FvH4_6g22450

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000940
Physical Location & Seq
Reverse (-)
16129906 .. 16132125
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22450.t2

Sequence Viewer

Length: 1119 bp
ATGGTGAAACGCGAGTTGGGTTGTGCCACTGGTAGCGGTGGAGATGAAACGGACGAACCTGATCACCCCGCGGCGGTGCCGGTGACTGACCCTAGAGCACTCCGTTCACGCTACCACACCGTCAAGACCATCCTCTATGATGAAAGAGAAGATCTCACAAGAGCAGACTCGAAGAAGTTCGATTCCATCATTGAGCAAGTGGAGAGCTTGCACCAGCTGGTAAGAAATCCAAGAGAGCAAGTAGCGGATGCGGAGGCGCTTTTGGACATTGCAAGCACCTTGATGACCTCTGTAAGGGCACATAACAAGGAAGGGATTACAGCTTCGGGTTTCATCAACTGTATTCTTACTGACTTCAGGAAACCAAGTGGTAATGGCAGCAGCTCAATATATTGGAGGGACATTGGGAATGCGGTGTCACATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATCGGGCCTATGAATGCTGAAGTGAAGCGAAGAAAGCCTTATCAACGTCACAGACGTGGGAATCCTACAGACAATGATACCCCTCAAGAGCTTGGTGATACTGTTGAAGAAGAGGAAACAGAGACTGTTAAAAACGTGTCCACTATGTTTAATATCTTGAGGAAAGAGAGAAGGGTCAGGCTTGAAAATCTAGTGTTAAATGGAAATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCATTTCTTGTTAAAGATGGGCGTGTTGAAATGAAAGTGAATGAGGAAGGCCATCATCTAGTGTCGCCAAGAAATGCTCCTGCTGCCAAAGCAATTGCCTCAGGGGATGTTGCTTACTTCCACTTGATCTTCAGATATGATTTCAAGGACTGGAAGTTAATGAAGGAGGTTGTTGGGACCGGAGAGGAGCTGATGCCACATAGGTTTCCAGCAGACATATCAAACAACTCGCAACCAGAAGAAACTCCTGCAACAATAGGTACAACACCAATTAGGAAGTTTTCGAGGAACAGAGGCTTGGTTACACGGGAGCAGCCCGTTGGAACTGATAGTAGTATATCAATTGCTGAAGACAGCGCCGAATGTGATTATGCTGCAAGAGCCGCACCCACTCGTAAAGGGAAGCGTAAACTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

373

Amino Acids

41.67

Weight (kDa)

