Rh3AG229300

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001646
Physical Location & Seq
Forward (+)
23642023 .. 23646873
4851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG229300.1

Sequence Viewer

Length: 1176 bp
ATGGCCACTACTGCAGCTCCTGGTTCACACATGGCAGCAATTAAGCCTTCCATATCTTCTTCCCGAAGAACATACATGTCATGTACTGCCAATTTCGGTGCTAACAGTGAAGTGGCGGTTTCTAAGGGGCTTAGAAGCTCTTCCCACATCTCATCAAGGCAACCATTCTTCCCGAGCATCCAATCAGGCCCTGCGAAGTCTGTAAAGGTTATCACAAGAGCGATGTCAGCAGCAGCTGAGAACACTCCATTGCCTGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGTGTGGCTGATGATAATGGATATGGATGGGCAATAGCAAAACAACTTGCTGCTGCTGGTGCTGAAATTCTTCTTGGTACATGGGTGCCGGCACTTAACATTTTCGAGTCCAGTTTAAGGCGTGGGAAGTTTGATGAATCACGCATATTGCCCGACGGTTCTTTGATGGAAATTACAAAAGTCTATCCATTGGATGCAGTTTTTGACAATCCTGATGATGTTCCTGAAGATATAAAATCAAACAAACGCTACTTGGGGTCCTCAAAGTGGACGGTTAAGGAGGTTGCTGAATCTGTTAAGGAGGATTTTGGCAGCATTGATATCCTAGTCCATTCCCTGGCTAATGGACCAGAGGTTGTCAAACCTCTTATGGAAACATCAAGATATGGATATCTTGCAGCTATCTCTGCATCAAGCTACTCGTTTATTTCTTTACTCCAGCATTTTGCTCCAATAATGAACCCAGGTGGTGCGTCAATCTCTCTGACATACATTGCTTCTGAGAGGATCATTCCCGGATATGGTGGAGGTATGAGCTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGGAGAAAACACAGACTCAGAGTCAACACAATATCTGCAGGCCCATTAAGAAGTCGTGCTGCAAAAGCTATTGGTTTTATCGATATGATGATTGATTACTCATCAGCCAATGCTCCCTTACCGAAAGAGTTGTCTGCTGAGGAGGTGGGGAACGTTGCTGCCTTCCTTGCATCACCATTGGCATCAGCAATCACCGGCAGTGTTGTATACGTCGACAATGGTCTAAATGCAATGGGTGTGGGAGTTGACAGCCCGATTTTTGAAAACCTTGAGCTTCCAAAAGAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

391

Amino Acids

41.33

Weight (kDa)

