pycom12g05150

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001646
Physical Location & Seq
Forward (+)
5031285 .. 5034567
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05150.8

Sequence Viewer

Length: 1179 bp
ATGGCCACGACTGTAGCACCCGGATCACACATGGCAGCGGTGAAGCCTTGCATGTCGTCCTCTCAAGGAACTTATATGTCATGCACTGCAAAGTTTGGTACAAACAGCGAGGTGGGAGTTTGGAGAAGGCTTAAGAGTTCTTCTCACATCTCAGCAACACGGTCATTCTCCCAGAGCTTGAAATCAGGGCCTGTGAAATCCGTAGAGGTTGTCACAAGAGCAATGTCAGGAGTAGCTGAGAACACTCCACTGTCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATAGCTGGTGTGGCTGATGACCATGGATATGGATGGGCAATATCAAAAGCTCTTGCTGCTGCGGGAGCTGAAATTCTAGTTGGTACATGGGTGCCGGCACTTAACATTTTTGAATCCAGTTTAAGGCGTGGAAAGTTTGATGAGTCACGCACACTGCCAGATGGCTCTTTGATGGAAATTACCAAGGTTTACCCATTGGATGCAGTTTTTGACAGTCCCGAGGATGTTCCTGAAGATATAAAAACCAATAAACGCTACTTGGGGTCCTCAAATTGGACTGTTAAGGAGGTGGTTGAATCTGTAAAGCAGGATTTTGGCAGCATTGACATCCTTGTCCACTCACTTGCCAATGGACCAGAGGTTACCAAACCTCTTTTGGAGACGTCAAGATATGGATATCTTGCAGCCATCTCTGCATCAAGTTACTCATATATTTCTTTACTCAAGCATTTTGCTCCAATAATGAACCCAGGTGGTGCTTCACTCTCTCTAACATACATTGCTTCAGAAAGGATCATTCCAGGATATGGTGGAGGCATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGAAGAAAGCACAATATTAGAGTCAACACAATATCTGCAGGACCATTAAGAAGTCGTGCGGCAAAGGCAATTGGATTCATTGATATGATGATTGATTACTCATCGGAAAATGCACCCTTGCAGAAGGAATTATCTGCAGAGGAGGTGGGGAACGCTGCTGCCTTCCTAGCATCGCCATTGGCATCAGCCATCACCGGGACTGTCCTATACGTCGACAATGGCCTGAATGCGATGGGAGTTGGAGTCGACAGCCCAGTTTTTGAAAACCTTGATATTCCAAAGGCCGCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

393

Amino Acids

41.48

Weight (kDa)

