MD12G1167400.v1.1

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003984
Physical Location & Seq
Forward (+)
24768274 .. 24769750
1477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1167400.v1.1.491

Sequence Viewer

Length: 876 bp
ATGCCTACAAGGTATGTTTTGAGTGATCCCAAAAAACGAGTGGTGTACGATCAGCAGGGCAATGAGGGTTTGAGGAGAGGGGCAACGCAGCCTTCTCATTCACCCAGGTCTGGCCGCATGAACGGCAAGTGCCCTGACCCATTCACACAATTTTTCGGGGCATGGACTGACATGGCTCAGGTTACGTCTCGGGTTATGGAGTCCGTGGAACCAGAAGAGATATTCTCGATGTTTTTAGTTGGAGAGACGATAGGGGAAAATGGATTCCAATGGTTTGAATGCACGGACTCAGGACCTGGAAATGGGTCGAGGAAAGCCGTGACGATTAAGAGGACATTACCTTGTACTTTGGAGGAACTGTATAAGGGCGCCACCAAGAAGCTAAAGATTTCTAGGGATGTTCTTGGTGTTAATGGAAGAAAAAGCACAGTGGAGGAAGTTCTAATAATTGAGATCAAGCCAGGGTGGAAAAAGGGCACGAAAATCACTTTCCCTGAGGAGGGGCACGACACACGGCGAGGTGTGATACCAACAGACATTGTCTTCATCGTCGATGAAAAGCCTCATGGTGTTTACAAGAGAGACGAGGACGATCTGATTGTCACTCAGAATATCTCTCTAGCGGAGGCTCTGACGGGTCACATCGCGCAGCTGACAGCCCTAGACGGGAGAAAATTGAGCGTCTCCATTGGTTCCATTATCAGCCAGACCCATGAAGGAGTGATTATAGGGGAGGGGATGCTTATCCAAAAGGAACAATCCAAGAAAGGGAACTTGATTGTTAAGTTCAACGTCAAGTTCCCCAAGCTGACCCCGAAGCAGAAAACAAGCATCAGAATGTTGCTTTTTATTTTTATTTTGTTTTATTTTTTTTGA

Protein Analysis

292

Amino Acids

32.62

Weight (kDa)

