Rh3BG262500

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001646
Physical Location & Seq
Forward (+)
25430361 .. 25435517
5157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG262500.1

Sequence Viewer

Length: 1032 bp
ATGGCCACTACTGCAGCTCCTGGTTCACACATGGCAGCAATTAAGCCTTGCATATCTTCTTCCCGAAGAACATACATGTCATGTACTGCCAATTTTGGTGCTAACAGTGAAGTGGCAGTTTCTAAGGGGCTTAGAAGCTCTTCCCACATCTCATCAAGGCAACCATTCTTCCCGAGCATCCAATCAGGCCCTGCGAAGTCTGTAAAGGTTATCACAAGAGCGATGTCAGCTGCAGCTGAGAACAGTCCATTGCCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGTGTGGCTGATGATCATGGATATGGATGGGCAATAGCAAAACAACTTGCTGCTGCTGGTGCTGAAATTCTTCTTGGTACATGGGTGCCGGCACTTAACATTTTCGAGTCCAGTTTAAGGCGTGGGAAGTTTGATGAATCACGCATATTGCCCGACGGTTCTTTGATGGAAATTACAAAAGTCTATCCATTGGATGCAGTTTTTGACAATCCTGATGATGTTCCTGAAGATATAAAATCAAACAAACGCTACTTGGGGTCCTCAAAGTGGACGGTTAAGGAGGTTGCTGAATCTGTTAAAGAGGATTTTGGCAGCATTGATATCCTAGTCCATTCCCTGGCTAATGGACCAGAGGTTGTCAAACCTCTTATGGAAACGTCAAGATATGGATATCTTGCAGCTATCTCTGCATCAAGCTACTCGTTTATTTCTTTACTCCAGCATTTTGCTCCAATAATGAACCCAGGTGGTGCGTCAATCTCTCTGACATACATTGCTTCCGAGAGGATCATTCCCGGATATGGTGGAGGTATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCTTTTGAAGCTGGGAGAAAACACAGAATCAGAGTCAACACAATATCTGCAGGCCCATTAAGAAGTCGTGCTGCAAAAGCTATTGGTTTTATCGATATGATGATTGATTACTCATCAGCCAATGCTCCCTTACCGAAAGAGTTATCTGCTGGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

343

Amino Acids

36.49

Weight (kDa)