7.02

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 623
AatII GACGTC 1 cut(s) 677
AccB1I GGYRCC 1 cut(s) 382
AccB7I CCANNNNNTGG 1 cut(s) 818
AccI GTMKAC 2 cut(s) 1098, 1131
AciI CCGC 4 cut(s) 38, 353, 944, 1170
AclWI GGATC 2 cut(s) 31, 812
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 2 cut(s) 543, 780
AcvI CACGTG 1 cut(s) 866
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 2 cut(s) 100, 376
AfiI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 818, 1080
AflII CTTAAG 1 cut(s) 131
AflIII ACRYGT 2 cut(s) 863, 865
AgsI TTSAA 5 cut(s) 181, 404, 587, 881, 1148
AjnI CCWGG 2 cut(s) 760, 811
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 6 cut(s) 177, 236, 296, 341, 359, 884
AluI AGCT 6 cut(s) 177, 236, 296, 341, 359, 884
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 2 cut(s) 31, 812
AlwNI CAGNNNCTG 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 5 cut(s) 3, 188, 873, 1105, 1167
ApeKI GCWGC 8 cut(s) 35, 347, 350, 609, 695, 845, 1040, 1043
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 4 cut(s) 188, 555, 644, 926
AsuC2I CCSGG 2 cut(s) 21, 1081
AsuHPI GGTGA 2 cut(s) 52, 1069
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 3 cut(s) 555, 644, 926
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BbrPI CACGTG 1 cut(s) 866
BbvI GCAGC 8 cut(s) 47, 334, 337, 621, 707, 832, 1027, 1030
BccI CCATC 6 cut(s) 318, 446, 457, 707, 1082, 1111
BciT130I CCWGG 2 cut(s) 762, 813
BcnI CCSGG 2 cut(s) 21, 1081
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 3 cut(s) 368, 851, 1052
BfmI CTRYAG 3 cut(s) 12, 921, 1020
BfrI CTTAAG 1 cut(s) 131
Bme1390I CCNGG 4 cut(s) 21, 762, 813, 1081
Bme18I GGWCC 3 cut(s) 555, 644, 926
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 4 cut(s) 188, 555, 644, 926
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 4 cut(s) 21, 762, 813, 1081
BmrI ACTGGG 1 cut(s) 1133
BmsI GCATC 4 cut(s) 481, 716, 1064, 1076
BmuI ACTGGG 1 cut(s) 1133
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpuEI CTTGAG 2 cut(s) 48, 719
BpuMI CCSGG 2 cut(s) 21, 1081
BsaAI YACGTR 1 cut(s) 866
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 4 cut(s) 313, 474, 510, 760
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 414, 564, 818, 1080
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 2 cut(s) 408, 1139
Bse3DI GCAATG 2 cut(s) 228, 789
BseBI CCWGG 2 cut(s) 762, 813
BseDI CCNNGG 4 cut(s) 313, 474, 510, 760
BseGI GGATG 4 cut(s) 329, 496, 520, 618
BseLI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 818, 1080
BseMI GCAATG 2 cut(s) 228, 789
BseMII CTCAG 2 cut(s) 165, 228
BseNI ACTGG 2 cut(s) 408, 1139
BseRI GAGGAG 1 cut(s) 1040
BseXI GCAGC 8 cut(s) 47, 334, 337, 621, 707, 832, 1027, 1030
BshFI GGCC 5 cut(s) 5, 190, 875, 1107, 1169
BshNI GGYRCC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 3 cut(s) 21, 386, 1080
BslFI GGGAC 2 cut(s) 492, 1096
BslI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 818, 1080
BsmAI GTCTC 1 cut(s) 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 2 cut(s) 492, 1096
BsmI GAATGC 2 cut(s) 287, 1117
BsnI GGCC 5 cut(s) 5, 190, 875, 1107, 1169
BsoBI CYCGRG 1 cut(s) 509
Bsp143I GATC 2 cut(s) 23, 804
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 4 cut(s) 38, 353, 944, 1170
BspANI GGCC 5 cut(s) 5, 190, 875, 1107, 1169
BspCNI CTCAG 2 cut(s) 164, 229
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 2 cut(s) 925, 1024
BspPI GGATC 2 cut(s) 31, 812
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 228, 789