6.99

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 190 - 371 1.3e-49 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 190 - 318 5.4e-07 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccB7I CCANNNNNTGG 2 cut(s) 254, 634
AccI GTMKAC 2 cut(s) 1092, 1098
AciI CCGC 1 cut(s) 116
AclI AACGTT 1 cut(s) 1038
AclWI GGATC 1 cut(s) 812
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 1 cut(s) 866
AfaI GTAC 2 cut(s) 85, 376
AfiI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
AflIII ACRYGT 3 cut(s) 75, 863, 865
AgsI TTSAA 2 cut(s) 881, 1148
AhdI GACNNNNNGTC 1 cut(s) 905
AjnI CCWGG 4 cut(s) 19, 253, 633, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 9 cut(s) 17, 138, 236, 698, 714, 834, 884, 953, 1159
AluI AGCT 9 cut(s) 17, 138, 236, 698, 714, 834, 884, 953, 1159
Alw21I GWGCWC 1 cut(s) 836
AlwI GGATC 1 cut(s) 812
AlwNI CAGNNNCTG 3 cut(s) 20, 191, 236
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 4 cut(s) 3, 187, 873, 925
ApoI RAATTY 1 cut(s) 363
ArsI GACNNNNNNTTYG 2 cut(s) 1001, 1033
Asp700I GAANNNNTTC 2 cut(s) 139, 366
AspS9I GGNCC 4 cut(s) 188, 555, 644, 926
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 2 cut(s) 1050, 1069
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 2 cut(s) 555, 644
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 836
BbrPI CACGTG 1 cut(s) 866
Bbv12I GWGCWC 1 cut(s) 836
BbvCI CCTCAGC 1 cut(s) 1023
BccI CCATC 2 cut(s) 318, 457
BciT130I CCWGG 4 cut(s) 21, 255, 635, 762
BcnI CCSGG 1 cut(s) 813
BfaI CTAG 3 cut(s) 623, 851, 1174
BfmI CTRYAG 2 cut(s) 12, 921
BlpI GCTNAGC 1 cut(s) 835
Bme1390I CCNGG 5 cut(s) 21, 255, 635, 762, 813
Bme18I GGWCC 2 cut(s) 555, 644
BmeRI GACNNNNNGTC 1 cut(s) 905
BmeT110I CYCGRG 1 cut(s) 172
BmgT120I GGNCC 4 cut(s) 188, 555, 644, 926
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 5 cut(s) 21, 255, 635, 762, 813
BmsI GCATC 5 cut(s) 186, 481, 716, 1064, 1076
BoxI GACNNNNGTC 1 cut(s) 1104
BpmI CTGGAG 1 cut(s) 719
Bpu10I CCTNAGC 1 cut(s) 1023
Bpu1102I GCTNAGC 1 cut(s) 835
BpuEI CTTGAG 1 cut(s) 1175
BpuMI CCSGG 1 cut(s) 813
Bsa29I ATCGAT 1 cut(s) 966
BsaAI YACGTR 1 cut(s) 866
BsaJI CCNNGG 2 cut(s) 633, 760
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
Bse118I RCCGGY 2 cut(s) 385, 1079
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 4 cut(s) 248, 291, 789, 1122
BseBI CCWGG 4 cut(s) 21, 255, 635, 762
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 2 cut(s) 633, 760
BseGI GGATG 3 cut(s) 177, 329, 496
BseLI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
BseMI GCAATG 4 cut(s) 248, 291, 789, 1122
BseMII CTCAG 5 cut(s) 228, 789, 849, 916, 1014
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 1040
BseYI CCCAGC 1 cut(s) 884
BshFI GGCC 4 cut(s) 5, 189, 875, 927
BshNI GGYRCC 1 cut(s) 382
BshVI ATCGAT 1 cut(s) 966
BsiHKAI GWGCWC 1 cut(s) 836
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 3 cut(s) 386, 813, 1080
BslI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 4 cut(s) 5, 189, 875, 927
BsoBI CYCGRG 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 836
Bsp143I GATC 1 cut(s) 804
Bsp1720I GCTNAGC 1 cut(s) 835
BspACI CCGC 1 cut(s) 116
BspANI GGCC 4 cut(s) 5, 189, 875, 927
BspCNI CTCAG 5 cut(s) 229, 790, 848, 915, 1015
BspDI ATCGAT 1 cut(s) 966
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 2 cut(s) 16, 925
BspPI GGATC 1 cut(s) 812
