RchiOBHm_Chr5g0080481

Non-structural maintenance of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000940
Physical Location & Seq
Forward (+)
86462820 .. 86464808
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35479

Sequence Viewer

Length: 585 bp
ATGTCACATTCTAATGGGGAAACTACCCCAGGCGATTTCATTACTGGTTTAATTACATATTTCCGGCAAGTCAATAGGCCATCAGTGAACAAAGATAGTGCCCATGTCTCAAATGGAGAGATATTGGACTTTCTGTTTCACCAATACTCAAGAGGACTGTTGGATGTTGCACAATGTGAGCTTGGACCCATGAATATTCAGATTAAGCAAAGGAAGGCTGCTGTCCGGCGTACAAAGCCTACCACAACTCATCTGCCCAAGGAGATCAATAATGAAGGAGGCGAACGAACTGATACAGATTTGAACATGGCAACAATGTCTGAGATTTTAAAGAACAATAAAAGGGTGGAACTCAGTTGTTTGATACTAAATAGAAGATCTTTTGCACAGACAGTGGAGAATCTATTTGCTCTGTCATTTCTGGCAAAAGACGGCAGAGTTAGAATTGCTGTCGAAGCAAATGGTTCTCATATTGTTTCTCCTACGAATGGTCCCGTTGCCAATGAAGTGGCTTATCACCATTTTGTGTTCCGATTTGATTTCAAGGACTGGAAGATAATGATGGAAATGGTGGCTGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nse4_C

Protein Analysis

194

Amino Acids

21.98

Weight (kDa)

8.59

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nse4_C PF08743 116 - 191 9.7e-20 Nse4 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 304, 544
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 1 cut(s) 181
AluI AGCT 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 112
AoxI GGCC 1 cut(s) 77
ApeKI GCWGC 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 185, 491
AsuHPI GGTGA 2 cut(s) 131, 509
AvaII GGWCC 2 cut(s) 185, 491
BaeGI GKGCMC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 205
BccI CCATC 2 cut(s) 88, 556
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 112
BglII AGATCT 1 cut(s) 377
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 185, 491
BmgT120I GGNCC 2 cut(s) 185, 491
BmiI GGNNCC 2 cut(s) 187, 493
BmrFI CCNGG 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 28, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 2 cut(s) 49, 554
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 28, 258
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 2 cut(s) 312, 367
BseNI ACTGG 2 cut(s) 49, 554
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 1 cut(s) 205
BshFI GGCC 1 cut(s) 79
BsiSI CCGG 2 cut(s) 64, 226
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 477
BsnI GGCC 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 2 cut(s) 264, 377
BspANI GGCC 1 cut(s) 79
BspCNI CTCAG 2 cut(s) 313, 366
BspLI GGNNCC 2 cut(s) 187, 493
BsrI ACTGG 2 cut(s) 49, 554
BssECI CCNNGG 2 cut(s) 28, 258
BssMI GATC 2 cut(s) 264, 377
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 159, 394
BstDEI CTNAG 2 cut(s) 321, 353
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 2 cut(s) 267, 380
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 264, 377
BstMWI GCNNNNNNNGC 2 cut(s) 235, 455
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 205
BstX2I RGATCY 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 1 cut(s) 79
BtsCI GGATG 1 cut(s) 169
BtsIMutI CAGTG 2 cut(s) 90, 399
Cfr13I GGNCC 2 cut(s) 185, 491
Csp6I GTAC 1 cut(s) 231
CviAII CATG 3 cut(s) 104, 190, 307
CviJI RGCY 6 cut(s) 79, 181, 218, 238, 512, 575
CviKI_1 RGCY 6 cut(s) 79, 181, 218, 238, 512, 575
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 2 cut(s) 321, 353
DpnI GATC 2 cut(s) 266, 379
DpnII GATC 2 cut(s) 264, 377
DraI TTTAAA 1 cut(s) 330
DraIII CACNNNGTG 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 185, 491
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 3 cut(s) 107, 193, 310
FaiI YATR 5 cut(s) 58, 105, 191, 308, 471
FaqI GGGAC 1 cut(s) 477
FatI CATG 3 cut(s) 103, 189, 306
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 219
HaeIII GGCC 1 cut(s) 79
HapII CCGG 2 cut(s) 64, 226
Hin1II CATG 3 cut(s) 107, 193, 310
HinfI GANTC 1 cut(s) 400
HpaII CCGG 2 cut(s) 64, 226
HphI GGTGA 2 cut(s) 131, 509
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 3 cut(s) 201, 322, 533
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 2 cut(s) 208, 269
HpyCH4III ACNGT 2 cut(s) 159, 394
HpyCH4V TGCA 2 cut(s) 170, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 235, 455
HpyF3I CTNAG 2 cut(s) 321, 353
Hsp92II CATG 3 cut(s) 107, 193, 310
Kzo9I GATC 2 cut(s) 264, 377
LpnPI CCDG 7 cut(s) 15, 30, 42, 77, 239, 407, 535
Lsp1109I GCAGC 1 cut(s) 205
MaeIII GTNAC 1 cut(s) 3
MalI GATC 2 cut(s) 266, 379
MboI GATC 2 cut(s) 264, 377
MboII GAAGA 2 cut(s) 387, 565
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 2 cut(s) 51, 444
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 2 cut(s) 146, 272
MseI TTAA 3 cut(s) 50, 204, 329
MslI CAYNNNNRTG 1 cut(s) 12
MspI CCGG 2 cut(s) 64, 226
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 235, 455
NdeII GATC 2 cut(s) 264, 377
NlaIII CATG 3 cut(s) 107, 193, 310
NlaIV GGNNCC 2 cut(s) 187, 493
NmuCI GTSAC 1 cut(s) 3
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 187, 493
PspPI GGNCC 2 cut(s) 185, 491
PsuI RGATCY 1 cut(s) 377
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 12
SaqAI TTAA 3 cut(s) 50, 204, 329
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 264, 377
Sau96I GGNCC 2 cut(s) 185, 491
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 103
SetI ASST 1 cut(s) 183
SinI GGWCC 2 cut(s) 185, 491
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 2 cut(s) 51, 444
SspI AATATT 1 cut(s) 196
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 2 cut(s) 159, 394
TaqI TCGA 1 cut(s) 453
TasI AATT 2 cut(s) 51, 444
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 3 cut(s) 50, 204, 329
Tru9I TTAA 3 cut(s) 50, 204, 329
TscAI CASTG 2 cut(s) 90, 399
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 28, 206, 288, 519
TspRI CASTG 2 cut(s) 90, 399
VpaK11BI GGWCC 2 cut(s) 185, 491
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.