 479
HaeIII GGCC 6 cut(s) 42, 68, 774, 839, 1020, 1059
HapII CCGG 7 cut(s) 25, 144, 224, 383, 775, 995, 1060
HgaI GACGC 5 cut(s) 37, 62, 65, 149, 317
HhaI GCGC 4 cut(s) 32, 93, 478, 1146
Hin1I GRCGYC 4 cut(s) 48, 57, 141, 476
Hin1II CATG 3 cut(s) 500, 526, 1118
Hin6I GCGC 4 cut(s) 30, 91, 476, 1144
HinP1I GCGC 4 cut(s) 30, 91, 476, 1144
HincII GTYRAC 3 cut(s) 52, 149, 651
HindII GTYRAC 3 cut(s) 52, 149, 651
HindIII AAGCTT 2 cut(s) 1040, 1148
HinfI GANTC 7 cut(s) 125, 227, 406, 445, 957, 1051, 1091
HpaII CCGG 7 cut(s) 25, 144, 224, 383, 775, 995, 1060
HphI GGTGA 2 cut(s) 355, 947
Hpy166II GTNNAC 5 cut(s) 12, 52, 129, 149, 651
Hpy188I TCNGA 7 cut(s) 80, 164, 169, 232, 285, 887, 973
Hpy188III TCNNGA 3 cut(s) 410, 487, 857
Hpy8I GTNNAC 5 cut(s) 12, 52, 129, 149, 651
Hpy99I CGWCG 3 cut(s) 53, 56, 59
HpyAV CCTTC 3 cut(s) 448, 541, 910
HpyCH4III ACNGT 5 cut(s) 321, 363, 717, 733, 1007
HpyCH4V TGCA 7 cut(s) 271, 611, 725, 797, 818, 1001, 1069
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 65, 97, 274, 295, 836, 1131
HpyF3I CTNAG 2 cut(s) 597, 1140
Hsp92I GRCGYC 4 cut(s) 48, 57, 141, 476
Hsp92II CATG 3 cut(s) 500, 526, 1118
HspAI GCGC 4 cut(s) 30, 91, 476, 1144
KasI GGCGCC 1 cut(s) 475
KspI CCGCGG 1 cut(s) 46
Kzo9I GATC 2 cut(s) 80, 164
LmnI GCTCC 3 cut(s) 27, 295, 940
Lsp1109I GCAGC 3 cut(s) 255, 817, 985
LweI GCATC 3 cut(s) 598, 784, 860
MaeI CTAG 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 343, 357
MalI GATC 2 cut(s) 82, 166
MbiI CCGCTC 1 cut(s) 97
MboI GATC 2 cut(s) 80, 164
MboII GAAGA 5 cut(s) 214, 629, 632, 1085, 1100
MhlI GDGCHC 3 cut(s) 160, 496, 930
MluCI AATT 5 cut(s) 236, 480, 739, 799, 1032
MluI ACGCGT 1 cut(s) 682
Mly113I GGCGCC 1 cut(s) 476
MlyI GAGTC 3 cut(s) 134, 236, 966
MmeI TCCRAC 2 cut(s) 210, 303
MseI TTAA 5 cut(s) 425, 668, 701, 707, 765
MslI CAYNNNNRTG 2 cut(s) 341, 845
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 7 cut(s) 25, 144, 224, 383, 775, 995, 1060
MspR9I CCNGG 5 cut(s) 383, 761, 776, 1048, 1061
Mva1269I GAATGC 2 cut(s) 442, 832
MvaI CCWGG 2 cut(s) 761, 1048
MvnI CGCG 4 cut(s) 21, 45, 93, 684
MwoI GCNNNNNNNGC 7 cut(s) 27, 65, 97, 274, 295, 836, 1131
NarI GGCGCC 1 cut(s) 476
NciI CCSGG 3 cut(s) 383, 776, 1061
NdeII GATC 2 cut(s) 80, 164
NlaIII CATG 3 cut(s) 500, 526, 1118
NlaIV GGNNCC 4 cut(s) 477, 520, 927, 993
