Rroxscaffold_6G00401960

Enoyl- acyl-carrier-protein reductase NADH 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001646
Physical Location & Seq
Reverse (-)
24356916 .. 24361685
4770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00401960.1

Sequence Viewer

Length: 1182 bp
ATGGCAACTACTGCAGCTCCTGGTTCGCACGTGGCAGCAATTAAGCCTTGCATAACTTCTTCCCGAAGAACATACAAGTCAGGTACTGCCAATTTTGGTGCAAACAGTGAAGTGGCAGTTTCTAAGGGGCTTAGAAGCTCTTCCCACATCTCATCAAGGCAACCATTCTTCCAGAGCATCCAATCAGGCCCTGTGAAGTCTGTAAAGGTTATCACAAGAGCGATGTCAGCAGCAGCTGAGAACAGTCCATTGCCAGGATTGCCTATTGATTTGAGAGGAAAGAGAGCATTCATTGCTGGTGTGGCTGATGATAATGGATATGGATGGGCAATAGCAAAACAACTTGCTGGTGCTGGTGCTGAAATTCTTCTTGGTACATGGGTGCCGGCACTGAACATTTTCGAGTCCAGTTTAAGGCGTGGGAAGTTTGATGAATCACGCATATTGCCCGACGGTTCTTTGATGGAAATTACAAAAGTCTATCCATTGGATGCAGTTTTTGACAATCCTGATGATGTTCCTGAAGATATAAAATCAAACAAACGCTACTTGGGGTCCTCAAAGTGGACGGTTAAGGAGGTTGCTGAATCTGTTAAAGAGGATTTTGGCAGCATTGATATCCTAGTCCATTCCCTGGCTAATGGACCAGAGGTTGTCAAACCTCTTATGGAAACGTCAAGATATGGATATCTTGCAGCTATCTCTGCATCAAGCTACTCGTTTATTTCTTTACTCCAGCATTTTGCTCCAATAATGAACCCAGGCACGTGGTGCGTCAATCTCTCTGACATACATTGCTTCCGAGAGGATCATTCCCGGATATGGTGGAGGTATGAGTTCAGCGAAGGCTGCTCTAGAGAGAGTGACACACGTGTGCTGGCCTTTGAAGCTGGGAGAAAACACAGAATCAGAGTCAACACAATATCTGCAGGCCCATTAAGAAGTCGTGCTGCAAAAGCTATTGGTTTTATCGATATGATGATTGATTACTCATCTGCCAATGCTCCCTTACCGAAAGAGTTATCTGCTGAGGAGGTGGGGAACGTTGCTGCCTTCCTTGCATCACCATTGGCATCAGCAATCACCGGCAGTGTTGTATACGTTGACAATGGTCTAAATGCAATGGGTGTGGGAGTTGACAGCCCAATTTTTGAAAACCTTGAGCTTCCAAAAGAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00333 ko00780 ko01100 ko01130 ko01212 map00061 map00333 map00780 map01100 map01130 map01212
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

393

Amino Acids

42.39

Weight (kDa)

