MD12G1178000.v1.1

APO protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002143
Physical Location & Seq
Forward (+)
25868202 .. 25869263
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1178000.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCACTGAGGAAGAAATTGTGCGAAGGATCCAATTTCAATGGCAAAATTATGGACAACTTTGCAAGGTATTACAGCTCGAAGATGGACTTAAGGAAGCTAAAGCCTATGATTCAAAAGAGAATCCAAAACCGAGCTAAAGATTACCTAGTGCCGGCCATGGTACCAGTTGCCCAGGAGGTCTTTAATTCCCGGAGGCTCGTTATGCAAGGTGTCTCTACTCTCCTCAAAATGGTACCAGTTTTGGCCTGCAAGAGCAATTTATGTGACTCAACGAAAGGCCACTTAATTCAGACTTGTTGTGGCTTCAAACGTCGTGGCAAAAATCGAGTTCATGAATGGATAGTTGGTGGTTTGAAAGATGTGCTTGTTCCAGTAGAGGCATTTCACCTCAAGCACATGTTCCAGAATGTTATAAAACACCATCAGAGGTTTGACTTTGAACGTGTTCATGCAGTTGTCGAGCTATGCTGGCAAGCAGGGGCAAATGATGGAAATTATTTAAATTGGAACTCTGAAAGTGATTGTGTTGGCGTTGATGGAGCTGAGTCTTTGTCATCTGATGAACTGATTTTGGTTGCCAGTGGAACTTTGAGGGCATGGGAAATACTTAGAAATGGCGTGGAGAAGTTGCTGATGGTTTATCCAGCGAAAGTCTGTAAACATTGTTCAGAAGTTCATGTTGGGCCATCTGGGCATAAGGCTAGGCTTTGTGGAATTTTTAAATATGAGAGTTGGCAGGGAACCCATTTTTGGAGGAAGGCAAATGTAGATGATCTTGTACCTCCAAAGACTGTGGCGGCGGCGTCCTCAGGATCCTCAAGTACTTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

279

Amino Acids

31.31

Weight (kDa)

