Rw0G002240

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00072
OG0000940
Physical Location & Seq
Forward (+)
97337 .. 99270
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002240.1

Sequence Viewer

Length: 1026 bp
ATGGTGAAACGCGTATTGGGTTGTGCTACTAGTACTAGTAGAGGAGGAGATGAAACGCACGAAACTGATCACGCGACGCCGGCGCTGGCTGACCCTAGAGTACTCCGATCACGCTACCATCCTCTACTAGAGGATCTCACAAGAGCAGATTCGGAGAAGTTCAATTCGAACATTAAGGAAGTGGAGAGCTTGCACCAGCTGGAGCAAGTCGCGGATGCGGAGGCGCTTTTGGACATTGCAAGCACCCTAATAACCTCTGTACGGGCACATAACAAGGAAGGAATTACAGCTTCGGGTTTCGTCAACTGTATTCTTAGTGACTTCAGGAAACCGAGTGGTAATGGCAGCAGCTCTATATCTTTGAGGGACATTGGGAATGCGGTATCGCATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATTGGGCCAATGAATGCCGTTGTGAAGCAACGAAAGGTTTATCAACGTCAAAAACGTGGGAATCCTACAGACAATGATACCCCTAAAGAGCTTGGTGATGCTGTTGTAGAAGAGGAAACAGAGACTGTTAAAAATGTGGCAACTATGTTTAATATTTTGAAGAAAAAGAGAAGGGTCAGGCTTGAATATCTAGTTTTGAATGGAAATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCGTTTCTTGTTAAAGATGGGCGATTTTTCTCTGTGTTTGGTATGTCAGGGCCAAGGAATGCTCCTGCTGCTGAAGCAATTGCCTCAGGCGCTGTTACTTACTTTGTTGGGAATGGGGAGGAGCTGATGCCTCATAGGTTTCCAGCTGACGATGTATCAAACAATTCGCAACTGGAAGAACCTCAAGCAACAACAGGTACAACACCAATTAGGAAGTTCTCAAGGAACAGAGGCTTGGTTATAAGGGAGCAGCCCGTTGGGACTGATAGTAGCAGCAGATCAATTGCTGAAGACAGCGCTGAATGTGATTATGCTGCAAGCGCTTCTGCCACTCGTAAAGGGAAGCGTAAACTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

341

Amino Acids

37.32

Weight (kDa)

