Rh5CG568000

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000940
Physical Location & Seq
Forward (+)
80418596 .. 80420897
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG568000.1

Sequence Viewer

Length: 852 bp
ATGCCGAGGACCGCGAAACGCGACCGGAGCGCCGGTCAAGGCCGTGGCGTCGATGACGGCGAGGAGGCGGAGCGGCTCCGAGCCGTGAAGCGCGAGCGACTCAATTCCGAGCAGGTTGAAAATGAGTCAACCGATCAGGACGCCGGTCAACGGCGAGCTCTCCGATCTGGCTACAATGAAATCCTGAACCTCATTAGTGAGAAGAGAAATGAGTTATTGAAGCCGGAGTCGGAGAAATTTGGCGTCATCATTGGTAGAGTTGAGAAGTTGCATGAGCTAGTTCAGAAGCCAAGAGAGCAAGTGGCAGATGCAGAGGCACTTTTGGACATAACTAACACATTGGTAACCTCTGTGAAGTCACAGTCCAGTGCCGGCATTACTCCATCAGACTTCATTACCTGTTTGATTCGAGATTTTGGACAATTAAGGGATGGCCATGCCTCGATTCGGTGGAAAGACGTTGGGGTGGCCGTGGCACCAATTTTCAAGAGGGCTCATGGGTGCTGTACAATGCTTGGACCCATGAATACTGAGTATAAGGAAAGGAAGGCAGCTGTTCGTACTAGACGAACAAAGCCTACCACAACTGATGAGCCTGAGGAGTTGATGAAGGATATGGTGCCTGATGGTGAGGAGCTAATGCCACATAGAATCCCTGCCAACTCTGAACCTGCCTCTCAGGAGGAACCAGCTGCATACGTGTCCCAAACAGGACACACAACTCCAATTCGAAAGCTTTCCAGGAATCGCGGCAGGATGGTGCAGGAAGAAGCTATTGTAGAAGAATCCCCTGAATATGATAATGACCGTGCTGCTGGAGGCGCACTTCGCAGGTCTAAGCGCAAGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

283

Amino Acids

31.73

Weight (kDa)

7.73

Isoelectric Point (pI)