9.33

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 27 - 163 4.6e-34 APO RNA-binding
APO_RNA-bind PF05634 187 - 263 3e-15 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
Acc65I GGTACC 2 cut(s) 163, 235
AccB1I GGYRCC 2 cut(s) 163, 235
AciI CCGC 2 cut(s) 800, 803
AclWI GGATC 4 cut(s) 24, 37, 810, 823
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 1 cut(s) 717
AcyI GRCGYC 1 cut(s) 806
AfaI GTAC 4 cut(s) 165, 237, 783, 826
AfiI CCNNNNNNNGG 3 cut(s) 154, 232, 753
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 2 cut(s) 399, 445
AgsI TTSAA 6 cut(s) 40, 116, 310, 358, 443, 833
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 6 cut(s) 330, 362, 666, 698, 736, 768
AluBI AGCT 5 cut(s) 78, 100, 137, 466, 545
AluI AGCT 5 cut(s) 78, 100, 137, 466, 545
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 4 cut(s) 24, 37, 810, 823
AoxI GGCC 4 cut(s) 156, 246, 280, 686
ApoI RAATTY 1 cut(s) 717
Asp700I GAANNNNTTC 1 cut(s) 447
Asp718I GGTACC 2 cut(s) 163, 235
AspS9I GGNCC 1 cut(s) 686
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 380
AxyI CCTNAGG 1 cut(s) 811
BamHI GGATCC 2 cut(s) 29, 815
BanI GGYRCC 2 cut(s) 163, 235
BccI CCATC 6 cut(s) 79, 432, 485, 533, 631, 697
BciT130I CCWGG 1 cut(s) 176
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 2 cut(s) 149, 705
BfrI CTTAAG 1 cut(s) 91
BglI GCCNNNNNGGC 1 cut(s) 694
BisI GCNGC 2 cut(s) 801, 804
BlsI GCNGC 2 cut(s) 802, 805
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 2 cut(s) 176, 193
BmgT120I GGNCC 1 cut(s) 686
BmiI GGNNCC 5 cut(s) 31, 165, 237, 745, 817
BmrFI CCNGG 2 cut(s) 176, 193
BpuEI CTTGAG 2 cut(s) 377, 805
BpuMI CCSGG 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 806
BsaJI CCNNGG 2 cut(s) 159, 174
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 232, 753
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 4 cut(s) 167, 239, 374, 582
Bse21I CCTNAGG 1 cut(s) 811
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 2 cut(s) 159, 174
BseLI CCNNNNNNNGG 3 cut(s) 154, 232, 753
BseMII CTCAG 2 cut(s) 537, 825
BseNI ACTGG 4 cut(s) 167, 239, 374, 582
BseRI GAGGAG 1 cut(s) 215
BshFI GGCC 4 cut(s) 158, 248, 282, 688
BshNI GGYRCC 2 cut(s) 163, 235
BsiSI CCGG 2 cut(s) 155, 193
BslI CCNNNNNNNGG 3 cut(s) 154, 232, 753
BsmAI GTCTC 1 cut(s) 220
BsnI GGCC 4 cut(s) 158, 248, 282, 688
Bsp143I GATC 3 cut(s) 29, 775, 815
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 2 cut(s) 800, 803
BspANI GGCC 4 cut(s) 158, 248, 282, 688
BspCNI CTCAG 2 cut(s) 538, 824
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 5 cut(s) 31, 165, 237, 745, 817
BspPI GGATC 4 cut(s) 24, 37, 810, 823
BspT107I GGYRCC 2 cut(s) 163, 235
BspTI CTTAAG 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 4 cut(s) 167, 239, 374, 582
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 159, 174
BssMI GATC 3 cut(s) 29, 775, 815
BssNI GRCGYC 1 cut(s) 806
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 796
BstACI GRCGYC 1 cut(s) 806
BstAFI CTTAAG 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 156, 250, 473, 477
BstDEI CTNAG 4 cut(s) 8, 546, 611, 811
BstDSI CCRYGG 1 cut(s) 159
BstKTI GATC 3 cut(s) 32, 778, 818
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 3 cut(s) 29, 775, 815
BstMWI GCNNNNNNNGC 3 cut(s) 205, 472, 694
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 2 cut(s) 174, 191
BstX2I RGATCY 2 cut(s) 29, 815
BstYI RGATCY 2 cut(s) 29, 815
Bsu36I CCTNAGG 1 cut(s) 811
BsuRI GGCC 4 cut(s) 158, 248, 282, 688
BtgI CCRYGG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 5, 589
Cac8I GCNNGC 4 cut(s) 156, 250, 473, 477
CciI TCATGA 1 cut(s) 334
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 686
CseI GACGC 1 cut(s) 795
Csp6I GTAC 4 cut(s) 164, 236, 782, 825
CspCI CAANNNNNGTGG 4 cut(s) 298, 333, 777, 812
CviAII CATG 6 cut(s) 160, 335, 400, 452, 600, 680
CviQI GTAC 4 cut(s) 164, 236, 782, 825
DdeI CTNAG 4 cut(s) 8, 546, 611, 811
DpnI GATC 3 cut(s) 31, 777, 817
