pycom09g02880

APO protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002143
Physical Location & Seq
Reverse (-)
2090599 .. 2092726
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02880.1

Sequence Viewer

Length: 1107 bp
ATGGCACTGAGGAAGAAATTGTGCGAGAGATCCAATTTCAATGGCAAAATTATGGACTGCTTTGCAAGGTATTACAGCTCAAAGATGGGCTTAAGGAAGCTAAAGCCTATGATTCAAAAGAGAATCCAAAACCGAGCTAAAGATTACCCAGTGCCGGCCATGGTACCAGTTGCACAGGAGGTCCTTGATTCCGGGAGGCTTGTTATGCAAGGTGTCTCTACTCTCCTCAAAATGGATGTGCTTGTTCCAGTAAAGGTATTTCACCTCAAGCACATGTTCCAGGATGTTATAAAACACCATCAGAGGTTTGACTTTGAATGTGTTCCTGCAGTTGTTGAGCTATGCTGGCAAGCAGGGGCAAATGATGGAAATCATTTAAATTGGAACTCTGAAAGTGATTGTGTTGGCGTTGATGGAGTTGAGTCTTTGTCATCTGATGAACTGATTCTGGTTGCCAGTGGAACTTTGAGGGCATGGGAAATACTTAGAAATGGCGTGGAGAAGTTGCTGATGGTTTATCCAGCGAAAGTCTGTAAACATTGTTCAGAAGTTCATGTTGGGCCATCTGGGCATAAGGCTAGGCTTTGCGGAATTTTTAAGTATGAGAGTTGGCAGGGAACCCATTTTTGGAGGAAGGCAAATGTAGATGATCTTGTACCTCCAAAGACTCTACAGCGCCGGCATCCTCAGGATCCTCAAGTACTTTTGAATGAAGGGAGGGGATATTATGGGCATGCTCCGGCAGTAGTAGAACCATGCACACGGGCAAGTGCCATTGCACCTCCAAAGTATCACTGCATGATGATACTGCAGGGTACTGTCGACAGATGCCGCTTGAAGCTGAAGTTTCCTGCTCAGAAGCAATTGCCTCTGCTCATTGACTGTGCTCGAGTCTTCAGTTTCACCATAATGCTCGTCACATCGAGCGAACTCGAGGCAGTTTGTACCACAAGACTCAATCTCCTCGACGGAGGGTTGCTCTTTATTCCATTCATGATCTTGAGGAACAAAAGGATCTCGGAAAATTCACAAATGCCGTACTTGCTCAAGTTGCGAAATTCGTTTTCCAACTCGATTCCACAGACAAAAGAGTTGACATTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

369

Amino Acids

41.83

Weight (kDa)