58.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 103, 679, 822
AccB1I GGYRCC 2 cut(s) 475, 619
AccBSI CCGCTC 1 cut(s) 73
AccII CGCG 4 cut(s) 14, 21, 93, 750
AciI CCGC 4 cut(s) 12, 68, 73, 750
AcoI YGGCCR 2 cut(s) 433, 468
AcsI RAATTY 1 cut(s) 236
AcyI GRCGYC 3 cut(s) 48, 141, 243
AfaI GTAC 2 cut(s) 508, 562
AfiI CCNNNNNNNGG 2 cut(s) 150, 447
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 3 cut(s) 119, 220, 487
AjnI CCWGG 1 cut(s) 740
AluBI AGCT 8 cut(s) 158, 277, 554, 637, 692, 736, 773, 847
AluI AGCT 8 cut(s) 158, 277, 554, 637, 692, 736, 773, 847
Alw21I GWGCWC 1 cut(s) 160
AoxI GGCC 3 cut(s) 40, 433, 468
ApeKI GCWGC 3 cut(s) 551, 692, 812
ApoI RAATTY 1 cut(s) 236
Asp700I GAANNNNTTC 1 cut(s) 736
AspLEI GCGC 4 cut(s) 32, 93, 824, 843
AspS9I GGNCC 2 cut(s) 9, 518
AsuHPI GGTGA 1 cut(s) 641
AsuII TTCGAA 1 cut(s) 730
AvaII GGWCC 2 cut(s) 9, 518
AxyI CCTNAGG 1 cut(s) 597
BalI TGGCCA 1 cut(s) 435
BanI GGYRCC 2 cut(s) 475, 619
BanII GRGCYC 2 cut(s) 160, 496
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 563, 679, 799
BccI CCATC 4 cut(s) 391, 425, 620, 751
BceAI ACGGC 5 cut(s) 27, 68, 73, 167, 455
BciT130I CCWGG 1 cut(s) 742
BfaI CTAG 2 cut(s) 278, 564
BfmI CTRYAG 1 cut(s) 848
BfoI RGCGCY 1 cut(s) 33
BfuAI ACCTGC 3 cut(s) 103, 679, 822
BisI GCNGC 5 cut(s) 74, 552, 693, 751, 813
BlsI GCNGC 5 cut(s) 75, 553, 694, 752, 814
Bme1390I CCNGG 1 cut(s) 742
Bme18I GGWCC 2 cut(s) 9, 518
BmgT120I GGNCC 2 cut(s) 9, 518
BmiI GGNNCC 5 cut(s) 77, 477, 520, 621, 687
BmrFI CCNGG 1 cut(s) 742
BmsI GCATC 1 cut(s) 298
BoxI GACNNNNGTC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 837
Bpu14I TTCGAA 1 cut(s) 730
BsaAI YACGTR 1 cut(s) 700
BsaHI GRCGYC 3 cut(s) 48, 141, 243
BsaJI CCNNGG 3 cut(s) 5, 43, 471
BsaWI WCCGGW 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 19, 49
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 447
Bse118I RCCGGY 3 cut(s) 32, 143, 371
Bse1I ACTGG 1 cut(s) 366
Bse21I CCTNAGG 1 cut(s) 597
BseBI CCWGG 1 cut(s) 742
BseDI CCNNGG 3 cut(s) 5, 43, 471
BseGI GGATG 2 cut(s) 436, 762
BseLI CCNNNNNNNGG 2 cut(s) 150, 447
BseMII CTCAG 3 cut(s) 522, 588, 692
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 3 cut(s) 77, 614, 647
BseXI GCAGC 3 cut(s) 563, 679, 799
BsgI GTGCAG 1 cut(s) 782
Bsh1236I CGCG 4 cut(s) 14, 21, 93, 750
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 3 cut(s) 42, 435, 470
BshNI GGYRCC 2 cut(s) 475, 619
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 5 cut(s) 25, 33, 144, 224, 372
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 2 cut(s) 150, 447
BsmFI GGGAC 1 cut(s) 688
BsnI GGCC 3 cut(s) 42, 435, 470
Bsp119I TTCGAA 1 cut(s) 730
Bsp1286I GDGCHC 2 cut(s) 160, 496
Bsp1407I TGTACA 1 cut(s) 506
Bsp143I GATC 2 cut(s) 133, 164
BspACI CCGC 4 cut(s) 12, 68, 73, 750
BspANI GGCC 3 cut(s) 42, 435, 470
BspCNI CTCAG 3 cut(s) 523, 589, 691
BspFNI CGCG 4 cut(s) 14, 21, 93, 750
BspLI GGNNCC 5 cut(s) 77, 477, 520, 621, 687
BspMI ACCTGC 3 cut(s) 103, 679, 822
BspT104I TTCGAA 1 cut(s) 730
BspT107I GGYRCC 2 cut(s) 475, 619
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 3 cut(s) 32, 143, 371
BsrGI TGTACA 1 cut(s) 506
BsrI ACTGG 1 cut(s) 366
BssAI RCCGGY 3 cut(s) 32, 143, 371
BssECI CCNNGG 3 cut(s) 5, 43, 471
