Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001659

Physical Location & Seq

Genomic Coordinates Forward (+)

27853934 .. 27857826

Gene Structure 3893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1197300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGATGGACATTAAGGCTGTTTCGTTCGCCGCCGAGAACCCTCCGCTCGCGGAGCTCCTCGCCGCCAAGCTCGCTGGAATCTCTCTCCCCACCGACGCCTCTCTCCCTCCCGATTCCCTTTATTCTTTGCAATTCTCCAATGGGTTGAAATTGCATGGAAAAAATGTACTTCTCCGATACATCGGCCGAATTGCGAAATTGTATGGTGACAATCCATTCGAAGCCGGGAAGATTGATGAGTGGCTTGAGTACGCGCCTGTGCTTTCACAGGGTTCTGCGTTTGAGAGTGCTTGTAAACATTTGGATGACTATCTGGGAAGCAGTACCTTTTTTGCTAATCAATCTTTGTCCATCGCCGATATCGCAATCTGGTCAGGTCTTGCAGGAACTGGGAAAAGATGGGAGGGTTTGAGGAATTCAAAGAAGTATCCAAACCTTTCTCGGTGGTTCAATTCACTATTAGCCGAATATGGTGATGCCCTGAATGAAGTCACAGCTACCTTCATTGGCAAGAAGGGCTCAGGAAAGCCAACCGCAACTAAAACAAAAGGCACTCAGGCCGATCAGCAGTTGAAGAGTGTAAATGGAGATGTATCTGAGAAAGGTAAAGCAGGAAGCAAACCAACATCTGAAGTAGATCTTCCAGAAGCGGAGGTTGGAAAGGTTCGACTGAGATTTGCACCAGAGCCCAGTGGTTATCTTCACATTGGGCACTCAAAAGCAGCATTATTGAATCAGTATTTTGCTCAGCGGTATAAGGGTCAGCTTATTGTGCGGTTTGACGATACAAATCCTGATAAAGAAAGCAATGAGTTTGTGGACAATCTTCTCAAGGATATTGAGACTTTGGGTATCAAGTATGAAACGGTTACCTATACTTCAGATTACTTCCCACAGTTGATGGAAATGGCTGAGAATCTGATTCGCCAGGGTAAAGCATATGTTGATGATACGCCACGTGAGGAGATGAAGAAAGAAAGGATGGATGGCATTGAATCAAAATGTAGAAACAACAGTGTAGAGGAGAATCTAAAATTATGGAATGAAATGATTGCTGGGTCTAAGAGGGGTAAGGAGTGCTGTGTCCGTGGGAAGCTGGATATGCAGGATCCAAACAAATCTCTTCGAGATCCTGTTTATTATCGCTGCAATCCAACACCACATCATCGGATTGGGTCCAAGTACAACTTATACCCAACTTATGATTTTGCTTGTCCATTTGTTGATTCTATAGAAGGTATTACACATGCACTTCGATCTAGTGAGTATCACGACCGCAATGCTCAGTACTATCGAGTTCAAGAGGATATGGGACTGCGAAAAGTGCATATCTATGAATTCAGTAGGTTGAACATGGTTTATACACTTCTCAGTAAGCGCAAGCTTTTGTGGTTCGTTCAGAATGAGAAGGTTAGTGGATGGGACGATCCTCGTTTTCCAACTGTCCAAGGAATTGTGCGCAGAGGTCTCAAAGTTGAAGCATTAATACAATTCATTCTTGAGCAGGGGGCTTCAAAAAATCTGAATCTCATGGAATGGGACAAACTCTGGACTATTAATAAGAAGATAATTGATCCTGTATGCCCAAGACATACTGCCGTCATCGAAGAGCGGCGCGTGTTGTTGACCCTGACTAATGGTCCTGAGAAACCATTTGTCCGCATCATTCCTAGGCATAAGAAGTACGAAGGTGCTGGGGAGAAGGCAACAACATACACAAGGAGGATATGGCTAGATTATGCTGATGCGGAGTCCATCAAAGTAGACGAGGAAGTAACCCTAATGGATTGGGGGAACGCCATTGTTAAGGGAATTGAGAAAGACACTGACGGAAATCTCAAACTCACAGGGGTCTTGCATCCTGAGGGTTCTGTCAAGACCACAAAATTGAAGCTCACCTGGCTCCCTGAATCAGATGAGCTAGTTAAGCTCTCATTGATGGAGTTTGATCATCTGATAACAAAGAAGAAGCTCGAGGAAGATGAGGATTTCGTCGATGTTGTTAACCCATGTACCGAAAAGGAGACCGCAGCGCTTGGGGATTCCAACATGCGAAACCTTAAGTGTGGGGATATATTGCAGCTGGAGAGGAAAGGATATTTCCGATGCGATGTTCCCTACCTCAGGCCTTCAAAACCCGTTGTTCTATTTGCTATCCCCGACGGCAGGCAGCAGACTGGATCGAAGGACCTGATTTCTGCACTTCACGTGTGCCTGCATGCTCCTTTCTGGCTTTTGTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002181 GO:0003674 GO:0003824 GO:0004812 GO:0004818 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006424 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00860 ko00970 ko01100 ko01110 ko01120 map00860 map00970 map01100 map01110 map01120

