RLG00000023530

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000940
Physical Location & Seq
Forward (+)
24974207 .. 24976109
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023530

Sequence Viewer

Length: 1125 bp
ATGGTGAAACGCGAATTGGGTTGTGCTACTAGTAGCGGAGGAGATGAAACGGACGAACCTGATCACGCGTCGCCGGCTGTGGCTGACCCTAGAGCACTCCGATCACGCTACCACACAGTCAAGACTATCCTCTACGATGAAAGAGAGGATCTCACAAGAGCAGACTCGGAGAAGTTCAATTCGATCATTAAGGAAGTGGAGAGCTTGCACCGGCTGGTAACAAAGCCAAGAGAGCAAATTGCGGATGCGGAGGCGCTTTTGGACATTGCAAGCACCCTGATGACCTCTATAAGGGCACATAACAAGGAAGGAATTACAGCTTCGGGTTTCGTCAACTGTATTCTTAGTGACTTCAGGAAACCGAGTGGTAATGGCAGCAGCTCAATATCTTGGAGGGACATTGGGAATGCTGTATCACATGTTTTCCAGAGAAGCCCTCGATGCTGCACTATGATTGGGCCTATGAATGCTGATGTGAAGCGACGAAAGGTTTATCAACGTCAAAAACGTGGGAATCCTACAGACAATGATACCCCTAAAGAGCTTGGTGATGCTGTTGTAGAAGAGGAAACAGAGACTGTTAAAAATGTGGCAACTATGTTTAACATTTTGAAGAAAAAGAGAAGGGTCAGGCTTGAATATCTAGTTTTGAATGGAGATTCTTTTTCACAGACAGTGGAAAATGTGTTTGCCCTATCGTTTCTTGTTAAAGATGGACGAGTCGAAATAACAGTGAATGAGGAAGGCCATCATCTAGTGTCGCCAAGGAATGCTCCTGCTGCCGAAGCAATTGCCTCAGGCGCTGTTACTTACTGCCACTTAATCTTCAGATTTGATTTCAAGGACTGGAAGTTAATGAAAGAAGTTGTTGGGAATGGGGAGGAGCTGATGCCACATAGGTTTCCAGCTGACGATGTATCAAACAATTCGCAACTGGAAGAACCTCAAGCAACAACAGGTACAACACCAATTAGGAAGTTCTCAAGGAACAGAGGCTTGGTTATACGGGAGCAGCCCGTTGGGACTGATAGTAGTAGCAGATCAACTGCTGAAGACAGCGCTGAATGTGATGATTATGCTGCAAGCGCTTCTGCCACTCGTAAAGGGAAGCGTAAACTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

375

Amino Acids

41.56

Weight (kDa)

