MD14G1058600.v1.1

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001646
Physical Location & Seq
Forward (+)
6064143 .. 6067153
3011 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1058600.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGCCATGACTGCAGCTCCCGGATCACACATGGCAGCGGTTAAGCCTTGCATATCGTCCTCTCAAAGAACTTATATGTCATGCACAGCAAAGTTCGGTACAAACAGCGAGGTGGGAGTTTGGAGAAGGCTTAAGAGTTCTTCGCACATCTCAGCAACACAGCCATTCTCACAGAGCTTGAAATCAGGGCCTGCGAAATCCGTGAAGGTTGTCACAAGAGCAATGTCAGGAGCAGCTGAGAACAGTCCACTACCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGTGTGGCTGATGATCATGGATATGGATGGGCAATATCAAAAGCTCTTGCTGCTGCTGGGGCTGAAATTCTAGTTGGTACATGGGTGCCGGCACTGAACATTTTTGAGTCCAGTTTAAGGCGTGGGAAGTTTGACGAGACACGCAAACTGCCAGATGGCTCTTTGATGGAAATTACCAAGGTTTACCCATTAGATGCAGTTTTTGACAGTCCCGAGGATGTTCCTGAAGATATAAAAACCAACAAACGCTACTTGGGGTCCTCAAATTGGACTGTTAAGGAGGTTGTGGAATCTGTGAAGCAGGATTTTGGCAGCATTGATATCCTTGTCCACTCTCTTGCCAATGGGCCAGAGGTTACCAAACCTCTTTTGGAGACGTCAAGATATGGATATCTTGCAGCTATCTCTGCATCAAGTTACTCATATGTTTCTTTACTCAAGCATTTTGCTCCAATAATGAACCCAGGCACTACTCTAACTTTAGTACTTTCTTTCTCCTCCTCCTCCTTCTCTCCTCCGCTCTTCAATTTTCAAAATCCCGTGATGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

283

Amino Acids

30.35

Weight (kDa)

