Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006138

Physical Location & Seq

Genomic Coordinates Reverse (-)

5584048 .. 5585895

Gene Structure 1848 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1079400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1848 bp

ATGAGCTCCCTAAGATGCATTGCAAAACACCCAACAATTGCCCTAGTAGACGCATACAGCACCCTCAGAGAGCTCAAGCAAATCCACTCCCAACTGCTCGTTAATGGCCTTCTCAACGACCGTCACCTTTCCGGCCACTTTGTCGCCGCTATTGCCCTCAGAAACCCCATCAATTTGGATTATTCCAATCAAGTCCTCCACCAATGCCAGAACCCAACACTCTTCGCCTTCAACTCCATGATCAGAGCCTACTCCAAAAGCTCCACGCCATCCAAAAGTTTTCACTTTTACAGCAGAATTCTCCGATCAAGTGACAATCTTTCACCCGATAATTACACCTTCAACTTCTTGGTTCGCACTTGCGCGCAGCTTTTGGCACGTGAGACCGGTCCTACCGTCCATGGCGGATTGATAAAGCACGGGTTTGAGAACGACCCACATGTCCAAAGCGGGTTAATTTTCATGTATGCTGAATTGGGTTCTTTAGATCCGTGTCACAGAGTGTTTAGAGAAATTGTTGAGCCGGATTTGGTTTGCCAGACGGCTATGGTGAGTGCTTGTGCGAGATGCGGTGACGTTGGTTTTGCACGGGACCTGTTTGATGAAATGCCTCAGAGGGATCCCGTTGCTTGGAATGCGATGATTGCAGGGTATGCGCAATGTGGGAAGTCCAGGGAAGCCTTGAATCTGTTTCATTTGATGCAAATGGAGGGTGTGAGGGTCAATGAGGTGTCTATGGTTTCGGTTTTATCGGCTTGCTCCCACTTGGGGGCATTGGATCAAGGGAGATGGGCTCATGCCTATATAGAGAAGAACAAGGTGCGGATGACGGTGACGCTGGGGACTGCACTCATCGACATGTATGCGAAATGCGGGAACATGAACAAGGCCATGGAAGTGTTTTGGGGGATGAAAGAAAAGAATGTGTATACATGGAGTAGTGCATTGGGAGGACTAGCCATGAATGGTTTTGGTGAGAAGTGTCTCGAGCTTTTCTCCCTTATGAATAAAGAAGGGGTTCATCCGAATGAAGTTACGTTTGTTTCGGTTCTGAGGGGTTGCACTGTGGTTGGACTAGTTGAGAAAGGTTGTCAGCATTTCGACTCAATGAGAAAATTGTACGGGATTGAGCCTCAGCTAGAGCACTACGGGTGCATGGTTGATTTATATGGTCGTGCCGGGCGTTTAGATGAAGCCCTGAGCTTCATAAACGGCATGCCAATGAAGCCTCATGCAGGTGCTTGGGGGGCTTTGCTGAACGCTAGTAGAATGTACAGGAATATGGAAATAGGTGAGCTTGCCTCGAGGAAGATAGTAGAACTGGAGGCCAAGAACCATGGAGCTTACGTGCTTTTGTCCAATATTTATGCCGATTCCAAGCTTTGGGATGGAGTTAGTAACGTGCGGCAGACTATGAAGGCAAAAGGTGTAAGGAAGCTTCCCGGTTGTAGTGTCCTAGAGGTCGATGGAGAAGTTCATGAGTTCCTTGTGGGAGACAAGTCCCATCCTCGGTACAATGAAATTGAGACAATGCTGGGAGAAATTTCTCGGCGGCTGAAGTTGGCGGGCTATGTGGCTAATACAAATCCTGTGTTGTTCGACATAGAAGAAGAAGAGAAAGAGGATGCATTGTGTAAACATAGCGAGAAGGTAGCAATTGCTTTCGGTTTGATCAGTTTGAAAGAAGGCGTGCCGATTCGGATTGTGAAGAACCTCAGGGTCTGTTGGGATTGCCATGACGTAACCAAAATGATATCTAAGCTTTTTAACAGAGAGATTGTTGTAAGAGATAGGAATAGGTTTCACCATTTTAAAGATGGTGAGTGCTCTTGCAAGGGTTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

