Rw0G019220

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00864
OG0001646
Physical Location & Seq
Forward (+)
55085 .. 58925
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019220.1

Sequence Viewer

Length: 1140 bp
ATGGCCACTACTGCAGCTCCTGGTTCACACATGGCAGCAATTAAGCCTTCCATATCTTCTTCCCGAAGAGCATACATGTCATGTAATGGCAATTTCGGTGCTAACAGTGAAGTGGCAGTTTCTAAGGGGCTTAGAAGCTCTTCCCACATCTCATCAAGGCAACCATTCTTCCCGAGCATCCAATCAGGCCCTGCGAAGTCTGTAAAGGTTATCACAAGAGCGATGTCAGCAGCAGCTGAGAACACTCCATTGCCTGGATTGCCTATCGATTTGAGAGAGAGCATTCATTGCTGGGTGGCTGATGATAATGGATATGGATGGGCAATAGCAAAACAACTTGCTGCTGCGCTGGTGCTGAAATTCTTCTTGGCACTTAACAGTTTCGAGTCCAGTTTAAGGCGTGGGAAATTGCCCGACGGTTCTTTGATGGAAATTACAAAAGTCTATCCATTGGATGCAGTTTTTGACAATCCTGATGATGTTCCTGAAGATATAAAATCAAACAAACGCTACTTGGGGTCCTCAAAGTGGACGGTTAAGGAGGTTGCTGAATCTGTTAAGGAGGATTTTGGCAGCATTGATATCCTAGTCCATTCCCTGGCTAATGGACCAGAGGTTGTCAAACCTCTTATGGAAACATCAAGATATGGATATCTTGCAGCTATCTCTGCATCAAGCTACTCGTTTATTTCTTTACTCCAGCATTTTGCTCCAATAATGAACCCAGGTGGTGCGTCAATCTCTCTGACATACATTGCTTCCGAGAGGATCATTCCCGGATATGGTGGAGGTATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGGAGAAAACACAGACTCAGAGTCAACACAATATCTGCAGGCCCATTAAGAAGTCGTGCTGCAAAAGCTATTGGTTTTATCGATATGATGATTGATTACTCATCAGCCAATGCTCCCTTACCGAAAGAGTTGTCTGCTGAGGAGGTGGGGAACGTTGCTGCCTTCCTTGCATCACCATTGGCATCAGCAATCACCGGCAGTGTTGTATACGTCGACAATGGTCTAAATGCAATGGGTGTGGGAGTTGACAGCCCAATTTTTGAAAACCTTGAGCTTCCAAAAGAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

379

Amino Acids

40.21

Weight (kDa)