9.11

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 190 - 266 4e-12 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 677
AccB1I GGYRCC 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 817
AciI CCGC 2 cut(s) 38, 815
AclWI GGATC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 3 cut(s) 100, 376, 783
AfiI CCNNNNNNNGG 3 cut(s) 254, 414, 564
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 3 cut(s) 181, 823, 830
AjnI CCWGG 2 cut(s) 253, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AluBI AGCT 5 cut(s) 17, 177, 236, 341, 698
AluI AGCT 5 cut(s) 17, 177, 236, 341, 698
Alw26I GTCTC 2 cut(s) 428, 665
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 3 cut(s) 3, 188, 644
ApeKI GCWGC 7 cut(s) 14, 35, 233, 347, 350, 609, 695
ApoI RAATTY 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 188, 555, 644
AsuC2I CCSGG 1 cut(s) 21
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 555
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 382
BbvI GCAGC 7 cut(s) 26, 47, 245, 334, 337, 621, 707
BccI CCATC 3 cut(s) 318, 446, 457
BciT130I CCWGG 2 cut(s) 255, 762
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 2 cut(s) 428, 665
BfaI CTAG 2 cut(s) 368, 847
BfmI CTRYAG 1 cut(s) 12
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 7 cut(s) 15, 36, 234, 348, 351, 610, 696
BlsI GCNGC 7 cut(s) 16, 37, 235, 349, 352, 611, 697
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 3 cut(s) 21, 255, 762
Bme18I GGWCC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 188, 555, 644
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 3 cut(s) 21, 255, 762
BmsI GCATC 2 cut(s) 481, 716
BpuEI CTTGAG 1 cut(s) 719
BpuMI CCSGG 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 674
BsaJI CCNNGG 3 cut(s) 474, 510, 760
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 414, 564
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 2 cut(s) 228, 291
BseBI CCWGG 2 cut(s) 255, 762
BseDI CCNNGG 3 cut(s) 474, 510, 760
BseGI GGATG 2 cut(s) 329, 520
BseLI CCNNNNNNNGG 3 cut(s) 254, 414, 564
BseMI GCAATG 2 cut(s) 228, 291
BseMII CTCAG 2 cut(s) 165, 228
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 4 cut(s) 784, 787, 790, 801
BseXI GCAGC 7 cut(s) 26, 47, 245, 334, 337, 621, 707
BseYI CCCAGC 1 cut(s) 353
BshFI GGCC 3 cut(s) 5, 190, 646
BshNI GGYRCC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 21, 386
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 3 cut(s) 254, 414, 564
BsmAI GTCTC 2 cut(s) 428, 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 3 cut(s) 5, 190, 646
BsoBI CYCGRG 1 cut(s) 509
Bsp143I GATC 2 cut(s) 23, 310
BspACI CCGC 2 cut(s) 38, 815
BspANI GGCC 3 cut(s) 5, 190, 646
BspCNI CTCAG 2 cut(s) 164, 229
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 31
BspQI GCTCTTC 1 cut(s) 824
BspT107I GGYRCC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 817
BsrDI GCAATG 2 cut(s) 228, 291
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 474, 510, 760
BssMI GATC 2 cut(s) 23, 310
BssNI GRCGYC 1 cut(s) 674
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 255, 762
Bst4CI ACNGT 3 cut(s) 245, 506, 571
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 192, 387
BstDEI CTNAG 2 cut(s) 151, 237
BstEII GGTNACC 1 cut(s) 652
BstF5I GGATG 2 cut(s) 329, 520
BstKTI GATC 2 cut(s) 26, 313
BstMAI GTCTC 2 cut(s) 428, 665
BstMBI GATC 2 cut(s) 23, 310
BstMWI GCNNNNNNNGC 6 cut(s) 11, 293, 302, 347, 356, 704
BstNI CCWGG 2 cut(s) 255, 762
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 3 cut(s) 19, 253, 760
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 7 cut(s) 26, 47, 245, 334, 337, 621, 707
BsuRI GGCC 3 cut(s) 5, 190, 646
BtsCI GGATG 2 cut(s) 329, 520
BtsIMutI CAGTG 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 192, 387
CaiI CAGNNNCTG 1 cut(s) 191
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 188, 555, 644
Csp6I GTAC 3 cut(s) 99, 375, 782
CviAII CATG 5 cut(s) 7, 31, 81, 314, 378
CviQI GTAC 3 cut(s) 99, 375, 782
DdeI CTNAG 2 cut(s) 151, 237
DpnI GATC 2 cut(s) 25, 312
DpnII GATC 2 cut(s) 23, 310
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Eco130I CCWWGG 1 cut(s) 474
Eco32I GATATC 2 cut(s) 619, 689
Eco47I GGWCC 1 cut(s) 555
Eco57I CTGAAG 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 652
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 253, 760
EcoRV GATATC 2 cut(s) 619, 689