616

Amino Acids

69.05

Weight (kDa)

8.0

Isoelectric Point (pI)

32.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	185 - 301	8.6e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	207 - 254	1.3e-09	PPR repeat family
PPR	PF01535	209 - 239	3.3e-08	PPR repeat
PPR_2	PF13041	307 - 354	2.8e-08	PPR repeat family
E_motif	PF20431	425 - 487	5.9e-21	E motif
Eplus_motif	PF20430	492 - 519	4e-07	E+ motif
DYW_deaminase	PF14432	523 - 615	2.4e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006138)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40405 AT5G40405
fragaria_vesca	FvH4_4g32050
malus_domestica	MD13G1079400.v1.1 MD16G1079200.v1.1 MD16G1079300.v1.1
prunus_persica	Prupe.1G267400_v2.0.a1
pyrus_communis	pycom13g06910 pycom16g06850
rosa_chinensis	RchiOBHm_Chr1g0381771 RchiOBHm_Chr4g0440691 RchiOBHm_Chr4g0440711
rosa_laevigata	RLG00000006158 RLG00000008805 RLG00000026207
rosa_multiflora	Rmu_sc0000463.1_g000002 Rmu_sc0008418.1_g000010
rosa_roxburghii	Rroxscaffold_5G00381500 Rroxscaffold_7G00185760
rosa_rugosa	Rorug01G0430800 Rorug04G0327600
rosa_samantha	Rh1BG411600 Rh4AG379300 Rh4BG131000 Rh4BG392100
rosa_wichuraiana	Rw1G039640 Rw4G011000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1226
AasI	GACNNNNNNGTC	1 cut(s)	440
AbsI	CCTCGAGG	1 cut(s)	1303
Acc16I	TGCGCA	1 cut(s)	657
Acc36I	ACCTGC	1 cut(s)	1226
AccB7I	CCANNNNNTGG	1 cut(s)	1383
AccI	GTMKAC	2 cut(s)	48, 929
AccII	CGCG	1 cut(s)	365
AciI	CCGC	9 cut(s)	147, 405, 450, 570, 823, 873, 1405, 1552, 1565
AclWI	GGATC	4 cut(s)	482, 614, 627, 786
AcoI	YGGCCR	1 cut(s)	133
AcsI	RAATTY	2 cut(s)	297, 1542
AcuI	CTGAAG	1 cut(s)	1577
AcvI	CACGTG	1 cut(s)	380
AdeI	CACNNNGTG	1 cut(s)	502
AfaI	GTAC	3 cut(s)	1121, 1274, 1514
AfiI	CCNNNNNNNGG	6 cut(s)	630, 768, 769, 1235, 1383, 1509
AflIII	ACRYGT	2 cut(s)	439, 858
AgeI	ACCGGT	1 cut(s)	386
AgsI	TTSAA	4 cut(s)	232, 343, 685, 1681
AhlI	ACTAGT	1 cut(s)	1075
AjnI	CCWGG	1 cut(s)	671
Alw21I	GWGCWC	4 cut(s)	8, 75, 1146, 1829
Alw26I	GTCTC	4 cut(s)	377, 989, 1488, 1520
AlwI	GGATC	4 cut(s)	482, 614, 627, 786
AlwNI	CAGNNNCTG	1 cut(s)	1722
Ama87I	CYCGRG	2 cut(s)	986, 1303
AoxI	GGCC	4 cut(s)	106, 133, 888, 1326
ApeKI	GCWGC	1 cut(s)	367
ApoI	RAATTY	2 cut(s)	297, 1542
ArsI	GACNNNNNNTTYG	2 cut(s)	566, 598
AsiGI	ACCGGT	1 cut(s)	386
Asp700I	GAANNNNTTC	1 cut(s)	1017
AspLEI	GCGC	3 cut(s)	365, 367, 658
