Rmu_sc0004329.1_g000006

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004329.1
OG0001646
Physical Location & Seq
Reverse (-)
15342 .. 19801
4460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004329.1_g000006.1.cds

Sequence Viewer

Length: 1221 bp
atgggaatggggcatattagagctgaacccttccaatcaagtccaaaggtccagttaagcacaagggcaaacccgtcattacacaggggcgccaaaagcaacgaacagatcaacgctctgtttcggcatttacaaagtttgccaacaccaactgaccaaccctccaacaaaccaaaagcctcaggctttggtttcatcgaacccccacacaaaaccacactccctcacatttatataaaaacccctcactgcaacccttccttctttgctctctcaaacactatcagatttcgaagagttttgttttggtttctggtgatctgtatcgaaaatggccactactgcagctcctgtgaagtggcagtttctaaggggcttagaagctcttcccacatctcatcaaggcaaccattcttccggagcatccaatcaggctctgcgaagtctgtaaaggttatcacaagagcgatgtcagcagcagctgagaacactccattgcctggattgcctattgatttgagagtctatccattggatgcagtttttgacaatcctgatgatgttcctgaagatataaaatcaaacaaacgctacttggggtcctcaaagtggacggttaaggaggttgctgaatctgttaaggaggattttggcagcattgatatcctagtccattccctggctaatggaccagaggttgtcaaacctcttatggaaacatcaagatatggatatcttgcagctatctctgcatcaagctactcgtttatttctttactccagcattttgctccaataatgaacccaggtggtgcgtcaatctctctgacatacattgcttccgagaggatcattcccggatatggtggaggtatgagttcagcgaaggctgctctagagagtgacacacgtgtgctggcctttgaagctgggagaaaacacagactcagagtcaacacaatatctgcaggcccattaagaagtcgtgctgcaaaagctattggttttatcgatatgatgattgattactcatcagccaatgctcccttaccgaaagagttgtctgctgaggaggtggggaacgttgctgccttccttgcatcaccattggcatcagcaatcaccggcagtgttgtatacgtcgacaatggtctaaatgcaatgggtgtgggagttgacagcccaatttttgaaaaccttgagcttccaaaagaagcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

406

Amino Acids

43.88

Weight (kDa)