6.82

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 211 - 287 1.5e-19 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccII CGCG 2 cut(s) 12, 71
AciI CCGC 8 cut(s) 36, 69, 71, 74, 245, 251, 413, 1083
AcsI RAATTY 1 cut(s) 661
AcuI CTGAAG 4 cut(s) 340, 495, 814, 1068
AfaI GTAC 1 cut(s) 961
AfiI CCNNNNNNNGG 2 cut(s) 73, 294
AflIII ACRYGT 2 cut(s) 421, 591
AgsI TTSAA 4 cut(s) 563, 641, 728, 844
AjiI CACGTC 1 cut(s) 512
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 980, 1012
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 6 cut(s) 207, 217, 323, 384, 547, 889
AluI AGCT 6 cut(s) 207, 217, 323, 384, 547, 889
Alw21I GWGCWC 1 cut(s) 100
Alw26I GTCTC 1 cut(s) 572
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 2 cut(s) 461, 748
ApeKI GCWGC 6 cut(s) 378, 381, 447, 782, 1012, 1073
ApoI RAATTY 1 cut(s) 661
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 2 cut(s) 259, 1058
AspS9I GGNCC 2 cut(s) 461, 876
AsuHPI GGTGA 4 cut(s) 16, 56, 94, 563
AvaII GGWCC 1 cut(s) 876
AxyI CCTNAGG 1 cut(s) 799
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 76
BbsI GAAGAC 1 cut(s) 1056
Bbv12I GWGCWC 1 cut(s) 100
BbvI GCAGC 6 cut(s) 390, 393, 434, 769, 1024, 1060
BccI CCATC 4 cut(s) 137, 194, 710, 759
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 572
BfaI CTAG 3 cut(s) 93, 647, 758
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 2 cut(s) 260, 1059
BglII AGATCT 1 cut(s) 151
BisI GCNGC 8 cut(s) 72, 379, 382, 448, 783, 1013, 1074, 1083
BlsI GCNGC 8 cut(s) 73, 380, 383, 449, 784, 1014, 1075, 1084
Bme18I GGWCC 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 512
BmgT120I GGNCC 2 cut(s) 461, 876
BmiI GGNNCC 2 cut(s) 78, 877
BmsI GCATC 3 cut(s) 238, 434, 882
BpiI GAAGAC 1 cut(s) 1056
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 424, 456
BpuEI CTTGAG 2 cut(s) 525, 634
BsaJI CCNNGG 1 cut(s) 69
BsaWI WCCGGW 1 cut(s) 878
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 294
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 2 cut(s) 34, 854
Bse21I CCTNAGG 1 cut(s) 799
Bse3DI GCAATG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 3 cut(s) 129, 253, 811
BseLI CCNNNNNNNGG 2 cut(s) 73, 294
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 813
BseNI ACTGG 2 cut(s) 34, 854
BseRI GAGGAG 1 cut(s) 899
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 6 cut(s) 390, 393, 434, 769, 1024, 1060
BsgI GTGCAG 1 cut(s) 433
Bsh1236I CGCG 2 cut(s) 12, 71
BshFI GGCC 2 cut(s) 463, 750
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 100
BsiSI CCGG 2 cut(s) 80, 879
BslFI GGGAC 2 cut(s) 413, 889
BslI CCNNNNNNNGG 2 cut(s) 73, 294
BsmAI GTCTC 1 cut(s) 572
BsmFI GGGAC 2 cut(s) 413, 889
BsmI GAATGC 2 cut(s) 415, 475
BsnI GGCC 2 cut(s) 463, 750
Bsp1286I GDGCHC 2 cut(s) 100, 301
Bsp143I GATC 4 cut(s) 61, 151, 456, 825
BspACI CCGC 8 cut(s) 36, 69, 71, 74, 245, 251, 413, 1083
BspANI GGCC 2 cut(s) 463, 750
BspCNI CTCAG 1 cut(s) 812
BspFNI CGCG 2 cut(s) 12, 71