6.68

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 190 - 373 1.8e-37 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 382
AccB7I CCANNNNNTGG 1 cut(s) 634
AccI GTMKAC 1 cut(s) 1098
AclI AACGTT 1 cut(s) 1044
AclWI GGATC 1 cut(s) 816
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 543
AcvI CACGTG 3 cut(s) 31, 768, 872
AdeI CACNNNGTG 1 cut(s) 771
AfaI GTAC 2 cut(s) 85, 376
AfiI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 822
AflIII ACRYGT 2 cut(s) 869, 871
AgsI TTSAA 2 cut(s) 887, 1154
AjnI CCWGG 4 cut(s) 19, 253, 633, 760
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 1 cut(s) 872
AluBI AGCT 8 cut(s) 17, 138, 236, 698, 714, 890, 959, 1165
AluI AGCT 8 cut(s) 17, 138, 236, 698, 714, 890, 959, 1165
AlwI GGATC 1 cut(s) 816
AlwNI CAGNNNCTG 4 cut(s) 20, 86, 191, 236
AoxI GGCC 3 cut(s) 187, 879, 931
ApeKI GCWGC 9 cut(s) 14, 35, 230, 233, 609, 695, 849, 950, 1049
ApoI RAATTY 1 cut(s) 363
Asp700I GAANNNNTTC 3 cut(s) 139, 366, 398
AspS9I GGNCC 4 cut(s) 188, 555, 644, 932
AsuC2I CCSGG 1 cut(s) 817
AsuHPI GGTGA 2 cut(s) 1056, 1075
AvaII GGWCC 2 cut(s) 555, 644
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BanI GGYRCC 1 cut(s) 382
BbrPI CACGTG 3 cut(s) 31, 768, 872
BbvCI CCTCAGC 1 cut(s) 1029
BbvI GCAGC 9 cut(s) 26, 47, 242, 245, 621, 707, 836, 937, 1036
BccI CCATC 2 cut(s) 318, 457
BciT130I CCWGG 4 cut(s) 21, 255, 635, 762
BcnI CCSGG 1 cut(s) 817
BfaI CTAG 3 cut(s) 623, 855, 1180
BfmI CTRYAG 2 cut(s) 12, 927
BisI GCNGC 9 cut(s) 15, 36, 231, 234, 610, 696, 850, 951, 1050
BlsI GCNGC 9 cut(s) 16, 37, 232, 235, 611, 697, 851, 952, 1051
Bme1390I CCNGG 5 cut(s) 21, 255, 635, 762, 817
Bme18I GGWCC 2 cut(s) 555, 644
BmgT120I GGNCC 4 cut(s) 188, 555, 644, 932
BmiI GGNNCC 2 cut(s) 384, 556
BmrFI CCNGG 5 cut(s) 21, 255, 635, 762, 817
BmsI GCATC 5 cut(s) 186, 481, 716, 1070, 1082
BoxI GACNNNNGTC 1 cut(s) 1110
BpmI CTGGAG 1 cut(s) 719
Bpu10I CCTNAGC 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 1 cut(s) 817
Bsa29I ATCGAT 1 cut(s) 972
BsaAI YACGTR 3 cut(s) 31, 768, 872
BsaJI CCNNGG 2 cut(s) 633, 760
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 822
Bse118I RCCGGY 2 cut(s) 385, 1085
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 4 cut(s) 248, 291, 793, 1128
BseBI CCWGG 4 cut(s) 21, 255, 635, 762
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 633, 760
BseGI GGATG 3 cut(s) 177, 329, 496
BseLI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 822
BseMI GCAATG 4 cut(s) 248, 291, 793, 1128
BseMII CTCAG 2 cut(s) 228, 1020
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 1046
BseXI GCAGC 9 cut(s) 26, 47, 242, 245, 621, 707, 836, 937, 1036
BseYI CCCAGC 1 cut(s) 890
BshFI GGCC 3 cut(s) 189, 881, 933
BshNI GGYRCC 1 cut(s) 382
BshVI ATCGAT 1 cut(s) 972
BsiSI CCGG 3 cut(s) 386, 817, 1086
BslI CCNNNNNNNGG 5 cut(s) 254, 414, 564, 634, 822
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 3 cut(s) 189, 881, 933
Bsp143I GATC 1 cut(s) 808
BspANI GGCC 3 cut(s) 189, 881, 933
BspCNI CTCAG 2 cut(s) 229, 1021
BspDI ATCGAT 1 cut(s) 972
BspLI GGNNCC 2 cut(s) 384, 556
BspMAI CTGCAG 2 cut(s) 16, 931
BspPI GGATC 1 cut(s) 816
BspQI GCTCTTC 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 382
BsrDI GCAATG 4 cut(s) 248, 291, 793, 1128
BsrFI RCCGGY 2 cut(s) 385, 1085
