Rroxscaffold_1G00002920

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000940
Physical Location & Seq
Reverse (-)
3435033 .. 3440610
5578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002920.1

Sequence Viewer

Length: 1029 bp
ATGGCTGAGAAGGAGTGTACTGTCAATTTTGAGCTACTCAACTTCGGCCAAGACCCGGCGCCACGGCGAGAGCTCCGCTCCGATTACCTCGCCTTCCGGAGCTTCACTAGTGAGAAAAGAGACGATTTAATGAGTCCGGATTCGATGAAGTTTGATGTGATCATTGATATAATTGACAAACTGCATGAGCAAGTTCAGCTTCCGAGAGAACAAGTAGCGGATGCACAAGCACTTCTGGACATAAGTAACACAGTGGTGGCCTCTGTTATGTCACATTCTAATGGGGAAACTACCCCAAGCGATTTCATTACTGATTTGATTACATATTTCGGGCAAGTCAATAGGCCATCAGTGAACAAAGATAGTGCCCATGTCTCACTTAAATGGAAAGATATTGGACTTTCTGTTTCACCAATACTCAAGAGGGCTGTTGGATGTTGCACAATGCTTGGACCCATGAATATTCAAATTAAGCAAAGGAAGGCTGCTGTCCGGCGTACTAAGCCTACCACAACTCATCTGCCCAAGGAGATCAATAATGAAGGAGGCGAAAGAACTGATACAGATTTGAACATGGCAACAATGTTTGAGATTTTAAAGAACAAGAAAAGGGTGGAACTCAGTTGTTTGATACTAAATAGAAGATCTTTTGCACAGACAGTGGAGAATCTATTTGCTCTGTCATTTCTGGCAAAAGACGGCAGAGTTAGAATTGCTGTTGAAGCAAATGGTTCTCATATTATAATGATGGAAATGGTGGCTGTTGGTGAGGAGCTGATGCCTCATAGAGACAGCACCAACTGCACACCTGCCTCTGAGGAGCAACCAGCAGCATTCAATTCCCAAACAGCGACAGCCTTACACACAACCCGGATTCAAAAACTTACCAGAAACCATGCCCTGGTTGTGCAGGAAAAATCTACTGCAGAAGAATTCTTTGAAAATGATGATGCTGTTGGAGCCACAGAACCTCGCAGCTGTAAGCGCAGGCTTTCGTTTAATACATGTTTAAGCGGAAGCTTAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

342

Amino Acids

38.22

Weight (kDa)