AspS9I	GGNCC	2 cut(s)	389, 592
AsuC2I	CCSGG	2 cut(s)	1180, 1443
AsuHPI	GGTGA	9 cut(s)	116, 315, 562, 584, 844, 986, 1304, 1796, 1832
AvaI	CYCGRG	2 cut(s)	986, 1303
AvaII	GGWCC	2 cut(s)	389, 592
AxyI	CCTNAGG	1 cut(s)	1715
BamHI	GGATCC	1 cut(s)	619
BanII	GRGCYC	3 cut(s)	8, 75, 796
BarI	GAAGNNNNNNTAC	2 cut(s)	1422, 1454
BbrPI	CACGTG	1 cut(s)	380
Bbv12I	GWGCWC	4 cut(s)	8, 75, 1146, 1829
BbvCI	CCTCAGC	1 cut(s)	1134
BbvI	GCAGC	1 cut(s)	379
BccI	CCATC	7 cut(s)	176, 277, 783, 1382, 1460, 1512, 1811
BceAI	ACGGC	2 cut(s)	558, 1228
BcgI	CGANNNNNNTGC	2 cut(s)	845, 879
BciT130I	CCWGG	1 cut(s)	673
BclI	TGATCA	2 cut(s)	240, 1671
BcnI	CCSGG	2 cut(s)	1180, 1443
BcoDI	GTCTC	4 cut(s)	377, 989, 1488, 1520
BcuI	ACTAGT	1 cut(s)	1075
BfaI	CTAG	6 cut(s)	44, 956, 1076, 1139, 1263, 1457
BfuAI	ACCTGC	1 cut(s)	1226
BisI	GCNGC	4 cut(s)	147, 368, 1406, 1553
BlsI	GCNGC	4 cut(s)	148, 369, 1407, 1554
Bme1390I	CCNGG	3 cut(s)	673, 1180, 1443
Bme18I	GGWCC	2 cut(s)	389, 592
BmeT110I	CYCGRG	2 cut(s)	986, 1303
BmgT120I	GGNCC	2 cut(s)	389, 592
BmiI	GGNNCC	2 cut(s)	593, 621
BmrFI	CCNGG	3 cut(s)	673, 1180, 1443
BmsI	GCATC	4 cut(s)	5, 557, 690, 1615
BplI	GAGNNNNNCTC	6 cut(s)	778, 810, 980, 1012, 1286, 1318
BpmI	CTGGAG	1 cut(s)	1343
Bpu10I	CCTNAGC	2 cut(s)	1134, 1199
BpuEI	CTTGAG	1 cut(s)	59
BpuMI	CCSGG	2 cut(s)	1180, 1443
BsaAI	YACGTR	2 cut(s)	380, 1348
BsaI	GGTCTC	1 cut(s)	377
BsaJI	CCNNGG	5 cut(s)	400, 672, 891, 1336, 1508
BsaWI	WCCGGW	1 cut(s)	386
BsaXI	ACNNNNNCTCC	2 cut(s)	1332, 1362
Bsc4I	CCNNNNNNNGG	6 cut(s)	630, 768, 769, 1235, 1383, 1509
Bse118I	RCCGGY	1 cut(s)	386
Bse1I	ACTGG	2 cut(s)	1326, 1847
Bse21I	CCTNAGG	1 cut(s)	1715
Bse3DI	GCAATG	2 cut(s)	18, 665
BseBI	CCWGG	1 cut(s)	673
BseDI	CCNNGG	5 cut(s)	400, 672, 891, 1336, 1508
BseGI	GGATG	7 cut(s)	269, 831, 915, 1021, 1393, 1504, 1630
BseLI	CCNNNNNNNGG	6 cut(s)	630, 768, 769, 1235, 1383, 1509
BseMI	GCAATG	2 cut(s)	18, 665
BseMII	CTCAG	7 cut(s)	79, 172, 626, 1043, 1148, 1190, 1729
BseNI	ACTGG	2 cut(s)	1326, 1847
BsePI	GCGCGC	1 cut(s)	363
BseXI	GCAGC	1 cut(s)	379
BseYI	CCCAGC	2 cut(s)	838, 1534
BsgI	GTGCAG	1 cut(s)	831
Bsh1236I	CGCG	1 cut(s)	365
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	4 cut(s)	108, 135, 890, 1328
BshTI	ACCGGT	1 cut(s)	386
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	4 cut(s)	8, 75, 1146, 1829