BssMI GATC 2 cut(s) 133, 164
BssNI GRCGYC 3 cut(s) 48, 141, 243
Bst2UI CCWGG 1 cut(s) 742
Bst4CI ACNGT 2 cut(s) 363, 809
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 3 cut(s) 48, 141, 243
BstAUI TGTACA 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 700
BstBI TTCGAA 1 cut(s) 730
BstC8I GCNNGC 4 cut(s) 95, 156, 373, 845
BstDEI CTNAG 4 cut(s) 531, 597, 678, 837
BstDSI CCRYGG 2 cut(s) 43, 471
BstEII GGTNACC 1 cut(s) 343
BstF5I GGATG 2 cut(s) 436, 762
BstFNI CGCG 4 cut(s) 14, 21, 93, 750
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 4 cut(s) 32, 93, 824, 843
BstKTI GATC 2 cut(s) 136, 167
BstMBI GATC 2 cut(s) 133, 164
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 4 cut(s) 27, 295, 821, 828
BstNI CCWGG 1 cut(s) 742
BstPAI GACNNNNGTC 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 740
BstSFI CTRYAG 1 cut(s) 848
BstUI CGCG 4 cut(s) 14, 21, 93, 750
BstV1I GCAGC 3 cut(s) 563, 679, 799
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 3 cut(s) 42, 435, 470
BtgI CCRYGG 2 cut(s) 43, 471
BtsCI GGATG 2 cut(s) 436, 762
BtsIMutI CAGTG 1 cut(s) 373
BveI ACCTGC 3 cut(s) 103, 679, 822
Cac8I GCNNGC 4 cut(s) 95, 156, 373, 845
CfoI GCGC 4 cut(s) 32, 93, 824, 843
Cfr10I RCCGGY 3 cut(s) 32, 143, 371
Cfr13I GGNCC 2 cut(s) 9, 518
CseI GACGC 3 cut(s) 37, 149, 232
Csp6I GTAC 2 cut(s) 507, 561
CviAII CATG 4 cut(s) 272, 437, 497, 523
CviQI GTAC 2 cut(s) 507, 561
DdeI CTNAG 4 cut(s) 531, 597, 678, 837
DpnI GATC 2 cut(s) 135, 166
DpnII GATC 2 cut(s) 133, 164
EaeI YGGCCR 2 cut(s) 433, 468
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 158
Eco24I GRGCYC 2 cut(s) 160, 496
Eco47I GGWCC 2 cut(s) 9, 518
Eco53kI GAGCTC 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 597
Eco91I GGTNACC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 740
EcoT38I GRGCYC 2 cut(s) 160, 496
FaeI CATG 4 cut(s) 275, 440, 500, 526
FaqI GGGAC 1 cut(s) 688
FatI CATG 4 cut(s) 271, 436, 496, 522
Fnu4HI GCNGC 5 cut(s) 74, 552, 693, 751, 813
FokI GGATG 2 cut(s) 443, 769
FriOI GRGCYC 2 cut(s) 160, 496
Fsp4HI GCNGC 5 cut(s) 74, 552, 693, 751, 813
FspBI CTAG 2 cut(s) 278, 564
GlaI GCGC 4 cut(s) 31, 92, 823, 842
GluI GCNGC 5 cut(s) 74, 552, 693, 751, 813
GsuI CTGGAG 1 cut(s) 837
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 3 cut(s) 42, 435, 470
HapII CCGG 5 cut(s) 25, 33, 144, 224, 372
HgaI GACGC 3 cut(s) 37, 149, 232
HhaI GCGC 4 cut(s) 32, 93, 824, 843
Hin1I GRCGYC 3 cut(s) 48, 141, 243
Hin1II CATG 4 cut(s) 275, 440, 500, 526
Hin6I GCGC 4 cut(s) 30, 91, 822, 841
HinP1I GCGC 4 cut(s) 30, 91, 822, 841
HincII GTYRAC 2 cut(s) 129, 149
HindII GTYRAC 2 cut(s) 129, 149
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 8 cut(s) 99, 125, 227, 406, 445, 651, 745, 785
HpaII CCGG 5 cut(s) 25, 33, 144, 224, 372
HphI GGTGA 1 cut(s) 641
Hpy166II GTNNAC 2 cut(s) 129, 149
Hpy188I TCNGA 7 cut(s) 80, 109, 164, 232, 285, 388, 667
Hpy188III TCNNGA 5 cut(s) 137, 184, 410, 487, 680
Hpy8I GTNNAC 2 cut(s) 129, 149
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 541, 604
HpyCH4III ACNGT 2 cut(s) 363, 809
HpyCH4IV ACGT 2 cut(s) 459, 699
HpyCH4V TGCA 4 cut(s) 271, 311, 695, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 295, 821, 828
HpyF3I CTNAG 4 cut(s) 531, 597, 678, 837
