AT1G51130

Non-structural maintenance of chromosomes element 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000940
Physical Location & Seq
Reverse (-)
18939194 .. 18941719
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G51130.1

Sequence Viewer

Length: 1212 bp
ATGAGGAAGACGGTGAAGCGAGAATCGGAAGCCACCGGCGGGAAAAGAGAAGCCGACGACGAGCCGGAGAAGTTGAGGTCTGTGAAGAAAGAGAAACAGAGAAAAACCGAAGCTGACTCCGTACGACCCGACGAGCCTCCTCCGCCTCAGGAGGAAGAACAAGGAATATCTGATCGGAGAATTCTCAGATCCAAGTATCTCTCTCTTCAAAACGAAATCAACGATTGTAAAGATGATTTGATGAAGATTGATTCTGACAAATTCTCCAGAATTATTAATGCAGTTGAAAACTTGCACCAACAAGTTCGGAAGCCTAGGGAACAAATTGCAGACGCAGAGGCACTTCTGGATATAGCAAACACTTTAATGTCATCGGTGAAGTCTCAGTCTGCTCATGGGGTTTCACCGGCTGAGTTTGTTAATGCTTTGATCAGTGGGTTTGGTCAAGGTTCTTTGGGGATTGATACTGATGAAACTGCTCAAGTATCTTTAAAGTGGAAGGATCTTGGGTTTGCTGTTTGTTCTACTGTTTTAGTATCATGTGGTTGTTCTACAATGTTGGGACCTATGGATACTGAACTGAAGCAAAGGAAAAGGGCACCCAACAGAAAACGTACAAAGCCTGGTGAAGGTGTTCGTCCAGATGAGGTAGATGATTCACAATCAGAAGAAAAAACTGATACAGACAAGAATATGACAATAATGTTTAACATCTTGGGGAAAAAGAAGCGCGTGCAGCTTGAGAATTTGGTGCTCAACAGGCGGTCATTTGCACAGACTGTAGAGAATTTGTTTGCTCTATCTTTCTTGGCCAAAGATGGACGAGTTGAGATCATTGTCGACAAAAGTGGTTCTCATTTCGCCATGCCGAGAAATGCACCTGATGCAAACGTGGTGATGTCGGGTGAAGTCATTTATAACCATTTTGTGTTTAGATTAGATTTCAAAGATTGGAAGTTGATGTCTGAAATGGTGCCACTGGGCGAGGAGCTAATGCCACACAGACAAACTGCAGTTGCTTCATCTTCTTGTCCCGCTGCATCAGCCCCTGCATCAGCAGACTTCACACAAGATACTCAGACAACACCAATAAGAAAACTATCGAGAAACCGAGGGTTGGTTGTACAAGAAGAAACTGTTGTAGAAGATACTCCTGACAAAGAGGGCGATGGGACTCGAAGGAGATGTAAGCGGAGACTCACCTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

403

Amino Acids

45.08

Weight (kDa)

