pycom12g05160

Non-structural maintenance of chromosomes element 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000940
Physical Location & Seq
Reverse (-)
5035453 .. 5036762
1310 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05160.5

Sequence Viewer

Length: 858 bp
ATGAGCTCCGTCAAGGCACATAACAAGGAAGGAATCACAACTACAGGTTTCGTCAACTGTATTCTCAAACAGTTTGAGAAACGAAGTCAAAGTGGAAGCAGCTCAGTCTCTTGGAAGGATATTGGAAGGGCAGTCTCACATGTTTACCAGAAACCTCCCCAATGCTGCACTATGATTGGACCTATGAGTCTTGAAGTAAAGCAACGGAAGAGTTATGTTCGTGCAAAACGCGTGAAGCCTACAGAAAATGATACCCCTGAAGAGCTTCATGATGCTGTTGAAGAAGAGCAACCGGAGACTGTTAAAAACGTGTCAGCTATGTTCAATATCTTGAGGAAGAAGAGAGGAGTTAGGTTTGAAAATCTAGTGTTGAATGGGAATTCTTTTGCGCAGACGGTGGAAAATATATTTGCCCTCTCGTTTCTAGTTAAAGATGGGCGGGCGGAACTAAAATTGAATGAGGAAGGCCATCATCTAGTTTTGCCAAGAAATGCTCCTTCTGCTGAACAAATTGCTTCTGGGGAGATTACTTACAGACACTTTGTCTTCCGATTTGATTTCCAGGACTGGAAGTTCATGAAAGAGATTGTTGGGGACGGCGAGCAGCTGATGCCACGCAGGACCTATGAAGTAAACAAATCAAGCGATCATCAAGGCGATTTGCAATGCAAAGAATCTCAAACAACGCCAATTAGGAAGCTCTCCAGAAACAGAGGCTTGGTTATGCAGGAGCAGCCAGTTGGGGAAGGCATGGCTGAGCCTGATACTAATCAATCGATTGTGGAAGACTCGCCTGTACGCGATGACGCTCAAGAAAGCACCAATGCCATCCGAAAAGGGAAGCGTAAAATGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

286

Amino Acids

32.4

Weight (kDa)