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 2 cut(s) 408, 1139
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 4 cut(s) 313, 474, 510, 760
BssMI GATC 2 cut(s) 23, 804
BssNI GRCGYC 1 cut(s) 674
BssT1I CCWWGG 2 cut(s) 313, 474
Bst2UI CCWGG 2 cut(s) 762, 813
Bst4CI ACNGT 6 cut(s) 13, 162, 252, 506, 571, 1087
BstACI GRCGYC 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 131
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 2 cut(s) 387, 873
BstDEI CTNAG 2 cut(s) 151, 237
BstDSI CCRYGG 1 cut(s) 313
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 4 cut(s) 329, 496, 520, 618
BstKTI GATC 2 cut(s) 26, 807
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 2 cut(s) 23, 804
BstMWI GCNNNNNNNGC 9 cut(s) 293, 302, 347, 356, 704, 845, 881, 950, 1052
BstNI CCWGG 2 cut(s) 762, 813
BstNSI RCATGY 1 cut(s) 55
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 4 cut(s) 19, 760, 811, 1079
BstSFI CTRYAG 3 cut(s) 12, 921, 1020
BstV1I GCAGC 8 cut(s) 47, 334, 337, 621, 707, 832, 1027, 1030
BstXI CCANNNNNNTGG 1 cut(s) 320
BsuRI GGCC 5 cut(s) 5, 190, 875, 1107, 1169
BtgI CCRYGG 1 cut(s) 313
BtgZI GCGATG 2 cut(s) 1041, 1130
BtsCI GGATG 4 cut(s) 329, 496, 520, 618
BtsI GCAGTG 2 cut(s) 84, 443
BtsIMutI CAGTG 3 cut(s) 84, 248, 443
Cac8I GCNNGC 2 cut(s) 387, 873
CaiI CAGNNNCTG 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 4 cut(s) 188, 555, 644, 926
Csp6I GTAC 2 cut(s) 99, 375
CviAII CATG 6 cut(s) 31, 52, 81, 314, 378, 829
CviQI GTAC 2 cut(s) 99, 375
DdeI CTNAG 2 cut(s) 151, 237
DpnI GATC 2 cut(s) 25, 806
DpnII GATC 2 cut(s) 23, 804
DrdI GACNNNNNNGTC 1 cut(s) 623
DseDI GACNNNNNNGTC 1 cut(s) 623
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 313, 474
Eco32I GATATC 1 cut(s) 689
Eco47I GGWCC 3 cut(s) 555, 644, 926
Eco57I CTGAAG 2 cut(s) 543, 780
Eco72I CACGTG 1 cut(s) 866
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 652
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 760, 811
EcoRV GATATC 1 cut(s) 689
EcoT14I CCWWGG 2 cut(s) 313, 474
ErhI CCWWGG 2 cut(s) 313, 474
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 6 cut(s) 34, 55, 84, 317, 381, 832
FaqI GGGAC 2 cut(s) 492, 1096
FatI CATG 6 cut(s) 30, 51, 80, 313, 377, 828
FauI CCCGC 1 cut(s) 346
FblI GTMKAC 2 cut(s) 1098, 1131
FokI GGATG 4 cut(s) 336, 503, 527, 605
FspBI CTAG 3 cut(s) 368, 851, 1052
HaeIII GGCC 5 cut(s) 5, 190, 875, 1107, 1169
HapII CCGG 3 cut(s) 21, 386, 1080
Hin1I GRCGYC 1 cut(s) 674
Hin1II CATG 6 cut(s) 34, 55, 84, 317, 381, 832
HincII GTYRAC 3 cut(s) 910, 1099, 1132
HindII GTYRAC 3 cut(s) 910, 1099, 1132
HinfI GANTC 6 cut(s) 404, 434, 587, 906, 960, 1128
HpaII CCGG 3 cut(s) 21, 386, 1080
HphI GGTGA 2 cut(s) 52, 1069
Hpy166II GTNNAC 5 cut(s) 481, 628, 910, 1099, 1132
Hpy188I TCNGA 2 cut(s) 799, 991
Hpy188III TCNNGA 6 cut(s) 228, 255, 509, 521, 678, 851
Hpy8I GTNNAC 5 cut(s) 481, 628, 910, 1099, 1132
Hpy99I CGWCG 1 cut(s) 1100
HpyAV CCTTC 4 cut(s) 120, 835, 1003, 1057
HpyCH4III ACNGT 6 cut(s) 13, 162, 252, 506, 571, 1087
HpyCH4IV ACGT 3 cut(s) 674, 865, 1095
HpyF10VI GCNNNNNNNGC 9 cut(s) 293, 302, 347, 356, 704, 845, 881, 950, 1052
HpyF3I CTNAG 2 cut(s) 151, 237
HpySE526I ACGT 3 cut(s) 674, 865, 1095
Hsp92I GRCGYC 1 cut(s) 674
Hsp92II CATG 6 cut(s) 34, 55, 84, 317, 381, 832
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 2 cut(s) 23, 804
LmnI GCTCC 2 cut(s) 356, 751
Lsp1109I GCAGC 8 cut(s) 47, 334, 337, 621, 707, 832, 1027, 1030
LweI GCATC 4 cut(s) 481, 716, 1064, 1076
MaeI CTAG 3 cut(s) 368, 851, 1052
MaeII ACGT 3 cut(s) 674, 865, 1095