6.99

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 245 - 332 2e-21 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 918
AccB1I GGYRCC 2 cut(s) 598, 973
AccI GTMKAC 1 cut(s) 840
AccII CGCG 1 cut(s) 732
AciI CCGC 5 cut(s) 39, 143, 763, 1035, 1192
AclWI GGATC 2 cut(s) 183, 510
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 4 cut(s) 180, 260, 745, 787
AcuI CTGAAG 1 cut(s) 602
AfaI GTAC 3 cut(s) 123, 616, 1125
AfiI CCNNNNNNNGG 3 cut(s) 39, 629, 1117
AgsI TTSAA 3 cut(s) 209, 287, 946
AjnI CCWGG 1 cut(s) 622
AluBI AGCT 3 cut(s) 113, 739, 991
AluI AGCT 3 cut(s) 113, 739, 991
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 2 cut(s) 387, 1189
AlwI GGATC 2 cut(s) 183, 510
AlwNI CAGNNNCTG 1 cut(s) 1049
AoxI GGCC 1 cut(s) 810
ApeKI GCWGC 2 cut(s) 736, 1037
ApoI RAATTY 4 cut(s) 180, 260, 745, 787
AseI ATTAAT 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 633
AspA2I CCTAGG 1 cut(s) 314
AspLEI GCGC 1 cut(s) 732
AspS9I GGNCC 1 cut(s) 563
AsuHPI GGTGA 7 cut(s) 25, 388, 396, 638, 907, 917, 1192
AvaII GGWCC 1 cut(s) 563
AvrII CCTAGG 1 cut(s) 314
AxyI CCTNAGG 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 1 cut(s) 812
BanI GGYRCC 2 cut(s) 598, 973
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 756
BbvI GCAGC 2 cut(s) 748, 1024
BccI CCATC 2 cut(s) 812, 1163
BciT130I CCWGG 1 cut(s) 624
BciVI GTATCC 1 cut(s) 565
BclI TGATCA 1 cut(s) 429
BcoDI GTCTC 2 cut(s) 387, 1189
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 2 cut(s) 780, 1011
BfuI GTATCC 1 cut(s) 565
BisI GCNGC 2 cut(s) 737, 1038
BlnI CCTAGG 1 cut(s) 314
BlsI GCNGC 2 cut(s) 738, 1039
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 3 cut(s) 564, 600, 975
BmrFI CCNGG 1 cut(s) 624
BmrI ACTGGG 1 cut(s) 989
BmsI GCATC 3 cut(s) 874, 1049, 1061
BmuI ACTGGG 1 cut(s) 989
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 250
BpuEI CTTGAG 2 cut(s) 465, 761
BsaJI CCNNGG 2 cut(s) 314, 1111
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 629, 1117
Bse118I RCCGGY 2 cut(s) 35, 406
Bse1I ACTGG 1 cut(s) 984
Bse21I CCTNAGG 1 cut(s) 147
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 2 cut(s) 314, 1111
BseLI CCNNNNNNNGG 3 cut(s) 39, 629, 1117
BseMII CTCAG 5 cut(s) 161, 199, 398, 402, 1091
BseNI ACTGG 1 cut(s) 984
BseRI GAGGAG 2 cut(s) 129, 1001
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 2 cut(s) 748, 1024
BsgI GTGCAG 1 cut(s) 755
Bsh1236I CGCG 1 cut(s) 732
BshFI GGCC 1 cut(s) 812
BshNI GGYRCC 2 cut(s) 598, 973
BsiHKAI GWGCWC 1 cut(s) 756
BsiSI CCGG 3 cut(s) 36, 65, 407
BsiWI CGTACG 1 cut(s) 121
BslFI GGGAC 3 cut(s) 576, 1017, 1186
BslI CCNNNNNNNGG 3 cut(s) 39, 629, 1117
BsmAI GTCTC 2 cut(s) 387, 1189
BsmFI GGGAC 3 cut(s) 576, 1017, 1186
BsnI GGCC 1 cut(s) 812
Bsp1286I GDGCHC 2 cut(s) 601, 756
Bsp1407I TGTACA 1 cut(s) 1123
Bsp143I GATC 5 cut(s) 172, 188, 429, 502, 831
BspACI CCGC 5 cut(s) 39, 143, 763, 1035, 1192
BspANI GGCC 1 cut(s) 812
BspCNI CTCAG 5 cut(s) 160, 198, 397, 403, 1090
BspFNI CGCG 1 cut(s) 732
BspLI GGNNCC 3 cut(s) 564, 600, 975
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 2 cut(s) 183, 510