Pfam Domains

Protein Families

GST_C GST_C_3 tRNA-synt_1c tRNA-synt_1c_C

Protein Analysis

748

Amino Acids

84.62

Weight (kDa)

7.21

Isoelectric Point (pI)

32.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1c	PF00749	221 - 525	1.4e-107	tRNA synthetases class I (E and Q), catalytic domain
tRNA-synt_1c_C	PF03950	528 - 613	2e-14	tRNA synthetases class I (E and Q), anti-codon binding domain
tRNA-synt_1c_C2	PF20974	631 - 704	1.3e-14	tRNA synthetases class I (E and Q), anti-codon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001659)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26710
fragaria_vesca	FvH4_1g07040 FvH4_3g18420 FvH4_5g29940 FvH4_5g29940 FvH4_6g08750
malus_domestica	MD04G1184100.v1.1 MD12G1197300.v1.1
prunus_persica	Prupe.6G302600_v2.0.a1 Prupe.6G302600_v2.0.a1
pyrus_communis	pycom04g16310
rosa_chinensis	RchiOBHm_Chr1g0323481 RchiOBHm_Chr1g0323531 RchiOBHm_Chr1g0330311 RchiOBHm_Chr1g0330321 RchiOBHm_Chr1g0330421 RchiOBHm_Chr1g0330431 RchiOBHm_Chr3g0459011
rosa_laevigata	RLG00000025126 RLG00000029811 RLG00000030323 RLG00000030332
rosa_multiflora	Rmu_co8493977.1_g000001 Rmu_sc0002634.1_g000044 Rmu_sc0004343.1_g000022 Rmu_sc0007290.1_g000009 Rmu_sc0007290.1_g000010
rosa_roxburghii	Rroxscaffold_4G00319950 Rroxscaffold_4G00319960 Rroxscaffold_6G00420600
rosa_rugosa	Rorug01G0088900 Rorug03G0032500 Rorug05G0414800
rosa_samantha	Rh1AG106700 Rh1AG106800 Rh1CG056600 Rh1CG057900 Rh1CG102700 Rh1CG102800 Rh1DG060600 Rh1DG061700 Rh1DG110600 Rh3BG093600 Rh5DG502200 Rh6BG457000 Rh6BG457100
rosa_wichuraiana	Rw1G008430 Rw1G008550 Rw3G007770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1460
AccBSI	CCGCTC	2 cut(s)	46, 1612
AccI	GTMKAC	1 cut(s)	1764
AccII	CGCG	3 cut(s)	50, 254, 1617
AclWI	GGATC	6 cut(s)	1103, 1116, 1124, 1421, 1568, 2188
AcoI	YGGCCR	1 cut(s)	184
AcsI	RAATTY	2 cut(s)	415, 1337
AcuI	CTGAAG	2 cut(s)	651, 864
AcvI	CACGTG	2 cut(s)	959, 2209
AcyI	GRCGYC	1 cut(s)	96
AfaI	GTAC	7 cut(s)	168, 251, 325, 1184, 1289, 1685, 2014
AfeI	AGCGCT	1 cut(s)	2034
AfiI	CCNNNNNNNGG	2 cut(s)	2124, 2166
AflII	CTTAAG	1 cut(s)	2060
AflIII	ACRYGT	1 cut(s)	2208
AhdI	GACNNNNNGTC	1 cut(s)	1638
AjnI	CCWGG	2 cut(s)	927, 1898
AjuI	GAANNNNNNNTTGG	2 cut(s)	639, 671