9.28

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 190 - 338 2e-35 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 190 - 318 4.1e-08 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccB7I CCANNNNNTGG 1 cut(s) 634
AclWI GGATC 1 cut(s) 812
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 1 cut(s) 866
AfaI GTAC 2 cut(s) 85, 376
AfiI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
AflIII ACRYGT 3 cut(s) 75, 863, 865
AgsI TTSAA 1 cut(s) 881
AjnI CCWGG 4 cut(s) 19, 253, 633, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 8 cut(s) 17, 138, 230, 236, 698, 714, 884, 953
AluI AGCT 8 cut(s) 17, 138, 230, 236, 698, 714, 884, 953
AlwI GGATC 1 cut(s) 812
AlwNI CAGNNNCTG 2 cut(s) 20, 191
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 3 cut(s) 3, 187, 925
ApoI RAATTY 1 cut(s) 363
Asp700I GAANNNNTTC 2 cut(s) 139, 366
AspS9I GGNCC 4 cut(s) 188, 555, 644, 926
AsuC2I CCSGG 1 cut(s) 813
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 2 cut(s) 555, 644
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BbrPI CACGTG 1 cut(s) 866
BccI CCATC 2 cut(s) 318, 457
BciT130I CCWGG 4 cut(s) 21, 255, 635, 762
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 813
BfaI CTAG 2 cut(s) 623, 851
BfmI CTRYAG 3 cut(s) 12, 231, 921
Bme1390I CCNGG 5 cut(s) 21, 255, 635, 762, 813
Bme18I GGWCC 2 cut(s) 555, 644
BmeT110I CYCGRG 1 cut(s) 172
BmgT120I GGNCC 4 cut(s) 188, 555, 644, 926
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 5 cut(s) 21, 255, 635, 762, 813
BmsI GCATC 3 cut(s) 186, 481, 716
BpmI CTGGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 813
Bsa29I ATCGAT 1 cut(s) 966
BsaAI YACGTR 1 cut(s) 866
BsaJI CCNNGG 2 cut(s) 633, 760
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 3 cut(s) 248, 291, 789
BseBI CCWGG 4 cut(s) 21, 255, 635, 762
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 2 cut(s) 633, 760
BseGI GGATG 3 cut(s) 177, 329, 496
BseLI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
BseMI GCAATG 3 cut(s) 248, 291, 789
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 408
BseYI CCCAGC 1 cut(s) 884
BshFI GGCC 3 cut(s) 5, 189, 927
BshNI GGYRCC 1 cut(s) 382
BshVI ATCGAT 1 cut(s) 966
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 2 cut(s) 386, 813
BslI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 3 cut(s) 5, 189, 927
BsoBI CYCGRG 1 cut(s) 172
Bsp143I GATC 2 cut(s) 310, 804
BspANI GGCC 3 cut(s) 5, 189, 927
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 966
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 3 cut(s) 16, 235, 925
BspPI GGATC 1 cut(s) 812
BspQI GCTCTTC 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 3 cut(s) 248, 291, 789
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 633, 760
BssMI GATC 2 cut(s) 310, 804
Bst2UI CCWGG 4 cut(s) 21, 255, 635, 762
Bst4CI ACNGT 4 cut(s) 107, 245, 455, 571
Bst6I CTCTTC 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 387, 873, 925
BstDEI CTNAG 3 cut(s) 123, 131, 237
BstF5I GGATG 3 cut(s) 177, 329, 496
BstKTI GATC 2 cut(s) 313, 807
BstMBI GATC 2 cut(s) 310, 804
BstNI CCWGG 4 cut(s) 21, 255, 635, 762
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 5 cut(s) 19, 253, 633, 760, 811
BstSFI CTRYAG 3 cut(s) 12, 231, 921
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 3 cut(s) 5, 189, 927
BsuTUI ATCGAT 1 cut(s) 966
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 3 cut(s) 177, 329, 496
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 3 cut(s) 387, 873, 925
CaiI CAGNNNCTG 2 cut(s) 20, 191
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 4 cut(s) 188, 555, 644, 926
ClaI ATCGAT 1 cut(s) 966
CseI GACGC 1 cut(s) 759
Csp6I GTAC 2 cut(s) 84, 375
CviAII CATG 5 cut(s) 31, 76, 81, 314, 378
CviQI GTAC 2 cut(s) 84, 375
DdeI CTNAG 3 cut(s) 123, 131, 237
DpnI GATC 2 cut(s) 312, 806
DpnII GATC 2 cut(s) 310, 804
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco32I GATATC 2 cut(s) 619, 689
Eco47I GGWCC 2 cut(s) 555, 644
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 1 cut(s) 866
Eco88I CYCGRG 1 cut(s) 172
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoRII CCWGG 4 cut(s) 19, 253, 633, 760
EcoRV GATATC 2 cut(s) 619, 689
FaeI CATG 5 cut(s) 34, 79, 84, 317, 381