9.2

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 110 - 268 5.5e-35 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 368
AccII CGCG 1 cut(s) 647
AciI CCGC 2 cut(s) 115, 623
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 112
AcyI GRCGYC 1 cut(s) 369
AfaI GTAC 2 cut(s) 47, 346
AfiI CCNNNNNNNGG 6 cut(s) 110, 302, 499, 500, 666, 768
AgsI TTSAA 2 cut(s) 278, 790
AjnI CCWGG 3 cut(s) 104, 295, 460
AluBI AGCT 3 cut(s) 382, 652, 808
AluI AGCT 3 cut(s) 382, 652, 808
Alw26I GTCTC 4 cut(s) 192, 239, 576, 688
AlwI GGATC 1 cut(s) 20
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 88, 649
AspLEI GCGC 2 cut(s) 371, 649
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 93
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 1 cut(s) 293
AxyI CCTNAGG 1 cut(s) 495
BaeGI GKGCMC 3 cut(s) 134, 479, 507
BanI GGYRCC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 535
BbvI GCAGC 2 cut(s) 100, 661
BceAI ACGGC 3 cut(s) 139, 302, 530
BciT130I CCWGG 3 cut(s) 106, 297, 462
BcoDI GTCTC 4 cut(s) 192, 239, 576, 688
BfaI CTAG 3 cut(s) 393, 620, 662
BfoI RGCGCY 1 cut(s) 372
BisI GCNGC 3 cut(s) 89, 115, 650
BlsI GCNGC 3 cut(s) 90, 116, 651
Bme1390I CCNGG 3 cut(s) 106, 297, 462
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 3 cut(s) 210, 370, 694
BmrFI CCNGG 3 cut(s) 106, 297, 462
BmsI GCATC 2 cut(s) 729, 840
BpiI GAAGAC 1 cut(s) 535
BplI GAGNNNNNCTC 2 cut(s) 209, 241
Bpu10I CCTNAGC 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 743
BsaHI GRCGYC 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 104, 204, 461
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 6 cut(s) 110, 302, 499, 500, 666, 768
Bse21I CCTNAGG 1 cut(s) 495
Bse3DI GCAATG 1 cut(s) 67
Bse8I GATNNNNATC 1 cut(s) 743
BseBI CCWGG 3 cut(s) 106, 297, 462
BseDI CCNNGG 3 cut(s) 104, 204, 461
BseGI GGATG 2 cut(s) 403, 744
BseJI GATNNNNATC 1 cut(s) 743
BseLI CCNNNNNNNGG 6 cut(s) 110, 302, 499, 500, 666, 768
BseMI GCAATG 1 cut(s) 67
BseMII CTCAG 4 cut(s) 191, 303, 486, 620
BseRI GAGGAG 2 cut(s) 88, 512
BseSI GKGCMC 3 cut(s) 134, 479, 507
BseXI GCAGC 2 cut(s) 100, 661
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 368
BsiHKCI CYCGRG 1 cut(s) 189
BslI CCNNNNNNNGG 6 cut(s) 110, 302, 499, 500, 666, 768
BsmAI GTCTC 4 cut(s) 192, 239, 576, 688
BsmBI CGTCTC 4 cut(s) 192, 239, 576, 688
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 1 cut(s) 114
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 3 cut(s) 134, 479, 507
Bsp143I GATC 4 cut(s) 25, 49, 453, 592
BspACI CCGC 2 cut(s) 115, 623
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 4 cut(s) 190, 302, 487, 619
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 3 cut(s) 210, 370, 694
BspPI GGATC 1 cut(s) 20
BspT107I GGYRCC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 104, 204, 461
BssMI GATC 4 cut(s) 25, 49, 453, 592
BssNI GRCGYC 1 cut(s) 369
Bst2UI CCWGG 3 cut(s) 106, 297, 462
Bst4CI ACNGT 2 cut(s) 360, 430
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 369
BstDEI CTNAG 4 cut(s) 177, 289, 495, 606
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 403, 744
BstFNI CGCG 1 cut(s) 647
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 371, 649
BstKTI GATC 4 cut(s) 28, 52, 456, 595
BstMAI GTCTC 4 cut(s) 192, 239, 576, 688
BstMBI GATC 4 cut(s) 25, 49, 453, 592
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstNI CCWGG 3 cut(s) 106, 297, 462
BstSCI CCNGG 3 cut(s) 104, 295, 460
BstSLI GKGCMC 3 cut(s) 134, 479, 507
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 2 cut(s) 100, 661
BstV2I GAAGAC 1 cut(s) 535
Bsu36I CCTNAGG 1 cut(s) 495
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 628
BtsCI GGATG 2 cut(s) 403, 744
BtsIMutI CAGTG 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 2 cut(s) 371, 649
Cfr13I GGNCC 1 cut(s) 293
CseI GACGC 1 cut(s) 670
Csp6I GTAC 2 cut(s) 46, 345
CviAII CATG 5 cut(s) 118, 162, 172, 566, 713
CviQI GTAC 2 cut(s) 46, 345
DdeI CTNAG 4 cut(s) 177, 289, 495, 606
DinI GGCGCC 1 cut(s) 370
DpnI GATC 4 cut(s) 27, 51, 455, 594
DpnII GATC 4 cut(s) 25, 49, 453, 592