Alw21I	GWGCWC	1 cut(s)	57
Alw26I	GTCTC	3 cut(s)	836, 1472, 2018
AlwI	GGATC	6 cut(s)	1103, 1116, 1124, 1421, 1568, 2188
AlwNI	CAGNNNCTG	1 cut(s)	389
Ama87I	CYCGRG	1 cut(s)	1973
Aor51HI	AGCGCT	1 cut(s)	2034
AoxI	GGCC	3 cut(s)	184, 558, 2126
ApeKI	GCWGC	5 cut(s)	722, 1146, 2030, 2080, 2170
ApoI	RAATTY	2 cut(s)	415, 1337
ArsI	GACNNNNNNTTYG	2 cut(s)	200, 232
AseI	ATTAAT	2 cut(s)	1484, 1557
AspA2I	CCTAGG	1 cut(s)	1670
AspLEI	GCGC	5 cut(s)	256, 1380, 1461, 1617, 2035
AspS9I	GGNCC	3 cut(s)	1176, 1640, 2188
AsuC2I	CCSGG	1 cut(s)	226
AsuHPI	GGTGA	3 cut(s)	218, 485, 1888
AsuII	TTCGAA	1 cut(s)	219
AvaI	CYCGRG	1 cut(s)	1973
AvaII	GGWCC	3 cut(s)	1176, 1640, 2188
AvrII	CCTAGG	1 cut(s)	1670
AxyI	CCTNAGG	2 cut(s)	1863, 2123
BaeGI	GKGCMC	1 cut(s)	714
BaeI	ACNNNNGTAYC	2 cut(s)	1996, 2029
BamHI	GGATCC	1 cut(s)	1108
BanII	GRGCYC	3 cut(s)	57, 521, 690
BarI	GAAGNNNNNNTAC	2 cut(s)	1470, 1502
BbrPI	CACGTG	2 cut(s)	959, 2209
Bbv12I	GWGCWC	1 cut(s)	57
BbvI	GCAGC	5 cut(s)	734, 1133, 2042, 2092, 2182
BccI	CCATC	8 cut(s)	359, 393, 895, 976, 980, 1413, 1763, 1933
BceAI	ACGGC	2 cut(s)	1583, 2179
BcgI	CGANNNNNNTGC	2 cut(s)	1262, 1296
BciT130I	CCWGG	2 cut(s)	929, 1900
BciVI	GTATCC	1 cut(s)	438
BclI	TGATCA	1 cut(s)	1948
BcnI	CCSGG	1 cut(s)	226
BcoDI	GTCTC	3 cut(s)	836, 1472, 2018
BfaI	CTAG	4 cut(s)	1260, 1671, 1733, 1922
BfmI	CTRYAG	1 cut(s)	1230
BfoI	RGCGCY	1 cut(s)	2036
BfrI	CTTAAG	1 cut(s)	2060
BfuI	GTATCC	1 cut(s)	438
BglII	AGATCT	1 cut(s)	637
BisI	GCNGC	8 cut(s)	30, 63, 723, 1147, 1613, 2031, 2081, 2171
BlnI	CCTAGG	1 cut(s)	1670
BlpI	GCTNAGC	1 cut(s)	747
BlsI	GCNGC	8 cut(s)	31, 64, 724, 1148, 1614, 2032, 2082, 2172
BmcAI	AGTACT	1 cut(s)	1289
Bme1390I	CCNGG	3 cut(s)	226, 929, 1900
Bme18I	GGWCC	3 cut(s)	1176, 1640, 2188
BmeRI	GACNNNNNGTC	1 cut(s)	1638
BmeT110I	CYCGRG	1 cut(s)	1973
BmgT120I	GGNCC	3 cut(s)	1176, 1640, 2188
BmiI	GGNNCC	3 cut(s)	1110, 1177, 1904
BmrFI	CCNGG	3 cut(s)	226, 929, 1900
BmrI	ACTGGG	2 cut(s)	399, 684
BmsI	GCATC	5 cut(s)	466, 1671, 1735, 1867, 2096
BmuI	ACTGGG	2 cut(s)	399, 684
BpmI	CTGGAG	1 cut(s)	2105
Bpu10I	CCTNAGC	1 cut(s)	520
Bpu1102I	GCTNAGC	1 cut(s)	747
Bpu14I	TTCGAA	1 cut(s)	219
BpuEI	CTTGAG	3 cut(s)	266, 815, 1520
BpuMI	CCSGG	1 cut(s)	226
BsaAI	YACGTR	2 cut(s)	959, 2209