9.02

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccB7I CCANNNNNTGG 2 cut(s) 500, 679
AccI GTMKAC 2 cut(s) 1137, 1143
AccIII TCCGGA 1 cut(s) 417
AclI AACGTT 1 cut(s) 1083
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 588
AcvI CACGTG 1 cut(s) 911
AcyI GRCGYC 1 cut(s) 90
AfiI CCNNNNNNNGG 4 cut(s) 500, 609, 679, 863
AflIII ACRYGT 2 cut(s) 908, 910
AgsI TTSAA 2 cut(s) 926, 1193
AhdI GACNNNNNGTC 1 cut(s) 950
AjnI CCWGG 3 cut(s) 499, 678, 805
AleI CACNNNNGTG 1 cut(s) 911
AluBI AGCT 9 cut(s) 23, 348, 384, 482, 743, 759, 929, 998, 1204
AluI AGCT 9 cut(s) 23, 348, 384, 482, 743, 759, 929, 998, 1204
AlwI GGATC 1 cut(s) 857
AlwNI CAGNNNCTG 3 cut(s) 351, 437, 482
Aor13HI TCCGGA 1 cut(s) 417
AoxI GGCC 3 cut(s) 334, 918, 970
ApeKI GCWGC 8 cut(s) 345, 476, 479, 654, 740, 890, 989, 1088
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 4 cut(s) 49, 600, 689, 971
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 3 cut(s) 328, 1095, 1114
AsuII TTCGAA 1 cut(s) 292
AvaII GGWCC 3 cut(s) 49, 600, 689
AxyI CCTNAGG 1 cut(s) 181
BalI TGGCCA 1 cut(s) 336
BanI GGYRCC 1 cut(s) 89
BbrPI CACGTG 1 cut(s) 911
BbvCI CCTCAGC 1 cut(s) 1068
BbvI GCAGC 8 cut(s) 357, 488, 491, 666, 752, 877, 976, 1075
BciT130I CCWGG 3 cut(s) 501, 680, 807
BcnI CCSGG 1 cut(s) 858
BfaI CTAG 3 cut(s) 668, 896, 1219
BfmI CTRYAG 2 cut(s) 343, 966
BfoI RGCGCY 1 cut(s) 93
BisI GCNGC 8 cut(s) 346, 477, 480, 655, 741, 891, 990, 1089
BlsI GCNGC 8 cut(s) 347, 478, 481, 656, 742, 892, 991, 1090
Bme1390I CCNGG 4 cut(s) 501, 680, 807, 858
Bme18I GGWCC 3 cut(s) 49, 600, 689
BmeRI GACNNNNNGTC 1 cut(s) 950
BmgT120I GGNCC 4 cut(s) 49, 600, 689, 971
BmiI GGNNCC 2 cut(s) 91, 601
BmrFI CCNGG 4 cut(s) 501, 680, 807, 858
BmsI GCATC 5 cut(s) 432, 526, 761, 1109, 1121
BoxI GACNNNNGTC 1 cut(s) 1149
BpmI CTGGAG 1 cut(s) 764
Bpu10I CCTNAGC 1 cut(s) 1068
Bpu14I TTCGAA 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 1220
BpuMI CCSGG 1 cut(s) 858
Bsa29I ATCGAT 1 cut(s) 1011
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 678, 805
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 332, 362
Bsc4I CCNNNNNNNGG 4 cut(s) 500, 609, 679, 863
Bse118I RCCGGY 1 cut(s) 1124
Bse1I ACTGG 1 cut(s) 52
Bse21I CCTNAGG 1 cut(s) 181
Bse3DI GCAATG 3 cut(s) 494, 834, 1167
Bse8I GATNNNNATC 1 cut(s) 323
BseAI TCCGGA 1 cut(s) 417
BseBI CCWGG 3 cut(s) 501, 680, 807
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 2 cut(s) 678, 805
BseGI GGATG 2 cut(s) 423, 541
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 4 cut(s) 500, 609, 679, 863
BseMI GCAATG 3 cut(s) 494, 834, 1167
BseMII CTCAG 4 cut(s) 195, 474, 961, 1059
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 1 cut(s) 1085
BseXI GCAGC 8 cut(s) 357, 488, 491, 666, 752, 877, 976, 1075
BseYI CCCAGC 1 cut(s) 929
BshFI GGCC 3 cut(s) 336, 920, 972
BshNI GGYRCC 1 cut(s) 89
BshVI ATCGAT 1 cut(s) 1011
BsiSI CCGG 3 cut(s) 418, 858, 1125
BslI CCNNNNNNNGG 4 cut(s) 500, 609, 679, 863
BsnI GGCC 3 cut(s) 336, 920, 972
Bsp119I TTCGAA 1 cut(s) 292
Bsp13I TCCGGA 1 cut(s) 417
Bsp143I GATC 3 cut(s) 108, 318, 849
BspANI GGCC 3 cut(s) 336, 920, 972
BspCNI CTCAG 4 cut(s) 194, 475, 960, 1060
BspDI ATCGAT 1 cut(s) 1011
BspEI TCCGGA 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 91, 601
BspMAI CTGCAG 2 cut(s) 347, 970
BspPI GGATC 1 cut(s) 857
BspQI GCTCTTC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 494, 834, 1167
BsrFI RCCGGY 1 cut(s) 1124
BsrI ACTGG 1 cut(s) 52
BssAI RCCGGY 1 cut(s) 1124
BssECI CCNNGG 2 cut(s) 678, 805