7.02

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 178 - 359 1.2e-49 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 178 - 306 5e-07 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 254, 598
AccI GTMKAC 2 cut(s) 1056, 1062
AclI AACGTT 1 cut(s) 1002
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 1 cut(s) 507
AcvI CACGTG 1 cut(s) 830
AfiI CCNNNNNNNGG 5 cut(s) 254, 396, 528, 598, 782
AflIII ACRYGT 3 cut(s) 75, 827, 829
AgsI TTSAA 2 cut(s) 845, 1112
AhdI GACNNNNNGTC 1 cut(s) 869
AjnI CCWGG 4 cut(s) 19, 253, 597, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AleI CACNNNNGTG 1 cut(s) 830
AluBI AGCT 8 cut(s) 17, 138, 236, 662, 678, 848, 917, 1123
AluI AGCT 8 cut(s) 17, 138, 236, 662, 678, 848, 917, 1123
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 3 cut(s) 20, 191, 236
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 4 cut(s) 3, 187, 837, 889
ApoI RAATTY 1 cut(s) 359
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
Asp700I GAANNNNTTC 2 cut(s) 139, 362
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 4 cut(s) 188, 519, 608, 890
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 2 cut(s) 1014, 1033
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 2 cut(s) 519, 608
BalI TGGCCA 1 cut(s) 5
BbrPI CACGTG 1 cut(s) 830
BbvCI CCTCAGC 1 cut(s) 987
BccI CCATC 2 cut(s) 312, 421
BciT130I CCWGG 4 cut(s) 21, 255, 599, 726
BcnI CCSGG 1 cut(s) 777
BfaI CTAG 3 cut(s) 587, 815, 1138
BfmI CTRYAG 2 cut(s) 12, 885
Bme1390I CCNGG 5 cut(s) 21, 255, 599, 726, 777
Bme18I GGWCC 2 cut(s) 519, 608
BmeRI GACNNNNNGTC 1 cut(s) 869
BmeT110I CYCGRG 1 cut(s) 172
BmgT120I GGNCC 4 cut(s) 188, 519, 608, 890
BmiI GGNNCC 1 cut(s) 520
BmrFI CCNGG 5 cut(s) 21, 255, 599, 726, 777
BmsI GCATC 5 cut(s) 186, 445, 680, 1028, 1040
BoxI GACNNNNGTC 1 cut(s) 1068
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 1139
BpuMI CCSGG 1 cut(s) 777
Bsa29I ATCGAT 2 cut(s) 267, 930
BsaAI YACGTR 1 cut(s) 830
BsaJI CCNNGG 2 cut(s) 597, 724
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 396, 528, 598, 782
Bse118I RCCGGY 1 cut(s) 1043
Bse1I ACTGG 1 cut(s) 390
Bse3DI GCAATG 4 cut(s) 248, 286, 753, 1086
BseBI CCWGG 4 cut(s) 21, 255, 599, 726
BseCI ATCGAT 2 cut(s) 267, 930
BseDI CCNNGG 2 cut(s) 597, 724
BseGI GGATG 3 cut(s) 177, 323, 460
BseLI CCNNNNNNNGG 5 cut(s) 254, 396, 528, 598, 782
BseMI GCAATG 4 cut(s) 248, 286, 753, 1086
BseMII CTCAG 3 cut(s) 228, 880, 978
BseNI ACTGG 1 cut(s) 390
BseRI GAGGAG 1 cut(s) 1004
BseYI CCCAGC 2 cut(s) 291, 848
BshFI GGCC 4 cut(s) 5, 189, 839, 891
BshVI ATCGAT 2 cut(s) 267, 930
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 2 cut(s) 777, 1044
BslI CCNNNNNNNGG 5 cut(s) 254, 396, 528, 598, 782
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 4 cut(s) 5, 189, 839, 891
BsoBI CYCGRG 1 cut(s) 172
Bsp143I GATC 1 cut(s) 768
BspANI GGCC 4 cut(s) 5, 189, 839, 891
BspCNI CTCAG 3 cut(s) 229, 879, 979
BspDI ATCGAT 2 cut(s) 267, 930
BspLI GGNNCC 1 cut(s) 520
BspMAI CTGCAG 2 cut(s) 16, 889
BspPI GGATC 1 cut(s) 776
BspQI GCTCTTC 2 cut(s) 61, 145
BsrDI GCAATG 4 cut(s) 248, 286, 753, 1086
BsrFI RCCGGY 1 cut(s) 1043
BsrI ACTGG 1 cut(s) 390
BssAI RCCGGY 1 cut(s) 1043
BssECI CCNNGG 2 cut(s) 597, 724
BssMI GATC 1 cut(s) 768
