pycom12g16820

APO protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002143
Physical Location & Seq
Reverse (-)
19102689 .. 19103981
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16820.1

Sequence Viewer

Length: 951 bp
ATGGACAGCTTTGCAAGGTATTACAGCTTGAAGATGGACTTAAGGAAGCTAAAGCCTATGATTCAAAAGAGAATCCAAAACCGAGCTAAAGATTACCCAGTGCCGGCCATGGTACCAGTTGCCCAGGAGGTCCTTGATTCGCGGAGGCTCATTATGCAAGGTGTCTCTACTCTCCTCAAAATGGTACCAGTTTTGGCCTGCAAGTTCTGTCCAGAAGTATATATAGGTGAGAAAGGCCACTTAATTCAGACTTGTTGTGGCTTCAAACGTCGTGGCAAAAATCGAGTTCATGAATGGATAGTTGGTGGTTTGAAAGATGTGCTTGTTCCAGTAGAGGCATTTCACCTCAAGCACATGTTCCAGGATGTTATAAAACACCATCAGAGGTTTGACTTTGAACGTGTTCCTGCAGTTGTCGAGCTATGCTGGCAAGCAGGGGCAAATGATGGAAATTATTTAAATTGGAACTCTGAAAGTGATTGTGTTGGCGTTGATGGAGCTGAGTCTTTGTCATCTGATGAACTGATTCTGGTTGCCAGTGGAACTTTGAGGGCATGGGAAATACTTAGAAATGGCGTGGAGAAGTTGCTGATGGTTTATCCAGGGAAAGTCTGTAAACATTGTTCAGAAGTTCATGTTGGGCCATCTGGGCATAAGGCTAGGCTTTGCGGAATTTTTAAGTATGAGAGTTGGCAGGGAACCCATTTTTGGAGGAAGGCAAATGTAGATGATCTTGTACCTCCAAAGACTGTATGGCGGCGGCGTCCTCAGGATCCTCAAGTACTTTTGAATGAAGGAAGGGGATTTTATGGGCATGCTCCGGCAGTAGTAGAACTATGCACACAGGCAGGTGCCATTGCACCTCCAAAGTATCACTGCATGATGAAACTGCAGGGTGTAAGTACCATACTTGGAGATCAAAGGTTGCTCCACCCGAATGATCAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

317

Amino Acids

35.86

Weight (kDa)