EcoT14I CCWWGG 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 5 cut(s) 10, 34, 84, 317, 381
FaqI GGGAC 1 cut(s) 492
FatI CATG 5 cut(s) 6, 30, 80, 313, 377
FauNDI CATATG 1 cut(s) 721
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 7 cut(s) 15, 36, 234, 348, 351, 610, 696
FokI GGATG 2 cut(s) 336, 527
Fsp4HI GCNGC 7 cut(s) 15, 36, 234, 348, 351, 610, 696
FspBI CTAG 2 cut(s) 368, 847
GluI GCNGC 7 cut(s) 15, 36, 234, 348, 351, 610, 696
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 3 cut(s) 5, 190, 646
HapII CCGG 2 cut(s) 21, 386
Hin1I GRCGYC 1 cut(s) 674
Hin1II CATG 5 cut(s) 10, 34, 84, 317, 381
HinfI GANTC 2 cut(s) 404, 587
HpaII CCGG 2 cut(s) 21, 386
Hpy166II GTNNAC 3 cut(s) 248, 481, 628
Hpy188III TCNNGA 4 cut(s) 228, 509, 521, 678
Hpy8I GTNNAC 3 cut(s) 248, 481, 628
HpyAV CCTTC 3 cut(s) 120, 199, 814
HpyCH4III ACNGT 3 cut(s) 245, 506, 571
HpyCH4IV ACGT 1 cut(s) 674
HpyCH4V TGCA 6 cut(s) 14, 51, 84, 494, 695, 707
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 293, 302, 347, 356, 704
HpyF3I CTNAG 2 cut(s) 151, 237
HpySE526I ACGT 1 cut(s) 674
Hsp92I GRCGYC 1 cut(s) 674
Hsp92II CATG 5 cut(s) 10, 34, 84, 317, 381
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 23, 310
LguI GCTCTTC 1 cut(s) 824
LmnI GCTCC 3 cut(s) 22, 230, 751
Lsp1109I GCAGC 7 cut(s) 26, 47, 245, 334, 337, 621, 707
LweI GCATC 2 cut(s) 481, 716
MaeI CTAG 2 cut(s) 368, 847
MaeII ACGT 1 cut(s) 674
MaeIII GTNAC 3 cut(s) 211, 652, 713
MalI GATC 2 cut(s) 25, 312
MbiI CCGCTC 1 cut(s) 817
MboI GATC 2 cut(s) 23, 310
MboII GAAGA 3 cut(s) 132, 536, 811
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 363, 468, 562, 823
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 413
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 385
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 42, 132, 413, 573
MslI CAYNNNNRTG 1 cut(s) 318
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 38, 236
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 2 cut(s) 21, 386
MspR9I CCNGG 3 cut(s) 21, 255, 762
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 2 cut(s) 255, 762
MwoI GCNNNNNNNGC 6 cut(s) 11, 293, 302, 347, 356, 704
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 21
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 23, 310
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 10, 34, 84, 317, 381
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 211
PciSI GCTCTTC 1 cut(s) 824
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 19
PkrI GCNGC 7 cut(s) 16, 37, 235, 349, 352, 611, 697
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PpuMI RGGWCCY 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 253, 760
PspEI GGTNACC 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 2 cut(s) 253, 760
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 3 cut(s) 188, 555, 644
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 3 cut(s) 100, 376, 783
RsaNI GTAC 3 cut(s) 99, 375, 782
RseI CAYNNNNRTG 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 824
SaqAI TTAA 4 cut(s) 42, 132, 413, 573
SatI GCNGC 7 cut(s) 15, 36, 234, 348, 351, 610, 696
Sau3AI GATC 2 cut(s) 23, 310
Sau96I GGNCC 3 cut(s) 188, 555, 644
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 3 cut(s) 21, 255, 762
SfaNI GCATC 2 cut(s) 481, 716
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 1 cut(s) 555
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 2 cut(s) 131, 734
SmoI CTYRAG 2 cut(s) 131, 734
Sse9I AATT 4 cut(s) 363, 468, 562, 823
SsiI CCGC 2 cut(s) 38, 815
SspMI CTAG 2 cut(s) 368, 847
StyD4I CCNGG 3 cut(s) 19, 253, 760
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 3 cut(s) 245, 506, 571
TaiI ACGT 1 cut(s) 677
TasI AATT 4 cut(s) 363, 468, 562, 823
TatI WGTACW 1 cut(s) 781
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 4 cut(s) 42, 132, 413, 573
Tru9I TTAA 4 cut(s) 42, 132, 413, 573
TscAI CASTG 1 cut(s) 396
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 7 cut(s) 14, 35, 233, 347, 350, 609, 695
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 2 cut(s) 280, 770
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 396
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 555
XapI RAATTY 1 cut(s) 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 664
XspI CTAG 2 cut(s) 368, 847
ZraI GACGTC 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.