6.78

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 210 - 297 9.1e-22 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 12, 68
AciI CCGC 3 cut(s) 36, 242, 248
AclWI GGATC 1 cut(s) 156
AcuI CTGAAG 3 cut(s) 337, 811, 1071
AfaI GTAC 1 cut(s) 961
AfeI AGCGCT 2 cut(s) 1060, 1087
AfiI CCNNNNNNNGG 1 cut(s) 291
AflIII ACRYGT 2 cut(s) 66, 418
AgsI TTSAA 5 cut(s) 178, 613, 638, 652, 841
AhlI ACTAGT 1 cut(s) 29
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 980, 1012
AluBI AGCT 6 cut(s) 204, 320, 381, 544, 886, 908
AluI AGCT 6 cut(s) 204, 320, 381, 544, 886, 908
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 1 cut(s) 156
AlwNI CAGNNNCTG 2 cut(s) 578, 803
Aor51HI AGCGCT 2 cut(s) 1060, 1087
AoxI GGCC 2 cut(s) 458, 745
ApeKI GCWGC 6 cut(s) 375, 378, 444, 779, 1012, 1079
AspLEI GCGC 4 cut(s) 256, 803, 1061, 1088
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 16, 560
AxyI CCTNAGG 1 cut(s) 796
BaeGI GKGCMC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 1059
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 6 cut(s) 387, 390, 431, 766, 1024, 1066
BccI CCATC 2 cut(s) 707, 756
BcgI CGANNNNNNTGC 2 cut(s) 773, 807
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 569
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 4 cut(s) 30, 90, 644, 755
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 4 cut(s) 257, 804, 1062, 1089
BisI GCNGC 6 cut(s) 376, 379, 445, 780, 1013, 1080
BlsI GCNGC 6 cut(s) 377, 380, 446, 781, 1014, 1081
BmgT120I GGNCC 1 cut(s) 458
BmsI GCATC 4 cut(s) 235, 431, 541, 879
BpiI GAAGAC 1 cut(s) 1059
BplI GAGNNNNNCTC 4 cut(s) 135, 167, 421, 453
BpuEI CTTGAG 2 cut(s) 930, 967
BsaJI CCNNGG 1 cut(s) 764
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse118I RCCGGY 2 cut(s) 73, 210
Bse1I ACTGG 2 cut(s) 851, 939
Bse21I CCTNAGG 1 cut(s) 796
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 1 cut(s) 810
BseNI ACTGG 2 cut(s) 851, 939
BseRI GAGGAG 2 cut(s) 54, 896
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 6 cut(s) 387, 390, 431, 766, 1024, 1066
BsgI GTGCAG 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 12, 68
BshFI GGCC 2 cut(s) 460, 747
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 2 cut(s) 74, 211
BslFI GGGAC 2 cut(s) 410, 1036
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 2 cut(s) 410, 1036
BsmI GAATGC 3 cut(s) 412, 472, 775
BsnI GGCC 2 cut(s) 460, 747
Bsp1286I GDGCHC 2 cut(s) 97, 298
Bsp143I GATC 5 cut(s) 61, 101, 148, 183, 1040
BspACI CCGC 3 cut(s) 36, 242, 248
BspANI GGCC 2 cut(s) 460, 747
BspCNI CTCAG 1 cut(s) 809
BspFNI CGCG 2 cut(s) 12, 68
BspPI GGATC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 2 cut(s) 73, 210
BsrI ACTGG 2 cut(s) 851, 939
BssAI RCCGGY 2 cut(s) 73, 210
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 5 cut(s) 61, 101, 148, 183, 1040
BssT1I CCWWGG 1 cut(s) 764
Bst4CI ACNGT 5 cut(s) 118, 338, 580, 676, 733
Bst6I CTCTTC 1 cut(s) 558
BstC8I GCNNGC 4 cut(s) 75, 206, 271, 1084
BstDEI CTNAG 2 cut(s) 344, 796
BstENI CCTNNNNNAGG 1 cut(s) 289
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 2 cut(s) 12, 68
BstH2I RGCGCY 4 cut(s) 257, 804, 1062, 1089
BstHHI GCGC 4 cut(s) 256, 803, 1061, 1088
BstKTI GATC 5 cut(s) 64, 104, 151, 186, 1043
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 5 cut(s) 61, 101, 148, 183, 1040
BstMWI GCNNNNNNNGC 7 cut(s) 74, 232, 441, 779, 785, 800, 1085
BstNSI RCATGY 1 cut(s) 422
BstSFI CTRYAG 1 cut(s) 519
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 2 cut(s) 12, 68
BstV1I GCAGC 6 cut(s) 387, 390, 431, 766, 1024, 1066
BstV2I GAAGAC 1 cut(s) 1059
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 796
BsuRI GGCC 2 cut(s) 460, 747
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 2 cut(s) 681, 738
Cac8I GCNNGC 4 cut(s) 75, 206, 271, 1084
CaiI CAGNNNCTG 2 cut(s) 578, 803
CfoI GCGC 4 cut(s) 256, 803, 1061, 1088
Cfr10I RCCGGY 2 cut(s) 73, 210
Cfr13I GGNCC 1 cut(s) 458
CseI GACGC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 960
CviAII CATG 1 cut(s) 419
CviQI GTAC 1 cut(s) 960
DdeI CTNAG 2 cut(s) 344, 796
DpnI GATC 5 cut(s) 63, 103, 150, 185, 1042
DpnII GATC 5 cut(s) 61, 101, 148, 183, 1040
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 764
Eco47III AGCGCT 2 cut(s) 1060, 1087
Eco57I CTGAAG 3 cut(s) 337, 811, 1071