8.54

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 200 - 231 3.8e-10 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 908
AccII CGCG 3 cut(s) 12, 74, 212
AciI CCGC 3 cut(s) 212, 218, 380
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 628
AcuI CTGAAG 3 cut(s) 307, 759, 975
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 4 cut(s) 34, 102, 261, 865
AfeI AGCGCT 2 cut(s) 964, 988
AfiI CCNNNNNNNGG 1 cut(s) 261
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 4 cut(s) 163, 583, 608, 622
AhlI ACTAGT 2 cut(s) 29, 35
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 884, 916
AluBI AGCT 7 cut(s) 189, 199, 290, 351, 514, 790, 812
AluI AGCT 7 cut(s) 189, 199, 290, 351, 514, 790, 812
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 1 cut(s) 141
AlwNI CAGNNNCTG 2 cut(s) 548, 758
Aor51HI AGCGCT 2 cut(s) 964, 988
AoxI GGCC 2 cut(s) 428, 716
ApeKI GCWGC 7 cut(s) 345, 348, 414, 734, 916, 939, 980
ApoI RAATTY 1 cut(s) 628
AspLEI GCGC 5 cut(s) 85, 226, 758, 965, 989
AspS9I GGNCC 2 cut(s) 428, 716
AsuHPI GGTGA 2 cut(s) 16, 530
AsuII TTCGAA 1 cut(s) 167
AxyI CCTNAGG 1 cut(s) 751
BaeGI GKGCMC 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 963
BbvI GCAGC 7 cut(s) 357, 360, 401, 721, 928, 951, 967
BccI CCATC 2 cut(s) 126, 677
BceAI ACGGC 1 cut(s) 425
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 539
BcuI ACTAGT 2 cut(s) 29, 35
BfaI CTAG 5 cut(s) 30, 36, 96, 128, 614
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 5 cut(s) 86, 227, 759, 966, 990
BisI GCNGC 7 cut(s) 346, 349, 415, 735, 917, 940, 981
BlsI GCNGC 7 cut(s) 347, 350, 416, 736, 918, 941, 982
BmcAI AGTACT 2 cut(s) 34, 102
BmgT120I GGNCC 2 cut(s) 428, 716
BmsI GCATC 4 cut(s) 205, 401, 511, 783
BpiI GAAGAC 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 391, 423
BpmI CTGGAG 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 2 cut(s) 834, 871
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 719
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 843
Bse21I CCTNAGG 1 cut(s) 751
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 2 cut(s) 118, 220
BseLI CCNNNNNNNGG 1 cut(s) 261
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 1 cut(s) 843
BseRI GAGGAG 3 cut(s) 57, 60, 800
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 7 cut(s) 357, 360, 401, 721, 928, 951, 967
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 3 cut(s) 12, 74, 212
BshFI GGCC 2 cut(s) 430, 718
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 2 cut(s) 380, 940
BslI CCNNNNNNNGG 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 539
BsmFI GGGAC 2 cut(s) 380, 940
BsmI GAATGC 3 cut(s) 382, 442, 730
BsnI GGCC 2 cut(s) 430, 718
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 4 cut(s) 67, 107, 133, 944
BspACI CCGC 3 cut(s) 212, 218, 380
BspANI GGCC 2 cut(s) 430, 718
BspCNI CTCAG 1 cut(s) 764
BspFNI CGCG 3 cut(s) 12, 74, 212
BspPI GGATC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 843
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 719
BssMI GATC 4 cut(s) 67, 107, 133, 944
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 719
Bst4CI ACNGT 3 cut(s) 308, 550, 646
Bst6I CTCTTC 1 cut(s) 528
BstACI GRCGYC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 5 cut(s) 81, 87, 191, 241, 985
BstDEI CTNAG 2 cut(s) 314, 751
BstF5I GGATG 2 cut(s) 118, 220
BstFNI CGCG 3 cut(s) 12, 74, 212
BstH2I RGCGCY 5 cut(s) 86, 227, 759, 966, 990
BstHHI GCGC 5 cut(s) 85, 226, 758, 965, 989
BstKTI GATC 4 cut(s) 70, 110, 136, 947
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 4 cut(s) 67, 107, 133, 944
BstMWI GCNNNNNNNGC 6 cut(s) 80, 411, 734, 740, 755, 986
BstNSI RCATGY 1 cut(s) 392
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 268
BstUI CGCG 3 cut(s) 12, 74, 212
BstV1I GCAGC 7 cut(s) 357, 360, 401, 721, 928, 951, 967
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Bsu36I CCTNAGG 1 cut(s) 751
BsuRI GGCC 2 cut(s) 430, 718
BtsCI GGATG 2 cut(s) 118, 220
BtsIMutI CAGTG 1 cut(s) 651
Cac8I GCNNGC 5 cut(s) 81, 87, 191, 241, 985
CaiI CAGNNNCTG 2 cut(s) 548, 758
CfoI GCGC 5 cut(s) 85, 226, 758, 965, 989
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 428, 716
CseI GACGC 1 cut(s) 85
Csp6I GTAC 4 cut(s) 33, 101, 260, 864
CviAII CATG 1 cut(s) 389
CviQI GTAC 4 cut(s) 33, 101, 260, 864
DdeI CTNAG 2 cut(s) 314, 751
DpnI GATC 4 cut(s) 69, 109, 135, 946
DpnII GATC 4 cut(s) 67, 107, 133, 944
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 719