BsaBI	GATNNNNATC	2 cut(s)	309, 789
BsaHI	GRCGYC	1 cut(s)	96
BsaI	GGTCTC	2 cut(s)	1472, 2018
BsaJI	CCNNGG	4 cut(s)	928, 1087, 1447, 1670
Bsc4I	CCNNNNNNNGG	2 cut(s)	2124, 2166
Bse1I	ACTGG	3 cut(s)	394, 690, 2182
Bse21I	CCTNAGG	2 cut(s)	1863, 2123
Bse3DI	GCAATG	2 cut(s)	814, 1285
Bse8I	GATNNNNATC	2 cut(s)	309, 789
BseBI	CCWGG	2 cut(s)	929, 1900
BseDI	CCNNGG	4 cut(s)	928, 1087, 1447, 1670
BseGI	GGATG	5 cut(s)	310, 987, 991, 1424, 1858
BseJI	GATNNNNATC	2 cut(s)	309, 789
BseLI	CCNNNNNNNGG	2 cut(s)	2124, 2166
BseMI	GCAATG	2 cut(s)	814, 1285
BseNI	ACTGG	3 cut(s)	394, 690, 2182
BseRI	GAGGAG	3 cut(s)	47, 977, 1037
BseSI	GKGCMC	1 cut(s)	714
BseX3I	CGGCCG	1 cut(s)	184
BseXI	GCAGC	5 cut(s)	734, 1133, 2042, 2092, 2182
BseYI	CCCAGC	2 cut(s)	1055, 1694
BsgI	GTGCAG	1 cut(s)	2184
Bsh1236I	CGCG	3 cut(s)	50, 254, 1617
Bsh1285I	CGRYCG	2 cut(s)	187, 1276
BshFI	GGCC	3 cut(s)	186, 560, 2128
BsiEI	CGRYCG	2 cut(s)	187, 1276
BsiHKAI	GWGCWC	1 cut(s)	57
BsiHKCI	CYCGRG	1 cut(s)	1973
BsiSI	CCGG	1 cut(s)	225
BslFI	GGGAC	3 cut(s)	1326, 1436, 1553
BslI	CCNNNNNNNGG	2 cut(s)	2124, 2166
BsmAI	GTCTC	3 cut(s)	836, 1472, 2018
BsmFI	GGGAC	3 cut(s)	1326, 1436, 1553
BsnI	GGCC	3 cut(s)	186, 560, 2128
Bso31I	GGTCTC	2 cut(s)	1472, 2018
BsoBI	CYCGRG	1 cut(s)	1973
Bsp119I	TTCGAA	1 cut(s)	219
Bsp1286I	GDGCHC	4 cut(s)	57, 521, 690, 714
Bsp143I	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
Bsp1720I	GCTNAGC	1 cut(s)	747
BspANI	GGCC	3 cut(s)	186, 560, 2128
BspFNI	CGCG	3 cut(s)	50, 254, 1617
BspLI	GGNNCC	3 cut(s)	1110, 1177, 1904
BspPI	GGATC	6 cut(s)	1103, 1116, 1124, 1421, 1568, 2188
BspQI	GCTCTTC	1 cut(s)	1602
BspT104I	TTCGAA	1 cut(s)	219
BspTI	CTTAAG	1 cut(s)	2060
BspTNI	GGTCTC	2 cut(s)	1472, 2018
BsrBI	CCGCTC	2 cut(s)	46, 1612
BsrDI	GCAATG	2 cut(s)	814, 1285
BsrI	ACTGG	3 cut(s)	394, 690, 2182
BssECI	CCNNGG	4 cut(s)	928, 1087, 1447, 1670
BssMI	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
BssNI	GRCGYC	1 cut(s)	96
BssT1I	CCWWGG	2 cut(s)	1447, 1670
Bst2UI	CCWGG	2 cut(s)	929, 1900
Bst4CI	ACNGT	4 cut(s)	868, 897, 1016, 1444
Bst6I	CTCTTC	3 cut(s)	569, 1128, 1602
BstACI	GRCGYC	1 cut(s)	96
BstAFI	CTTAAG	1 cut(s)	2060
BstBAI	YACGTR	2 cut(s)	959, 2209
BstBI	TTCGAA	1 cut(s)	219