BssMI GATC 3 cut(s) 108, 318, 849
BssNAI GTATAC 1 cut(s) 1138
BssNI GRCGYC 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 1138
Bst2UI CCWGG 3 cut(s) 501, 680, 807
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 2 cut(s) 289, 391
BstACI GRCGYC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 911
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 918, 970
BstDEI CTNAG 6 cut(s) 181, 369, 377, 483, 947, 1068
BstF5I GGATG 2 cut(s) 423, 541
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 3 cut(s) 111, 321, 852
BstMBI GATC 3 cut(s) 108, 318, 849
BstMWI GCNNNNNNNGC 9 cut(s) 96, 342, 473, 505, 749, 890, 926, 995, 1097
BstNI CCWGG 3 cut(s) 501, 680, 807
BstPAI GACNNNNGTC 1 cut(s) 1149
BstSCI CCNGG 4 cut(s) 499, 678, 805, 856
BstSFI CTRYAG 2 cut(s) 343, 966
BstV1I GCAGC 8 cut(s) 357, 488, 491, 666, 752, 877, 976, 1075
BstZ17I GTATAC 1 cut(s) 1138
Bsu15I ATCGAT 1 cut(s) 1011
Bsu36I CCTNAGG 1 cut(s) 181
BsuRI GGCC 3 cut(s) 336, 920, 972
BsuTUI ATCGAT 1 cut(s) 1011
BtgZI GCGATG 1 cut(s) 482
BtsCI GGATG 2 cut(s) 423, 541
BtsI GCAGTG 2 cut(s) 247, 1135
BtsIMutI CAGTG 2 cut(s) 247, 1135
Cac8I GCNNGC 2 cut(s) 918, 970
CaiI CAGNNNCTG 3 cut(s) 351, 437, 482
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 1124
Cfr13I GGNCC 4 cut(s) 49, 600, 689, 971
ClaI ATCGAT 1 cut(s) 1011
CseI GACGC 1 cut(s) 804
CspCI CAANNNNNGTGG 2 cut(s) 1149, 1184
DdeI CTNAG 6 cut(s) 181, 369, 377, 483, 947, 1068
DinI GGCGCC 1 cut(s) 91
DpnI GATC 3 cut(s) 110, 320, 851
DpnII GATC 3 cut(s) 108, 318, 849
DriI GACNNNNNGTC 1 cut(s) 950
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 2 cut(s) 289, 391
Eam1105I GACNNNNNGTC 1 cut(s) 950
EarI CTCTTC 2 cut(s) 289, 391
Eco32I GATATC 2 cut(s) 664, 734
Eco47I GGWCC 3 cut(s) 49, 600, 689
Eco57I CTGAAG 1 cut(s) 588
Eco72I CACGTG 1 cut(s) 911
Eco81I CCTNAGG 1 cut(s) 181
EcoO109I RGGNCCY 1 cut(s) 600
EcoRII CCWGG 3 cut(s) 499, 678, 805
EcoRV GATATC 2 cut(s) 664, 734
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
FblI GTMKAC 2 cut(s) 1137, 1143
Fnu4HI GCNGC 8 cut(s) 346, 477, 480, 655, 741, 891, 990, 1089
FokI GGATG 2 cut(s) 410, 548
Fsp4HI GCNGC 8 cut(s) 346, 477, 480, 655, 741, 891, 990, 1089
FspBI CTAG 3 cut(s) 668, 896, 1219
GlaI GCGC 1 cut(s) 91
GluI GCNGC 8 cut(s) 346, 477, 480, 655, 741, 891, 990, 1089
GsaI CCCAGC 1 cut(s) 933
GsuI CTGGAG 1 cut(s) 764
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 3 cut(s) 336, 920, 972
HapII CCGG 3 cut(s) 418, 858, 1125
HgaI GACGC 1 cut(s) 804
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 90
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 3 cut(s) 955, 1144, 1177
HindII GTYRAC 3 cut(s) 955, 1144, 1177
HinfI GANTC 4 cut(s) 522, 632, 945, 951
HpaII CCGG 3 cut(s) 418, 858, 1125
HphI GGTGA 3 cut(s) 328, 1095, 1114
Hpy166II GTNNAC 5 cut(s) 612, 955, 1138, 1144, 1177
Hpy188I TCNGA 4 cut(s) 287, 828, 844, 950
Hpy188III TCNNGA 5 cut(s) 418, 554, 566, 723, 896
Hpy8I GTNNAC 5 cut(s) 612, 955, 1138, 1144, 1177
Hpy99I CGWCG 1 cut(s) 1145
HpyAV CCTTC 5 cut(s) 40, 267, 271, 880, 1102
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 3 cut(s) 910, 1083, 1140
HpyCH4V TGCA 9 cut(s) 252, 345, 539, 740, 752, 968, 992, 1100, 1160
HpyF10VI GCNNNNNNNGC 9 cut(s) 96, 342, 473, 505, 749, 890, 926, 995, 1097
HpyF3I CTNAG 6 cut(s) 181, 369, 377, 483, 947, 1068
HpySE526I ACGT 3 cut(s) 910, 1083, 1140
Hsp92I GRCGYC 1 cut(s) 90
HspAI GCGC 1 cut(s) 90
KasI GGCGCC 1 cut(s) 89
Kpn2I TCCGGA 1 cut(s) 417