9.05

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 11 - 148 1.6e-39 APO RNA-binding
APO_RNA-bind PF05634 172 - 300 1e-20 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AarI CACCTGC 1 cut(s) 839
Acc36I ACCTGC 1 cut(s) 839
Acc65I GGTACC 2 cut(s) 112, 184
AccB1I GGYRCC 3 cut(s) 112, 184, 851
AccII CGCG 1 cut(s) 142
AciI CCGC 4 cut(s) 142, 669, 757, 760
AclWI GGATC 2 cut(s) 767, 780
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 1 cut(s) 672
AcyI GRCGYC 1 cut(s) 763
AfaI GTAC 5 cut(s) 114, 186, 738, 783, 904
AfiI CCNNNNNNNGG 3 cut(s) 103, 181, 708
AflII CTTAAG 1 cut(s) 40
AflIII ACRYGT 2 cut(s) 354, 400
AgsI TTSAA 6 cut(s) 31, 65, 265, 313, 398, 790
AjnI CCWGG 3 cut(s) 123, 360, 601
AjuI GAANNNNNNNTTGG 6 cut(s) 285, 317, 621, 653, 691, 723
AluBI AGCT 6 cut(s) 9, 27, 49, 86, 421, 500
AluI AGCT 6 cut(s) 9, 27, 49, 86, 421, 500
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 2 cut(s) 767, 780
AoxI GGCC 4 cut(s) 105, 195, 235, 641
ApoI RAATTY 1 cut(s) 672
Asp700I GAANNNNTTC 2 cut(s) 402, 525
Asp718I GGTACC 2 cut(s) 112, 184
AspS9I GGNCC 2 cut(s) 130, 641
AsuHPI GGTGA 2 cut(s) 239, 335
AvaII GGWCC 1 cut(s) 130
AxyI CCTNAGG 1 cut(s) 768
BamHI GGATCC 1 cut(s) 772
BanI GGYRCC 3 cut(s) 112, 184, 851
BccI CCATC 6 cut(s) 28, 387, 440, 488, 586, 652
BciT130I CCWGG 3 cut(s) 125, 362, 603
BclI TGATCA 1 cut(s) 940
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 660
BfmI CTRYAG 2 cut(s) 408, 890
BfrI CTTAAG 1 cut(s) 40
BfuAI ACCTGC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 649
BisI GCNGC 2 cut(s) 758, 761
BlsI GCNGC 2 cut(s) 759, 762
BmcAI AGTACT 1 cut(s) 783
Bme1390I CCNGG 3 cut(s) 125, 362, 603
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 130, 641
BmiI GGNNCC 5 cut(s) 114, 186, 700, 774, 853
BmrFI CCNGG 3 cut(s) 125, 362, 603
BmrI ACTGGG 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 92
BpuEI CTTGAG 2 cut(s) 332, 762
BsaHI GRCGYC 1 cut(s) 763
BsaJI CCNNGG 3 cut(s) 108, 123, 602
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 181, 708
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 5 cut(s) 98, 116, 188, 329, 537
Bse21I CCTNAGG 1 cut(s) 768
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 3 cut(s) 125, 362, 603
BseDI CCNNGG 3 cut(s) 108, 123, 602
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 3 cut(s) 103, 181, 708
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 2 cut(s) 492, 782
BseNI ACTGG 5 cut(s) 98, 116, 188, 329, 537
BseRI GAGGAG 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 142
BshFI GGCC 4 cut(s) 107, 197, 237, 643
BshNI GGYRCC 3 cut(s) 112, 184, 851
BsiSI CCGG 2 cut(s) 104, 821
BslI CCNNNNNNNGG 3 cut(s) 103, 181, 708
BsmAI GTCTC 1 cut(s) 169
BsnI GGCC 4 cut(s) 107, 197, 237, 643
Bsp143I GATC 4 cut(s) 730, 772, 916, 940
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 4 cut(s) 142, 669, 757, 760
BspANI GGCC 4 cut(s) 107, 197, 237, 643
BspCNI CTCAG 2 cut(s) 493, 781
BspFNI CGCG 1 cut(s) 142
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 5 cut(s) 114, 186, 700, 774, 853
BspMAI CTGCAG 2 cut(s) 412, 894
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 2 cut(s) 767, 780
BspT107I GGYRCC 3 cut(s) 112, 184, 851
BspTI CTTAAG 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 5 cut(s) 98, 116, 188, 329, 537
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 3 cut(s) 108, 123, 602
BssMI GATC 4 cut(s) 730, 772, 916, 940
BssNI GRCGYC 1 cut(s) 763
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 3 cut(s) 125, 362, 603
Bst4CI ACNGT 1 cut(s) 751
BstACI GRCGYC 1 cut(s) 763
BstAFI CTTAAG 1 cut(s) 40
BstC8I GCNNGC 5 cut(s) 105, 199, 428, 432, 816
BstDEI CTNAG 3 cut(s) 501, 566, 768
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 370
BstFNI CGCG 1 cut(s) 142
BstKTI GATC 4 cut(s) 733, 775, 919, 943
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 4 cut(s) 730, 772, 916, 940
BstMWI GCNNNNNNNGC 3 cut(s) 154, 427, 649
BstNI CCWGG 3 cut(s) 125, 362, 603
BstNSI RCATGY 2 cut(s) 358, 818
BstSCI CCNGG 3 cut(s) 123, 360, 601
BstSFI CTRYAG 2 cut(s) 408, 890
BstUI CGCG 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
Bsu36I CCTNAGG 1 cut(s) 768
BsuRI GGCC 4 cut(s) 107, 197, 237, 643
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 874
BtsIMutI CAGTG 3 cut(s) 105, 544, 874
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 5 cut(s) 105, 199, 428, 432, 816
CciI TCATGA 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 130, 641
CseI GACGC 1 cut(s) 752
Csp6I GTAC 5 cut(s) 113, 185, 737, 782, 903
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 7 cut(s) 109, 290, 355, 555, 635, 815, 880
CviQI GTAC 5 cut(s) 113, 185, 737, 782, 903
