RchiOBHm_Chr3g0479811

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000940
Physical Location & Seq
Reverse (-)
25600162 .. 25602296
2135 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44487

Sequence Viewer

Length: 1125 bp
ATGGTGAAACGCGTATTGGGTTGTGCTACTAGTACTAGTAGCGGAGGAGATGAAACGCACGAAACTGATCACGCGACGCCGGCGGTGGCTGACCCTAGAGTACTCCGATCACGCTACCACACTGTCAAGACCATCCTCTACGATGAAAGAGAGGATCTCACAAGAGCAGATTCGGAGAAGTTCAATTCGATCATTAAGGAAGTGGAGAGCTTGCACCAGCTGGTAACAAAGCCAAGAGAGCAAGTCGCGGATGCGGAGGCGCTTTTGGATATTGCAAGCACCCTGATGACCTCTGTACTGGCACATAACAAGGAAGGAATTACAGCTTCGGGTTTCGTCAACTGTATTCTTAGTGACTTCAGGAAACCGAGTGGTAATGGCAGCAGCTCTATATCTTGGAGGGACATTGGGAATGCGGTATCGCATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATTGGGCCAATGAATGCTGTTGTGAAGCAACGAAAGGTTTATCAACGTCAAAAACGTGGGAATCCTACAGACAATGATACCCCTAAAGAGCTTGGTGATGCTGTTGTAGAAGAGGAAACAGAGACTGTTAAAAATGTGGCAACTATGTTTAATATTTTGAAGAAAAAGAGAAGGGTCAGGCTTGAATATCTAGTTTTGAATGGAAATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCGTTTCTTGTTAAAGATGGGCGAGTCGAAATAAAAGTGAACGAGGAAGGCCATCATCTAGTGTCGCCAAGGAATGCTCCTGCTGCCGAAGCAATTGCCTCAGGCGCTGTTACTTACTGCCACTTAATCTTCAGATTTGATTTCAAGGACTGGAAGTTAATGAAAGAAGTTGTTGGGAATGGGGAGGAGCTGATGCCACATAGGTCAGCTGACCATGTATCAAACAATTCGCAACTGGAAGAACCTCAAGCAACAACAGGTACAACACCAATTAGGAAGTTCTCAAGGAACAGAGGCTTGGTTATAAGGGAGCAGCACGTTGGGACTGATAGTAGCAGCAGATCAATTGCTGAAGACAGCGCTGAATGTGATTATGCTGCAAGCGCTTCTGCCACTCGTAAAGGGAAGCGTAAACTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

374

Amino Acids

41.47

Weight (kDa)