BspT107I GGYRCC 2 cut(s) 598, 973
BsrFI RCCGGY 2 cut(s) 35, 406
BsrGI TGTACA 1 cut(s) 1123
BsrI ACTGG 1 cut(s) 984
BssAI RCCGGY 2 cut(s) 35, 406
BssECI CCNNGG 2 cut(s) 314, 1111
BssMI GATC 5 cut(s) 172, 188, 429, 502, 831
BssT1I CCWWGG 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 4 cut(s) 13, 529, 781, 1138
Bst6I CTCTTC 1 cut(s) 210
BstAPI GCANNNNNTGC 1 cut(s) 884
BstAUI TGTACA 1 cut(s) 1123
BstC8I GCNNGC 1 cut(s) 734
BstDEI CTNAG 5 cut(s) 147, 185, 384, 411, 1077
BstENI CCTNNNNNAGG 1 cut(s) 627
BstFNI CGCG 1 cut(s) 732
BstHHI GCGC 1 cut(s) 732
BstKTI GATC 5 cut(s) 175, 191, 432, 505, 834
BstMAI GTCTC 2 cut(s) 387, 1189
BstMBI GATC 5 cut(s) 172, 188, 429, 502, 831
BstMWI GCNNNNNNNGC 5 cut(s) 142, 736, 760, 884, 1043
BstNI CCWGG 1 cut(s) 624
BstSCI CCNGG 1 cut(s) 622
BstSFI CTRYAG 2 cut(s) 780, 1011
BstSLI GKGCMC 1 cut(s) 601
BstUI CGCG 1 cut(s) 732
BstV1I GCAGC 2 cut(s) 748, 1024
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 2 cut(s) 188, 502
BstYI RGATCY 2 cut(s) 188, 502
Bsu36I CCTNAGG 1 cut(s) 147
BsuI GTATCC 1 cut(s) 565
BsuRI GGCC 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 1182
BtsIMutI CAGTG 2 cut(s) 439, 977
Cac8I GCNNGC 1 cut(s) 734
CaiI CAGNNNCTG 1 cut(s) 1049
CfoI GCGC 1 cut(s) 732
Cfr10I RCCGGY 2 cut(s) 35, 406
Cfr13I GGNCC 1 cut(s) 563
CseI GACGC 1 cut(s) 341
Csp6I GTAC 3 cut(s) 122, 615, 1124
CviAII CATG 3 cut(s) 395, 540, 865
CviQI GTAC 3 cut(s) 122, 615, 1124
DdeI CTNAG 5 cut(s) 147, 185, 384, 411, 1077
DpnI GATC 5 cut(s) 174, 190, 431, 504, 833
DpnII GATC 5 cut(s) 172, 188, 429, 502, 831
DraI TTTAAA 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 563
Eco57I CTGAAG 1 cut(s) 602
Eco81I CCTNAGG 1 cut(s) 147
EcoNI CCTNNNNNAGG 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 3 cut(s) 398, 543, 868
FaiI YATR 7 cut(s) 353, 396, 541, 569, 695, 866, 918
FaqI GGGAC 3 cut(s) 576, 1017, 1186
FatI CATG 3 cut(s) 394, 539, 864
FauI CCCGC 2 cut(s) 32, 1042
FbaI TGATCA 1 cut(s) 429
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 2 cut(s) 737, 1038
Fsp4HI GCNGC 2 cut(s) 737, 1038
FspBI CTAG 1 cut(s) 315
GlaI GCGC 1 cut(s) 731
GluI GCNGC 2 cut(s) 737, 1038
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 1 cut(s) 812
HapII CCGG 3 cut(s) 36, 65, 407
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 732
Hin1II CATG 3 cut(s) 398, 543, 868
Hin6I GCGC 1 cut(s) 730
HinP1I GCGC 1 cut(s) 730
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 6 cut(s) 23, 116, 251, 656, 1174, 1197
HpaII CCGG 3 cut(s) 36, 65, 407
HphI GGTGA 7 cut(s) 25, 388, 396, 638, 907, 917, 1192
Hpy166II GTNNAC 1 cut(s) 841
Hpy188I TCNGA 9 cut(s) 28, 172, 177, 188, 256, 309, 667, 967, 1080
Hpy188III TCNNGA 6 cut(s) 149, 267, 347, 641, 1104, 1154
Hpy8I GTNNAC 1 cut(s) 841
Hpy99I CGWCG 3 cut(s) 59, 62, 134
HpyAV CCTTC 3 cut(s) 493, 623, 1173
HpyCH4III ACNGT 4 cut(s) 13, 529, 781, 1138
HpyCH4IV ACGT 2 cut(s) 613, 891
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 736, 760, 884, 1043
HpyF3I CTNAG 5 cut(s) 147, 185, 384, 411, 1077
HpySE526I ACGT 2 cut(s) 613, 891