6.28

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 205 - 257 7e-13 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AarI CACCTGC 1 cut(s) 817
Acc36I ACCTGC 1 cut(s) 817
AccB1I GGYRCC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 901
AccBSI CCGCTC 1 cut(s) 78
AccIII TCCGGA 2 cut(s) 96, 136
AciI CCGC 3 cut(s) 76, 218, 1014
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 932
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 2 cut(s) 19, 499
AfiI CCNNNNNNNGG 2 cut(s) 55, 901
AflII CTTAAG 1 cut(s) 1021
AflIII ACRYGT 1 cut(s) 1004
AgsI TTSAA 6 cut(s) 467, 571, 722, 838, 878, 941
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 1 cut(s) 900
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 7 cut(s) 34, 73, 102, 199, 775, 978, 1020
AluI AGCT 7 cut(s) 34, 73, 102, 199, 775, 978, 1020
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 3 cut(s) 114, 379, 783
Aor13HI TCCGGA 2 cut(s) 96, 136
AoxI GGCC 3 cut(s) 46, 258, 344
ApeKI GCWGC 3 cut(s) 485, 830, 975
ApoI RAATTY 1 cut(s) 932
AspLEI GCGC 2 cut(s) 61, 987
AspS9I GGNCC 1 cut(s) 452
AsuC2I CCSGG 2 cut(s) 56, 871
AsuHPI GGTGA 2 cut(s) 402, 779
AvaII GGWCC 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 3 cut(s) 472, 842, 987
BccI CCATC 2 cut(s) 355, 742
BceAI ACGGC 2 cut(s) 80, 715
BciT130I CCWGG 1 cut(s) 902
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 2 cut(s) 56, 871
BcoDI GTCTC 3 cut(s) 114, 379, 783
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 1 cut(s) 108
BfmI CTRYAG 1 cut(s) 924
BfoI RGCGCY 1 cut(s) 62
BfrI CTTAAG 1 cut(s) 1021
BfuAI ACCTGC 1 cut(s) 817
BglII AGATCT 1 cut(s) 644
BisI GCNGC 3 cut(s) 486, 831, 976
BlsI GCNGC 3 cut(s) 487, 832, 977
Bme1390I CCNGG 3 cut(s) 56, 871, 902
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 3 cut(s) 60, 454, 961
BmrFI CCNGG 3 cut(s) 56, 871, 902
BmsI GCATC 3 cut(s) 211, 768, 940
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 2 cut(s) 56, 871
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 62, 525, 900
BsaWI WCCGGW 2 cut(s) 96, 136
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 901
BseAI TCCGGA 2 cut(s) 96, 136
BseBI CCWGG 1 cut(s) 902
BseDI CCNNGG 3 cut(s) 62, 525, 900
BseGI GGATG 2 cut(s) 226, 440
BseLI CCNNNNNNNGG 2 cut(s) 55, 901
BseMII CTCAG 2 cut(s) 634, 807
BseRI GAGGAG 2 cut(s) 785, 833
BseSI GKGCMC 1 cut(s) 370
BseXI GCAGC 3 cut(s) 472, 842, 987
BsgI GTGCAG 2 cut(s) 787, 929
BshFI GGCC 3 cut(s) 48, 260, 346
BshNI GGYRCC 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 5 cut(s) 56, 97, 137, 493, 871
BslI CCNNNNNNNGG 2 cut(s) 55, 901
BsmAI GTCTC 3 cut(s) 114, 379, 783
BsmBI CGTCTC 1 cut(s) 114
BsmI GAATGC 1 cut(s) 833
BsnI GGCC 3 cut(s) 48, 260, 346
Bsp1286I GDGCHC 2 cut(s) 75, 370
Bsp13I TCCGGA 2 cut(s) 96, 136
Bsp143I GATC 3 cut(s) 159, 531, 644
BspACI CCGC 3 cut(s) 76, 218, 1014
BspANI GGCC 3 cut(s) 48, 260, 346
BspCNI CTCAG 2 cut(s) 633, 808
BspEI TCCGGA 2 cut(s) 96, 136
BspLI GGNNCC 3 cut(s) 60, 454, 961
BspMAI CTGCAG 1 cut(s) 928
BspMI ACCTGC 1 cut(s) 817
BspT107I GGYRCC 1 cut(s) 58
BspTI CTTAAG 1 cut(s) 1021
BsrBI CCGCTC 1 cut(s) 78
BssECI CCNNGG 3 cut(s) 62, 525, 900
BssMI GATC 3 cut(s) 159, 531, 644
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 902