BspQI GCTCTTC 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 4 cut(s) 248, 291, 789, 1122
BsrFI RCCGGY 2 cut(s) 385, 1079
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 2 cut(s) 385, 1079
BssECI CCNNGG 2 cut(s) 633, 760
BssMI GATC 1 cut(s) 804
BssNAI GTATAC 1 cut(s) 1093
Bst1107I GTATAC 1 cut(s) 1093
Bst2UI CCWGG 4 cut(s) 21, 255, 635, 762
Bst4CI ACNGT 3 cut(s) 107, 455, 571
Bst6I CTCTTC 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 387, 873, 925
BstDEI CTNAG 7 cut(s) 123, 131, 237, 798, 835, 902, 1023
BstF5I GGATG 3 cut(s) 177, 329, 496
BstKTI GATC 1 cut(s) 807
BstMBI GATC 1 cut(s) 804
BstNI CCWGG 4 cut(s) 21, 255, 635, 762
BstNSI RCATGY 1 cut(s) 79
BstPAI GACNNNNGTC 1 cut(s) 1104
BstSCI CCNGG 5 cut(s) 19, 253, 633, 760, 811
BstSFI CTRYAG 2 cut(s) 12, 921
BstZ17I GTATAC 1 cut(s) 1093
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 4 cut(s) 5, 189, 875, 927
BsuTUI ATCGAT 1 cut(s) 966
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 3 cut(s) 177, 329, 496
BtsI GCAGTG 1 cut(s) 1090
BtsIMutI CAGTG 2 cut(s) 112, 1090
Cac8I GCNNGC 3 cut(s) 387, 873, 925
CaiI CAGNNNCTG 3 cut(s) 20, 191, 236
Cfr10I RCCGGY 2 cut(s) 385, 1079
Cfr13I GGNCC 4 cut(s) 188, 555, 644, 926
ClaI ATCGAT 1 cut(s) 966
CseI GACGC 1 cut(s) 759
Csp6I GTAC 2 cut(s) 84, 375
CspCI CAANNNNNGTGG 2 cut(s) 1104, 1139
CviAII CATG 4 cut(s) 31, 76, 81, 378
CviQI GTAC 2 cut(s) 84, 375
DdeI CTNAG 7 cut(s) 123, 131, 237, 798, 835, 902, 1023
DpnI GATC 1 cut(s) 806
DpnII GATC 1 cut(s) 804
DriI GACNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 145
Eam1105I GACNNNNNGTC 1 cut(s) 905
EarI CTCTTC 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 834
Eco24I GRGCYC 1 cut(s) 836
Eco32I GATATC 2 cut(s) 619, 689
Eco47I GGWCC 2 cut(s) 555, 644
Eco53kI GAGCTC 1 cut(s) 834
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 1 cut(s) 866
Eco88I CYCGRG 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 834
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoRII CCWGG 4 cut(s) 19, 253, 633, 760
EcoRV GATATC 2 cut(s) 619, 689
EcoT38I GRGCYC 1 cut(s) 836
FaeI CATG 4 cut(s) 34, 79, 84, 381
FatI CATG 4 cut(s) 30, 75, 80, 377
FblI GTMKAC 2 cut(s) 1092, 1098
FokI GGATG 3 cut(s) 164, 336, 503
FriOI GRGCYC 1 cut(s) 836
FspBI CTAG 3 cut(s) 623, 851, 1174
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 4 cut(s) 5, 189, 875, 927
HapII CCGG 3 cut(s) 386, 813, 1080
HgaI GACGC 1 cut(s) 759
Hin1II CATG 4 cut(s) 34, 79, 84, 381
HincII GTYRAC 3 cut(s) 910, 1099, 1132
HindII GTYRAC 3 cut(s) 910, 1099, 1132
HinfI GANTC 5 cut(s) 404, 434, 587, 900, 906
HpaII CCGG 3 cut(s) 386, 813, 1080
HphI GGTGA 2 cut(s) 1050, 1069
Hpy166II GTNNAC 6 cut(s) 26, 567, 910, 1093, 1099, 1132
Hpy188I TCNGA 3 cut(s) 783, 799, 905
Hpy188III TCNNGA 6 cut(s) 63, 172, 509, 521, 678, 851
Hpy8I GTNNAC 6 cut(s) 26, 567, 910, 1093, 1099, 1132
Hpy99I CGWCG 2 cut(s) 455, 1100
HpyAV CCTTC 3 cut(s) 57, 835, 1057
HpyCH4III ACNGT 3 cut(s) 107, 455, 571
HpyCH4IV ACGT 3 cut(s) 865, 1038, 1095
HpyCH4V TGCA 8 cut(s) 14, 494, 695, 707, 923, 947, 1055, 1115
HpyF3I CTNAG 7 cut(s) 123, 131, 237, 798, 835, 902, 1023
HpySE526I ACGT 3 cut(s) 865, 1038, 1095
Hsp92II CATG 4 cut(s) 34, 79, 84, 381
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 804
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 3 cut(s) 22, 751, 1003
LweI GCATC 5 cut(s) 186, 481, 716, 1064, 1076
MaeI CTAG 3 cut(s) 623, 851, 1174
MaeII ACGT 3 cut(s) 865, 1038, 1095