BspLI GGNNCC 2 cut(s) 78, 877
BspT107I GGYRCC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 2 cut(s) 34, 854
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 4 cut(s) 61, 151, 456, 825
Bst4CI ACNGT 5 cut(s) 121, 341, 559, 583, 679
Bst6I CTCTTC 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 209, 274
BstDEI CTNAG 1 cut(s) 799
BstDSI CCRYGG 1 cut(s) 69
BstENI CCTNNNNNAGG 1 cut(s) 292
BstF5I GGATG 3 cut(s) 129, 253, 811
BstFNI CGCG 2 cut(s) 12, 71
BstH2I RGCGCY 2 cut(s) 260, 1059
BstHHI GCGC 2 cut(s) 259, 1058
BstKTI GATC 4 cut(s) 64, 154, 459, 828
BstMAI GTCTC 1 cut(s) 572
BstMBI GATC 4 cut(s) 61, 151, 456, 825
BstMWI GCNNNNNNNGC 6 cut(s) 444, 490, 782, 788, 1079, 1082
BstNSI RCATGY 1 cut(s) 425
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 2 cut(s) 12, 71
BstV1I GCAGC 6 cut(s) 390, 393, 434, 769, 1024, 1060
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 799
BsuRI GGCC 2 cut(s) 463, 750
BtgI CCRYGG 1 cut(s) 69
BtrI CACGTC 1 cut(s) 512
BtsCI GGATG 3 cut(s) 129, 253, 811
BtsIMutI CAGTG 2 cut(s) 27, 684
Cac8I GCNNGC 2 cut(s) 209, 274
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 2 cut(s) 259, 1058
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 461, 876
Cfr42I CCGCGG 1 cut(s) 72
Csp6I GTAC 1 cut(s) 960
CviAII CATG 1 cut(s) 422
CviQI GTAC 1 cut(s) 960
DdeI CTNAG 1 cut(s) 799
DpnI GATC 4 cut(s) 63, 153, 458, 827
DpnII GATC 4 cut(s) 61, 151, 456, 825
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 876
Eco57I CTGAAG 4 cut(s) 340, 495, 814, 1068
Eco81I CCTNAGG 1 cut(s) 799
EcoNI CCTNNNNNAGG 1 cut(s) 292
FaeI CATG 1 cut(s) 425
FalI AAGNNNNNCTT 2 cut(s) 478, 510
FaqI GGGAC 2 cut(s) 413, 889
FatI CATG 1 cut(s) 421
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 8 cut(s) 72, 379, 382, 448, 783, 1013, 1074, 1083
FokI GGATG 3 cut(s) 116, 260, 818
Fsp4HI GCNGC 8 cut(s) 72, 379, 382, 448, 783, 1013, 1074, 1083
FspBI CTAG 3 cut(s) 93, 647, 758
GlaI GCGC 2 cut(s) 258, 1057
GluI GCNGC 8 cut(s) 72, 379, 382, 448, 783, 1013, 1074, 1083
HaeII RGCGCY 2 cut(s) 260, 1059
HaeIII GGCC 2 cut(s) 463, 750
HapII CCGG 2 cut(s) 80, 879
HhaI GCGC 2 cut(s) 259, 1058
Hin1II CATG 1 cut(s) 425
Hin6I GCGC 2 cut(s) 257, 1056
HinP1I GCGC 2 cut(s) 257, 1056
HinfI GANTC 3 cut(s) 167, 182, 517
HpaII CCGG 2 cut(s) 80, 879
HphI GGTGA 4 cut(s) 16, 56, 94, 563
Hpy166II GTNNAC 3 cut(s) 107, 597, 1109
Hpy188I TCNGA 1 cut(s) 833
Hpy188III TCNNGA 5 cut(s) 124, 358, 430, 542, 613
Hpy8I GTNNAC 3 cut(s) 107, 597, 1109
HpyAV CCTTC 4 cut(s) 305, 621, 740, 856
HpyCH4III ACNGT 5 cut(s) 121, 341, 559, 583, 679
HpyCH4IV ACGT 3 cut(s) 502, 511, 591
HpyCH4V TGCA 5 cut(s) 211, 272, 450, 950, 1076
HpyF10VI GCNNNNNNNGC 6 cut(s) 444, 490, 782, 788, 1079, 1082
HpyF3I CTNAG 1 cut(s) 799
HpySE526I ACGT 3 cut(s) 502, 511, 591
Hsp92II CATG 1 cut(s) 425
HspAI GCGC 2 cut(s) 257, 1056
Ksp22I TGATCA 1 cut(s) 61
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 4 cut(s) 61, 151, 456, 825
