pycom09g04290

APO protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002143
Physical Location & Seq
Forward (+)
3127379 .. 3129425
2047 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04290.2

Sequence Viewer

Length: 1098 bp
ATGGCATTGAGGAAGAAATTGTGCGATCGATCCAATTTCAATGGTAAAATTATGGACAGCTTTGCAAGGTATTACAGCTCGAAGATGGACTTAAGGAAGCTGAAGCCTATGATTCAAAAGAGAATCCAAAACCGAGCTAAAGATTACCCAGTGCCGGCCATGTTACCAGTTGCCCAGGAGGTCCTTGATTCCCGGAGGCTCGTTATGCAAGGTGTCTCTACTCTCCTCAAAATGGTACCAGTTTTGGCCTGCAAGTTCTGTCCAGAAGTATATATAGGTGAGAAAGGCCACTTAATTCAAACTTGTTGTGGCTTCAAACGTCGTGGCAAAAATCGAGTTCATGAGTGGATAGTTGGTGGTTTGAAAGATGTGCTTGTTCCAGTAGAGGCATTTCACCTCAAGCACATGTTCCAGGATGTTATAAAACACCATCAGAGGTTTGACTTTGAACGTGTTCCTGCAGTTGTCGAGCTATGCTGGCAAGCAGGGGCAAATGATGGAAATCATTTAAATTGGAACTCTGAAAGTGATTGTGTTAGCGTTGATGGAGCTGAGTCTTTGTCATCTGATGAACTGATTCTGGTTGCCAGTGGAACTTTAAGGGCATGGGAAATACTTAGAAATGGCGTGGAGAAGTTGCTGATGGTTTATCCAGCGAAAGTCTGTAAACATTGTTCAGAAGTTCATGTTGGGCCATCTGGGCATAAGGCTAGGCTTTGCGGAATTTTTAAGTATGAGAGTTGGCAGGGAACCCATTTTTGGAGGAAGGCAAATGTAGATGATCTTGTACCTCCAAAGACTGTATGGCGGCGGCGTCCTCAGGATCCTCAAGTACTTTTGAATGAAGGGAGGGGATTTTATGGGCATGCTCCGGCAGTAGTAGAACTATGCACGCAGGCAGGTGCCATTGCACCTCCAAAGTATCACTGCATGATGAAACTGCAGGGTGTAAGTACCATACTTGGAGATCAAAGTTTCACCAGAATGCTGGTCGCATCGAGCAAACTCGAGGCAGTTTGTGCCACAAGACCAGGGCTGGCCTTGAGAATTCGTGGGGCCATAGGCGAGCTTTTGAAGTGGGGTCGTAGAGTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

366

Amino Acids

41.2

Weight (kDa)