MaeIII GTNAC 1 cut(s) 857
MalI GATC 1 cut(s) 806
MboI GATC 1 cut(s) 804
MboII GAAGA 7 cut(s) 48, 51, 78, 132, 160, 359, 536
MhlI GDGCHC 1 cut(s) 836
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 39, 91, 363, 468
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 413, 894, 915
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MroXI GAANNNNTTC 2 cut(s) 139, 366
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
MslI CAYNNNNRTG 1 cut(s) 866
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 386, 813, 1080
MspR9I CCNGG 5 cut(s) 21, 255, 635, 762, 813
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 4 cut(s) 21, 255, 635, 762
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 813
NdeII GATC 1 cut(s) 804
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 4 cut(s) 34, 79, 84, 381
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 857
NspI RCATGY 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 866
PciI ACATGT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 145
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PdmI GAANNNNTTC 2 cut(s) 139, 366
PfeI GAWTC 2 cut(s) 434, 587
PflMI CCANNNNNTGG 2 cut(s) 254, 634
PfoI TCCNGGA 1 cut(s) 811
PleI GAGTC 3 cut(s) 412, 894, 914
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
PpsI GAGTC 3 cut(s) 412, 894, 914
Ppu21I YACGTR 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 555
PscI ACATGT 1 cut(s) 75
PshAI GACNNNNGTC 1 cut(s) 1104
Psp124BI GAGCTC 1 cut(s) 836
Psp1406I AACGTT 1 cut(s) 1038
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 4 cut(s) 19, 253, 633, 760
PspCI CACGTG 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 4 cut(s) 19, 253, 633, 760
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 4 cut(s) 188, 555, 644, 926
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 2 cut(s) 16, 925
PstNI CAGNNNCTG 3 cut(s) 20, 191, 236
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 85, 376
RsaNI GTAC 2 cut(s) 84, 375
RseI CAYNNNNRTG 1 cut(s) 866
SacI GAGCTC 1 cut(s) 836
SalI GTCGAC 1 cut(s) 1097
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
Sau3AI GATC 1 cut(s) 804
Sau96I GGNCC 4 cut(s) 188, 555, 644, 926
SchI GAGTC 3 cut(s) 413, 894, 915
ScrFI CCNGG 5 cut(s) 21, 255, 635, 762, 813
SduI GDGCHC 1 cut(s) 836
SfaNI GCATC 5 cut(s) 186, 481, 716, 1064, 1076
SfcI CTRYAG 2 cut(s) 12, 921
SinI GGWCC 2 cut(s) 555, 644
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 1154
SmoI CTYRAG 1 cut(s) 1154
Sse9I AATT 4 cut(s) 39, 91, 363, 468
SsiI CCGC 1 cut(s) 116
SspMI CTAG 3 cut(s) 623, 851, 1174
SstI GAGCTC 1 cut(s) 836
StyD4I CCNGG 5 cut(s) 19, 253, 633, 760, 811
TaaI ACNGT 3 cut(s) 107, 455, 571
TaiI ACGT 3 cut(s) 868, 1041, 1098
TaqI TCGA 3 cut(s) 402, 966, 1098
TasI AATT 4 cut(s) 39, 91, 363, 468
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 2 cut(s) 434, 587
Tru1I TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
Tru9I TTAA 6 cut(s) 42, 393, 413, 573, 594, 932
TscAI CASTG 2 cut(s) 112, 1090
TseFI GTSAC 1 cut(s) 857
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 3 cut(s) 280, 447, 770
TspRI CASTG 2 cut(s) 112, 1090
Van91I CCANNNNNTGG 2 cut(s) 254, 634
VpaK11BI GGWCC 2 cut(s) 555, 644
XapI RAATTY 1 cut(s) 363
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 1092, 1098
XmnI GAANNNNTTC 2 cut(s) 139, 366
XspI CTAG 3 cut(s) 623, 851, 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.