9.33

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 79 - 120 4.3e-10 APO RNA-binding
APO_RNA-bind PF05634 145 - 257 1.1e-17 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 163
AccI GTMKAC 1 cut(s) 822
AciI CCGC 2 cut(s) 588, 832
AclWI GGATC 4 cut(s) 24, 686, 699, 1022
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 3 cut(s) 591, 1024, 1057
AcuI CTGAAG 2 cut(s) 863, 880
AfaI GTAC 6 cut(s) 165, 657, 702, 817, 946, 1040
AfiI CCNNNNNNNGG 3 cut(s) 154, 232, 627
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 5 cut(s) 40, 116, 317, 709, 838
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 4 cut(s) 540, 572, 610, 642
AluBI AGCT 5 cut(s) 78, 100, 137, 340, 841
AluI AGCT 5 cut(s) 78, 100, 137, 340, 841
Alw21I GWGCWC 1 cut(s) 889
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 4 cut(s) 24, 686, 699, 1022
Ama87I CYCGRG 2 cut(s) 888, 932
AoxI GGCC 2 cut(s) 156, 560
ApoI RAATTY 3 cut(s) 591, 1024, 1057
Asp700I GAANNNNTTC 2 cut(s) 321, 444
Asp718I GGTACC 1 cut(s) 163
AspLEI GCGC 1 cut(s) 678
AspS9I GGNCC 2 cut(s) 181, 560
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 2 cut(s) 254, 895
AvaI CYCGRG 2 cut(s) 888, 932
AvaII GGWCC 1 cut(s) 181
AxyI CCTNAGG 1 cut(s) 687
BamHI GGATCC 1 cut(s) 691
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 886
Bbv12I GWGCWC 1 cut(s) 889
BccI CCATC 6 cut(s) 79, 306, 359, 407, 505, 571
BceAI ACGGC 1 cut(s) 1021
BciT130I CCWGG 1 cut(s) 281
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 579
BfmI CTRYAG 3 cut(s) 327, 671, 809
BfoI RGCGCY 1 cut(s) 679
BfrI CTTAAG 1 cut(s) 91
BglI GCCNNNNNGGC 1 cut(s) 568
BisI GCNGC 1 cut(s) 832
BlsI GCNGC 1 cut(s) 833
BmcAI AGTACT 1 cut(s) 702
Bme1390I CCNGG 2 cut(s) 193, 281
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 2 cut(s) 888, 932
BmgT120I GGNCC 2 cut(s) 181, 560
BmiI GGNNCC 3 cut(s) 165, 619, 693
BmrFI CCNGG 2 cut(s) 193, 281
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 691, 818
BmuI ACTGGG 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 886
BplI GAGNNNNNCTC 2 cut(s) 963, 995
BpuEI CTTGAG 4 cut(s) 251, 681, 1021, 1031
BpuMI CCSGG 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 945, 975
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 232, 627
Bse118I RCCGGY 2 cut(s) 154, 678
Bse1I ACTGG 4 cut(s) 149, 167, 248, 456
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 1 cut(s) 774
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 3 cut(s) 241, 289, 682
BseJI GATNNNNATC 1 cut(s) 369
BseLI CCNNNNNNNGG 3 cut(s) 154, 232, 627
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 2 cut(s) 701, 869
BseNI ACTGG 4 cut(s) 149, 167, 248, 456
BseRI GAGGAG 2 cut(s) 215, 953
BshFI GGCC 2 cut(s) 158, 562
BshNI GGYRCC 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 889
BsiHKCI CYCGRG 2 cut(s) 888, 932
BsiSI CCGG 4 cut(s) 155, 192, 679, 740
BslI CCNNNNNNNGG 3 cut(s) 154, 232, 627
BsmAI GTCTC 1 cut(s) 220
BsnI GGCC 2 cut(s) 158, 562
BsoBI CYCGRG 2 cut(s) 888, 932
Bsp1286I GDGCHC 1 cut(s) 889
Bsp143I GATC 5 cut(s) 29, 649, 691, 996, 1014
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 2 cut(s) 588, 832
BspANI GGCC 2 cut(s) 158, 562
BspCNI CTCAG 2 cut(s) 700, 868
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 3 cut(s) 165, 619, 693
BspMAI CTGCAG 2 cut(s) 331, 813
BspPI GGATC 4 cut(s) 24, 686, 699, 1022
BspT107I GGYRCC 1 cut(s) 163