BstC8I	GCNNGC	6 cut(s)	48, 72, 1382, 2168, 2216, 2220
BstDSI	CCRYGG	1 cut(s)	1087
BstEII	GGTNACC	1 cut(s)	868
BstENI	CCTNNNNNAGG	1 cut(s)	2122
BstF5I	GGATG	5 cut(s)	310, 987, 991, 1424, 1858
BstFNI	CGCG	3 cut(s)	50, 254, 1617
BstH2I	RGCGCY	1 cut(s)	2036
BstHHI	GCGC	5 cut(s)	256, 1380, 1461, 1617, 2035
BstKTI	GATC	9 cut(s)	565, 640, 1111, 1132, 1259, 1429, 1576, 1951, 2183
BstMAI	GTCTC	3 cut(s)	836, 1472, 2018
BstMBI	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
BstMCI	CGRYCG	2 cut(s)	187, 1276
BstMWI	GCNNNNNNNGC	9 cut(s)	52, 71, 362, 516, 772, 1102, 1324, 1900, 1927
BstNI	CCWGG	2 cut(s)	929, 1900
BstNSI	RCATGY	3 cut(s)	1250, 2053, 2222
BstPI	GGTNACC	1 cut(s)	868
BstSCI	CCNGG	3 cut(s)	224, 927, 1898
BstSFI	CTRYAG	1 cut(s)	1230
BstSLI	GKGCMC	1 cut(s)	714
BstUI	CGCG	3 cut(s)	50, 254, 1617
BstV1I	GCAGC	5 cut(s)	734, 1133, 2042, 2092, 2182
BstX2I	RGATCY	3 cut(s)	637, 1108, 1129
BstYI	RGATCY	3 cut(s)	637, 1108, 1129
BstZI	CGGCCG	1 cut(s)	184
Bsu36I	CCTNAGG	2 cut(s)	1863, 2123
BsuI	GTATCC	1 cut(s)	438
BsuRI	GGCC	3 cut(s)	186, 560, 2128
BtgI	CCRYGG	1 cut(s)	1087
BtgZI	GCGATG	2 cut(s)	337, 2124
BtsCI	GGATG	5 cut(s)	310, 987, 991, 1424, 1858
BtsIMutI	CAGTG	3 cut(s)	697, 1021, 1824
Cac8I	GCNNGC	6 cut(s)	48, 72, 1382, 2168, 2216, 2220
CaiI	CAGNNNCTG	1 cut(s)	389
CfoI	GCGC	5 cut(s)	256, 1380, 1461, 1617, 2035
Cfr13I	GGNCC	3 cut(s)	1176, 1640, 2188
CseI	GACGC	1 cut(s)	104
Csp6I	GTAC	7 cut(s)	167, 250, 324, 1183, 1288, 1684, 2013
CviAII	CATG	7 cut(s)	155, 1247, 1354, 1531, 2010, 2050, 2219
CviQI	GTAC	7 cut(s)	167, 250, 324, 1183, 1288, 1684, 2013
DpnI	GATC	9 cut(s)	564, 639, 1110, 1131, 1258, 1428, 1575, 1950, 2182
DpnII	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
DriI	GACNNNNNGTC	1 cut(s)	1638
EaeI	YGGCCR	1 cut(s)	184
EagI	CGGCCG	1 cut(s)	184
Eam1104I	CTCTTC	3 cut(s)	569, 1128, 1602
Eam1105I	GACNNNNNGTC	1 cut(s)	1638
EarI	CTCTTC	3 cut(s)	569, 1128, 1602
Ecl136II	GAGCTC	1 cut(s)	55
EclXI	CGGCCG	1 cut(s)	184
Eco130I	CCWWGG	2 cut(s)	1447, 1670
Eco147I	AGGCCT	1 cut(s)	2128
Eco24I	GRGCYC	3 cut(s)	57, 521, 690
Eco31I	GGTCTC	2 cut(s)	1472, 2018
Eco32I	GATATC	1 cut(s)	361
Eco47I	GGWCC	3 cut(s)	1176, 1640, 2188
Eco47III	AGCGCT	1 cut(s)	2034
Eco52I	CGGCCG	1 cut(s)	184