8.78

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 186
Acc16I TGCGCA 1 cut(s) 390
AccII CGCG 2 cut(s) 231, 801
AciI CCGC 2 cut(s) 439, 443
AcsI RAATTY 1 cut(s) 379
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 1 cut(s) 798
AflIII ACRYGT 3 cut(s) 139, 229, 309
AgsI TTSAA 6 cut(s) 194, 281, 325, 359, 373, 457
AhdI GACNNNNNGTC 1 cut(s) 542
AjnI CCWGG 1 cut(s) 561
AloI GAACNNNNNNTCC 2 cut(s) 557, 589
AluBI AGCT 6 cut(s) 6, 102, 265, 317, 607, 700
AluI AGCT 6 cut(s) 6, 102, 265, 317, 607, 700
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 3 cut(s) 112, 139, 290
AoxI GGCC 1 cut(s) 466
ApeKI GCWGC 4 cut(s) 99, 165, 604, 733
ApoI RAATTY 1 cut(s) 379
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 179, 621
AvaII GGWCC 2 cut(s) 179, 621
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 538, 792
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 111, 152, 616, 745
BccI CCATC 3 cut(s) 428, 477, 836
BceAI ACGGC 1 cut(s) 613
BciT130I CCWGG 1 cut(s) 563
BcoDI GTCTC 3 cut(s) 112, 139, 290
BfaI CTAG 3 cut(s) 365, 425, 476
BfmI CTRYAG 2 cut(s) 42, 240
BisI GCNGC 4 cut(s) 100, 166, 605, 734
BlpI GCTNAGC 1 cut(s) 756
BlsI GCNGC 4 cut(s) 101, 167, 606, 735
Bme1390I CCNGG 1 cut(s) 563
Bme18I GGWCC 2 cut(s) 179, 621
BmeRI GACNNNNNGTC 1 cut(s) 542
BmgT120I GGNCC 2 cut(s) 179, 621
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 262, 600
BpiI GAAGAC 2 cut(s) 538, 792
BpmI CTGGAG 1 cut(s) 688
Bpu1102I GCTNAGC 1 cut(s) 756
BpuEI CTTGAG 2 cut(s) 352, 795
Bsa29I ATCGAT 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 768
BsaWI WCCGGW 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 572, 737
Bse3DI GCAATG 1 cut(s) 671
Bse8I GATNNNNATC 1 cut(s) 768
BseBI CCWGG 1 cut(s) 563
BseCI ATCGAT 1 cut(s) 776
BseGI GGATG 1 cut(s) 828
BseJI GATNNNNATC 1 cut(s) 768
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 2 cut(s) 117, 747
BseNI ACTGG 2 cut(s) 572, 737
BseRI GAGGAG 1 cut(s) 360
BseXI GCAGC 4 cut(s) 111, 152, 616, 745
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 2 cut(s) 231, 801
BshFI GGCC 1 cut(s) 468
BshVI ATCGAT 1 cut(s) 776
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 608
BsmAI GTCTC 3 cut(s) 112, 139, 290
BsmFI GGGAC 1 cut(s) 608
BsnI GGCC 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 646
Bsp1720I GCTNAGC 1 cut(s) 756
BspACI CCGC 2 cut(s) 439, 443
BspANI GGCC 1 cut(s) 468
BspCNI CTCAG 2 cut(s) 116, 748
BspDI ATCGAT 1 cut(s) 776
BspFNI CGCG 2 cut(s) 231, 801
BspHI TCATGA 2 cut(s) 268, 576
BspQI GCTCTTC 2 cut(s) 255, 279
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 2 cut(s) 572, 737
BssMI GATC 1 cut(s) 646
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 4 cut(s) 59, 72, 301, 397
Bst6I CTCTTC 4 cut(s) 203, 255, 279, 335
BstAPI GCANNNNNTGC 1 cut(s) 610
BstC8I GCNNGC 2 cut(s) 441, 602
BstDEI CTNAG 2 cut(s) 103, 756
BstF5I GGATG 1 cut(s) 828
BstFNI CGCG 2 cut(s) 231, 801
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 1 cut(s) 649
BstMAI GTCTC 3 cut(s) 112, 139, 290
BstMBI GATC 1 cut(s) 646
BstMWI GCNNNNNNNGC 3 cut(s) 500, 610, 733
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 561
BstSFI CTRYAG 2 cut(s) 42, 240
BstUI CGCG 2 cut(s) 231, 801
BstV1I GCAGC 4 cut(s) 111, 152, 616, 745
BstV2I GAAGAC 2 cut(s) 538, 792
Bsu15I ATCGAT 1 cut(s) 776
BsuRI GGCC 1 cut(s) 468
BsuTUI ATCGAT 1 cut(s) 776
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 1 cut(s) 828
Cac8I GCNNGC 2 cut(s) 441, 602
CciI TCATGA 2 cut(s) 268, 576
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 179, 621
ClaI ATCGAT 1 cut(s) 776
CseI GACGC 1 cut(s) 815
Csp6I GTAC 1 cut(s) 797
CviAII CATG 4 cut(s) 140, 269, 577, 751
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 2 cut(s) 103, 756
DpnI GATC 1 cut(s) 648
DpnII GATC 1 cut(s) 646
DrdI GACNNNNNNGTC 1 cut(s) 186
DriI GACNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 186
Eam1104I CTCTTC 4 cut(s) 203, 255, 279, 335