8.43

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 212 - 299 4e-22 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1007
AccII CGCG 3 cut(s) 12, 74, 248
AciI CCGC 5 cut(s) 42, 83, 248, 254, 416
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 3 cut(s) 343, 817, 1074
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 34, 102, 297, 964
AfeI AGCGCT 2 cut(s) 1063, 1087
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 5 cut(s) 184, 619, 644, 658, 847
AhlI ACTAGT 2 cut(s) 29, 35
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 983, 1015
AluBI AGCT 7 cut(s) 210, 220, 326, 387, 550, 892, 911
AluI AGCT 7 cut(s) 210, 220, 326, 387, 550, 892, 911
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 1 cut(s) 162
AlwNI CAGNNNCTG 2 cut(s) 584, 809
Aor51HI AGCGCT 2 cut(s) 1063, 1087
AoxI GGCC 2 cut(s) 464, 751
ApeKI GCWGC 7 cut(s) 381, 384, 450, 785, 1015, 1038, 1079
ApoI RAATTY 1 cut(s) 664
AspLEI GCGC 4 cut(s) 262, 809, 1064, 1088
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 16, 566
AxyI CCTNAGG 1 cut(s) 802
BbsI GAAGAC 1 cut(s) 1062
BbvI GCAGC 7 cut(s) 393, 396, 437, 772, 1027, 1050, 1066
BccI CCATC 3 cut(s) 140, 713, 762
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 575
BcuI ACTAGT 2 cut(s) 29, 35
BfaI CTAG 5 cut(s) 30, 36, 96, 650, 761
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 4 cut(s) 263, 810, 1065, 1089
BisI GCNGC 7 cut(s) 382, 385, 451, 786, 1016, 1039, 1080
BlsI GCNGC 7 cut(s) 383, 386, 452, 787, 1017, 1040, 1081
BmcAI AGTACT 2 cut(s) 34, 102
BmgT120I GGNCC 1 cut(s) 464
BmsI GCATC 4 cut(s) 241, 437, 547, 885
BpiI GAAGAC 1 cut(s) 1062
BplI GAGNNNNNCTC 4 cut(s) 141, 173, 427, 459
BpuEI CTTGAG 2 cut(s) 933, 970
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 770
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 3 cut(s) 303, 857, 942
Bse21I CCTNAGG 1 cut(s) 802
BseDI CCNNGG 1 cut(s) 770
BseGI GGATG 2 cut(s) 132, 256
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 3 cut(s) 303, 857, 942
BseRI GAGGAG 2 cut(s) 60, 902
BseXI GCAGC 7 cut(s) 393, 396, 437, 772, 1027, 1050, 1066
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 3 cut(s) 12, 74, 248
BshFI GGCC 2 cut(s) 466, 753
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 2 cut(s) 416, 1039
BsmAI GTCTC 1 cut(s) 575
BsmFI GGGAC 2 cut(s) 416, 1039
BsmI GAATGC 3 cut(s) 418, 478, 781
BsnI GGCC 2 cut(s) 466, 753
Bsp143I GATC 5 cut(s) 67, 107, 154, 189, 1043
BspACI CCGC 5 cut(s) 42, 83, 248, 254, 416
BspANI GGCC 2 cut(s) 466, 753
BspCNI CTCAG 1 cut(s) 815
BspFNI CGCG 3 cut(s) 12, 74, 248
BspPI GGATC 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 3 cut(s) 303, 857, 942
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 770
BssMI GATC 5 cut(s) 67, 107, 154, 189, 1043
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 770
Bst4CI ACNGT 4 cut(s) 124, 344, 586, 682
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 4 cut(s) 81, 212, 277, 1084
BstDEI CTNAG 2 cut(s) 350, 802
BstF5I GGATG 2 cut(s) 132, 256
BstFNI CGCG 3 cut(s) 12, 74, 248
BstH2I RGCGCY 4 cut(s) 263, 810, 1065, 1089
BstHHI GCGC 4 cut(s) 262, 809, 1064, 1088
BstKTI GATC 5 cut(s) 70, 110, 157, 192, 1046
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 5 cut(s) 67, 107, 154, 189, 1043
BstMWI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1085
BstNSI RCATGY 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 3 cut(s) 12, 74, 248
BstV1I GCAGC 7 cut(s) 393, 396, 437, 772, 1027, 1050, 1066
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
Bsu36I CCTNAGG 1 cut(s) 802
BsuRI GGCC 2 cut(s) 466, 753
BtsCI GGATG 2 cut(s) 132, 256
BtsIMutI CAGTG 2 cut(s) 120, 687
Cac8I GCNNGC 4 cut(s) 81, 212, 277, 1084
CaiI CAGNNNCTG 2 cut(s) 584, 809
CfoI GCGC 4 cut(s) 262, 809, 1064, 1088
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 85
Csp6I GTAC 4 cut(s) 33, 101, 296, 963
CviAII CATG 2 cut(s) 425, 917
CviQI GTAC 4 cut(s) 33, 101, 296, 963
DdeI CTNAG 2 cut(s) 350, 802
DpnI GATC 5 cut(s) 69, 109, 156, 191, 1045
DpnII GATC 5 cut(s) 67, 107, 154, 189, 1043
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 770
Eco47III AGCGCT 2 cut(s) 1063, 1087
Eco57I CTGAAG 3 cut(s) 343, 817, 1074
Eco81I CCTNAGG 1 cut(s) 802
EcoT14I CCWWGG 1 cut(s) 770
ErhI CCWWGG 1 cut(s) 770
FaeI CATG 2 cut(s) 428, 920
FaqI GGGAC 2 cut(s) 416, 1039