Eco53kI	GAGCTC	1 cut(s)	55
Eco57I	CTGAAG	2 cut(s)	651, 864
Eco72I	CACGTG	2 cut(s)	959, 2209
Eco81I	CCTNAGG	2 cut(s)	1863, 2123
Eco88I	CYCGRG	1 cut(s)	1973
Eco91I	GGTNACC	1 cut(s)	868
EcoICRI	GAGCTC	1 cut(s)	55
EcoNI	CCTNNNNNAGG	1 cut(s)	2122
EcoO109I	RGGNCCY	1 cut(s)	2188
EcoO65I	GGTNACC	1 cut(s)	868
EcoRI	GAATTC	2 cut(s)	415, 1337
EcoRII	CCWGG	2 cut(s)	927, 1898
EcoRV	GATATC	1 cut(s)	361
EcoT14I	CCWWGG	2 cut(s)	1447, 1670
EcoT38I	GRGCYC	3 cut(s)	57, 521, 690
ErhI	CCWWGG	2 cut(s)	1447, 1670
FaeI	CATG	7 cut(s)	158, 1250, 1357, 1534, 2013, 2053, 2222
FalI	AAGNNNNNCTT	4 cut(s)	624, 656, 1172, 1204
FaqI	GGGAC	3 cut(s)	1326, 1436, 1553
FatI	CATG	7 cut(s)	154, 1246, 1353, 1530, 2009, 2049, 2218
FauNDI	CATATG	1 cut(s)	940
FbaI	TGATCA	1 cut(s)	1948
FblI	GTMKAC	1 cut(s)	1764
Fnu4HI	GCNGC	8 cut(s)	30, 63, 723, 1147, 1613, 2031, 2081, 2171
FokI	GGATG	5 cut(s)	317, 994, 998, 1431, 1845
FriOI	GRGCYC	3 cut(s)	57, 521, 690
Fsp4HI	GCNGC	8 cut(s)	30, 63, 723, 1147, 1613, 2031, 2081, 2171
FspBI	CTAG	4 cut(s)	1260, 1671, 1733, 1922
FspI	TGCGCA	1 cut(s)	1460
GlaI	GCGC	5 cut(s)	255, 1379, 1460, 1616, 2034
GluI	GCNGC	8 cut(s)	30, 63, 723, 1147, 1613, 2031, 2081, 2171
GsaI	CCCAGC	2 cut(s)	1059, 1698
GsuI	CTGGAG	1 cut(s)	2105
HaeII	RGCGCY	1 cut(s)	2036
HaeIII	GGCC	3 cut(s)	186, 560, 2128
HapII	CCGG	1 cut(s)	225
HgaI	GACGC	1 cut(s)	104
HhaI	GCGC	5 cut(s)	256, 1380, 1461, 1617, 2035
Hin1I	GRCGYC	1 cut(s)	96
Hin1II	CATG	7 cut(s)	158, 1250, 1357, 1534, 2013, 2053, 2222
Hin6I	GCGC	5 cut(s)	254, 1378, 1459, 1615, 2033
HinP1I	GCGC	5 cut(s)	254, 1378, 1459, 1615, 2033
HincII	GTYRAC	2 cut(s)	1626, 2005
HindII	GTYRAC	2 cut(s)	1626, 2005
HindIII	AAGCTT	1 cut(s)	1382
HpaI	GTTAAC	1 cut(s)	2005
HpaII	CCGG	1 cut(s)	225
HphI	GGTGA	3 cut(s)	218, 485, 1888
Hpy166II	GTNNAC	5 cut(s)	296, 820, 1626, 1765, 2005
Hpy8I	GTNNAC	5 cut(s)	296, 820, 1626, 1765, 2005
Hpy99I	CGWCG	3 cut(s)	98, 1997, 2165
HpyAV	CCTTC	8 cut(s)	508, 511, 1229, 1402, 1682, 1696, 2139, 2179
HpyCH4III	ACNGT	4 cut(s)	868, 897, 1016, 1444
HpyCH4IV	ACGT	2 cut(s)	958, 2208
HpyF10VI	GCNNNNNNNGC	9 cut(s)	52, 71, 362, 516, 772, 1102, 1324, 1900, 1927
HpySE526I	ACGT	2 cut(s)	958, 2208
Hsp92I	GRCGYC	1 cut(s)	96
Hsp92II	CATG	7 cut(s)	158, 1250, 1357, 1534, 2013, 2053, 2222