DpnII GATC 3 cut(s) 29, 775, 815
DraI TTTAAA 2 cut(s) 504, 724
EaeI YGGCCR 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 159
Eco81I CCTNAGG 1 cut(s) 811
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 6 cut(s) 163, 338, 403, 455, 603, 683
FalI AAGNNNNNCTT 4 cut(s) 74, 106, 351, 383
FatI CATG 6 cut(s) 159, 334, 399, 451, 599, 679
Fnu4HI GCNGC 2 cut(s) 801, 804
Fsp4HI GCNGC 2 cut(s) 801, 804
FspBI CTAG 2 cut(s) 149, 705
GluI GCNGC 2 cut(s) 801, 804
HaeIII GGCC 4 cut(s) 158, 248, 282, 688
HapII CCGG 2 cut(s) 155, 193
HgaI GACGC 1 cut(s) 795
Hin1I GRCGYC 1 cut(s) 806
Hin1II CATG 6 cut(s) 163, 338, 403, 455, 603, 683
HinfI GANTC 4 cut(s) 112, 123, 269, 548
HpaII CCGG 2 cut(s) 155, 193
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 1 cut(s) 662
Hpy188I TCNGA 5 cut(s) 294, 429, 517, 562, 673
Hpy188III TCNNGA 3 cut(s) 335, 406, 813
Hpy8I GTNNAC 1 cut(s) 662
Hpy99I CGWCG 1 cut(s) 318
HpyAV CCTTC 2 cut(s) 20, 754
HpyCH4III ACNGT 1 cut(s) 796
HpyCH4IV ACGT 2 cut(s) 313, 445
HpyCH4V TGCA 4 cut(s) 65, 208, 252, 455
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 472, 694
HpyF3I CTNAG 4 cut(s) 8, 546, 611, 811
HpySE526I ACGT 2 cut(s) 313, 445
Hsp92I GRCGYC 1 cut(s) 806
Hsp92II CATG 6 cut(s) 163, 338, 403, 455, 603, 683
KpnI GGTACC 2 cut(s) 167, 239
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 3 cut(s) 29, 775, 815
LmnI GCTCC 1 cut(s) 542
MaeI CTAG 2 cut(s) 149, 705
MaeII ACGT 2 cut(s) 313, 445
MaeIII GTNAC 1 cut(s) 266
MalI GATC 3 cut(s) 31, 777, 817
MboI GATC 3 cut(s) 29, 775, 815
MboII GAAGA 2 cut(s) 25, 94
MflI RGATCY 2 cut(s) 29, 815
MluCI AATT 9 cut(s) 17, 34, 48, 187, 259, 288, 496, 505, 717
MlyI GAGTC 2 cut(s) 263, 557
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 5 cut(s) 92, 186, 287, 503, 723
MspCI CTTAAG 1 cut(s) 91
MspI CCGG 2 cut(s) 155, 193
MspR9I CCNGG 2 cut(s) 176, 193
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 205, 472, 694
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 1 cut(s) 159
NdeII GATC 3 cut(s) 29, 775, 815
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 6 cut(s) 163, 338, 403, 455, 603, 683
NlaIV GGNNCC 5 cut(s) 31, 165, 237, 745, 817
NmuCI GTSAC 1 cut(s) 266
NspI RCATGY 1 cut(s) 403
PagI TCATGA 1 cut(s) 334
PciI ACATGT 1 cut(s) 399
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 112, 123
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 2 cut(s) 802, 805
PleI GAGTC 2 cut(s) 263, 556
PpsI GAGTC 2 cut(s) 263, 556
PscI ACATGT 1 cut(s) 399
PsiI TTATAA 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 5 cut(s) 31, 165, 237, 745, 817
PspPI GGNCC 1 cut(s) 686
PsrI GAACNNNNNNTAC 2 cut(s) 652, 684
PsuI RGATCY 2 cut(s) 29, 815
RsaI GTAC 4 cut(s) 165, 237, 783, 826
RsaNI GTAC 4 cut(s) 164, 236, 782, 825
SaqAI TTAA 5 cut(s) 92, 186, 287, 503, 723
SatI GCNGC 2 cut(s) 801, 804
Sau3AI GATC 3 cut(s) 29, 775, 815
Sau96I GGNCC 1 cut(s) 686
ScaI AGTACT 1 cut(s) 826
SchI GAGTC 2 cut(s) 263, 557
ScrFI CCNGG 2 cut(s) 176, 193
SmiI ATTTAAAT 1 cut(s) 504
SmlI CTYRAG 3 cut(s) 91, 392, 820
SmoI CTYRAG 3 cut(s) 91, 392, 820
Sse9I AATT 9 cut(s) 17, 34, 48, 187, 259, 288, 496, 505, 717
SsiI CCGC 2 cut(s) 800, 803
SspMI CTAG 2 cut(s) 149, 705
StyD4I CCNGG 2 cut(s) 174, 191
StyI CCWWGG 1 cut(s) 159
SwaI ATTTAAAT 1 cut(s) 504
TaaI ACNGT 1 cut(s) 796
TaiI ACGT 2 cut(s) 316, 448
TaqI TCGA 3 cut(s) 80, 328, 462
TasI AATT 9 cut(s) 17, 34, 48, 187, 259, 288, 496, 505, 717
TatI WGTACW 1 cut(s) 824
TauI GCSGC 2 cut(s) 803, 806
TfiI GAWTC 2 cut(s) 112, 123
Tru1I TTAA 5 cut(s) 92, 186, 287, 503, 723
Tru9I TTAA 5 cut(s) 92, 186, 287, 503, 723
TscAI CASTG 2 cut(s) 12, 589
TseFI GTSAC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 5 cut(s) 323, 351, 440, 579, 668
TspRI CASTG 2 cut(s) 12, 589
Vha464I CTTAAG 1 cut(s) 91
XapI RAATTY 1 cut(s) 717
XceI RCATGY 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 149, 705
ZrmI AGTACT 1 cut(s) 826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.