FatI CATG 5 cut(s) 30, 75, 80, 313, 377
FbaI TGATCA 1 cut(s) 310
FokI GGATG 3 cut(s) 164, 336, 503
FspBI CTAG 2 cut(s) 623, 851
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 3 cut(s) 5, 189, 927
HapII CCGG 2 cut(s) 386, 813
HgaI GACGC 1 cut(s) 759
Hin1II CATG 5 cut(s) 34, 79, 84, 317, 381
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 5 cut(s) 404, 434, 587, 900, 906
HpaII CCGG 2 cut(s) 386, 813
Hpy166II GTNNAC 3 cut(s) 26, 567, 910
Hpy188I TCNGA 3 cut(s) 783, 799, 905
Hpy188III TCNNGA 6 cut(s) 63, 172, 509, 521, 678, 851
Hpy8I GTNNAC 3 cut(s) 26, 567, 910
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 1 cut(s) 835
HpyCH4III ACNGT 4 cut(s) 107, 245, 455, 571
HpyCH4IV ACGT 2 cut(s) 674, 865
HpyCH4V TGCA 8 cut(s) 14, 51, 233, 494, 695, 707, 923, 947
HpyF3I CTNAG 3 cut(s) 123, 131, 237
HpySE526I ACGT 2 cut(s) 674, 865
Hsp92II CATG 5 cut(s) 34, 79, 84, 317, 381
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 310, 804
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 3 cut(s) 22, 751, 1003
LweI GCATC 3 cut(s) 186, 481, 716
MaeI CTAG 2 cut(s) 623, 851
MaeII ACGT 2 cut(s) 674, 865
MaeIII GTNAC 1 cut(s) 857
MalI GATC 2 cut(s) 312, 806
MboI GATC 2 cut(s) 310, 804
MboII GAAGA 7 cut(s) 48, 51, 78, 132, 160, 359, 536
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 39, 91, 363, 468
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 413, 915
MnlI CCTC 8 cut(s) 269, 568, 571, 592, 643, 672, 795, 818
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MroXI GAANNNNTTC 2 cut(s) 139, 366
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
MslI CAYNNNNRTG 2 cut(s) 318, 866
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 230, 236
MspI CCGG 2 cut(s) 386, 813
MspR9I CCNGG 5 cut(s) 21, 255, 635, 762, 813
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 4 cut(s) 21, 255, 635, 762
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 813
NdeII GATC 2 cut(s) 310, 804
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 34, 79, 84, 317, 381
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 857
NspI RCATGY 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 866
PciI ACATGT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 145
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PdmI GAANNNNTTC 2 cut(s) 139, 366
PfeI GAWTC 3 cut(s) 434, 587, 900
PflMI CCANNNNNTGG 1 cut(s) 634
PfoI TCCNGGA 1 cut(s) 811
PleI GAGTC 2 cut(s) 412, 914
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
PpsI GAGTC 2 cut(s) 412, 914
Ppu21I YACGTR 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 555
PscI ACATGT 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 4 cut(s) 19, 253, 633, 760
PspCI CACGTG 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 4 cut(s) 19, 253, 633, 760
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 4 cut(s) 188, 555, 644, 926
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 3 cut(s) 16, 235, 925
PstNI CAGNNNCTG 2 cut(s) 20, 191
PvuII CAGCTG 2 cut(s) 230, 236
RsaI GTAC 2 cut(s) 85, 376
RsaNI GTAC 2 cut(s) 84, 375
RseI CAYNNNNRTG 2 cut(s) 318, 866
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
Sau3AI GATC 2 cut(s) 310, 804
Sau96I GGNCC 4 cut(s) 188, 555, 644, 926
SchI GAGTC 2 cut(s) 413, 915
ScrFI CCNGG 5 cut(s) 21, 255, 635, 762, 813
SfaNI GCATC 3 cut(s) 186, 481, 716
SfcI CTRYAG 3 cut(s) 12, 231, 921
SinI GGWCC 2 cut(s) 555, 644
SmiMI CAYNNNNRTG 2 cut(s) 318, 866
Sse9I AATT 4 cut(s) 39, 91, 363, 468
SspMI CTAG 2 cut(s) 623, 851
StyD4I CCNGG 5 cut(s) 19, 253, 633, 760, 811
TaaI ACNGT 4 cut(s) 107, 245, 455, 571
TaiI ACGT 2 cut(s) 677, 868
TaqI TCGA 2 cut(s) 402, 966
TasI AATT 4 cut(s) 39, 91, 363, 468
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 3 cut(s) 434, 587, 900
Tru1I TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
Tru9I TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 857
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 3 cut(s) 280, 447, 770
TspRI CASTG 1 cut(s) 112
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 555, 644
XapI RAATTY 1 cut(s) 363
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 1 cut(s) 79
XmnI GAANNNNTTC 2 cut(s) 139, 366
XspI CTAG 2 cut(s) 623, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.