9.53

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
AarI CACCTGC 1 cut(s) 890
Acc36I ACCTGC 1 cut(s) 890
Acc65I GGTACC 1 cut(s) 235
AccB1I GGYRCC 2 cut(s) 235, 902
AciI CCGC 3 cut(s) 720, 808, 811
AclWI GGATC 3 cut(s) 24, 818, 831
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 723, 1047
AcuI CTGAAG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 814
AfaI GTAC 4 cut(s) 237, 789, 834, 955
AfiI CCNNNNNNNGG 3 cut(s) 154, 232, 759
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 2 cut(s) 405, 451
AgsI TTSAA 8 cut(s) 40, 116, 299, 316, 364, 449, 841, 1075
AjnI CCWGG 3 cut(s) 174, 411, 1030
AjuI GAANNNNNNNTTGG 4 cut(s) 672, 704, 742, 774
AluBI AGCT 7 cut(s) 60, 78, 100, 137, 472, 551, 1069
AluI AGCT 7 cut(s) 60, 78, 100, 137, 472, 551, 1069
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 3 cut(s) 24, 818, 831
Ama87I CYCGRG 1 cut(s) 1007
AoxI GGCC 6 cut(s) 156, 246, 286, 692, 1038, 1056
ApoI RAATTY 2 cut(s) 723, 1047
Asp700I GAANNNNTTC 2 cut(s) 453, 576
Asp718I GGTACC 1 cut(s) 235
AspS9I GGNCC 3 cut(s) 181, 692, 1056
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 3 cut(s) 290, 386, 970
AvaI CYCGRG 1 cut(s) 1007
AvaII GGWCC 1 cut(s) 181
AxyI CCTNAGG 1 cut(s) 819
BamHI GGATCC 1 cut(s) 823
BanI GGYRCC 2 cut(s) 235, 902
BccI CCATC 6 cut(s) 79, 438, 491, 539, 637, 703
BciT130I CCWGG 3 cut(s) 176, 413, 1032
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 220
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 459, 941
BfrI CTTAAG 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 890
BglI GCCNNNNNGGC 1 cut(s) 700
BisI GCNGC 2 cut(s) 809, 812
BlsI GCNGC 2 cut(s) 810, 813
BmcAI AGTACT 1 cut(s) 834
Bme1390I CCNGG 4 cut(s) 176, 193, 413, 1032
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 1007
BmgT120I GGNCC 3 cut(s) 181, 692, 1056
BmiI GGNNCC 5 cut(s) 237, 751, 825, 904, 1057
BmrFI CCNGG 4 cut(s) 176, 193, 413, 1032
BmrI ACTGGG 1 cut(s) 143
BmsI GCATC 1 cut(s) 1004
BmuI ACTGGG 1 cut(s) 143
BpuEI CTTGAG 3 cut(s) 383, 813, 1063
BpuMI CCSGG 1 cut(s) 193
Bsa29I ATCGAT 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 814
BsaJI CCNNGG 2 cut(s) 174, 1031
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 232, 759
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 5 cut(s) 149, 167, 239, 380, 588
Bse21I CCTNAGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 906
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 3 cut(s) 176, 413, 1032
BseCI ATCGAT 1 cut(s) 28
BseDI CCNNGG 2 cut(s) 174, 1031
BseGI GGATG 1 cut(s) 421
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 3 cut(s) 154, 232, 759
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 2 cut(s) 543, 833
BseNI ACTGG 5 cut(s) 149, 167, 239, 380, 588
BseRI GAGGAG 1 cut(s) 215
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 6 cut(s) 158, 248, 288, 694, 1040, 1058
BshNI GGYRCC 2 cut(s) 235, 902
BshVI ATCGAT 1 cut(s) 28
BsiEI CGRYCG 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 1007
BsiSI CCGG 3 cut(s) 155, 193, 872
BslI CCNNNNNNNGG 3 cut(s) 154, 232, 759
BsmAI GTCTC 1 cut(s) 220
BsmI GAATGC 1 cut(s) 990
BsnI GGCC 6 cut(s) 158, 248, 288, 694, 1040, 1058
BsoBI CYCGRG 1 cut(s) 1007
Bsp143I GATC 5 cut(s) 25, 29, 781, 823, 967
BspACI CCGC 3 cut(s) 720, 808, 811
BspANI GGCC 6 cut(s) 158, 248, 288, 694, 1040, 1058
BspCNI CTCAG 2 cut(s) 544, 832
BspDI ATCGAT 1 cut(s) 28
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 5 cut(s) 237, 751, 825, 904, 1057