BspTI CTTAAG 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 2 cut(s) 154, 678
BsrI ACTGG 4 cut(s) 149, 167, 248, 456
BssAI RCCGGY 2 cut(s) 154, 678
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 5 cut(s) 29, 649, 691, 996, 1014
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 2 cut(s) 820, 884
BstAFI CTTAAG 1 cut(s) 91
BstC8I GCNNGC 5 cut(s) 156, 347, 351, 680, 735
BstDEI CTNAG 4 cut(s) 8, 485, 687, 855
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 3 cut(s) 241, 289, 682
BstH2I RGCGCY 1 cut(s) 679
BstHHI GCGC 1 cut(s) 678
BstKTI GATC 5 cut(s) 32, 652, 694, 999, 1017
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 5 cut(s) 29, 649, 691, 996, 1014
BstMWI GCNNNNNNNGC 5 cut(s) 205, 346, 568, 1042, 1051
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 2 cut(s) 277, 737
BstSCI CCNGG 2 cut(s) 191, 279
BstSFI CTRYAG 3 cut(s) 327, 671, 809
BstV2I GAAGAC 1 cut(s) 886
BstX2I RGATCY 3 cut(s) 29, 691, 1014
BstYI RGATCY 3 cut(s) 29, 691, 1014
Bsu36I CCTNAGG 1 cut(s) 687
BsuRI GGCC 2 cut(s) 158, 562
BtgI CCRYGG 1 cut(s) 159
BtsCI GGATG 3 cut(s) 241, 289, 682
BtsI GCAGTG 1 cut(s) 793
BtsIMutI CAGTG 4 cut(s) 5, 156, 463, 793
Cac8I GCNNGC 5 cut(s) 156, 347, 351, 680, 735
CciI TCATGA 1 cut(s) 993
CfoI GCGC 1 cut(s) 678
Cfr10I RCCGGY 2 cut(s) 154, 678
Cfr13I GGNCC 2 cut(s) 181, 560
Csp6I GTAC 6 cut(s) 164, 656, 701, 816, 945, 1039
CviAII CATG 8 cut(s) 160, 274, 474, 554, 734, 756, 799, 994
CviQI GTAC 6 cut(s) 164, 656, 701, 816, 945, 1039
DdeI CTNAG 4 cut(s) 8, 485, 687, 855
DpnI GATC 5 cut(s) 31, 651, 693, 998, 1016
DpnII GATC 5 cut(s) 29, 649, 691, 996, 1014
DraI TTTAAA 1 cut(s) 378
EaeI YGGCCR 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 863, 880
Eco81I CCTNAGG 1 cut(s) 687
Eco88I CYCGRG 2 cut(s) 888, 932
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 8 cut(s) 163, 277, 477, 557, 737, 759, 802, 997
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FatI CATG 8 cut(s) 159, 273, 473, 553, 733, 755, 798, 993
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 1 cut(s) 832
FokI GGATG 3 cut(s) 248, 296, 669
Fsp4HI GCNGC 1 cut(s) 832
FspBI CTAG 1 cut(s) 579
GlaI GCGC 1 cut(s) 677
GluI GCNGC 1 cut(s) 832
HaeII RGCGCY 1 cut(s) 679
HaeIII GGCC 2 cut(s) 158, 562
HapII CCGG 4 cut(s) 155, 192, 679, 740
HhaI GCGC 1 cut(s) 678
Hin1II CATG 8 cut(s) 163, 277, 477, 557, 737, 759, 802, 997
Hin6I GCGC 1 cut(s) 676
HinP1I GCGC 1 cut(s) 676
HincII GTYRAC 2 cut(s) 823, 1095
HindII GTYRAC 2 cut(s) 823, 1095
HinfI GANTC 9 cut(s) 112, 123, 188, 422, 445, 667, 891, 954, 1075
HpaII CCGG 4 cut(s) 155, 192, 679, 740
HphI GGTGA 2 cut(s) 254, 895
Hpy166II GTNNAC 3 cut(s) 536, 823, 1095
Hpy188I TCNGA 6 cut(s) 303, 391, 436, 547, 858, 1021
Hpy188III TCNNGA 3 cut(s) 689, 994, 1000
Hpy8I GTNNAC 3 cut(s) 536, 823, 1095
Hpy99I CGWCG 1 cut(s) 971
HpyAV CCTTC 2 cut(s) 628, 707
HpyCH4III ACNGT 2 cut(s) 820, 884
HpyCH4V TGCA 8 cut(s) 65, 173, 208, 329, 759, 779, 798, 811
HpyF10VI GCNNNNNNNGC 5 cut(s) 205, 346, 568, 1042, 1051
HpyF3I CTNAG 4 cut(s) 8, 485, 687, 855
Hsp92II CATG 8 cut(s) 163, 277, 477, 557, 737, 759, 802, 997
HspAI GCGC 1 cut(s) 676
KpnI GGTACC 1 cut(s) 167
KroI GCCGGC 2 cut(s) 154, 678
KroNI GCCGGC 2 cut(s) 156, 680
Kzo9I GATC 5 cut(s) 29, 649, 691, 996, 1014
LmnI GCTCC 1 cut(s) 742
LweI GCATC 2 cut(s) 691, 818
MaeI CTAG 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 916
MalI GATC 5 cut(s) 31, 651, 693, 998, 1016