HspAI	GCGC	5 cut(s)	254, 1378, 1459, 1615, 2033
Ksp22I	TGATCA	1 cut(s)	1948
KspAI	GTTAAC	1 cut(s)	2005
Kzo9I	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
LguI	GCTCTTC	1 cut(s)	1602
LmnI	GCTCC	4 cut(s)	52, 60, 1908, 2227
Lsp1109I	GCAGC	5 cut(s)	734, 1133, 2042, 2092, 2182
LweI	GCATC	5 cut(s)	466, 1671, 1735, 1867, 2096
MaeI	CTAG	4 cut(s)	1260, 1671, 1733, 1922
MaeII	ACGT	2 cut(s)	958, 2208
MaeIII	GTNAC	4 cut(s)	206, 490, 868, 1774
MalI	GATC	9 cut(s)	564, 639, 1110, 1131, 1258, 1428, 1575, 1950, 2182
MbiI	CCGCTC	2 cut(s)	46, 1612
MboI	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
MflI	RGATCY	3 cut(s)	637, 1108, 1129
MhlI	GDGCHC	4 cut(s)	57, 521, 690, 714
MlyI	GAGTC	1 cut(s)	1760
MmeI	TCCRAC	4 cut(s)	637, 1178, 1463, 2070
MseI	TTAA	7 cut(s)	12, 1484, 1557, 1806, 1926, 2004, 2061
MslI	CAYNNNNRTG	2 cut(s)	303, 1332
MspA1I	CMGCKG	2 cut(s)	751, 2083
MspCI	CTTAAG	1 cut(s)	2060
MspI	CCGG	1 cut(s)	225
MspR9I	CCNGG	3 cut(s)	226, 929, 1900
MvaI	CCWGG	2 cut(s)	929, 1900
MvnI	CGCG	3 cut(s)	50, 254, 1617
MwoI	GCNNNNNNNGC	9 cut(s)	52, 71, 362, 516, 772, 1102, 1324, 1900, 1927
NciI	CCSGG	1 cut(s)	226
NdeI	CATATG	1 cut(s)	940
NdeII	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
NlaIII	CATG	7 cut(s)	158, 1250, 1357, 1534, 2013, 2053, 2222
NlaIV	GGNNCC	3 cut(s)	1110, 1177, 1904
NmeAIII	GCCGAG	1 cut(s)	58
NmuCI	GTSAC	2 cut(s)	206, 490
NsbI	TGCGCA	1 cut(s)	1460
NspI	RCATGY	3 cut(s)	1250, 2053, 2222
NspV	TTCGAA	1 cut(s)	219
PaeI	GCATGC	1 cut(s)	2222
PaeR7I	CTCGAG	1 cut(s)	1973
PceI	AGGCCT	1 cut(s)	2128
PciSI	GCTCTTC	1 cut(s)	1602
PkrI	GCNGC	8 cut(s)	31, 64, 724, 1148, 1614, 2032, 2082, 2172
PleI	GAGTC	1 cut(s)	1759
PmaCI	CACGTG	2 cut(s)	959, 2209
PmlI	CACGTG	2 cut(s)	959, 2209
PpsI	GAGTC	1 cut(s)	1759
Ppu21I	YACGTR	2 cut(s)	959, 2209
PpuMI	RGGWCCY	1 cut(s)	2188
PshBI	ATTAAT	2 cut(s)	1484, 1557
Psp124BI	GAGCTC	1 cut(s)	57
Psp5II	RGGWCCY	1 cut(s)	2188
Psp6I	CCWGG	2 cut(s)	927, 1898
PspCI	CACGTG	2 cut(s)	959, 2209
PspEI	GGTNACC	1 cut(s)	868
PspFI	CCCAGC	2 cut(s)	1055, 1694
PspGI	CCWGG	2 cut(s)	927, 1898
PspN4I	GGNNCC	3 cut(s)	1110, 1177, 1904
PspPI	GGNCC	3 cut(s)	1176, 1640, 2188
PspPPI	RGGWCCY	1 cut(s)	2188
PspXI	VCTCGAGB	1 cut(s)	1973