BspMAI CTGCAG 2 cut(s) 463, 945
BspMI ACCTGC 1 cut(s) 890
BspPI GGATC 3 cut(s) 24, 818, 831
BspT107I GGYRCC 2 cut(s) 235, 902
BspTI CTTAAG 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 906
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 5 cut(s) 149, 167, 239, 380, 588
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 174, 1031
BssMI GATC 5 cut(s) 25, 29, 781, 823, 967
BssNI GRCGYC 1 cut(s) 814
Bst2UI CCWGG 3 cut(s) 176, 413, 1032
Bst4CI ACNGT 1 cut(s) 802
BstACI GRCGYC 1 cut(s) 814
BstAFI CTTAAG 1 cut(s) 91
BstAPI GCANNNNNTGC 1 cut(s) 1019
BstC8I GCNNGC 9 cut(s) 156, 250, 479, 483, 867, 893, 897, 1038, 1067
BstDEI CTNAG 3 cut(s) 552, 617, 819
BstF5I GGATG 1 cut(s) 421
BstKTI GATC 5 cut(s) 28, 32, 784, 826, 970
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 5 cut(s) 25, 29, 781, 823, 967
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 4 cut(s) 205, 478, 700, 1019
BstNI CCWGG 3 cut(s) 176, 413, 1032
BstNSI RCATGY 2 cut(s) 409, 869
BstSCI CCNGG 4 cut(s) 174, 191, 411, 1030
BstSFI CTRYAG 2 cut(s) 459, 941
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 1 cut(s) 988
BstYI RGATCY 1 cut(s) 823
Bsu15I ATCGAT 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 819
BsuRI GGCC 6 cut(s) 158, 248, 288, 694, 1040, 1058
BsuTUI ATCGAT 1 cut(s) 28
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 3 cut(s) 156, 595, 925
BveI ACCTGC 1 cut(s) 890
Cac8I GCNNGC 9 cut(s) 156, 250, 479, 483, 867, 893, 897, 1038, 1067
CciI TCATGA 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 181, 692, 1056
ClaI ATCGAT 1 cut(s) 28
CseI GACGC 1 cut(s) 803
Csp6I GTAC 4 cut(s) 236, 788, 833, 954
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 7 cut(s) 160, 341, 406, 606, 686, 866, 931
CviQI GTAC 4 cut(s) 236, 788, 833, 954
DdeI CTNAG 3 cut(s) 552, 617, 819
DpnI GATC 5 cut(s) 27, 31, 783, 825, 969
DpnII GATC 5 cut(s) 25, 29, 781, 823, 967
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 122
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 1007
EcoO109I RGGNCCY 1 cut(s) 181
EcoRI GAATTC 1 cut(s) 1047
EcoRII CCWGG 3 cut(s) 174, 411, 1030
FaeI CATG 7 cut(s) 163, 344, 409, 609, 689, 869, 934
FalI AAGNNNNNCTT 4 cut(s) 74, 106, 357, 389
FatI CATG 7 cut(s) 159, 340, 405, 605, 685, 865, 930
Fnu4HI GCNGC 2 cut(s) 809, 812
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 2 cut(s) 809, 812
FspBI CTAG 1 cut(s) 711
GluI GCNGC 2 cut(s) 809, 812
HaeIII GGCC 6 cut(s) 158, 248, 288, 694, 1040, 1058
HapII CCGG 3 cut(s) 155, 193, 872
HgaI GACGC 1 cut(s) 803
Hin1I GRCGYC 1 cut(s) 814
Hin1II CATG 7 cut(s) 163, 344, 409, 609, 689, 869, 934
HinfI GANTC 5 cut(s) 112, 123, 188, 554, 577
HpaII CCGG 3 cut(s) 155, 193, 872
HphI GGTGA 3 cut(s) 290, 386, 970
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 5 cut(s) 435, 523, 568, 679, 1097
Hpy188III TCNNGA 3 cut(s) 263, 341, 821
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 1 cut(s) 324
HpyAV CCTTC 2 cut(s) 760, 839
HpyCH4III ACNGT 1 cut(s) 802
HpyCH4IV ACGT 2 cut(s) 319, 451
HpyCH4V TGCA 8 cut(s) 65, 208, 252, 461, 891, 911, 930, 943
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 478, 700, 1019
HpyF3I CTNAG 3 cut(s) 552, 617, 819
HpySE526I ACGT 2 cut(s) 319, 451
Hsp92I GRCGYC 1 cut(s) 814
Hsp92II CATG 7 cut(s) 163, 344, 409, 609, 689, 869, 934
KpnI GGTACC 1 cut(s) 239
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 5 cut(s) 25, 29, 781, 823, 967
LmnI GCTCC 2 cut(s) 548, 874