BssNAI GTATAC 1 cut(s) 1057
Bst1107I GTATAC 1 cut(s) 1057
Bst2UI CCWGG 4 cut(s) 21, 255, 599, 726
Bst4CI ACNGT 4 cut(s) 107, 380, 419, 535
Bst6I CTCTTC 2 cut(s) 61, 145
BstAPI GCANNNNNTGC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 830
BstC8I GCNNGC 2 cut(s) 837, 889
BstDEI CTNAG 5 cut(s) 123, 131, 237, 866, 987
BstF5I GGATG 3 cut(s) 177, 323, 460
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 1 cut(s) 771
BstMBI GATC 1 cut(s) 768
BstMWI GCNNNNNNNGC 9 cut(s) 11, 227, 259, 288, 668, 809, 845, 914, 1016
BstNI CCWGG 4 cut(s) 21, 255, 599, 726
BstNSI RCATGY 1 cut(s) 79
BstPAI GACNNNNGTC 1 cut(s) 1068
BstSCI CCNGG 5 cut(s) 19, 253, 597, 724, 775
BstSFI CTRYAG 2 cut(s) 12, 885
BstZ17I GTATAC 1 cut(s) 1057
Bsu15I ATCGAT 2 cut(s) 267, 930
BsuRI GGCC 4 cut(s) 5, 189, 839, 891
BsuTUI ATCGAT 2 cut(s) 267, 930
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 3 cut(s) 177, 323, 460
BtsI GCAGTG 1 cut(s) 1054
BtsIMutI CAGTG 2 cut(s) 112, 1054
Cac8I GCNNGC 2 cut(s) 837, 889
CaiI CAGNNNCTG 3 cut(s) 20, 191, 236
CfoI GCGC 1 cut(s) 349
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 4 cut(s) 188, 519, 608, 890
ClaI ATCGAT 2 cut(s) 267, 930
CseI GACGC 1 cut(s) 723
CspCI CAANNNNNGTGG 2 cut(s) 1068, 1103
CviAII CATG 3 cut(s) 31, 76, 81
DdeI CTNAG 5 cut(s) 123, 131, 237, 866, 987
DpnI GATC 1 cut(s) 770
DpnII GATC 1 cut(s) 768
DriI GACNNNNNGTC 1 cut(s) 869
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 61, 145
Eam1105I GACNNNNNGTC 1 cut(s) 869
EarI CTCTTC 2 cut(s) 61, 145
Eco32I GATATC 2 cut(s) 583, 653
Eco47I GGWCC 2 cut(s) 519, 608
Eco57I CTGAAG 1 cut(s) 507
Eco72I CACGTG 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 172
EcoO109I RGGNCCY 2 cut(s) 188, 519
EcoRII CCWGG 4 cut(s) 19, 253, 597, 724
EcoRV GATATC 2 cut(s) 583, 653
FaeI CATG 3 cut(s) 34, 79, 84
FatI CATG 3 cut(s) 30, 75, 80
FblI GTMKAC 2 cut(s) 1056, 1062
FokI GGATG 3 cut(s) 164, 330, 467
FspBI CTAG 3 cut(s) 587, 815, 1138
GlaI GCGC 1 cut(s) 348
GsaI CCCAGC 2 cut(s) 295, 852
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 4 cut(s) 5, 189, 839, 891
HapII CCGG 2 cut(s) 777, 1044
HgaI GACGC 1 cut(s) 723
HhaI GCGC 1 cut(s) 349
Hin1II CATG 3 cut(s) 34, 79, 84
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 3 cut(s) 874, 1063, 1096
HindII GTYRAC 3 cut(s) 874, 1063, 1096
HinfI GANTC 4 cut(s) 386, 551, 864, 870
HpaII CCGG 2 cut(s) 777, 1044
HphI GGTGA 2 cut(s) 1014, 1033
Hpy166II GTNNAC 6 cut(s) 26, 531, 874, 1057, 1063, 1096
Hpy188I TCNGA 3 cut(s) 747, 763, 869
Hpy188III TCNNGA 6 cut(s) 63, 172, 473, 485, 642, 815
Hpy8I GTNNAC 6 cut(s) 26, 531, 874, 1057, 1063, 1096
Hpy99I CGWCG 2 cut(s) 419, 1064
HpyAV CCTTC 3 cut(s) 57, 799, 1021
HpyCH4III ACNGT 4 cut(s) 107, 380, 419, 535
HpyCH4IV ACGT 3 cut(s) 829, 1002, 1059
HpyCH4V TGCA 8 cut(s) 14, 458, 659, 671, 887, 911, 1019, 1079
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 227, 259, 288, 668, 809, 845, 914, 1016
HpyF3I CTNAG 5 cut(s) 123, 131, 237, 866, 987
HpySE526I ACGT 3 cut(s) 829, 1002, 1059
Hsp92II CATG 3 cut(s) 34, 79, 84
HspAI GCGC 1 cut(s) 347
Kzo9I GATC 1 cut(s) 768
LguI GCTCTTC 2 cut(s) 61, 145
LmnI GCTCC 3 cut(s) 22, 715, 967
LweI GCATC 5 cut(s) 186, 445, 680, 1028, 1040