MaeIII GTNAC 5 cut(s) 211, 435, 652, 713, 857
MalI GATC 2 cut(s) 25, 806
MboI GATC 2 cut(s) 23, 804
MboII GAAGA 3 cut(s) 132, 536, 900
MfeI CAATTG 1 cut(s) 954
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 363, 468, 562, 954, 1013
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 443, 915, 1137
MmeI TCCRAC 1 cut(s) 1105
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 132, 393, 413, 573, 932
MslI CAYNNNNRTG 3 cut(s) 318, 866, 968
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 38
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 3 cut(s) 21, 386, 1080
MspR9I CCNGG 4 cut(s) 21, 762, 813, 1081
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 2 cut(s) 287, 1117
MvaI CCWGG 2 cut(s) 762, 813
MwoI GCNNNNNNNGC 9 cut(s) 293, 302, 347, 356, 704, 845, 881, 950, 1052
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 2 cut(s) 21, 1081
NcoI CCATGG 1 cut(s) 313
NdeII GATC 2 cut(s) 23, 804
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 6 cut(s) 34, 55, 84, 317, 381, 832
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 3 cut(s) 211, 435, 857
NspI RCATGY 1 cut(s) 55
OliI CACNNNNGTG 1 cut(s) 866
PctI GAATGC 2 cut(s) 287, 1117
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 3 cut(s) 404, 587, 960
PflMI CCANNNNNTGG 1 cut(s) 818
PfoI TCCNGGA 1 cut(s) 811
PleI GAGTC 3 cut(s) 442, 914, 1136
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
PpsI GAGTC 3 cut(s) 442, 914, 1136
Ppu21I YACGTR 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 760, 811
PspCI CACGTG 1 cut(s) 866
PspEI GGTNACC 1 cut(s) 652
PspGI CCWGG 2 cut(s) 760, 811
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 4 cut(s) 188, 555, 644, 926
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 2 cut(s) 925, 1024
PstNI CAGNNNCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 100, 376
RsaNI GTAC 2 cut(s) 99, 375
RseI CAYNNNNRTG 3 cut(s) 318, 866, 968
SalI GTCGAC 2 cut(s) 1097, 1130
SaqAI TTAA 5 cut(s) 132, 393, 413, 573, 932
Sau3AI GATC 2 cut(s) 23, 804
Sau96I GGNCC 4 cut(s) 188, 555, 644, 926
SchI GAGTC 3 cut(s) 443, 915, 1137
ScrFI CCNGG 4 cut(s) 21, 762, 813, 1081
SfaNI GCATC 4 cut(s) 481, 716, 1064, 1076
SfcI CTRYAG 3 cut(s) 12, 921, 1020
SinI GGWCC 3 cut(s) 555, 644, 926
SmiMI CAYNNNNRTG 3 cut(s) 318, 866, 968
SmlI CTYRAG 3 cut(s) 63, 131, 734
SmoI CTYRAG 3 cut(s) 63, 131, 734
Sse9I AATT 5 cut(s) 363, 468, 562, 954, 1013
SsiI CCGC 4 cut(s) 38, 353, 944, 1170
SspI AATATT 1 cut(s) 901
SspMI CTAG 3 cut(s) 368, 851, 1052
StyD4I CCNGG 4 cut(s) 19, 760, 811, 1079
StyI CCWWGG 2 cut(s) 313, 474
TaaI ACNGT 6 cut(s) 13, 162, 252, 506, 571, 1087
TaiI ACGT 3 cut(s) 677, 868, 1098
TaqI TCGA 2 cut(s) 1098, 1131
TasI AATT 5 cut(s) 363, 468, 562, 954, 1013
TauI GCSGC 2 cut(s) 947, 1172
TfiI GAWTC 3 cut(s) 404, 587, 960
Tru1I TTAA 5 cut(s) 132, 393, 413, 573, 932
Tru9I TTAA 5 cut(s) 132, 393, 413, 573, 932
TscAI CASTG 3 cut(s) 91, 255, 450
TseFI GTSAC 3 cut(s) 211, 435, 857
TseI GCWGC 8 cut(s) 35, 347, 350, 609, 695, 845, 1040, 1043
Tsp45I GTSAC 3 cut(s) 211, 435, 857
TspDTI ATGAA 3 cut(s) 280, 770, 952
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 3 cut(s) 91, 255, 450
Van91I CCANNNNNTGG 1 cut(s) 818
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 3 cut(s) 555, 644, 926
XapI RAATTY 1 cut(s) 363
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 1 cut(s) 55
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XmiI GTMKAC 2 cut(s) 1098, 1131
XspI CTAG 3 cut(s) 368, 851, 1052
ZraI GACGTC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.