Rh2BG559200

APO protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002143
Physical Location & Seq
Forward (+)
76997366 .. 76998770
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG559200.1

Sequence Viewer

Length: 1047 bp
ATGGCGCTGAGGAGAAAATTGGGGGAGAGTTCGAATTTCAGTTGCAAAATGATGTTGAGCTTTTCGAGGTATTACAACTCAGACAAAGTGGACTTAAAGAAGCTGAGGCCTATGATTCTGAAGAGAATCCAGGACCGAGCTAAGGACTATCCGGTGCAGAGCATGATACCAGTGGCCGAGGAGGTCCTTAATTCCCGGAGGCTTCTTATTCAAGGCGTCTCTACCCTTCTCAAAGTGATCCCAGTTGTGGCCTGCAAGTTCTGTCCAGAAGTACACATTAGCGATAAAGGTCACTTAATTCAGACTTGTTGTGGTTTCAAACGTTGTGGCAGAAATCGGGTTCACGAGTGGATAACTGGTAGTTTGAAAGATGTACTTGTTCCAGTAGAGGCATTTCACTTGAAGCACATGTTCCAGGGTGTTATCAAACATAACCAAAGGTTTGATTATGACCGTGTTCCTGCAGTTGTTGAGCTGTGCTGGCAAGCAGGGGCAAATGATGAAAATCTGTATTCCGGTACTTGGAACCCAGAAAGTGATTGTGTTGCTGTTGAAGGAGCTGAGTCATTGTCACCTGCTGAACTCACTCTCGTTGCCAATGGAACTTTGAGGGCCTGGGAAATACTTAGAAATGGCGTGAAGAAATTGTTGATGGTTTATCCAGCGAAAGTGTGTAAAGACTGTTCAGAAGTCCATGTTGGGCCATCTGGGCATAAGGCGAGGCTTTGTGGACTTTTTAAGTATGAGCGTTGGCAAGGAACCCACTTTTGGAGAAAAGCATCTGTAAATGATCTTGTTCCTCCAAACATTGTATGGCGGAGGAGGCGTCAAGATCCTCCTGTGCTTTTGAATGAAGGAAGGGGCTATTATGGGCATGCTCCAGCAGTAGTAGAACTATGCACACAGGCAGGTGCTATTGTGCCTTCAAAGTATCGTTGTATGATGAAAGTAGAGGGTTTAACTTCAGTTGTAAATCAAGGTTTACCCCCTCCGCCATTTAACCTGACAGAACCATACAGGTTACTCCATCCGAAAGATCATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

348

Amino Acids

39.47

Weight (kDa)