Bst4CI ACNGT 3 cut(s) 22, 253, 661
BstACI GRCGYC 1 cut(s) 59
BstAFI CTTAAG 1 cut(s) 1021
BstAPI GCANNNNNTGC 1 cut(s) 801
BstC8I GCNNGC 1 cut(s) 989
BstDEI CTNAG 4 cut(s) 6, 501, 620, 816
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 226, 440
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 2 cut(s) 61, 987
BstKTI GATC 3 cut(s) 162, 534, 647
BstMAI GTCTC 3 cut(s) 114, 379, 783
BstMBI GATC 3 cut(s) 159, 531, 644
BstMWI GCNNNNNNNGC 6 cut(s) 196, 502, 722, 801, 959, 984
BstNI CCWGG 1 cut(s) 902
BstNSI RCATGY 1 cut(s) 1008
BstSCI CCNGG 3 cut(s) 54, 869, 900
BstSFI CTRYAG 1 cut(s) 924
BstSLI GKGCMC 1 cut(s) 370
BstV1I GCAGC 3 cut(s) 472, 842, 987
BstX2I RGATCY 1 cut(s) 644
BstYI RGATCY 1 cut(s) 644
BsuRI GGCC 3 cut(s) 48, 260, 346
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 226, 440
BtsIMutI CAGTG 3 cut(s) 258, 357, 666
BveI ACCTGC 1 cut(s) 817
Cac8I GCNNGC 1 cut(s) 989
CfoI GCGC 2 cut(s) 61, 987
Cfr13I GGNCC 1 cut(s) 452
Csp6I GTAC 2 cut(s) 18, 498
CviAII CATG 6 cut(s) 185, 371, 457, 574, 896, 1005
CviQI GTAC 2 cut(s) 18, 498
DdeI CTNAG 4 cut(s) 6, 501, 620, 816
DinI GGCGCC 1 cut(s) 60
DpnI GATC 3 cut(s) 161, 533, 646
DpnII GATC 3 cut(s) 159, 531, 644
DraI TTTAAA 1 cut(s) 597
EaeI YGGCCR 1 cut(s) 46
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 452
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 932
EcoRII CCWGG 1 cut(s) 900
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 75
EgeI GGCGCC 1 cut(s) 60
EheI GGCGCC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 6 cut(s) 188, 374, 460, 577, 899, 1008
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 6 cut(s) 184, 370, 456, 573, 895, 1004
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 486, 831, 976
FokI GGATG 2 cut(s) 233, 447
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 3 cut(s) 486, 831, 976
FspBI CTAG 1 cut(s) 108
GlaI GCGC 2 cut(s) 60, 986
GluI GCNGC 3 cut(s) 486, 831, 976
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 3 cut(s) 48, 260, 346
HapII CCGG 5 cut(s) 56, 97, 137, 493, 871
HhaI GCGC 2 cut(s) 61, 987
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 6 cut(s) 188, 374, 460, 577, 899, 1008
Hin6I GCGC 2 cut(s) 59, 985
HinP1I GCGC 2 cut(s) 59, 985
HindIII AAGCTT 1 cut(s) 1018
HinfI GANTC 4 cut(s) 133, 140, 667, 874
HpaII CCGG 5 cut(s) 56, 97, 137, 493, 871
HphI GGTGA 2 cut(s) 402, 779
Hpy166II GTNNAC 2 cut(s) 18, 355
Hpy188I TCNGA 3 cut(s) 82, 204, 817
Hpy188III TCNNGA 4 cut(s) 97, 137, 236, 421
Hpy8I GTNNAC 2 cut(s) 18, 355
HpyAV CCTTC 4 cut(s) 4, 103, 475, 536
HpyCH4III ACNGT 3 cut(s) 22, 253, 661
HpyCH4V TGCA 7 cut(s) 184, 224, 441, 653, 804, 910, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 196, 502, 722, 801, 959, 984
HpyF3I CTNAG 4 cut(s) 6, 501, 620, 816
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 6 cut(s) 188, 374, 460, 577, 899, 1008
HspAI GCGC 2 cut(s) 59, 985
KasI GGCGCC 1 cut(s) 58
Kpn2I TCCGGA 2 cut(s) 96, 136
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 531, 644
LmnI GCTCC 6 cut(s) 78, 83, 99, 772, 820, 959
Lsp1109I GCAGC 3 cut(s) 472, 842, 987
LweI GCATC 3 cut(s) 211, 768, 940
MaeI CTAG 1 cut(s) 108
MaeIII GTNAC 2 cut(s) 245, 270