DdeI CTNAG 3 cut(s) 501, 566, 768
DpnI GATC 4 cut(s) 732, 774, 918, 942
DpnII GATC 4 cut(s) 730, 772, 916, 940
DraI TTTAAA 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 130
Eco81I CCTNAGG 1 cut(s) 768
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 3 cut(s) 123, 360, 601
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 7 cut(s) 112, 293, 358, 558, 638, 818, 883
FalI AAGNNNNNCTT 4 cut(s) 23, 55, 306, 338
FatI CATG 7 cut(s) 108, 289, 354, 554, 634, 814, 879
FbaI TGATCA 1 cut(s) 940
Fnu4HI GCNGC 2 cut(s) 758, 761
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 2 cut(s) 758, 761
FspBI CTAG 1 cut(s) 660
GluI GCNGC 2 cut(s) 758, 761
HaeIII GGCC 4 cut(s) 107, 197, 237, 643
HapII CCGG 2 cut(s) 104, 821
HgaI GACGC 1 cut(s) 752
Hin1I GRCGYC 1 cut(s) 763
Hin1II CATG 7 cut(s) 112, 293, 358, 558, 638, 818, 883
HinfI GANTC 5 cut(s) 61, 72, 137, 503, 526
HpaII CCGG 2 cut(s) 104, 821
HphI GGTGA 2 cut(s) 239, 335
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 5 cut(s) 249, 384, 472, 517, 628
Hpy188III TCNNGA 3 cut(s) 212, 290, 770
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 3 cut(s) 709, 788, 792
HpyCH4III ACNGT 1 cut(s) 751
HpyCH4IV ACGT 2 cut(s) 268, 400
HpyCH4V TGCA 8 cut(s) 14, 157, 201, 410, 840, 860, 879, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 154, 427, 649
HpyF3I CTNAG 3 cut(s) 501, 566, 768
HpySE526I ACGT 2 cut(s) 268, 400
Hsp92I GRCGYC 1 cut(s) 763
Hsp92II CATG 7 cut(s) 112, 293, 358, 558, 638, 818, 883
KpnI GGTACC 2 cut(s) 116, 188
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 940
Kzo9I GATC 4 cut(s) 730, 772, 916, 940
LmnI GCTCC 3 cut(s) 497, 823, 933
MaeI CTAG 1 cut(s) 660
MaeII ACGT 2 cut(s) 268, 400
MalI GATC 4 cut(s) 732, 774, 918, 942
MboI GATC 4 cut(s) 730, 772, 916, 940
MboII GAAGA 1 cut(s) 43
MflI RGATCY 1 cut(s) 772
MluCI AATT 4 cut(s) 243, 451, 460, 672
MlyI GAGTC 1 cut(s) 512
MroNI GCCGGC 1 cut(s) 103
MroXI GAANNNNTTC 2 cut(s) 402, 525
MseI TTAA 4 cut(s) 41, 242, 458, 678
MslI CAYNNNNRTG 1 cut(s) 936
MspCI CTTAAG 1 cut(s) 40
MspI CCGG 2 cut(s) 104, 821
MspR9I CCNGG 3 cut(s) 125, 362, 603
MvaI CCWGG 3 cut(s) 125, 362, 603
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 3 cut(s) 154, 427, 649
NaeI GCCGGC 1 cut(s) 105
NcoI CCATGG 1 cut(s) 108
NdeII GATC 4 cut(s) 730, 772, 916, 940
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 7 cut(s) 112, 293, 358, 558, 638, 818, 883
NlaIV GGNNCC 5 cut(s) 114, 186, 700, 774, 853
NspI RCATGY 2 cut(s) 358, 818
PaeI GCATGC 1 cut(s) 818
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 839
PciI ACATGT 1 cut(s) 354
PdiI GCCGGC 1 cut(s) 105
PdmI GAANNNNTTC 2 cut(s) 402, 525
PfeI GAWTC 4 cut(s) 61, 72, 137, 526
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 2 cut(s) 759, 762
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PpuMI RGGWCCY 1 cut(s) 130
PscI ACATGT 1 cut(s) 354
PsiI TTATAA 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 3 cut(s) 123, 360, 601
PspGI CCWGG 3 cut(s) 123, 360, 601
PspN4I GGNNCC 5 cut(s) 114, 186, 700, 774, 853
PspPI GGNCC 2 cut(s) 130, 641
PspPPI RGGWCCY 1 cut(s) 130
PsrI GAACNNNNNNTAC 2 cut(s) 607, 639
PstI CTGCAG 2 cut(s) 412, 894
PsuI RGATCY 1 cut(s) 772
RsaI GTAC 5 cut(s) 114, 186, 738, 783, 904
RsaNI GTAC 5 cut(s) 113, 185, 737, 782, 903
RseI CAYNNNNRTG 1 cut(s) 936
SaqAI TTAA 4 cut(s) 41, 242, 458, 678
SatI GCNGC 2 cut(s) 758, 761
Sau3AI GATC 4 cut(s) 730, 772, 916, 940
Sau96I GGNCC 2 cut(s) 130, 641
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 3 cut(s) 125, 362, 603
SfcI CTRYAG 2 cut(s) 408, 890
SinI GGWCC 1 cut(s) 130
SmiI ATTTAAAT 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 936
SmlI CTYRAG 3 cut(s) 40, 347, 777
SmoI CTYRAG 3 cut(s) 40, 347, 777
SphI GCATGC 1 cut(s) 818
Sse9I AATT 4 cut(s) 243, 451, 460, 672
SsiI CCGC 4 cut(s) 142, 669, 757, 760
SspMI CTAG 1 cut(s) 660
StyD4I CCNGG 3 cut(s) 123, 360, 601
StyI CCWWGG 1 cut(s) 108
SwaI ATTTAAAT 1 cut(s) 459
TaaI ACNGT 1 cut(s) 751
TaiI ACGT 2 cut(s) 271, 403
TaqI TCGA 2 cut(s) 283, 417
TasI AATT 4 cut(s) 243, 451, 460, 672
TatI WGTACW 1 cut(s) 781
TauI GCSGC 2 cut(s) 760, 763
TfiI GAWTC 4 cut(s) 61, 72, 137, 526
Tru1I TTAA 4 cut(s) 41, 242, 458, 678
Tru9I TTAA 4 cut(s) 41, 242, 458, 678
TscAI CASTG 3 cut(s) 105, 544, 881
TspDTI ATGAA 6 cut(s) 278, 306, 534, 623, 807, 899
TspRI CASTG 3 cut(s) 105, 544, 881
Vha464I CTTAAG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 130
XapI RAATTY 1 cut(s) 672
XceI RCATGY 2 cut(s) 358, 818
XcmI CCANNNNNNNNNTGG 1 cut(s) 750
XmnI GAANNNNTTC 2 cut(s) 402, 525
XspI CTAG 1 cut(s) 660
ZrmI AGTACT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.