Eco47III AGCGCT 2 cut(s) 964, 988
Eco57I CTGAAG 3 cut(s) 307, 759, 975
Eco81I CCTNAGG 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 719
FaeI CATG 1 cut(s) 392
FaqI GGGAC 2 cut(s) 380, 940
FatI CATG 1 cut(s) 388
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 7 cut(s) 346, 349, 415, 735, 917, 940, 981
FokI GGATG 2 cut(s) 105, 227
Fsp4HI GCNGC 7 cut(s) 346, 349, 415, 735, 917, 940, 981
FspBI CTAG 5 cut(s) 30, 36, 96, 128, 614
GlaI GCGC 5 cut(s) 84, 225, 757, 964, 988
GluI GCNGC 7 cut(s) 346, 349, 415, 735, 917, 940, 981
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 5 cut(s) 86, 227, 759, 966, 990
HaeIII GGCC 2 cut(s) 430, 718
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 85
HhaI GCGC 5 cut(s) 85, 226, 758, 965, 989
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 392
Hin6I GCGC 5 cut(s) 83, 224, 756, 963, 987
HinP1I GCGC 5 cut(s) 83, 224, 756, 963, 987
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 2 cut(s) 149, 484
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 16, 530
Hpy166II GTNNAC 2 cut(s) 304, 1016
Hpy188I TCNGA 2 cut(s) 107, 154
Hpy188III TCNNGA 2 cut(s) 325, 397
Hpy8I GTNNAC 2 cut(s) 304, 1016
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 2 cut(s) 272, 588
HpyCH4III ACNGT 3 cut(s) 308, 550, 646
HpyCH4IV ACGT 2 cut(s) 469, 478
HpyCH4V TGCA 4 cut(s) 193, 239, 417, 983
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 411, 734, 740, 755, 986
HpyF3I CTNAG 2 cut(s) 314, 751
HpySE526I ACGT 2 cut(s) 469, 478
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 392
HspAI GCGC 5 cut(s) 83, 224, 756, 963, 987
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 4 cut(s) 67, 107, 133, 944
LmnI GCTCC 4 cut(s) 202, 733, 787, 913
Lsp1109I GCAGC 7 cut(s) 357, 360, 401, 721, 928, 951, 967
LweI GCATC 4 cut(s) 205, 401, 511, 783
MaeI CTAG 5 cut(s) 30, 36, 96, 128, 614
MaeII ACGT 2 cut(s) 469, 478
MaeIII GTNAC 2 cut(s) 317, 760
MalI GATC 4 cut(s) 69, 109, 135, 946
MboI GATC 4 cut(s) 67, 107, 133, 944
MboII GAAGA 4 cut(s) 545, 595, 854, 968
MfeI CAATTG 2 cut(s) 744, 948
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 7 cut(s) 163, 282, 628, 744, 829, 873, 948
MluI ACGCGT 1 cut(s) 10
MreI CGCCGGCG 1 cut(s) 79
MroNI GCCGGC 1 cut(s) 79
MseI TTAA 4 cut(s) 174, 552, 573, 678
MspA1I CMGCKG 2 cut(s) 199, 812
MspI CCGG 1 cut(s) 80
MunI CAATTG 2 cut(s) 744, 948
Mva1269I GAATGC 3 cut(s) 382, 442, 730
MvnI CGCG 3 cut(s) 12, 74, 212
MwoI GCNNNNNNNGC 6 cut(s) 80, 411, 734, 740, 755, 986
NaeI GCCGGC 1 cut(s) 81
NdeII GATC 4 cut(s) 67, 107, 133, 944
NgoMIV GCCGGC 1 cut(s) 79
NlaIII CATG 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 317
NspI RCATGY 1 cut(s) 392
NspV TTCGAA 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 475
PctI GAATGC 3 cut(s) 382, 442, 730
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 149, 484
PkrI GCNGC 7 cut(s) 347, 350, 416, 736, 918, 941, 982
PsiI TTATAA 1 cut(s) 908
PspPI GGNCC 2 cut(s) 428, 716
PstNI CAGNNNCTG 2 cut(s) 548, 758
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 2 cut(s) 199, 812
RsaI GTAC 4 cut(s) 34, 102, 261, 865
RsaNI GTAC 4 cut(s) 33, 101, 260, 864
SaqAI TTAA 4 cut(s) 174, 552, 573, 678
SatI GCNGC 7 cut(s) 346, 349, 415, 735, 917, 940, 981
Sau3AI GATC 4 cut(s) 67, 107, 133, 944
Sau96I GGNCC 2 cut(s) 428, 716
ScaI AGTACT 2 cut(s) 34, 102
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 4 cut(s) 205, 401, 511, 783
SfcI CTRYAG 1 cut(s) 489
SfuI TTCGAA 1 cut(s) 167
SgrAI CRCCGGYG 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 849, 886
SmoI CTYRAG 2 cut(s) 849, 886
SpeI ACTAGT 2 cut(s) 29, 35
Sse9I AATT 7 cut(s) 163, 282, 628, 744, 829, 873, 948
SsiI CCGC 3 cut(s) 212, 218, 380
SspI AATATT 1 cut(s) 577
SspMI CTAG 5 cut(s) 30, 36, 96, 128, 614
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 3 cut(s) 308, 550, 646
TaiI ACGT 2 cut(s) 472, 481
TaqI TCGA 2 cut(s) 167, 409
TasI AATT 7 cut(s) 163, 282, 628, 744, 829, 873, 948
TatI WGTACW 2 cut(s) 32, 100
TfiI GAWTC 2 cut(s) 149, 484
Tru1I TTAA 4 cut(s) 174, 552, 573, 678
Tru9I TTAA 4 cut(s) 174, 552, 573, 678
TscAI CASTG 1 cut(s) 651
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 7 cut(s) 345, 348, 414, 734, 916, 939, 980
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 66, 449
TspRI CASTG 1 cut(s) 651
XapI RAATTY 1 cut(s) 628
XceI RCATGY 1 cut(s) 392
XspI CTAG 5 cut(s) 30, 36, 96, 128, 614
ZrmI AGTACT 2 cut(s) 34, 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.