Rh3CG259400

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001646
Physical Location & Seq
Forward (+)
25331503 .. 25336500
4998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG259400.1

Sequence Viewer

Length: 981 bp
ATGGCCACTACTGCAGCTCCTGGTTCACACATGGCAGCAATTAAGCCTTCCATATCTTCTTCCGGAAGAACATTCATGTCATGTACTGCCAATTTCGGTGCTAACAGTGAAGTGGCAGTTTCTAAGGGGCTTAGAAGCTCTTCCCACATCTCATCAAGGCAACCATTCTTCCGGAGCATCCAATCAGGCTCTGCGAAGTCTGTAAAGGTTATCACAAGAGCGATGTCAGCAGCAGCTGAGAACTCTCCATTGCCTGGATTACCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGTGTGGCTGATGATAATGGATATGGATGGGCAATAGCAAAACAACTTGCTGCTGCTGGTGCTGAAATTCTTCTTGGTACATGGGTGCCGGCACTTAACATTTTCGAGTCCAGTTTAAGGCGTGGGAAGTTTGATGAATCACGCATATTGCCCGACGGTTCTTTGATGGAAATTACAAAAGTCTATCCATTGGATGCAGTTTTTGACAATCCTGATGATGTTCCTGAAGATATAAAATCAAACAAACGCTACTTGGGGTCCTCAAAGTGGACGGTTAAGGAGGTTGCTGAATCTGTTAAGGAGGATTTTGGCAGCATTGATATCCTAGTCCATTCCCTGGCTAATGGACCAGAGGTTGTCAAACCTCTTATGGAAACATCAAGATATGGATATCTTGCAGCTATCTCTGCATCAAGCTACTCGTTTATTTCTTTACTCCAGCATTTTGCCCCAATAATGAACCCAGGTGGTGCGTCAATCTCTCTGACATACATTGCTTCTGAGAGGATCATTCCCGGATATGGTGGAGGTATGAGTTCAGCGAAGGCTGCTCTAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGGAGAAAACACAGACTCAGAGTCAACACAATATCTGCAGGTACTTCGATAAACTATTTTTACAGAATCAGTCTGACACTCTTCTTGCAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

326

Amino Acids

34.84

Weight (kDa)