HpySE526I ACGT 2 cut(s) 459, 699
Hsp92I GRCGYC 3 cut(s) 48, 141, 243
Hsp92II CATG 4 cut(s) 275, 440, 500, 526
HspAI GCGC 4 cut(s) 30, 91, 822, 841
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
Kzo9I GATC 2 cut(s) 133, 164
LmnI GCTCC 4 cut(s) 27, 70, 81, 634
Lsp1109I GCAGC 3 cut(s) 563, 679, 799
LweI GCATC 1 cut(s) 298
MaeI CTAG 2 cut(s) 278, 564
MaeII ACGT 2 cut(s) 459, 699
MaeIII GTNAC 2 cut(s) 343, 357
MalI GATC 2 cut(s) 135, 166
MbiI CCGCTC 1 cut(s) 73
MboI GATC 2 cut(s) 133, 164
MboII GAAGA 3 cut(s) 214, 779, 794
MhlI GDGCHC 2 cut(s) 160, 496
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 5 cut(s) 103, 236, 422, 480, 726
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 3 cut(s) 93, 134, 236
MmeI TCCRAC 1 cut(s) 210
Mox20I TGGCCA 1 cut(s) 435
MroNI GCCGGC 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 736
MscI TGGCCA 1 cut(s) 435
MseI TTAA 1 cut(s) 425
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 554, 692
MspI CCGG 5 cut(s) 25, 33, 144, 224, 372
MspR9I CCNGG 1 cut(s) 742
MvaI CCWGG 1 cut(s) 742
MvnI CGCG 4 cut(s) 14, 21, 93, 750
MwoI GCNNNNNNNGC 4 cut(s) 27, 295, 821, 828
NaeI GCCGGC 1 cut(s) 373
NdeII GATC 2 cut(s) 133, 164
NgoMIV GCCGGC 1 cut(s) 371
NlaIII CATG 4 cut(s) 275, 440, 500, 526
NlaIV GGNNCC 5 cut(s) 77, 477, 520, 621, 687
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 357
NspV TTCGAA 1 cut(s) 730
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 565
PdiI GCCGGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 736
PfeI GAWTC 5 cut(s) 406, 445, 651, 745, 785
PfoI TCCNGGA 1 cut(s) 740
PkrI GCNGC 5 cut(s) 75, 553, 694, 752, 814
PleI GAGTC 3 cut(s) 93, 133, 235
PpsI GAGTC 3 cut(s) 93, 133, 235
Ppu21I YACGTR 1 cut(s) 700
PshAI GACNNNNGTC 1 cut(s) 144
Psp124BI GAGCTC 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 740
PspEI GGTNACC 1 cut(s) 343
PspGI CCWGG 1 cut(s) 740
PspN4I GGNNCC 5 cut(s) 77, 477, 520, 621, 687
PspPI GGNCC 2 cut(s) 9, 518
PsrI GAACNNNNNNTAC 2 cut(s) 562, 594
PvuII CAGCTG 2 cut(s) 554, 692
RsaI GTAC 2 cut(s) 508, 562
RsaNI GTAC 2 cut(s) 507, 561
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 5 cut(s) 74, 552, 693, 751, 813
Sau3AI GATC 2 cut(s) 133, 164
Sau96I GGNCC 2 cut(s) 9, 518
SchI GAGTC 3 cut(s) 93, 134, 236
ScrFI CCNGG 1 cut(s) 742
SduI GDGCHC 2 cut(s) 160, 496
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 848
SfuI TTCGAA 1 cut(s) 730
SinI GGWCC 2 cut(s) 9, 518
Sse9I AATT 5 cut(s) 103, 236, 422, 480, 726
SsiI CCGC 4 cut(s) 12, 68, 73, 750
SspMI CTAG 2 cut(s) 278, 564
SstI GAGCTC 1 cut(s) 160
StyD4I CCNGG 1 cut(s) 740
TaaI ACNGT 2 cut(s) 363, 809
TaiI ACGT 2 cut(s) 462, 702
TaqI TCGA 4 cut(s) 51, 409, 443, 730
TasI AATT 5 cut(s) 103, 236, 422, 480, 726
TatI WGTACW 1 cut(s) 506
TauI GCSGC 2 cut(s) 76, 753
TfiI GAWTC 5 cut(s) 406, 445, 651, 745, 785
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 357
TseI GCWGC 3 cut(s) 551, 692, 812
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 4 cut(s) 192, 382, 539, 623
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 2 cut(s) 9, 518
XapI RAATTY 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 736
XspI CTAG 2 cut(s) 278, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.