LmnI GCTCC 3 cut(s) 781, 886, 1009
Lsp1109I GCAGC 6 cut(s) 390, 393, 434, 769, 1024, 1060
LweI GCATC 3 cut(s) 238, 434, 882
MaeI CTAG 3 cut(s) 93, 647, 758
MaeII ACGT 3 cut(s) 502, 511, 591
MaeIII GTNAC 4 cut(s) 82, 417, 503, 1000
MalI GATC 4 cut(s) 63, 153, 458, 827
MboI GATC 4 cut(s) 61, 151, 456, 825
MboII GAAGA 8 cut(s) 161, 184, 498, 575, 578, 820, 950, 1061
MfeI CAATTG 2 cut(s) 792, 1041
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 100, 301
MluCI AATT 4 cut(s) 661, 792, 969, 1041
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 1000
MroXI GAANNNNTTC 1 cut(s) 176
MseI TTAA 5 cut(s) 585, 606, 653, 711, 857
MslI CAYNNNNRTG 2 cut(s) 281, 510
MspA1I CMGCKG 2 cut(s) 71, 217
MspI CCGG 2 cut(s) 80, 879
MunI CAATTG 2 cut(s) 792, 1041
Mva1269I GAATGC 2 cut(s) 415, 475
MvnI CGCG 2 cut(s) 12, 71
MwoI GCNNNNNNNGC 6 cut(s) 444, 490, 782, 788, 1079, 1082
NdeII GATC 4 cut(s) 61, 151, 456, 825
NlaIII CATG 1 cut(s) 425
NlaIV GGNNCC 2 cut(s) 78, 877
NmuCI GTSAC 3 cut(s) 82, 417, 503
NspI RCATGY 1 cut(s) 425
OliI CACNNNNGTG 1 cut(s) 510
PciI ACATGT 1 cut(s) 421
PcsI WCGNNNNNNNCGW 1 cut(s) 508
PctI GAATGC 2 cut(s) 415, 475
PdmI GAANNNNTTC 1 cut(s) 176
PfeI GAWTC 2 cut(s) 182, 517
PkrI GCNGC 8 cut(s) 73, 380, 383, 449, 784, 1014, 1075, 1084
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PscI ACATGT 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 78, 877
PspPI GGNCC 2 cut(s) 461, 876
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 961
RsaNI GTAC 1 cut(s) 960
RseI CAYNNNNRTG 2 cut(s) 281, 510
SacII CCGCGG 1 cut(s) 72
SaqAI TTAA 5 cut(s) 585, 606, 653, 711, 857
SatI GCNGC 8 cut(s) 72, 379, 382, 448, 783, 1013, 1074, 1083
Sau3AI GATC 4 cut(s) 61, 151, 456, 825
Sau96I GGNCC 2 cut(s) 461, 876
SchI GAGTC 1 cut(s) 161
SduI GDGCHC 2 cut(s) 100, 301
SfaNI GCATC 3 cut(s) 238, 434, 882
SfcI CTRYAG 1 cut(s) 522
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SinI GGWCC 1 cut(s) 876
SmiMI CAYNNNNRTG 2 cut(s) 281, 510
SmlI CTYRAG 2 cut(s) 540, 613
SmoI CTYRAG 2 cut(s) 540, 613
Sse9I AATT 4 cut(s) 661, 792, 969, 1041
SsiI CCGC 8 cut(s) 36, 69, 71, 74, 245, 251, 413, 1083
SspMI CTAG 3 cut(s) 93, 647, 758
TaaI ACNGT 5 cut(s) 121, 341, 559, 583, 679
TaiI ACGT 3 cut(s) 505, 514, 594
TaqI TCGA 4 cut(s) 170, 180, 442, 983
TasI AATT 4 cut(s) 661, 792, 969, 1041
TauI GCSGC 2 cut(s) 74, 1085
TfiI GAWTC 2 cut(s) 182, 517
Tru1I TTAA 5 cut(s) 585, 606, 653, 711, 857
Tru9I TTAA 5 cut(s) 585, 606, 653, 711, 857
TscAI CASTG 2 cut(s) 34, 684
TseFI GTSAC 3 cut(s) 82, 417, 503
TseI GCWGC 6 cut(s) 378, 381, 447, 782, 1012, 1073
Tsp45I GTSAC 3 cut(s) 82, 417, 503
TspDTI ATGAA 6 cut(s) 60, 156, 322, 482, 746, 875
TspGWI ACGGA 2 cut(s) 65, 92
TspRI CASTG 2 cut(s) 34, 684
VpaK11BI GGWCC 1 cut(s) 876
XagI CCTNNNNNAGG 1 cut(s) 292
XapI RAATTY 1 cut(s) 661
XceI RCATGY 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 176
XspI CTAG 3 cut(s) 93, 647, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.