9.3

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 190 - 310 5e-29 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 190 - 309 4.9e-06 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 914
AccB1I GGYRCC 1 cut(s) 382
AccB7I CCANNNNNTGG 2 cut(s) 254, 634
AccIII TCCGGA 2 cut(s) 62, 171
AclWI GGATC 1 cut(s) 812
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 1 cut(s) 866
AfaI GTAC 3 cut(s) 85, 376, 928
AfiI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
AflIII ACRYGT 2 cut(s) 863, 865
AgsI TTSAA 1 cut(s) 881
AhdI GACNNNNNGTC 1 cut(s) 905
AjnI CCWGG 4 cut(s) 19, 253, 633, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 1 cut(s) 866
AluBI AGCT 6 cut(s) 17, 138, 236, 698, 714, 884
AluI AGCT 6 cut(s) 17, 138, 236, 698, 714, 884
AlwI GGATC 1 cut(s) 812
AlwNI CAGNNNCTG 3 cut(s) 20, 191, 236
Aor13HI TCCGGA 2 cut(s) 62, 171
AoxI GGCC 2 cut(s) 3, 873
ApeKI GCWGC 9 cut(s) 14, 35, 230, 233, 347, 350, 609, 695, 845
ApoI RAATTY 1 cut(s) 363
Asp700I GAANNNNTTC 2 cut(s) 139, 366
AspS9I GGNCC 2 cut(s) 555, 644
AsuC2I CCSGG 1 cut(s) 813
AvaII GGWCC 2 cut(s) 555, 644
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BbrPI CACGTG 1 cut(s) 866
BbvI GCAGC 9 cut(s) 26, 47, 242, 245, 334, 337, 621, 707, 832
BccI CCATC 2 cut(s) 318, 457
BcgI CGANNNNNNTGC 2 cut(s) 912, 946
BciT130I CCWGG 4 cut(s) 21, 255, 635, 762
BcnI CCSGG 1 cut(s) 813
BfaI CTAG 2 cut(s) 623, 851
BfmI CTRYAG 2 cut(s) 12, 921
BfuAI ACCTGC 1 cut(s) 914
BisI GCNGC 9 cut(s) 15, 36, 231, 234, 348, 351, 610, 696, 846
BlsI GCNGC 9 cut(s) 16, 37, 232, 235, 349, 352, 611, 697, 847
Bme1390I CCNGG 5 cut(s) 21, 255, 635, 762, 813
Bme18I GGWCC 2 cut(s) 555, 644
BmeRI GACNNNNNGTC 1 cut(s) 905
BmgT120I GGNCC 2 cut(s) 555, 644
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 5 cut(s) 21, 255, 635, 762, 813
BmsI GCATC 3 cut(s) 186, 481, 716
BpmI CTGGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 866
BsaJI CCNNGG 2 cut(s) 633, 760
BsaWI WCCGGW 2 cut(s) 62, 171
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 3 cut(s) 248, 291, 789
BseAI TCCGGA 2 cut(s) 62, 171
BseBI CCWGG 4 cut(s) 21, 255, 635, 762
BseDI CCNNGG 2 cut(s) 633, 760
BseGI GGATG 3 cut(s) 177, 329, 496
BseLI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
BseMI GCAATG 3 cut(s) 248, 291, 789
BseMII CTCAG 3 cut(s) 228, 789, 916
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 9 cut(s) 26, 47, 242, 245, 334, 337, 621, 707, 832
BseYI CCCAGC 1 cut(s) 884
BshFI GGCC 2 cut(s) 5, 875
BshNI GGYRCC 1 cut(s) 382
BsiSI CCGG 4 cut(s) 63, 172, 386, 813
BslI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 818
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 2 cut(s) 5, 875
Bsp13I TCCGGA 2 cut(s) 62, 171
Bsp143I GATC 1 cut(s) 804
BspANI GGCC 2 cut(s) 5, 875
BspCNI CTCAG 3 cut(s) 229, 790, 915
BspEI TCCGGA 2 cut(s) 62, 171
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 2 cut(s) 16, 925
BspMI ACCTGC 1 cut(s) 914
BspPI GGATC 1 cut(s) 812
BspQI GCTCTTC 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 3 cut(s) 248, 291, 789
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 633, 760
BssMI GATC 1 cut(s) 804
Bst2UI CCWGG 4 cut(s) 21, 255, 635, 762
Bst4CI ACNGT 3 cut(s) 107, 455, 571
Bst6I CTCTTC 2 cut(s) 145, 971
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 2 cut(s) 387, 873
BstDEI CTNAG 5 cut(s) 123, 131, 237, 798, 902
BstF5I GGATG 3 cut(s) 177, 329, 496
BstKTI GATC 1 cut(s) 807
BstMBI GATC 1 cut(s) 804
BstMWI GCNNNNNNNGC 8 cut(s) 11, 227, 293, 302, 356, 704, 845, 881
BstNI CCWGG 4 cut(s) 21, 255, 635, 762
BstSCI CCNGG 5 cut(s) 19, 253, 633, 760, 811
BstSFI CTRYAG 2 cut(s) 12, 921
BstV1I GCAGC 9 cut(s) 26, 47, 242, 245, 334, 337, 621, 707, 832
BsuRI GGCC 2 cut(s) 5, 875
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 3 cut(s) 177, 329, 496
BtsIMutI CAGTG 1 cut(s) 112
BveI ACCTGC 1 cut(s) 914
Cac8I GCNNGC 2 cut(s) 387, 873
CaiI CAGNNNCTG 3 cut(s) 20, 191, 236
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 555, 644
CseI GACGC 1 cut(s) 759
Csp6I GTAC 3 cut(s) 84, 375, 927
CviAII CATG 5 cut(s) 31, 76, 81, 378, 978
CviQI GTAC 3 cut(s) 84, 375, 927
DdeI CTNAG 5 cut(s) 123, 131, 237, 798, 902
DpnI GATC 1 cut(s) 806
DpnII GATC 1 cut(s) 804
DriI GACNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 145, 971
Eam1105I GACNNNNNGTC 1 cut(s) 905
EarI CTCTTC 2 cut(s) 145, 971
Eco32I GATATC 2 cut(s) 619, 689
Eco47I GGWCC 2 cut(s) 555, 644
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 1 cut(s) 866
EcoO109I RGGNCCY 1 cut(s) 555
EcoRII CCWGG 4 cut(s) 19, 253, 633, 760
EcoRV GATATC 2 cut(s) 619, 689
FaeI CATG 5 cut(s) 34, 79, 84, 381, 981
FatI CATG 5 cut(s) 30, 75, 80, 377, 977