MalI GATC 3 cut(s) 161, 533, 646
MbiI CCGCTC 1 cut(s) 78
MboI GATC 3 cut(s) 159, 531, 644
MboII GAAGA 2 cut(s) 654, 941
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 2 cut(s) 75, 370
MluCI AATT 6 cut(s) 25, 171, 468, 711, 838, 932
Mly113I GGCGCC 1 cut(s) 59
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 2 cut(s) 412, 937
MnlI CCTC 9 cut(s) 98, 271, 417, 539, 763, 792, 811, 823, 981
MroI TCCGGA 2 cut(s) 96, 136
MseI TTAA 7 cut(s) 128, 381, 471, 596, 999, 1010, 1022
MslI CAYNNNNRTG 3 cut(s) 254, 279, 382
MspA1I CMGCKG 1 cut(s) 978
MspCI CTTAAG 1 cut(s) 1021
MspI CCGG 5 cut(s) 56, 97, 137, 493, 871
MspR9I CCNGG 3 cut(s) 56, 871, 902
Mva1269I GAATGC 1 cut(s) 833
MvaI CCWGG 1 cut(s) 902
MwoI GCNNNNNNNGC 6 cut(s) 196, 502, 722, 801, 959, 984
NarI GGCGCC 1 cut(s) 59
NciI CCSGG 2 cut(s) 56, 871
NdeII GATC 3 cut(s) 159, 531, 644
NlaIII CATG 6 cut(s) 188, 374, 460, 577, 899, 1008
NlaIV GGNNCC 3 cut(s) 60, 454, 961
NmuCI GTSAC 1 cut(s) 270
NspI RCATGY 1 cut(s) 1008
OliI CACNNNNGTG 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 817
PciI ACATGT 1 cut(s) 1004
PctI GAATGC 1 cut(s) 833
PfeI GAWTC 3 cut(s) 140, 667, 874
PflMI CCANNNNNTGG 1 cut(s) 901
PkrI GCNGC 3 cut(s) 487, 832, 977
PleI GAGTC 1 cut(s) 141
PluTI GGCGCC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 141
PscI ACATGT 1 cut(s) 1004
PsiI TTATAA 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 900
PspGI CCWGG 1 cut(s) 900
PspN4I GGNNCC 3 cut(s) 60, 454, 961
PspPI GGNCC 1 cut(s) 452
PstI CTGCAG 1 cut(s) 928
PsuI RGATCY 1 cut(s) 644
PvuII CAGCTG 1 cut(s) 978
RsaI GTAC 2 cut(s) 19, 499
RsaNI GTAC 2 cut(s) 18, 498
RseI CAYNNNNRTG 3 cut(s) 254, 279, 382
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 7 cut(s) 128, 381, 471, 596, 999, 1010, 1022
SatI GCNGC 3 cut(s) 486, 831, 976
Sau3AI GATC 3 cut(s) 159, 531, 644
Sau96I GGNCC 1 cut(s) 452
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 3 cut(s) 56, 871, 902
SduI GDGCHC 2 cut(s) 75, 370
SfaNI GCATC 3 cut(s) 211, 768, 940
SfcI CTRYAG 1 cut(s) 924
SfoI GGCGCC 1 cut(s) 60
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 3 cut(s) 254, 279, 382
SmlI CTYRAG 2 cut(s) 419, 1021
SmoI CTYRAG 2 cut(s) 419, 1021
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 6 cut(s) 25, 171, 468, 711, 838, 932
SsiI CCGC 3 cut(s) 76, 218, 1014
SspDI GGCGCC 1 cut(s) 58
SspI AATATT 1 cut(s) 463
SspMI CTAG 1 cut(s) 108
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 3 cut(s) 54, 869, 900
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 3 cut(s) 22, 253, 661
TaqI TCGA 1 cut(s) 143
TasI AATT 6 cut(s) 25, 171, 468, 711, 838, 932
TatI WGTACW 1 cut(s) 17
TfiI GAWTC 3 cut(s) 140, 667, 874
Tru1I TTAA 7 cut(s) 128, 381, 471, 596, 999, 1010, 1022
Tru9I TTAA 7 cut(s) 128, 381, 471, 596, 999, 1010, 1022
TscAI CASTG 3 cut(s) 258, 357, 666
TseFI GTSAC 1 cut(s) 270
TseI GCWGC 3 cut(s) 485, 830, 975
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 4 cut(s) 161, 295, 473, 555
TspRI CASTG 3 cut(s) 258, 357, 666
Van91I CCANNNNNTGG 1 cut(s) 901
Vha464I CTTAAG 1 cut(s) 1021
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 1 cut(s) 932
XceI RCATGY 1 cut(s) 1008
XspI CTAG 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.