BsiHKCI	CYCGRG	2 cut(s)	986, 1303
BsiSI	CCGG	5 cut(s)	132, 387, 524, 1179, 1443
BslFI	GGGAC	3 cut(s)	605, 856, 1486
BslI	CCNNNNNNNGG	6 cut(s)	630, 768, 769, 1235, 1383, 1509
BsmAI	GTCTC	4 cut(s)	377, 989, 1488, 1520
BsmFI	GGGAC	3 cut(s)	605, 856, 1486
BsmI	GAATGC	1 cut(s)	640
BsnI	GGCC	4 cut(s)	108, 135, 890, 1328
Bso31I	GGTCTC	1 cut(s)	377
BsoBI	CYCGRG	2 cut(s)	986, 1303
Bsp1286I	GDGCHC	5 cut(s)	8, 75, 796, 1146, 1829
Bsp1407I	TGTACA	1 cut(s)	1272
Bsp143I	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
Bsp19I	CCATGG	3 cut(s)	400, 891, 1336
BspACI	CCGC	9 cut(s)	147, 405, 450, 570, 823, 873, 1405, 1552, 1565
BspANI	GGCC	4 cut(s)	108, 135, 890, 1328
BspCNI	CTCAG	7 cut(s)	78, 171, 625, 1044, 1147, 1191, 1728
BspFNI	CGCG	1 cut(s)	365
BspHI	TCATGA	1 cut(s)	1477
BspLI	GGNNCC	2 cut(s)	593, 621
BspMI	ACCTGC	1 cut(s)	1226
BspPI	GGATC	4 cut(s)	482, 614, 627, 786
BspTNI	GGTCTC	1 cut(s)	377
BsrDI	GCAATG	2 cut(s)	18, 665
BsrFI	RCCGGY	1 cut(s)	386
BsrGI	TGTACA	1 cut(s)	1272
BsrI	ACTGG	2 cut(s)	1326, 1847
BssAI	RCCGGY	1 cut(s)	386
BssECI	CCNNGG	5 cut(s)	400, 672, 891, 1336, 1508
BssHII	GCGCGC	1 cut(s)	363
BssMI	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
BssNAI	GTATAC	1 cut(s)	930
BssT1I	CCWWGG	3 cut(s)	400, 891, 1336
Bst1107I	GTATAC	1 cut(s)	930
Bst2UI	CCWGG	1 cut(s)	673
Bst4CI	ACNGT	4 cut(s)	122, 397, 832, 1066
Bst6I	CTCTTC	2 cut(s)	227, 1608
BstAPI	GCANNNNNTGC	1 cut(s)	653
BstAUI	TGTACA	1 cut(s)	1272
BstBAI	YACGTR	2 cut(s)	380, 1348
BstC8I	GCNNGC	6 cut(s)	365, 757, 1217, 1299, 1567, 1691
BstDEI	CTNAG	9 cut(s)	11, 65, 158, 612, 1052, 1134, 1199, 1715, 1758
BstDSI	CCRYGG	3 cut(s)	400, 891, 1336
BstENI	CCTNNNNNAGG	1 cut(s)	1233
BstF5I	GGATG	7 cut(s)	269, 831, 915, 1021, 1393, 1504, 1630
BstFNI	CGCG	1 cut(s)	365
BstHHI	GCGC	3 cut(s)	365, 367, 658
BstKTI	GATC	6 cut(s)	243, 308, 490, 622, 781, 1674
BstMAI	GTCTC	4 cut(s)	377, 989, 1488, 1520
BstMBI	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	6 cut(s)	152, 635, 644, 653, 1225, 1247
BstNI	CCWGG	1 cut(s)	673
BstNSI	RCATGY	3 cut(s)	443, 862, 1219
BstSCI	CCNGG	3 cut(s)	671, 1178, 1441
BstUI	CGCG	1 cut(s)	365
BstV1I	GCAGC	1 cut(s)	379
BstX2I	RGATCY	2 cut(s)	487, 619
BstXI	CCANNNNNNTGG	1 cut(s)	175
BstYI	RGATCY	2 cut(s)	487, 619
BstZ17I	GTATAC	1 cut(s)	930
Bsu36I	CCTNAGG	1 cut(s)	1715
BsuRI	GGCC	4 cut(s)	108, 135, 890, 1328