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 2 cut(s) 385, 1085
BssECI CCNNGG 2 cut(s) 633, 760
BssMI GATC 1 cut(s) 808
BssNAI GTATAC 1 cut(s) 1099
Bst1107I GTATAC 1 cut(s) 1099
Bst2UI CCWGG 4 cut(s) 21, 255, 635, 762
Bst4CI ACNGT 4 cut(s) 107, 245, 455, 571
Bst6I CTCTTC 1 cut(s) 145
BstAPI GCANNNNNTGC 3 cut(s) 11, 293, 771
BstBAI YACGTR 3 cut(s) 31, 768, 872
BstC8I GCNNGC 3 cut(s) 387, 879, 931
BstDEI CTNAG 4 cut(s) 123, 131, 237, 1029
BstF5I GGATG 3 cut(s) 177, 329, 496
BstKTI GATC 1 cut(s) 811
BstMBI GATC 1 cut(s) 808
BstNI CCWGG 4 cut(s) 21, 255, 635, 762
BstPAI GACNNNNGTC 1 cut(s) 1110
BstSCI CCNGG 5 cut(s) 19, 253, 633, 760, 815
BstSFI CTRYAG 2 cut(s) 12, 927
BstV1I GCAGC 9 cut(s) 26, 47, 242, 245, 621, 707, 836, 937, 1036
BstXI CCANNNNNNTGG 1 cut(s) 768
BstZ17I GTATAC 1 cut(s) 1099
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 3 cut(s) 189, 881, 933
BsuTUI ATCGAT 1 cut(s) 972
BtgZI GCGATG 1 cut(s) 236
BtsCI GGATG 3 cut(s) 177, 329, 496
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 3 cut(s) 112, 389, 1096
Cac8I GCNNGC 3 cut(s) 387, 879, 931
CaiI CAGNNNCTG 4 cut(s) 20, 86, 191, 236
Cfr10I RCCGGY 2 cut(s) 385, 1085
Cfr13I GGNCC 4 cut(s) 188, 555, 644, 932
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 1 cut(s) 763
Csp6I GTAC 2 cut(s) 84, 375
CspCI CAANNNNNGTGG 2 cut(s) 1110, 1145
CviAII CATG 1 cut(s) 378
CviQI GTAC 2 cut(s) 84, 375
DdeI CTNAG 4 cut(s) 123, 131, 237, 1029
DpnI GATC 1 cut(s) 810
DpnII GATC 1 cut(s) 808
DraIII CACNNNGTG 1 cut(s) 771
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco32I GATATC 2 cut(s) 619, 689
Eco47I GGWCC 2 cut(s) 555, 644
Eco57I CTGAAG 1 cut(s) 543
Eco72I CACGTG 3 cut(s) 31, 768, 872
EcoO109I RGGNCCY 2 cut(s) 188, 555
EcoRII CCWGG 4 cut(s) 19, 253, 633, 760
EcoRV GATATC 2 cut(s) 619, 689
FaeI CATG 1 cut(s) 381
FatI CATG 1 cut(s) 377
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 9 cut(s) 15, 36, 231, 234, 610, 696, 850, 951, 1050
FokI GGATG 3 cut(s) 164, 336, 503
Fsp4HI GCNGC 9 cut(s) 15, 36, 231, 234, 610, 696, 850, 951, 1050
FspBI CTAG 3 cut(s) 623, 855, 1180
GluI GCNGC 9 cut(s) 15, 36, 231, 234, 610, 696, 850, 951, 1050
GsaI CCCAGC 1 cut(s) 894
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 3 cut(s) 189, 881, 933
HapII CCGG 3 cut(s) 386, 817, 1086
HgaI GACGC 1 cut(s) 763
Hin1II CATG 1 cut(s) 381
HincII GTYRAC 3 cut(s) 916, 1105, 1138
HindII GTYRAC 3 cut(s) 916, 1105, 1138
HinfI GANTC 5 cut(s) 404, 434, 587, 906, 912
HpaII CCGG 3 cut(s) 386, 817, 1086
HphI GGTGA 2 cut(s) 1056, 1075
Hpy166II GTNNAC 5 cut(s) 567, 916, 1099, 1105, 1138
Hpy188I TCNGA 3 cut(s) 787, 803, 911
Hpy188III TCNNGA 6 cut(s) 63, 172, 509, 521, 678, 855
Hpy8I GTNNAC 5 cut(s) 567, 916, 1099, 1105, 1138
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 2 cut(s) 839, 1063
HpyCH4III ACNGT 4 cut(s) 107, 245, 455, 571
HpyCH4IV ACGT 6 cut(s) 30, 674, 767, 871, 1044, 1101
HpyF3I CTNAG 4 cut(s) 123, 131, 237, 1029
HpySE526I ACGT 6 cut(s) 30, 674, 767, 871, 1044, 1101
Hsp92II CATG 1 cut(s) 381
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 808
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 3 cut(s) 22, 751, 1009
Lsp1109I GCAGC 9 cut(s) 26, 47, 242, 245, 621, 707, 836, 937, 1036
LweI GCATC 5 cut(s) 186, 481, 716, 1070, 1082