9.33

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 29 - 166 2.5e-42 APO RNA-binding
APO_RNA-bind PF05634 194 - 308 5.4e-20 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 583, 899
Acc36I ACCTGC 2 cut(s) 583, 899
AciI CCGC 2 cut(s) 817, 992
AclI AACGTT 1 cut(s) 322
AclWI GGATC 2 cut(s) 232, 827
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 2 cut(s) 140, 948
AcyI GRCGYC 2 cut(s) 216, 826
AfaI GTAC 3 cut(s) 273, 375, 520
AfiI CCNNNNNNNGG 4 cut(s) 142, 247, 522, 768
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 7 cut(s) 212, 319, 367, 403, 554, 850, 927
AjnI CCWGG 3 cut(s) 129, 414, 614
AjuI GAANNNNNNNTTGG 4 cut(s) 681, 713, 751, 783
AluBI AGCT 5 cut(s) 60, 103, 140, 475, 560
AluI AGCT 5 cut(s) 60, 103, 140, 475, 560
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 2 cut(s) 232, 827
AoxI GGCC 5 cut(s) 107, 174, 249, 612, 701
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 4 cut(s) 133, 184, 612, 701
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 1 cut(s) 564
AsuII TTCGAA 1 cut(s) 32
AvaII GGWCC 2 cut(s) 133, 184
BauI CACGAG 1 cut(s) 344
BbvCI CCTCAGC 2 cut(s) 8, 104
BccI CCATC 3 cut(s) 646, 712, 1035
BciT130I CCWGG 3 cut(s) 131, 416, 616
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 223
BfmI CTRYAG 1 cut(s) 462
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 2 cut(s) 583, 899
BglI GCCNNNNNGGC 1 cut(s) 709
Bme1390I CCNGG 4 cut(s) 131, 196, 416, 616
Bme18I GGWCC 2 cut(s) 133, 184
BmgT120I GGNCC 4 cut(s) 133, 184, 612, 701
BmiI GGNNCC 2 cut(s) 527, 760
BmrFI CCNGG 4 cut(s) 131, 196, 416, 616
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 788
BmuI ACTGGG 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 3 cut(s) 8, 104, 141
Bpu14I TTCGAA 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 196
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 2 cut(s) 216, 826
BsaJI CCNNGG 3 cut(s) 177, 415, 615
BsaWI WCCGGW 2 cut(s) 151, 515
BsaXI ACNNNNNCTCC 2 cut(s) 811, 841
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 247, 522, 768
Bse1I ACTGG 4 cut(s) 170, 242, 361, 383
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 3 cut(s) 131, 416, 616
BseDI CCNNGG 3 cut(s) 177, 415, 615
BseGI GGATG 1 cut(s) 1027
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 4 cut(s) 142, 247, 522, 768
BseMII CTCAG 3 cut(s) 93, 95, 552
BseNI ACTGG 4 cut(s) 170, 242, 361, 383
BseRI GAGGAG 3 cut(s) 25, 194, 835
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 5 cut(s) 109, 176, 251, 614, 703
BsiSI CCGG 3 cut(s) 152, 196, 516
BslI CCNNNNNNNGG 4 cut(s) 142, 247, 522, 768
BsmAI GTCTC 1 cut(s) 223
BsmBI CGTCTC 1 cut(s) 223
BsnI GGCC 5 cut(s) 109, 176, 251, 614, 703
Bsp119I TTCGAA 1 cut(s) 32
Bsp143I GATC 4 cut(s) 237, 790, 832, 1036
BspACI CCGC 2 cut(s) 817, 992
BspANI GGCC 5 cut(s) 109, 176, 251, 614, 703
BspCNI CTCAG 3 cut(s) 92, 96, 553
BspLI GGNNCC 2 cut(s) 527, 760
BspMAI CTGCAG 1 cut(s) 466
BspMI ACCTGC 2 cut(s) 583, 899
BspPI GGATC 2 cut(s) 232, 827
BspT104I TTCGAA 1 cut(s) 32
BsrI ACTGG 4 cut(s) 170, 242, 361, 383
BssECI CCNNGG 3 cut(s) 177, 415, 615
BssMI GATC 4 cut(s) 237, 790, 832, 1036
BssNI GRCGYC 2 cut(s) 216, 826
BssSI CACGAG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 3 cut(s) 131, 416, 616
Bst4CI ACNGT 2 cut(s) 455, 683
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 2 cut(s) 216, 826
BstBI TTCGAA 1 cut(s) 32
BstC8I GCNNGC 4 cut(s) 253, 482, 486, 876
BstDEI CTNAG 6 cut(s) 8, 79, 104, 141, 561, 626
BstF5I GGATG 1 cut(s) 1027
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 240, 793, 835, 1039
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 4 cut(s) 237, 790, 832, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 481, 709, 823
BstNI CCWGG 3 cut(s) 131, 416, 616
BstNSI RCATGY 2 cut(s) 412, 878
BstSCI CCNGG 4 cut(s) 129, 194, 414, 614
BstSFI CTRYAG 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
BsuRI GGCC 5 cut(s) 109, 176, 251, 614, 703
BtsCI GGATG 1 cut(s) 1027
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 2 cut(s) 583, 899
Cac8I GCNNGC 4 cut(s) 253, 482, 486, 876
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 133, 184, 612, 701
CseI GACGC 2 cut(s) 205, 815
Csp6I GTAC 3 cut(s) 272, 374, 519
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 5 cut(s) 163, 409, 695, 875, 1040