MaeI CTAG 3 cut(s) 587, 815, 1138
MaeII ACGT 3 cut(s) 829, 1002, 1059
MaeIII GTNAC 1 cut(s) 821
MalI GATC 1 cut(s) 770
MboI GATC 1 cut(s) 768
MboII GAAGA 7 cut(s) 48, 51, 78, 132, 160, 355, 500
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 39, 91, 359, 407, 432, 1104
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 395, 858, 879
MnlI CCTC 9 cut(s) 532, 535, 556, 607, 636, 759, 782, 982, 985
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 139, 362
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 42, 375, 395, 537, 558, 896
MslI CAYNNNNRTG 1 cut(s) 830
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 2 cut(s) 777, 1044
MspR9I CCNGG 5 cut(s) 21, 255, 599, 726, 777
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 4 cut(s) 21, 255, 599, 726
MwoI GCNNNNNNNGC 9 cut(s) 11, 227, 259, 288, 668, 809, 845, 914, 1016
NciI CCSGG 1 cut(s) 777
NdeII GATC 1 cut(s) 768
NlaIII CATG 3 cut(s) 34, 79, 84
NlaIV GGNNCC 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 821
NspI RCATGY 1 cut(s) 79
OliI CACNNNNGTG 1 cut(s) 830
PciI ACATGT 1 cut(s) 75
PciSI GCTCTTC 2 cut(s) 61, 145
PctI GAATGC 1 cut(s) 282
PdmI GAANNNNTTC 2 cut(s) 139, 362
PfeI GAWTC 1 cut(s) 551
PflMI CCANNNNNTGG 2 cut(s) 254, 598
PfoI TCCNGGA 1 cut(s) 775
PleI GAGTC 3 cut(s) 394, 858, 878
PmaCI CACGTG 1 cut(s) 830
PmlI CACGTG 1 cut(s) 830
PpsI GAGTC 3 cut(s) 394, 858, 878
Ppu21I YACGTR 1 cut(s) 830
PpuMI RGGWCCY 1 cut(s) 519
PscI ACATGT 1 cut(s) 75
PshAI GACNNNNGTC 1 cut(s) 1068
Psp1406I AACGTT 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 519
Psp6I CCWGG 4 cut(s) 19, 253, 597, 724
PspCI CACGTG 1 cut(s) 830
PspFI CCCAGC 2 cut(s) 291, 848
PspGI CCWGG 4 cut(s) 19, 253, 597, 724
PspN4I GGNNCC 1 cut(s) 520
PspPI GGNCC 4 cut(s) 188, 519, 608, 890
PspPPI RGGWCCY 1 cut(s) 519
PstI CTGCAG 2 cut(s) 16, 889
PstNI CAGNNNCTG 3 cut(s) 20, 191, 236
PvuII CAGCTG 1 cut(s) 236
RseI CAYNNNNRTG 1 cut(s) 830
SalI GTCGAC 1 cut(s) 1061
SapI GCTCTTC 2 cut(s) 61, 145
SaqAI TTAA 6 cut(s) 42, 375, 395, 537, 558, 896
Sau3AI GATC 1 cut(s) 768
Sau96I GGNCC 4 cut(s) 188, 519, 608, 890
SchI GAGTC 3 cut(s) 395, 858, 879
ScrFI CCNGG 5 cut(s) 21, 255, 599, 726, 777
SfaNI GCATC 5 cut(s) 186, 445, 680, 1028, 1040
SfcI CTRYAG 2 cut(s) 12, 885
SinI GGWCC 2 cut(s) 519, 608
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 1 cut(s) 1118
SmoI CTYRAG 1 cut(s) 1118
Sse9I AATT 6 cut(s) 39, 91, 359, 407, 432, 1104
SspMI CTAG 3 cut(s) 587, 815, 1138
StyD4I CCNGG 5 cut(s) 19, 253, 597, 724, 775
TaaI ACNGT 4 cut(s) 107, 380, 419, 535
TaiI ACGT 3 cut(s) 832, 1005, 1062
TaqI TCGA 4 cut(s) 267, 384, 930, 1062
TasI AATT 6 cut(s) 39, 91, 359, 407, 432, 1104
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 6 cut(s) 42, 375, 395, 537, 558, 896
Tru9I TTAA 6 cut(s) 42, 375, 395, 537, 558, 896
TscAI CASTG 2 cut(s) 112, 1054
TseFI GTSAC 1 cut(s) 821
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 2 cut(s) 275, 734
TspRI CASTG 2 cut(s) 112, 1054
Van91I CCANNNNNTGG 2 cut(s) 254, 598
VpaK11BI GGWCC 2 cut(s) 519, 608
XapI RAATTY 1 cut(s) 359
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 1056, 1062
XmnI GAANNNNTTC 2 cut(s) 139, 362
XspI CTAG 3 cut(s) 587, 815, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.