MaeI CTAG 3 cut(s) 623, 855, 1180
MaeII ACGT 6 cut(s) 30, 674, 767, 871, 1044, 1101
MaeIII GTNAC 1 cut(s) 863
MalI GATC 1 cut(s) 810
MboI GATC 1 cut(s) 808
MboII GAAGA 6 cut(s) 51, 78, 132, 160, 359, 536
MluCI AATT 5 cut(s) 39, 91, 363, 468, 1146
MlyI GAGTC 2 cut(s) 413, 921
MroNI GCCGGC 1 cut(s) 385
MroXI GAANNNNTTC 3 cut(s) 139, 366, 398
MseI TTAA 5 cut(s) 42, 413, 573, 594, 938
MslI CAYNNNNRTG 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 3 cut(s) 386, 817, 1086
MspR9I CCNGG 5 cut(s) 21, 255, 635, 762, 817
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 4 cut(s) 21, 255, 635, 762
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 817
NdeII GATC 1 cut(s) 808
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 1 cut(s) 381
NlaIV GGNNCC 2 cut(s) 384, 556
NmuCI GTSAC 1 cut(s) 863
OliI CACNNNNGTG 1 cut(s) 872
PciSI GCTCTTC 1 cut(s) 145
PctI GAATGC 1 cut(s) 287
PdiI GCCGGC 1 cut(s) 387
PdmI GAANNNNTTC 3 cut(s) 139, 366, 398
PfeI GAWTC 3 cut(s) 434, 587, 906
PflMI CCANNNNNTGG 1 cut(s) 634
PfoI TCCNGGA 1 cut(s) 815
PkrI GCNGC 9 cut(s) 16, 37, 232, 235, 611, 697, 851, 952, 1051
PleI GAGTC 2 cut(s) 412, 920
PmaCI CACGTG 3 cut(s) 31, 768, 872
PmlI CACGTG 3 cut(s) 31, 768, 872
PpsI GAGTC 2 cut(s) 412, 920
Ppu21I YACGTR 3 cut(s) 31, 768, 872
PpuMI RGGWCCY 1 cut(s) 555
PshAI GACNNNNGTC 1 cut(s) 1110
Psp1406I AACGTT 1 cut(s) 1044
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 4 cut(s) 19, 253, 633, 760
PspCI CACGTG 3 cut(s) 31, 768, 872
PspFI CCCAGC 1 cut(s) 890
PspGI CCWGG 4 cut(s) 19, 253, 633, 760
PspN4I GGNNCC 2 cut(s) 384, 556
PspPI GGNCC 4 cut(s) 188, 555, 644, 932
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 2 cut(s) 16, 931
PstNI CAGNNNCTG 4 cut(s) 20, 86, 191, 236
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 85, 376
RsaNI GTAC 2 cut(s) 84, 375
RseI CAYNNNNRTG 1 cut(s) 872
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 5 cut(s) 42, 413, 573, 594, 938
SatI GCNGC 9 cut(s) 15, 36, 231, 234, 610, 696, 850, 951, 1050
Sau3AI GATC 1 cut(s) 808
Sau96I GGNCC 4 cut(s) 188, 555, 644, 932
SchI GAGTC 2 cut(s) 413, 921
ScrFI CCNGG 5 cut(s) 21, 255, 635, 762, 817
SfaNI GCATC 5 cut(s) 186, 481, 716, 1070, 1082
SfcI CTRYAG 2 cut(s) 12, 927
SinI GGWCC 2 cut(s) 555, 644
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 1160
SmoI CTYRAG 1 cut(s) 1160
Sse9I AATT 5 cut(s) 39, 91, 363, 468, 1146
SspMI CTAG 3 cut(s) 623, 855, 1180
StyD4I CCNGG 5 cut(s) 19, 253, 633, 760, 815
TaaI ACNGT 4 cut(s) 107, 245, 455, 571
TaiI ACGT 6 cut(s) 33, 677, 770, 874, 1047, 1104
TaqI TCGA 2 cut(s) 402, 972
TasI AATT 5 cut(s) 39, 91, 363, 468, 1146
TfiI GAWTC 3 cut(s) 434, 587, 906
Tru1I TTAA 5 cut(s) 42, 413, 573, 594, 938
Tru9I TTAA 5 cut(s) 42, 413, 573, 594, 938
TscAI CASTG 3 cut(s) 112, 396, 1096
TseFI GTSAC 1 cut(s) 863
TseI GCWGC 9 cut(s) 14, 35, 230, 233, 609, 695, 849, 950, 1049
Tsp45I GTSAC 1 cut(s) 863
TspDTI ATGAA 3 cut(s) 280, 447, 770
TspRI CASTG 3 cut(s) 112, 396, 1096
Van91I CCANNNNNTGG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 555, 644
XapI RAATTY 1 cut(s) 363
XbaI TCTAGA 1 cut(s) 854
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 3 cut(s) 139, 366, 398
XspI CTAG 3 cut(s) 623, 855, 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.