BtgI	CCRYGG	3 cut(s)	400, 891, 1336
BtgZI	GCGATG	1 cut(s)	653
BtsCI	GGATG	7 cut(s)	269, 831, 915, 1021, 1393, 1504, 1630
BtsIMutI	CAGTG	1 cut(s)	1062
BveI	ACCTGC	1 cut(s)	1226
Cac8I	GCNNGC	6 cut(s)	365, 757, 1217, 1299, 1567, 1691
CaiI	CAGNNNCTG	1 cut(s)	1722
CciI	TCATGA	1 cut(s)	1477
CfoI	GCGC	3 cut(s)	365, 367, 658
Cfr10I	RCCGGY	1 cut(s)	386
Cfr13I	GGNCC	2 cut(s)	389, 592
CseI	GACGC	2 cut(s)	59, 844
Csp6I	GTAC	3 cut(s)	1120, 1273, 1513
CspAI	ACCGGT	1 cut(s)	386
CviQI	GTAC	3 cut(s)	1120, 1273, 1513
DdeI	CTNAG	9 cut(s)	11, 65, 158, 612, 1052, 1134, 1199, 1715, 1758
DpnI	GATC	6 cut(s)	242, 307, 489, 621, 780, 1673
DpnII	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
DraI	TTTAAA	1 cut(s)	1813
DraIII	CACNNNGTG	1 cut(s)	502
DrdI	GACNNNNNNGTC	1 cut(s)	440
DseDI	GACNNNNNNGTC	1 cut(s)	440
EaeI	YGGCCR	1 cut(s)	133
Eam1104I	CTCTTC	2 cut(s)	227, 1608
EarI	CTCTTC	2 cut(s)	227, 1608
EciI	GGCGGA	1 cut(s)	420
Ecl136II	GAGCTC	2 cut(s)	6, 73
Eco130I	CCWWGG	3 cut(s)	400, 891, 1336
Eco24I	GRGCYC	3 cut(s)	8, 75, 796
Eco31I	GGTCTC	1 cut(s)	377
Eco32I	GATATC	1 cut(s)	1755
Eco47I	GGWCC	2 cut(s)	389, 592
Eco53kI	GAGCTC	2 cut(s)	6, 73
Eco57I	CTGAAG	1 cut(s)	1577
Eco72I	CACGTG	1 cut(s)	380
Eco81I	CCTNAGG	1 cut(s)	1715
Eco88I	CYCGRG	2 cut(s)	986, 1303
EcoICRI	GAGCTC	2 cut(s)	6, 73
EcoNI	CCTNNNNNAGG	1 cut(s)	1233
EcoO109I	RGGNCCY	1 cut(s)	592
EcoRI	GAATTC	1 cut(s)	297
EcoRII	CCWGG	1 cut(s)	671
EcoRV	GATATC	1 cut(s)	1755
EcoT14I	CCWWGG	3 cut(s)	400, 891, 1336
EcoT22I	ATGCAT	2 cut(s)	20, 1630
EcoT38I	GRGCYC	3 cut(s)	8, 75, 796
ErhI	CCWWGG	3 cut(s)	400, 891, 1336
FaqI	GGGAC	3 cut(s)	605, 856, 1486
FauI	CCCGC	3 cut(s)	443, 866, 1558
FbaI	TGATCA	2 cut(s)	240, 1671
FblI	GTMKAC	2 cut(s)	48, 929
Fnu4HI	GCNGC	4 cut(s)	147, 368, 1406, 1553
FokI	GGATG	7 cut(s)	256, 838, 922, 1008, 1400, 1491, 1637
FriOI	GRGCYC	3 cut(s)	8, 75, 796
Fsp4HI	GCNGC	4 cut(s)	147, 368, 1406, 1553
FspBI	CTAG	6 cut(s)	44, 956, 1076, 1139, 1263, 1457
FspI	TGCGCA	1 cut(s)	657
GlaI	GCGC	3 cut(s)	364, 366, 657
GluI	GCNGC	4 cut(s)	147, 368, 1406, 1553
GsaI	CCCAGC	2 cut(s)	842, 1538
GsuI	CTGGAG	1 cut(s)	1343
HaeIII	GGCC	4 cut(s)	108, 135, 890, 1328
HapII	CCGG	5 cut(s)	132, 387, 524, 1179, 1443
HgaI	GACGC	2 cut(s)	59, 844
HhaI	GCGC	3 cut(s)	365, 367, 658
Hin6I	GCGC	3 cut(s)	363, 365, 656
HinP1I	GCGC	3 cut(s)	363, 365, 656