Fnu4HI GCNGC 9 cut(s) 15, 36, 231, 234, 348, 351, 610, 696, 846
FokI GGATG 3 cut(s) 164, 336, 503
Fsp4HI GCNGC 9 cut(s) 15, 36, 231, 234, 348, 351, 610, 696, 846
FspBI CTAG 2 cut(s) 623, 851
GluI GCNGC 9 cut(s) 15, 36, 231, 234, 348, 351, 610, 696, 846
GsaI CCCAGC 1 cut(s) 888
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 2 cut(s) 5, 875
HapII CCGG 4 cut(s) 63, 172, 386, 813
HgaI GACGC 1 cut(s) 759
Hin1II CATG 5 cut(s) 34, 79, 84, 381, 981
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 6 cut(s) 404, 434, 587, 900, 906, 951
HpaII CCGG 4 cut(s) 63, 172, 386, 813
Hpy166II GTNNAC 3 cut(s) 26, 567, 910
Hpy188I TCNGA 4 cut(s) 783, 799, 905, 960
Hpy188III TCNNGA 6 cut(s) 63, 172, 509, 521, 678, 851
Hpy8I GTNNAC 3 cut(s) 26, 567, 910
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 2 cut(s) 57, 835
HpyCH4III ACNGT 3 cut(s) 107, 455, 571
HpyCH4IV ACGT 1 cut(s) 865
HpyCH4V TGCA 6 cut(s) 14, 494, 695, 707, 923, 973
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 227, 293, 302, 356, 704, 845, 881
HpyF3I CTNAG 5 cut(s) 123, 131, 237, 798, 902
HpySE526I ACGT 1 cut(s) 865
Hsp92II CATG 5 cut(s) 34, 79, 84, 381, 981
Kpn2I TCCGGA 2 cut(s) 62, 171
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 804
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 2 cut(s) 22, 174
Lsp1109I GCAGC 9 cut(s) 26, 47, 242, 245, 334, 337, 621, 707, 832
LweI GCATC 3 cut(s) 186, 481, 716
MaeI CTAG 2 cut(s) 623, 851
MaeII ACGT 1 cut(s) 865
MaeIII GTNAC 1 cut(s) 857
MalI GATC 1 cut(s) 806
MboI GATC 1 cut(s) 804
MboII GAAGA 8 cut(s) 48, 51, 78, 132, 160, 359, 536, 958
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 39, 91, 363, 468
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 413, 894, 915
MnlI CCTC 8 cut(s) 269, 568, 571, 592, 643, 672, 795, 818
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 62, 171
MroNI GCCGGC 1 cut(s) 385
MroXI GAANNNNTTC 2 cut(s) 139, 366
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 42, 393, 413, 573, 594
MslI CAYNNNNRTG 1 cut(s) 866
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 4 cut(s) 63, 172, 386, 813
MspR9I CCNGG 5 cut(s) 21, 255, 635, 762, 813
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 4 cut(s) 21, 255, 635, 762
MwoI GCNNNNNNNGC 8 cut(s) 11, 227, 293, 302, 356, 704, 845, 881
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 813
NdeII GATC 1 cut(s) 804
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 34, 79, 84, 381, 981
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 857
OliI CACNNNNGTG 1 cut(s) 866
PciSI GCTCTTC 1 cut(s) 145
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PdmI GAANNNNTTC 2 cut(s) 139, 366
PfeI GAWTC 3 cut(s) 434, 587, 951
PflMI CCANNNNNTGG 2 cut(s) 254, 634
PfoI TCCNGGA 1 cut(s) 811
PkrI GCNGC 9 cut(s) 16, 37, 232, 235, 349, 352, 611, 697, 847
PleI GAGTC 3 cut(s) 412, 894, 914
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
PpsI GAGTC 3 cut(s) 412, 894, 914
Ppu21I YACGTR 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 4 cut(s) 19, 253, 633, 760
PspCI CACGTG 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 884
PspGI CCWGG 4 cut(s) 19, 253, 633, 760
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 2 cut(s) 555, 644
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 2 cut(s) 16, 925
PstNI CAGNNNCTG 3 cut(s) 20, 191, 236
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 3 cut(s) 85, 376, 928
RsaNI GTAC 3 cut(s) 84, 375, 927
RseI CAYNNNNRTG 1 cut(s) 866
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 5 cut(s) 42, 393, 413, 573, 594
SatI GCNGC 9 cut(s) 15, 36, 231, 234, 348, 351, 610, 696, 846
Sau3AI GATC 1 cut(s) 804
Sau96I GGNCC 2 cut(s) 555, 644
SchI GAGTC 3 cut(s) 413, 894, 915
ScrFI CCNGG 5 cut(s) 21, 255, 635, 762, 813
SfaNI GCATC 3 cut(s) 186, 481, 716
SfcI CTRYAG 2 cut(s) 12, 921
SinI GGWCC 2 cut(s) 555, 644
SmiMI CAYNNNNRTG 1 cut(s) 866
Sse9I AATT 4 cut(s) 39, 91, 363, 468
SspMI CTAG 2 cut(s) 623, 851
StyD4I CCNGG 5 cut(s) 19, 253, 633, 760, 811
TaaI ACNGT 3 cut(s) 107, 455, 571
TaiI ACGT 1 cut(s) 868
TaqI TCGA 2 cut(s) 402, 932
TasI AATT 4 cut(s) 39, 91, 363, 468
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 3 cut(s) 434, 587, 951
Tru1I TTAA 5 cut(s) 42, 393, 413, 573, 594
Tru9I TTAA 5 cut(s) 42, 393, 413, 573, 594
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 9 cut(s) 14, 35, 230, 233, 347, 350, 609, 695, 845
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 4 cut(s) 64, 280, 447, 770
TspRI CASTG 1 cut(s) 112
Van91I CCANNNNNTGG 2 cut(s) 254, 634
VpaK11BI GGWCC 2 cut(s) 555, 644
XapI RAATTY 1 cut(s) 363
XbaI TCTAGA 1 cut(s) 850
XmnI GAANNNNTTC 2 cut(s) 139, 366
XspI CTAG 2 cut(s) 623, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.