Eam1105I GACNNNNNGTC 1 cut(s) 542
EarI CTCTTC 4 cut(s) 203, 255, 279, 335
EciI GGCGGA 1 cut(s) 458
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 179, 621
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 621
EcoRI GAATTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 4 cut(s) 143, 272, 580, 754
FaqI GGGAC 1 cut(s) 608
FatI CATG 4 cut(s) 139, 268, 576, 750
FauI CCCGC 1 cut(s) 432
Fnu4HI GCNGC 4 cut(s) 100, 166, 605, 734
FokI GGATG 1 cut(s) 815
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 4 cut(s) 100, 166, 605, 734
FspBI CTAG 3 cut(s) 365, 425, 476
FspI TGCGCA 1 cut(s) 390
GlaI GCGC 1 cut(s) 390
GluI GCNGC 4 cut(s) 100, 166, 605, 734
GsuI CTGGAG 1 cut(s) 688
HaeIII GGCC 1 cut(s) 468
HapII CCGG 1 cut(s) 293
HgaI GACGC 1 cut(s) 815
HhaI GCGC 1 cut(s) 391
Hin1II CATG 4 cut(s) 143, 272, 580, 754
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 4 cut(s) 33, 187, 674, 788
HpaII CCGG 1 cut(s) 293
Hpy166II GTNNAC 3 cut(s) 55, 145, 634
Hpy188I TCNGA 2 cut(s) 551, 833
Hpy188III TCNNGA 6 cut(s) 191, 269, 331, 577, 705, 812
Hpy8I GTNNAC 3 cut(s) 55, 145, 634
HpyAV CCTTC 6 cut(s) 23, 109, 120, 458, 507, 740
HpyCH4III ACNGT 4 cut(s) 59, 72, 301, 397
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 5 cut(s) 168, 224, 664, 669, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 610, 733
HpyF3I CTNAG 2 cut(s) 103, 756
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 4 cut(s) 143, 272, 580, 754
HspAI GCGC 1 cut(s) 389
Kzo9I GATC 1 cut(s) 646
LguI GCTCTTC 2 cut(s) 255, 279
LmnI GCTCC 3 cut(s) 11, 499, 730
Lsp1109I GCAGC 4 cut(s) 111, 152, 616, 745
LweI GCATC 2 cut(s) 262, 600
MaeI CTAG 3 cut(s) 365, 425, 476
MaeII ACGT 1 cut(s) 309
MalI GATC 1 cut(s) 648
MboI GATC 1 cut(s) 646
MboII GAAGA 8 cut(s) 220, 272, 293, 296, 349, 352, 538, 797
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 4 cut(s) 379, 452, 510, 690
MluI ACGCGT 1 cut(s) 229
MlyI GAGTC 2 cut(s) 196, 782
MnlI CCTC 6 cut(s) 165, 327, 338, 425, 454, 707
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 2 cut(s) 303, 429
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 563
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 2 cut(s) 231, 801
MwoI GCNNNNNNNGC 3 cut(s) 500, 610, 733
NdeII GATC 1 cut(s) 646
NlaIII CATG 4 cut(s) 143, 272, 580, 754
NsbI TGCGCA 1 cut(s) 390
NspI RCATGY 1 cut(s) 143
PagI TCATGA 2 cut(s) 268, 576
PciI ACATGT 1 cut(s) 139
PciSI GCTCTTC 2 cut(s) 255, 279
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 33, 674
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 4 cut(s) 101, 167, 606, 735
PleI GAGTC 2 cut(s) 195, 782
PpsI GAGTC 2 cut(s) 195, 782
PpuMI RGGWCCY 1 cut(s) 621
PscI ACATGT 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspPI GGNCC 2 cut(s) 179, 621
PspPPI RGGWCCY 1 cut(s) 621
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 2 cut(s) 255, 279
SaqAI TTAA 2 cut(s) 303, 429
SatI GCNGC 4 cut(s) 100, 166, 605, 734
Sau3AI GATC 1 cut(s) 646
Sau96I GGNCC 2 cut(s) 179, 621
SchI GAGTC 2 cut(s) 196, 782
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 1 cut(s) 8
SfaNI GCATC 2 cut(s) 262, 600
SfcI CTRYAG 2 cut(s) 42, 240
SinI GGWCC 2 cut(s) 179, 621
SmlI CTYRAG 2 cut(s) 331, 810
SmoI CTYRAG 2 cut(s) 331, 810
Sse9I AATT 4 cut(s) 379, 452, 510, 690
SsiI CCGC 2 cut(s) 439, 443
SspMI CTAG 3 cut(s) 365, 425, 476
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 561
TaaI ACNGT 4 cut(s) 59, 72, 301, 397
TaiI ACGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 776
TasI AATT 4 cut(s) 379, 452, 510, 690
TfiI GAWTC 2 cut(s) 33, 674
Tru1I TTAA 2 cut(s) 303, 429
Tru9I TTAA 2 cut(s) 303, 429
TseI GCWGC 4 cut(s) 99, 165, 604, 733
TspDTI ATGAA 4 cut(s) 257, 565, 593, 642
TspGWI ACGGA 1 cut(s) 220
VpaK11BI GGWCC 2 cut(s) 179, 621
XapI RAATTY 1 cut(s) 379
XceI RCATGY 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 3 cut(s) 365, 425, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.