MboI GATC 5 cut(s) 29, 649, 691, 996, 1014
MboII GAAGA 2 cut(s) 25, 886
MfeI CAATTG 1 cut(s) 863
MflI RGATCY 3 cut(s) 29, 691, 1014
MhlI GDGCHC 1 cut(s) 889
MluCI AATT 8 cut(s) 17, 34, 48, 379, 591, 863, 1024, 1057
MlyI GAGTC 4 cut(s) 431, 661, 900, 948
MmeI TCCRAC 1 cut(s) 1092
MroNI GCCGGC 2 cut(s) 154, 678
MroXI GAANNNNTTC 2 cut(s) 321, 444
MseI TTAA 3 cut(s) 92, 377, 597
MslI CAYNNNNRTG 1 cut(s) 908
MspCI CTTAAG 1 cut(s) 91
MspI CCGG 4 cut(s) 155, 192, 679, 740
MspR9I CCNGG 2 cut(s) 193, 281
MunI CAATTG 1 cut(s) 863
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 5 cut(s) 205, 346, 568, 1042, 1051
NaeI GCCGGC 2 cut(s) 156, 680
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 1 cut(s) 159
NdeII GATC 5 cut(s) 29, 649, 691, 996, 1014
NgoMIV GCCGGC 2 cut(s) 154, 678
NlaIII CATG 8 cut(s) 163, 277, 477, 557, 737, 759, 802, 997
NlaIV GGNNCC 3 cut(s) 165, 619, 693
NmuCI GTSAC 1 cut(s) 916
NspI RCATGY 2 cut(s) 277, 737
PaeI GCATGC 1 cut(s) 737
PaeR7I CTCGAG 2 cut(s) 888, 932
PagI TCATGA 1 cut(s) 993
PciI ACATGT 1 cut(s) 273
PdiI GCCGGC 2 cut(s) 156, 680
PdmI GAANNNNTTC 2 cut(s) 321, 444
PfeI GAWTC 5 cut(s) 112, 123, 188, 445, 1075
PfoI TCCNGGA 2 cut(s) 191, 279
PkrI GCNGC 1 cut(s) 833
PleI GAGTC 4 cut(s) 430, 661, 899, 948
PpsI GAGTC 4 cut(s) 430, 661, 899, 948
PpuMI RGGWCCY 1 cut(s) 181
PscI ACATGT 1 cut(s) 273
PsiI TTATAA 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 3 cut(s) 165, 619, 693
PspPI GGNCC 2 cut(s) 181, 560
PspPPI RGGWCCY 1 cut(s) 181
PspXI VCTCGAGB 2 cut(s) 888, 932
PsrI GAACNNNNNNTAC 2 cut(s) 526, 558
PstI CTGCAG 2 cut(s) 331, 813
PsuI RGATCY 3 cut(s) 29, 691, 1014
RsaI GTAC 6 cut(s) 165, 657, 702, 817, 946, 1040
RsaNI GTAC 6 cut(s) 164, 656, 701, 816, 945, 1039
RseI CAYNNNNRTG 1 cut(s) 908
SalI GTCGAC 1 cut(s) 821
SaqAI TTAA 3 cut(s) 92, 377, 597
SatI GCNGC 1 cut(s) 832
Sau3AI GATC 5 cut(s) 29, 649, 691, 996, 1014
Sau96I GGNCC 2 cut(s) 181, 560
ScaI AGTACT 1 cut(s) 702
SchI GAGTC 4 cut(s) 431, 661, 900, 948
ScrFI CCNGG 2 cut(s) 193, 281
SduI GDGCHC 1 cut(s) 889
SfaNI GCATC 2 cut(s) 691, 818
SfcI CTRYAG 3 cut(s) 327, 671, 809
Sfr274I CTCGAG 2 cut(s) 888, 932
SinI GGWCC 1 cut(s) 181
SlaI CTCGAG 2 cut(s) 888, 932
SmiI ATTTAAAT 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 908
SmlI CTYRAG 7 cut(s) 91, 266, 696, 888, 932, 1000, 1046
SmoI CTYRAG 7 cut(s) 91, 266, 696, 888, 932, 1000, 1046
SphI GCATGC 1 cut(s) 737
Sse9I AATT 8 cut(s) 17, 34, 48, 379, 591, 863, 1024, 1057
SsiI CCGC 2 cut(s) 588, 832
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 191, 279
StyI CCWWGG 1 cut(s) 159
SwaI ATTTAAAT 1 cut(s) 378
TaaI ACNGT 2 cut(s) 820, 884
TaqI TCGA 6 cut(s) 822, 889, 923, 933, 966, 1073
TasI AATT 8 cut(s) 17, 34, 48, 379, 591, 863, 1024, 1057
TatI WGTACW 1 cut(s) 700
TauI GCSGC 1 cut(s) 834
TfiI GAWTC 5 cut(s) 112, 123, 188, 445, 1075
Tru1I TTAA 3 cut(s) 92, 377, 597
Tru9I TTAA 3 cut(s) 92, 377, 597
TscAI CASTG 4 cut(s) 12, 156, 463, 800
TseFI GTSAC 1 cut(s) 916
Tsp45I GTSAC 1 cut(s) 916
TspDTI ATGAA 4 cut(s) 453, 542, 726, 982
TspGWI ACGGA 1 cut(s) 984
TspRI CASTG 4 cut(s) 12, 156, 463, 800
Vha464I CTTAAG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 3 cut(s) 591, 1024, 1057
XceI RCATGY 2 cut(s) 277, 737
XhoI CTCGAG 2 cut(s) 888, 932
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 2 cut(s) 321, 444
XspI CTAG 1 cut(s) 579
ZrmI AGTACT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.