Eco81I CCTNAGG 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 764
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 1 cut(s) 422
FaqI GGGAC 2 cut(s) 410, 1036
FatI CATG 1 cut(s) 418
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 6 cut(s) 376, 379, 445, 780, 1013, 1080
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 6 cut(s) 376, 379, 445, 780, 1013, 1080
FspBI CTAG 4 cut(s) 30, 90, 644, 755
GlaI GCGC 4 cut(s) 255, 802, 1060, 1087
GluI GCNGC 6 cut(s) 376, 379, 445, 780, 1013, 1080
HaeII RGCGCY 4 cut(s) 257, 804, 1062, 1089
HaeIII GGCC 2 cut(s) 460, 747
HapII CCGG 2 cut(s) 74, 211
HgaI GACGC 1 cut(s) 57
HhaI GCGC 4 cut(s) 256, 803, 1061, 1088
Hin1II CATG 1 cut(s) 422
Hin6I GCGC 4 cut(s) 254, 801, 1059, 1086
HinP1I GCGC 4 cut(s) 254, 801, 1059, 1086
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 4 cut(s) 164, 514, 659, 720
HpaII CCGG 2 cut(s) 74, 211
HphI GGTGA 2 cut(s) 16, 560
Hpy166II GTNNAC 2 cut(s) 334, 1115
Hpy188I TCNGA 3 cut(s) 101, 169, 830
Hpy188III TCNNGA 3 cut(s) 121, 355, 427
Hpy8I GTNNAC 2 cut(s) 334, 1115
Hpy99I CGWCG 2 cut(s) 73, 486
HpyAV CCTTC 3 cut(s) 302, 618, 737
HpyCH4III ACNGT 5 cut(s) 118, 338, 580, 676, 733
HpyCH4IV ACGT 2 cut(s) 499, 508
HpyCH4V TGCA 4 cut(s) 208, 269, 447, 1082
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 232, 441, 779, 785, 800, 1085
HpyF3I CTNAG 2 cut(s) 344, 796
HpySE526I ACGT 2 cut(s) 499, 508
Hsp92II CATG 1 cut(s) 422
HspAI GCGC 4 cut(s) 254, 801, 1059, 1086
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 5 cut(s) 61, 101, 148, 183, 1040
LmnI GCTCC 3 cut(s) 778, 883, 1009
Lsp1109I GCAGC 6 cut(s) 387, 390, 431, 766, 1024, 1066
LweI GCATC 4 cut(s) 235, 431, 541, 879
MaeI CTAG 4 cut(s) 30, 90, 644, 755
MaeII ACGT 2 cut(s) 499, 508
MaeIII GTNAC 3 cut(s) 217, 347, 805
MalI GATC 5 cut(s) 63, 103, 150, 185, 1042
MboI GATC 5 cut(s) 61, 101, 148, 183, 1040
MboII GAAGA 5 cut(s) 575, 625, 817, 950, 1064
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 2 cut(s) 97, 298
MluCI AATT 7 cut(s) 14, 178, 237, 312, 789, 925, 969
MluI ACGCGT 1 cut(s) 66
MlyI GAGTC 2 cut(s) 158, 729
MroNI GCCGGC 1 cut(s) 73
MseI TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 908
MspI CCGG 2 cut(s) 74, 211
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 3 cut(s) 412, 472, 775
MvnI CGCG 2 cut(s) 12, 68
MwoI GCNNNNNNNGC 7 cut(s) 74, 232, 441, 779, 785, 800, 1085
NaeI GCCGGC 1 cut(s) 75
NdeII GATC 5 cut(s) 61, 101, 148, 183, 1040
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 1 cut(s) 422
NmuCI GTSAC 1 cut(s) 347
NspI RCATGY 1 cut(s) 422
PciI ACATGT 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PctI GAATGC 3 cut(s) 412, 472, 775
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 514, 659
PkrI GCNGC 6 cut(s) 377, 380, 446, 781, 1014, 1081
PleI GAGTC 2 cut(s) 158, 728
PpsI GAGTC 2 cut(s) 158, 728
PscI ACATGT 1 cut(s) 418
PspPI GGNCC 1 cut(s) 458
PstNI CAGNNNCTG 2 cut(s) 578, 803
PsuI RGATCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 1 cut(s) 961
RsaNI GTAC 1 cut(s) 960
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
SatI GCNGC 6 cut(s) 376, 379, 445, 780, 1013, 1080
Sau3AI GATC 5 cut(s) 61, 101, 148, 183, 1040
Sau96I GGNCC 1 cut(s) 458
SchI GAGTC 2 cut(s) 158, 729
SduI GDGCHC 2 cut(s) 97, 298
SfaNI GCATC 4 cut(s) 235, 431, 541, 879
SfcI CTRYAG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 278
SmlI CTYRAG 2 cut(s) 945, 982
SmoI CTYRAG 2 cut(s) 945, 982
SpeI ACTAGT 1 cut(s) 29
Sse9I AATT 7 cut(s) 14, 178, 237, 312, 789, 925, 969
SsiI CCGC 3 cut(s) 36, 242, 248
SspMI CTAG 4 cut(s) 30, 90, 644, 755
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 5 cut(s) 118, 338, 580, 676, 733
TaiI ACGT 2 cut(s) 502, 511
TaqI TCGA 3 cut(s) 182, 439, 723
TasI AATT 7 cut(s) 14, 178, 237, 312, 789, 925, 969
TfiI GAWTC 2 cut(s) 514, 659
Tru1I TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
Tru9I TTAA 6 cut(s) 189, 582, 603, 708, 821, 854
TscAI CASTG 2 cut(s) 681, 738
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 6 cut(s) 375, 378, 444, 779, 1012, 1079
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 4 cut(s) 60, 153, 479, 872
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 2 cut(s) 681, 738
XagI CCTNNNNNAGG 1 cut(s) 289
XceI RCATGY 1 cut(s) 422
XspI CTAG 4 cut(s) 30, 90, 644, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.