Hsp92II CATG 3 cut(s) 398, 543, 868
HspAI GCGC 1 cut(s) 730
Ksp22I TGATCA 1 cut(s) 429
Kzo9I GATC 5 cut(s) 172, 188, 429, 502, 831
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 2 cut(s) 748, 1024
LweI GCATC 3 cut(s) 874, 1049, 1061
MaeI CTAG 1 cut(s) 315
MaeII ACGT 2 cut(s) 613, 891
MalI GATC 5 cut(s) 174, 190, 431, 504, 833
MboI GATC 5 cut(s) 172, 188, 429, 502, 831
MflI RGATCY 2 cut(s) 188, 502
MhlI GDGCHC 2 cut(s) 601, 756
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 6 cut(s) 180, 260, 270, 324, 745, 787
MluNI TGGCCA 1 cut(s) 812
MlyI GAGTC 3 cut(s) 110, 1168, 1191
Mox20I TGGCCA 1 cut(s) 812
MroXI GAANNNNTTC 1 cut(s) 633
MscI TGGCCA 1 cut(s) 812
MseI TTAA 6 cut(s) 276, 365, 420, 491, 708, 1210
MslI CAYNNNNRTG 1 cut(s) 365
Msp20I TGGCCA 1 cut(s) 812
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 3 cut(s) 36, 65, 407
MspR9I CCNGG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 624
MvnI CGCG 1 cut(s) 732
MwoI GCNNNNNNNGC 5 cut(s) 142, 736, 760, 884, 1043
NdeII GATC 5 cut(s) 172, 188, 429, 502, 831
NlaIII CATG 3 cut(s) 398, 543, 868
NlaIV GGNNCC 3 cut(s) 564, 600, 975
NmeAIII GCCGAG 1 cut(s) 894
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 3 cut(s) 23, 251, 656
Pfl23II CGTACG 1 cut(s) 121
PkrI GCNGC 2 cut(s) 738, 1039
PleI GAGTC 3 cut(s) 110, 1168, 1191
PpsI GAGTC 3 cut(s) 110, 1168, 1191
PpuMI RGGWCCY 1 cut(s) 563
PshBI ATTAAT 1 cut(s) 276
PsiI TTATAA 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspLI CGTACG 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 564, 600, 975
PspPI GGNCC 1 cut(s) 563
PspPPI RGGWCCY 1 cut(s) 563
PstI CTGCAG 1 cut(s) 1015
PstNI CAGNNNCTG 1 cut(s) 1049
PsuI RGATCY 2 cut(s) 188, 502
RsaI GTAC 3 cut(s) 123, 616, 1125
RsaNI GTAC 3 cut(s) 122, 615, 1124
RseI CAYNNNNRTG 1 cut(s) 365
SalI GTCGAC 1 cut(s) 839
SaqAI TTAA 6 cut(s) 276, 365, 420, 491, 708, 1210
SatI GCNGC 2 cut(s) 737, 1038
Sau3AI GATC 5 cut(s) 172, 188, 429, 502, 831
Sau96I GGNCC 1 cut(s) 563
SchI GAGTC 3 cut(s) 110, 1168, 1191
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 2 cut(s) 601, 756
SfaNI GCATC 3 cut(s) 874, 1049, 1061
SfcI CTRYAG 2 cut(s) 780, 1011
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 365
SmlI CTYRAG 2 cut(s) 480, 740
SmoI CTYRAG 2 cut(s) 480, 740
Sse9I AATT 6 cut(s) 180, 260, 270, 324, 745, 787
SsiI CCGC 5 cut(s) 39, 143, 763, 1035, 1192
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 4 cut(s) 13, 529, 781, 1138
TaiI ACGT 2 cut(s) 616, 894
TaqI TCGA 3 cut(s) 840, 1103, 1177
TasI AATT 6 cut(s) 180, 260, 270, 324, 745, 787
TatI WGTACW 1 cut(s) 1123
TfiI GAWTC 3 cut(s) 23, 251, 656
Tru1I TTAA 6 cut(s) 276, 365, 420, 491, 708, 1210
Tru9I TTAA 6 cut(s) 276, 365, 420, 491, 708, 1210
TscAI CASTG 2 cut(s) 439, 984
TseI GCWGC 2 cut(s) 736, 1037
TspDTI ATGAA 3 cut(s) 257, 486, 1011
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 2 cut(s) 439, 984
VpaK11BI GGWCC 1 cut(s) 563
VspI ATTAAT 1 cut(s) 276
XagI CCTNNNNNAGG 1 cut(s) 627
XapI RAATTY 4 cut(s) 180, 260, 745, 787
XmaJI CCTAGG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.