FatI CATG 2 cut(s) 424, 916
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 7 cut(s) 382, 385, 451, 786, 1016, 1039, 1080
FokI GGATG 2 cut(s) 119, 263
Fsp4HI GCNGC 7 cut(s) 382, 385, 451, 786, 1016, 1039, 1080
FspBI CTAG 5 cut(s) 30, 36, 96, 650, 761
GlaI GCGC 4 cut(s) 261, 808, 1063, 1087
GluI GCNGC 7 cut(s) 382, 385, 451, 786, 1016, 1039, 1080
HaeII RGCGCY 4 cut(s) 263, 810, 1065, 1089
HaeIII GGCC 2 cut(s) 466, 753
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 85
HhaI GCGC 4 cut(s) 262, 809, 1064, 1088
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 2 cut(s) 428, 920
Hin6I GCGC 4 cut(s) 260, 807, 1062, 1086
HinP1I GCGC 4 cut(s) 260, 807, 1062, 1086
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 3 cut(s) 170, 520, 726
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 16, 566
Hpy166II GTNNAC 3 cut(s) 340, 742, 1115
Hpy188I TCNGA 3 cut(s) 107, 175, 836
Hpy188III TCNNGA 3 cut(s) 127, 361, 433
Hpy8I GTNNAC 3 cut(s) 340, 742, 1115
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 3 cut(s) 308, 624, 743
HpyCH4III ACNGT 4 cut(s) 124, 344, 586, 682
HpyCH4IV ACGT 3 cut(s) 505, 514, 1020
HpyCH4V TGCA 4 cut(s) 214, 275, 453, 1082
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1085
HpyF3I CTNAG 2 cut(s) 350, 802
HpySE526I ACGT 3 cut(s) 505, 514, 1020
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 2 cut(s) 428, 920
HspAI GCGC 4 cut(s) 260, 807, 1062, 1086
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 107, 154, 189, 1043
LmnI GCTCC 3 cut(s) 784, 889, 1012
Lsp1109I GCAGC 7 cut(s) 393, 396, 437, 772, 1027, 1050, 1066
LweI GCATC 4 cut(s) 241, 437, 547, 885
MaeI CTAG 5 cut(s) 30, 36, 96, 650, 761
MaeII ACGT 3 cut(s) 505, 514, 1020
MaeIII GTNAC 3 cut(s) 223, 353, 811
MalI GATC 5 cut(s) 69, 109, 156, 191, 1045
MboI GATC 5 cut(s) 67, 107, 154, 189, 1043
MboII GAAGA 5 cut(s) 581, 631, 823, 953, 1067
MfeI CAATTG 2 cut(s) 795, 1047
MflI RGATCY 1 cut(s) 154
MluCI AATT 7 cut(s) 184, 318, 664, 795, 928, 972, 1047
MluI ACGCGT 1 cut(s) 10
MlyI GAGTC 1 cut(s) 735
MreI CGCCGGCG 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 220, 911
MspI CCGG 1 cut(s) 80
MunI CAATTG 2 cut(s) 795, 1047
Mva1269I GAATGC 3 cut(s) 418, 478, 781
MvnI CGCG 3 cut(s) 12, 74, 248
MwoI GCNNNNNNNGC 7 cut(s) 80, 238, 447, 785, 791, 806, 1085
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 5 cut(s) 67, 107, 154, 189, 1043
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 2 cut(s) 428, 920
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 511
PctI GAATGC 3 cut(s) 418, 478, 781
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 170, 520
PkrI GCNGC 7 cut(s) 383, 386, 452, 787, 1017, 1040, 1081
PleI GAGTC 1 cut(s) 734
PpsI GAGTC 1 cut(s) 734
PsiI TTATAA 1 cut(s) 1007
PspPI GGNCC 1 cut(s) 464
PstNI CAGNNNCTG 2 cut(s) 584, 809
PsuI RGATCY 1 cut(s) 154
PvuII CAGCTG 2 cut(s) 220, 911
RsaI GTAC 4 cut(s) 34, 102, 297, 964
RsaNI GTAC 4 cut(s) 33, 101, 296, 963
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
SatI GCNGC 7 cut(s) 382, 385, 451, 786, 1016, 1039, 1080
Sau3AI GATC 5 cut(s) 67, 107, 154, 189, 1043
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 2 cut(s) 34, 102
SchI GAGTC 1 cut(s) 735
SfaNI GCATC 4 cut(s) 241, 437, 547, 885
SfcI CTRYAG 1 cut(s) 525
SgrAI CRCCGGYG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 948, 985
SmoI CTYRAG 2 cut(s) 948, 985
SpeI ACTAGT 2 cut(s) 29, 35
Sse9I AATT 7 cut(s) 184, 318, 664, 795, 928, 972, 1047
SsiI CCGC 5 cut(s) 42, 83, 248, 254, 416
SspI AATATT 1 cut(s) 613
SspMI CTAG 5 cut(s) 30, 36, 96, 650, 761
StyI CCWWGG 1 cut(s) 770
TaaI ACNGT 4 cut(s) 124, 344, 586, 682
TaiI ACGT 3 cut(s) 508, 517, 1023
TaqI TCGA 3 cut(s) 188, 445, 729
TasI AATT 7 cut(s) 184, 318, 664, 795, 928, 972, 1047
TatI WGTACW 3 cut(s) 32, 100, 295
TfiI GAWTC 2 cut(s) 170, 520
Tru1I TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
Tru9I TTAA 6 cut(s) 195, 588, 609, 714, 827, 860
TscAI CASTG 2 cut(s) 127, 687
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 7 cut(s) 381, 384, 450, 785, 1015, 1038, 1079
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 4 cut(s) 66, 159, 485, 878
TspRI CASTG 2 cut(s) 127, 687
XapI RAATTY 1 cut(s) 664
XceI RCATGY 1 cut(s) 428
XspI CTAG 5 cut(s) 30, 36, 96, 650, 761
ZrmI AGTACT 2 cut(s) 34, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.