LweI GCATC 1 cut(s) 1004
MaeI CTAG 1 cut(s) 711
MaeII ACGT 2 cut(s) 319, 451
MaeIII GTNAC 1 cut(s) 162
MalI GATC 5 cut(s) 27, 31, 783, 825, 969
MboI GATC 5 cut(s) 25, 29, 781, 823, 967
MboII GAAGA 2 cut(s) 25, 94
MflI RGATCY 1 cut(s) 823
MluCI AATT 7 cut(s) 17, 34, 48, 294, 511, 723, 1047
MlyI GAGTC 1 cut(s) 563
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 2 cut(s) 453, 576
MseI TTAA 5 cut(s) 92, 293, 509, 599, 729
MslI CAYNNNNRTG 1 cut(s) 983
MspCI CTTAAG 1 cut(s) 91
MspI CCGG 3 cut(s) 155, 193, 872
MspR9I CCNGG 4 cut(s) 176, 193, 413, 1032
Mva1269I GAATGC 1 cut(s) 990
MvaI CCWGG 3 cut(s) 176, 413, 1032
MwoI GCNNNNNNNGC 4 cut(s) 205, 478, 700, 1019
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 193
NdeII GATC 5 cut(s) 25, 29, 781, 823, 967
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 7 cut(s) 163, 344, 409, 609, 689, 869, 934
NlaIV GGNNCC 5 cut(s) 237, 751, 825, 904, 1057
NspI RCATGY 2 cut(s) 409, 869
PaeI GCATGC 1 cut(s) 869
PaeR7I CTCGAG 1 cut(s) 1007
PagI TCATGA 1 cut(s) 340
PaqCI CACCTGC 1 cut(s) 890
PciI ACATGT 1 cut(s) 405
PctI GAATGC 1 cut(s) 990
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 453, 576
PfeI GAWTC 4 cut(s) 112, 123, 188, 577
PfoI TCCNGGA 2 cut(s) 191, 411
PkrI GCNGC 2 cut(s) 810, 813
Ple19I CGATCG 1 cut(s) 28
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PpuMI RGGWCCY 1 cut(s) 181
PscI ACATGT 1 cut(s) 405
PsiI TTATAA 1 cut(s) 422
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 174, 411, 1030
PspGI CCWGG 3 cut(s) 174, 411, 1030
PspN4I GGNNCC 5 cut(s) 237, 751, 825, 904, 1057
PspPI GGNCC 3 cut(s) 181, 692, 1056
PspPPI RGGWCCY 1 cut(s) 181
PspXI VCTCGAGB 1 cut(s) 1007
PsrI GAACNNNNNNTAC 2 cut(s) 658, 690
PstI CTGCAG 2 cut(s) 463, 945
PsuI RGATCY 1 cut(s) 823
PvuI CGATCG 1 cut(s) 28
RsaI GTAC 4 cut(s) 237, 789, 834, 955
RsaNI GTAC 4 cut(s) 236, 788, 833, 954
RseI CAYNNNNRTG 1 cut(s) 983
SaqAI TTAA 5 cut(s) 92, 293, 509, 599, 729
SatI GCNGC 2 cut(s) 809, 812
Sau3AI GATC 5 cut(s) 25, 29, 781, 823, 967
Sau96I GGNCC 3 cut(s) 181, 692, 1056
ScaI AGTACT 1 cut(s) 834
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 4 cut(s) 176, 193, 413, 1032
SfaNI GCATC 1 cut(s) 1004
SfcI CTRYAG 2 cut(s) 459, 941
Sfr274I CTCGAG 1 cut(s) 1007
SinI GGWCC 1 cut(s) 181
SlaI CTCGAG 1 cut(s) 1007
SmiI ATTTAAAT 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 983
SmlI CTYRAG 5 cut(s) 91, 398, 828, 1007, 1042
SmoI CTYRAG 5 cut(s) 91, 398, 828, 1007, 1042
SphI GCATGC 1 cut(s) 869
Sse9I AATT 7 cut(s) 17, 34, 48, 294, 511, 723, 1047
SsiI CCGC 3 cut(s) 720, 808, 811
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 174, 191, 411, 1030
SwaI ATTTAAAT 1 cut(s) 510
TaaI ACNGT 1 cut(s) 802
TaiI ACGT 2 cut(s) 322, 454
TaqI TCGA 6 cut(s) 28, 80, 334, 468, 998, 1008
TasI AATT 7 cut(s) 17, 34, 48, 294, 511, 723, 1047
TatI WGTACW 1 cut(s) 832
TauI GCSGC 2 cut(s) 811, 814
TfiI GAWTC 4 cut(s) 112, 123, 188, 577
Tru1I TTAA 5 cut(s) 92, 293, 509, 599, 729
Tru9I TTAA 5 cut(s) 92, 293, 509, 599, 729
TscAI CASTG 3 cut(s) 156, 595, 932
TspDTI ATGAA 5 cut(s) 329, 585, 674, 858, 950
TspRI CASTG 3 cut(s) 156, 595, 932
Vha464I CTTAAG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 2 cut(s) 723, 1047
XceI RCATGY 2 cut(s) 409, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 801
XhoI CTCGAG 1 cut(s) 1007
XmnI GAANNNNTTC 2 cut(s) 453, 576
XspI CTAG 1 cut(s) 711
ZrmI AGTACT 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.