NmeAIII GCCGAG 2 cut(s) 85, 94
NmuCI GTSAC 2 cut(s) 343, 357
OliI CACNNNNGTG 2 cut(s) 341, 845
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 565
PctI GAATGC 2 cut(s) 442, 832
PfeI GAWTC 4 cut(s) 406, 445, 1051, 1091
Pfl23II CGTACG 1 cut(s) 685
PfoI TCCNGGA 1 cut(s) 1046
PkrI GCNGC 7 cut(s) 34, 44, 47, 99, 270, 832, 1000
PleI GAGTC 3 cut(s) 133, 235, 965
PluTI GGCGCC 1 cut(s) 479
PpsI GAGTC 3 cut(s) 133, 235, 965
PshAI GACNNNNGTC 2 cut(s) 144, 226
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 2 cut(s) 759, 1046
PspGI CCWGG 2 cut(s) 759, 1046
PspLI CGTACG 1 cut(s) 685
PspN4I GGNNCC 4 cut(s) 477, 520, 927, 993
PspPI GGNCC 1 cut(s) 518
PsrI GAACNNNNNNTAC 2 cut(s) 562, 594
RsaI GTAC 3 cut(s) 508, 562, 687
RsaNI GTAC 3 cut(s) 507, 561, 686
RseI CAYNNNNRTG 2 cut(s) 341, 845
SacI GAGCTC 1 cut(s) 160
SacII CCGCGG 1 cut(s) 46
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 5 cut(s) 425, 668, 701, 707, 765
SatI GCNGC 7 cut(s) 33, 43, 46, 98, 269, 831, 999
Sau3AI GATC 2 cut(s) 80, 164
Sau96I GGNCC 1 cut(s) 518
SchI GAGTC 3 cut(s) 134, 236, 966
ScrFI CCNGG 5 cut(s) 383, 761, 776, 1048, 1061
SduI GDGCHC 3 cut(s) 160, 496, 930
SfaNI GCATC 3 cut(s) 598, 784, 860
SfoI GGCGCC 1 cut(s) 477
Sfr303I CCGCGG 1 cut(s) 46
SgrBI CCGCGG 1 cut(s) 46
SgrDI CGTCGACG 1 cut(s) 50
SinI GGWCC 1 cut(s) 518
SmiMI CAYNNNNRTG 2 cut(s) 341, 845
Sse9I AATT 5 cut(s) 236, 480, 739, 799, 1032
SsiI CCGC 5 cut(s) 33, 43, 45, 97, 386
SspDI GGCGCC 1 cut(s) 475
SspI AATATT 1 cut(s) 705
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 5 cut(s) 381, 759, 774, 1046, 1059
StyI CCWWGG 4 cut(s) 37, 290, 821, 834
TaaI ACNGT 5 cut(s) 321, 363, 717, 733, 1007
TaqI TCGA 1 cut(s) 51
TasI AATT 5 cut(s) 236, 480, 739, 799, 1032
TatI WGTACW 1 cut(s) 506
TauI GCSGC 4 cut(s) 35, 45, 48, 100
TfiI GAWTC 4 cut(s) 406, 445, 1051, 1091
Tru1I TTAA 5 cut(s) 425, 668, 701, 707, 765
Tru9I TTAA 5 cut(s) 425, 668, 701, 707, 765
TscAI CASTG 5 cut(s) 324, 345, 373, 738, 847
TseFI GTSAC 2 cut(s) 343, 357
TseI GCWGC 3 cut(s) 268, 830, 998
Tsp45I GTSAC 2 cut(s) 343, 357
TspDTI ATGAA 4 cut(s) 382, 539, 744, 929
TspGWI ACGGA 2 cut(s) 73, 650
TspRI CASTG 5 cut(s) 324, 345, 373, 738, 847
VpaK11BI GGWCC 1 cut(s) 518
XapI RAATTY 2 cut(s) 236, 739
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.