PstNI	CAGNNNCTG	1 cut(s)	389
PsuI	RGATCY	3 cut(s)	637, 1108, 1129
PvuII	CAGCTG	1 cut(s)	2083
RsaI	GTAC	7 cut(s)	168, 251, 325, 1184, 1289, 1685, 2014
RsaNI	GTAC	7 cut(s)	167, 250, 324, 1183, 1288, 1684, 2013
RseI	CAYNNNNRTG	2 cut(s)	303, 1332
SacI	GAGCTC	1 cut(s)	57
SapI	GCTCTTC	1 cut(s)	1602
SaqAI	TTAA	7 cut(s)	12, 1484, 1557, 1806, 1926, 2004, 2061
SatI	GCNGC	8 cut(s)	30, 63, 723, 1147, 1613, 2031, 2081, 2171
Sau3AI	GATC	9 cut(s)	562, 637, 1108, 1129, 1256, 1426, 1573, 1948, 2180
Sau96I	GGNCC	3 cut(s)	1176, 1640, 2188
ScaI	AGTACT	1 cut(s)	1289
SchI	GAGTC	1 cut(s)	1760
ScrFI	CCNGG	3 cut(s)	226, 929, 1900
SduI	GDGCHC	4 cut(s)	57, 521, 690, 714
SfaNI	GCATC	5 cut(s)	466, 1671, 1735, 1867, 2096
SfcI	CTRYAG	1 cut(s)	1230
Sfr274I	CTCGAG	1 cut(s)	1973
SfuI	TTCGAA	1 cut(s)	219
SinI	GGWCC	3 cut(s)	1176, 1640, 2188
SlaI	CTCGAG	1 cut(s)	1973
SmiMI	CAYNNNNRTG	2 cut(s)	303, 1332
SmlI	CTYRAG	5 cut(s)	245, 830, 1499, 1973, 2060
SmoI	CTYRAG	5 cut(s)	245, 830, 1499, 1973, 2060
SphI	GCATGC	1 cut(s)	2222
SseBI	AGGCCT	1 cut(s)	2128
SspMI	CTAG	4 cut(s)	1260, 1671, 1733, 1922
SstI	GAGCTC	1 cut(s)	57
StuI	AGGCCT	1 cut(s)	2128
StyD4I	CCNGG	3 cut(s)	224, 927, 1898
StyI	CCWWGG	2 cut(s)	1447, 1670
TaaI	ACNGT	4 cut(s)	868, 897, 1016, 1444
TaiI	ACGT	2 cut(s)	961, 2211
TaqI	TCGA	9 cut(s)	219, 667, 1126, 1255, 1294, 1605, 1974, 1995, 2183
TatI	WGTACW	3 cut(s)	166, 1182, 1287
TauI	GCSGC	3 cut(s)	32, 65, 1615
Tru1I	TTAA	7 cut(s)	12, 1484, 1557, 1806, 1926, 2004, 2061
Tru9I	TTAA	7 cut(s)	12, 1484, 1557, 1806, 1926, 2004, 2061
TscAI	CASTG	3 cut(s)	697, 1021, 1831
TseFI	GTSAC	2 cut(s)	206, 490
TseI	GCWGC	5 cut(s)	722, 1146, 2030, 2080, 2170
Tsp45I	GTSAC	2 cut(s)	206, 490
TspDTI	ATGAA	7 cut(s)	493, 501, 876, 983, 1059, 1350, 1483
TspGWI	ACGGA	2 cut(s)	1076, 1845
TspRI	CASTG	3 cut(s)	697, 1021, 1831
Vha464I	CTTAAG	1 cut(s)	2060
VpaK11BI	GGWCC	3 cut(s)	1176, 1640, 2188
VspI	ATTAAT	2 cut(s)	1484, 1557
XagI	CCTNNNNNAGG	1 cut(s)	2122
XapI	RAATTY	2 cut(s)	415, 1337
XceI	RCATGY	3 cut(s)	1250, 2053, 2222
XhoI	CTCGAG	1 cut(s)	1973
XmaJI	CCTAGG	1 cut(s)	1670
XmiI	GTMKAC	1 cut(s)	1764
XspI	CTAG	4 cut(s)	1260, 1671, 1733, 1922
ZrmI	AGTACT	1 cut(s)	1289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.