CviQI GTAC 3 cut(s) 272, 374, 519
DdeI CTNAG 6 cut(s) 8, 79, 104, 141, 561, 626
DpnI GATC 4 cut(s) 239, 792, 834, 1038
DpnII GATC 4 cut(s) 237, 790, 832, 1036
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 2 cut(s) 832, 981
Eco147I AGGCCT 1 cut(s) 109
Eco47I GGWCC 2 cut(s) 133, 184
Eco57I CTGAAG 2 cut(s) 140, 948
EcoO109I RGGNCCY 2 cut(s) 184, 612
EcoRII CCWGG 3 cut(s) 129, 414, 614
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 5 cut(s) 166, 412, 698, 878, 1043
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 5 cut(s) 162, 408, 694, 874, 1039
FokI GGATG 1 cut(s) 1014
GlaI GCGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 864
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 109, 176, 251, 614, 703
HapII CCGG 3 cut(s) 152, 196, 516
HgaI GACGC 2 cut(s) 205, 815
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 216, 826
Hin1II CATG 5 cut(s) 166, 412, 698, 878, 1043
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 3 cut(s) 115, 126, 563
HpaII CCGG 3 cut(s) 152, 196, 516
HphI GGTGA 1 cut(s) 564
Hpy166II GTNNAC 5 cut(s) 91, 274, 343, 731, 983
Hpy188I TCNGA 5 cut(s) 82, 120, 303, 688, 1032
Hpy188III TCNNGA 3 cut(s) 266, 344, 830
Hpy8I GTNNAC 5 cut(s) 91, 274, 343, 731, 983
HpyAV CCTTC 5 cut(s) 236, 548, 848, 852, 933
HpyCH4III ACNGT 2 cut(s) 455, 683
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 5 cut(s) 45, 157, 255, 464, 900
HpyF10VI GCNNNNNNNGC 3 cut(s) 481, 709, 823
HpyF3I CTNAG 6 cut(s) 8, 79, 104, 141, 561, 626
HpySE526I ACGT 1 cut(s) 322
Hsp92I GRCGYC 2 cut(s) 216, 826
Hsp92II CATG 5 cut(s) 166, 412, 698, 878, 1043
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 4 cut(s) 237, 790, 832, 1036
LmnI GCTCC 2 cut(s) 557, 883
LweI GCATC 1 cut(s) 788
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 3 cut(s) 290, 570, 1020
MalI GATC 4 cut(s) 239, 792, 834, 1038
MboI GATC 4 cut(s) 237, 790, 832, 1036
MboII GAAGA 2 cut(s) 133, 652
MflI RGATCY 1 cut(s) 832
MluCI AATT 5 cut(s) 17, 34, 190, 297, 644
MlyI GAGTC 1 cut(s) 572
MseI TTAA 6 cut(s) 95, 189, 296, 738, 959, 999
MspI CCGG 3 cut(s) 152, 196, 516
MspR9I CCNGG 4 cut(s) 131, 196, 416, 616
MvaI CCWGG 3 cut(s) 131, 416, 616
MwoI GCNNNNNNNGC 3 cut(s) 481, 709, 823
NciI CCSGG 1 cut(s) 196
NdeII GATC 4 cut(s) 237, 790, 832, 1036
NlaIII CATG 5 cut(s) 166, 412, 698, 878, 1043
NlaIV GGNNCC 2 cut(s) 527, 760
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 2 cut(s) 290, 570
NspI RCATGY 2 cut(s) 412, 878
NspV TTCGAA 1 cut(s) 32
PaeI GCATGC 1 cut(s) 878
PaqCI CACCTGC 2 cut(s) 583, 899
PceI AGGCCT 1 cut(s) 109
PciI ACATGT 1 cut(s) 408
PfeI GAWTC 2 cut(s) 115, 126
PfoI TCCNGGA 2 cut(s) 129, 194
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 1 cut(s) 408
Psp1406I AACGTT 1 cut(s) 322
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 3 cut(s) 129, 414, 614
PspGI CCWGG 3 cut(s) 129, 414, 614
PspN4I GGNNCC 2 cut(s) 527, 760
PspPI GGNCC 4 cut(s) 133, 184, 612, 701
PspPPI RGGWCCY 1 cut(s) 184
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
PstI CTGCAG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 3 cut(s) 273, 375, 520
RsaNI GTAC 3 cut(s) 272, 374, 519
SaqAI TTAA 6 cut(s) 95, 189, 296, 738, 959, 999
Sau3AI GATC 4 cut(s) 237, 790, 832, 1036
Sau96I GGNCC 4 cut(s) 133, 184, 612, 701
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 4 cut(s) 131, 196, 416, 616
SfaNI GCATC 1 cut(s) 788
SfcI CTRYAG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 32
SinI GGWCC 2 cut(s) 133, 184
SphI GCATGC 1 cut(s) 878
Sse9I AATT 5 cut(s) 17, 34, 190, 297, 644
SseBI AGGCCT 1 cut(s) 109
SsiI CCGC 2 cut(s) 817, 992
StuI AGGCCT 1 cut(s) 109
StyD4I CCNGG 4 cut(s) 129, 194, 414, 614
TaaI ACNGT 2 cut(s) 455, 683
TaiI ACGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 32, 65
TaqII GACCGA 1 cut(s) 150
TasI AATT 5 cut(s) 17, 34, 190, 297, 644
TatI WGTACW 2 cut(s) 271, 373
TfiI GAWTC 2 cut(s) 115, 126
Tru1I TTAA 6 cut(s) 95, 189, 296, 738, 959, 999
Tru9I TTAA 6 cut(s) 95, 189, 296, 738, 959, 999
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 2 cut(s) 290, 570
Tsp45I GTSAC 2 cut(s) 290, 570
TspDTI ATGAA 3 cut(s) 516, 867, 959
TspRI CASTG 1 cut(s) 177
VpaK11BI GGWCC 2 cut(s) 133, 184
XapI RAATTY 1 cut(s) 34
XceI RCATGY 2 cut(s) 412, 878
XcmI CCANNNNNNNNNTGG 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.