Kzo9I GATC 3 cut(s) 108, 318, 849
LguI GCTCTTC 1 cut(s) 391
LmnI GCTCC 4 cut(s) 353, 420, 796, 1048
Lsp1109I GCAGC 8 cut(s) 357, 488, 491, 666, 752, 877, 976, 1075
LweI GCATC 5 cut(s) 432, 526, 761, 1109, 1121
MaeI CTAG 3 cut(s) 668, 896, 1219
MaeII ACGT 3 cut(s) 910, 1083, 1140
MaeIII GTNAC 1 cut(s) 902
MalI GATC 3 cut(s) 110, 320, 851
MboI GATC 3 cut(s) 108, 318, 849
MboII GAAGA 4 cut(s) 306, 378, 406, 581
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 1 cut(s) 1185
MluNI TGGCCA 1 cut(s) 336
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 3 cut(s) 531, 939, 960
MmeI TCCRAC 1 cut(s) 189
Mox20I TGGCCA 1 cut(s) 336
MroI TCCGGA 1 cut(s) 417
MroXI GAANNNNTTC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 336
MseI TTAA 4 cut(s) 56, 618, 639, 977
MslI CAYNNNNRTG 1 cut(s) 911
Msp20I TGGCCA 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 3 cut(s) 418, 858, 1125
MspR9I CCNGG 4 cut(s) 501, 680, 807, 858
MvaI CCWGG 3 cut(s) 501, 680, 807
MwoI GCNNNNNNNGC 9 cut(s) 96, 342, 473, 505, 749, 890, 926, 995, 1097
NarI GGCGCC 1 cut(s) 90
NciI CCSGG 1 cut(s) 858
NdeII GATC 3 cut(s) 108, 318, 849
NlaIV GGNNCC 2 cut(s) 91, 601
NmuCI GTSAC 1 cut(s) 902
NspV TTCGAA 1 cut(s) 292
OliI CACNNNNGTG 1 cut(s) 911
PciSI GCTCTTC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 632
PflMI CCANNNNNTGG 2 cut(s) 500, 679
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 8 cut(s) 347, 478, 481, 656, 742, 892, 991, 1090
PleI GAGTC 3 cut(s) 530, 939, 959
PluTI GGCGCC 1 cut(s) 93
PmaCI CACGTG 1 cut(s) 911
PmlI CACGTG 1 cut(s) 911
PpsI GAGTC 3 cut(s) 530, 939, 959
Ppu21I YACGTR 1 cut(s) 911
PpuMI RGGWCCY 1 cut(s) 600
PshAI GACNNNNGTC 1 cut(s) 1149
Psp1406I AACGTT 1 cut(s) 1083
Psp5II RGGWCCY 1 cut(s) 600
Psp6I CCWGG 3 cut(s) 499, 678, 805
PspCI CACGTG 1 cut(s) 911
PspFI CCCAGC 1 cut(s) 929
PspGI CCWGG 3 cut(s) 499, 678, 805
PspN4I GGNNCC 2 cut(s) 91, 601
PspPI GGNCC 4 cut(s) 49, 600, 689, 971
PspPPI RGGWCCY 1 cut(s) 600
PstI CTGCAG 2 cut(s) 347, 970
PstNI CAGNNNCTG 3 cut(s) 351, 437, 482
PvuII CAGCTG 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 911
SalI GTCGAC 1 cut(s) 1142
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 4 cut(s) 56, 618, 639, 977
SatI GCNGC 8 cut(s) 346, 477, 480, 655, 741, 891, 990, 1089
Sau3AI GATC 3 cut(s) 108, 318, 849
Sau96I GGNCC 4 cut(s) 49, 600, 689, 971
SchI GAGTC 3 cut(s) 531, 939, 960
ScrFI CCNGG 4 cut(s) 501, 680, 807, 858
SfaNI GCATC 5 cut(s) 432, 526, 761, 1109, 1121
SfcI CTRYAG 2 cut(s) 343, 966
SfoI GGCGCC 1 cut(s) 91
SfuI TTCGAA 1 cut(s) 292
SinI GGWCC 3 cut(s) 49, 600, 689
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
Sse9I AATT 1 cut(s) 1185
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 3 cut(s) 668, 896, 1219
StyD4I CCNGG 4 cut(s) 499, 678, 805, 856
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 3 cut(s) 913, 1086, 1143
TaqI TCGA 5 cut(s) 198, 292, 327, 1011, 1143
TasI AATT 1 cut(s) 1185
TfiI GAWTC 1 cut(s) 632
Tru1I TTAA 4 cut(s) 56, 618, 639, 977
Tru9I TTAA 4 cut(s) 56, 618, 639, 977
TscAI CASTG 2 cut(s) 254, 1135
TseFI GTSAC 1 cut(s) 902
TseI GCWGC 8 cut(s) 345, 476, 479, 654, 740, 890, 989, 1088
Tsp45I GTSAC 1 cut(s) 902
TspDTI ATGAA 2 cut(s) 184, 815
TspRI CASTG 2 cut(s) 254, 1135
Van91I CCANNNNNTGG 2 cut(s) 500, 679
VpaK11BI GGWCC 3 cut(s) 49, 600, 689
XbaI TCTAGA 1 cut(s) 895
XmiI GTMKAC 2 cut(s) 1137, 1143
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 3 cut(s) 668, 896, 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.