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 293
Eco81I CCTNAGG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 104, 295, 460
EgeI GGCGCC 1 cut(s) 370
EheI GGCGCC 1 cut(s) 370
Esp3I CGTCTC 4 cut(s) 192, 239, 576, 688
FaeI CATG 5 cut(s) 121, 165, 175, 569, 716
FaiI YATR 9 cut(s) 15, 119, 163, 173, 197, 363, 567, 714, 728
FatI CATG 5 cut(s) 117, 161, 171, 565, 712
Fnu4HI GCNGC 3 cut(s) 89, 115, 650
FokI GGATG 2 cut(s) 410, 751
Fsp4HI GCNGC 3 cut(s) 89, 115, 650
FspBI CTAG 3 cut(s) 393, 620, 662
GlaI GCGC 2 cut(s) 370, 648
GluI GCNGC 3 cut(s) 89, 115, 650
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 1 cut(s) 114
HgaI GACGC 1 cut(s) 670
HhaI GCGC 2 cut(s) 371, 649
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 5 cut(s) 121, 165, 175, 569, 716
Hin6I GCGC 2 cut(s) 369, 647
HinP1I GCGC 2 cut(s) 369, 647
HinfI GANTC 3 cut(s) 200, 264, 287
HphI GGTGA 1 cut(s) 93
Hpy166II GTNNAC 2 cut(s) 46, 574
Hpy188I TCNGA 4 cut(s) 597, 609, 633, 836
Hpy188III TCNNGA 2 cut(s) 226, 291
Hpy8I GTNNAC 2 cut(s) 46, 574
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 2 cut(s) 102, 710
HpyCH4III ACNGT 2 cut(s) 360, 430
HpyCH4IV ACGT 2 cut(s) 185, 792
HpyCH4V TGCA 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpyF3I CTNAG 4 cut(s) 177, 289, 495, 606
HpySE526I ACGT 2 cut(s) 185, 792
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 5 cut(s) 121, 165, 175, 569, 716
HspAI GCGC 2 cut(s) 369, 647
KasI GGCGCC 1 cut(s) 368
Kzo9I GATC 4 cut(s) 25, 49, 453, 592
Lsp1109I GCAGC 2 cut(s) 100, 661
LweI GCATC 2 cut(s) 729, 840
MaeI CTAG 3 cut(s) 393, 620, 662
MaeII ACGT 2 cut(s) 185, 792
MaeIII GTNAC 4 cut(s) 181, 319, 601, 638
MalI GATC 4 cut(s) 27, 51, 455, 594
MboI GATC 4 cut(s) 25, 49, 453, 592
MboII GAAGA 3 cut(s) 227, 429, 535
MhlI GDGCHC 3 cut(s) 134, 479, 507
MluCI AATT 3 cut(s) 149, 447, 674
Mly113I GGCGCC 1 cut(s) 369
MlyI GAGTC 2 cut(s) 209, 281
MmeI TCCRAC 1 cut(s) 220
MseI TTAA 3 cut(s) 327, 411, 783
MslI CAYNNNNRTG 1 cut(s) 836
MspA1I CMGCKG 1 cut(s) 652
MspR9I CCNGG 3 cut(s) 106, 297, 462
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 3 cut(s) 106, 297, 462
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 123
NarI GGCGCC 1 cut(s) 369
NdeII GATC 4 cut(s) 25, 49, 453, 592
NlaIII CATG 5 cut(s) 121, 165, 175, 569, 716
NlaIV GGNNCC 3 cut(s) 210, 370, 694
NmuCI GTSAC 3 cut(s) 319, 601, 638
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 264
PflFI GACNNNGTC 1 cut(s) 539
PkrI GCNGC 3 cut(s) 90, 116, 651
PleI GAGTC 2 cut(s) 208, 281
PluTI GGCGCC 1 cut(s) 372
PpsI GAGTC 2 cut(s) 208, 281
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 3 cut(s) 104, 295, 460
PspGI CCWGG 3 cut(s) 104, 295, 460
PspN4I GGNNCC 3 cut(s) 210, 370, 694
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 652
RsaI GTAC 2 cut(s) 47, 346
RsaNI GTAC 2 cut(s) 46, 345
RseI CAYNNNNRTG 1 cut(s) 836
SaqAI TTAA 3 cut(s) 327, 411, 783
SatI GCNGC 3 cut(s) 89, 115, 650
Sau3AI GATC 4 cut(s) 25, 49, 453, 592
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 2 cut(s) 209, 281
ScrFI CCNGG 3 cut(s) 106, 297, 462
SduI GDGCHC 3 cut(s) 134, 479, 507
SfaNI GCATC 2 cut(s) 729, 840
SfoI GGCGCC 1 cut(s) 370
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 836
Sse9I AATT 3 cut(s) 149, 447, 674
SsiI CCGC 2 cut(s) 115, 623
SspDI GGCGCC 1 cut(s) 368
SspMI CTAG 3 cut(s) 393, 620, 662
StyD4I CCNGG 3 cut(s) 104, 295, 460
TaaI ACNGT 2 cut(s) 360, 430
TaiI ACGT 2 cut(s) 188, 795
TaqI TCGA 3 cut(s) 227, 308, 552
TasI AATT 3 cut(s) 149, 447, 674
TatI WGTACW 1 cut(s) 344
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 3 cut(s) 327, 411, 783
Tru9I TTAA 3 cut(s) 327, 411, 783
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 3 cut(s) 319, 601, 638
TseI GCWGC 2 cut(s) 88, 649
Tsp45I GTSAC 3 cut(s) 319, 601, 638
TspDTI ATGAA 4 cut(s) 134, 535, 570, 729
TspGWI ACGGA 2 cut(s) 193, 299
TspRI CASTG 1 cut(s) 435
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 293
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 3 cut(s) 393, 620, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.