HindIII	AAGCTT	3 cut(s)	1379, 1436, 1760
HinfI	GANTC	4 cut(s)	685, 1103, 1373, 1696
HpaII	CCGG	5 cut(s)	132, 387, 524, 1179, 1443
HphI	GGTGA	9 cut(s)	116, 315, 562, 584, 844, 986, 1304, 1796, 1832
Hpy166II	GTNNAC	3 cut(s)	49, 930, 1637
Hpy188I	TCNGA	8 cut(s)	68, 161, 245, 305, 615, 1026, 1053, 1701
Hpy188III	TCNNGA	2 cut(s)	986, 1478
Hpy8I	GTNNAC	3 cut(s)	49, 930, 1637
HpyAV	CCTTC	7 cut(s)	119, 238, 349, 1007, 1411, 1642, 1679
HpyCH4III	ACNGT	4 cut(s)	122, 397, 832, 1066
HpyCH4IV	ACGT	6 cut(s)	379, 576, 1037, 1347, 1401, 1740
HpyF10VI	GCNNNNNNNGC	6 cut(s)	152, 635, 644, 653, 1225, 1247
HpyF3I	CTNAG	9 cut(s)	11, 65, 158, 612, 1052, 1134, 1199, 1715, 1758
HpySE526I	ACGT	6 cut(s)	379, 576, 1037, 1347, 1401, 1740
HspAI	GCGC	3 cut(s)	363, 365, 656
Ksp22I	TGATCA	2 cut(s)	240, 1671
Kzo9I	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
LmnI	GCTCC	4 cut(s)	11, 266, 764, 1340
Lsp1109I	GCAGC	1 cut(s)	379
LweI	GCATC	4 cut(s)	5, 557, 690, 1615
MaeI	CTAG	6 cut(s)	44, 956, 1076, 1139, 1263, 1457
MaeII	ACGT	6 cut(s)	379, 576, 1037, 1347, 1401, 1740
MaeIII	GTNAC	9 cut(s)	122, 311, 494, 572, 832, 1033, 1397, 1741, 1838
MalI	GATC	6 cut(s)	242, 307, 489, 621, 780, 1673
MboI	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
MboII	GAAGA	7 cut(s)	214, 823, 1321, 1619, 1622, 1625, 1720
MfeI	CAATTG	2 cut(s)	36, 1656
MflI	RGATCY	2 cut(s)	487, 619
MhlI	GDGCHC	5 cut(s)	8, 75, 796, 1146, 1829
MlyI	GAGTC	1 cut(s)	1097
MmeI	TCCRAC	1 cut(s)	1051
Mph1103I	ATGCAT	2 cut(s)	20, 1630
MroXI	GAANNNNTTC	1 cut(s)	1017
MseI	TTAA	4 cut(s)	102, 455, 1767, 1812
MslI	CAYNNNNRTG	5 cut(s)	857, 896, 1026, 1220, 1236
MspI	CCGG	5 cut(s)	132, 387, 524, 1179, 1443
MspR9I	CCNGG	3 cut(s)	673, 1180, 1443
MunI	CAATTG	2 cut(s)	36, 1656
Mva1269I	GAATGC	1 cut(s)	640
MvaI	CCWGG	1 cut(s)	673
MvnI	CGCG	1 cut(s)	365
MwoI	GCNNNNNNNGC	6 cut(s)	152, 635, 644, 653, 1225, 1247
NciI	CCSGG	2 cut(s)	1180, 1443
NcoI	CCATGG	3 cut(s)	400, 891, 1336
NdeII	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
NlaIV	GGNNCC	2 cut(s)	593, 621
NmeAIII	GCCGAG	1 cut(s)	1528
NmuCI	GTSAC	5 cut(s)	122, 311, 494, 572, 832
NsbI	TGCGCA	1 cut(s)	657
NsiI	ATGCAT	2 cut(s)	20, 1630
NspI	RCATGY	3 cut(s)	443, 862, 1219
PaeI	GCATGC	1 cut(s)	1219
PaeR7I	CTCGAG	2 cut(s)	986, 1303
PagI	TCATGA	1 cut(s)	1477
PaqCI	CACCTGC	1 cut(s)	1226
PauI	GCGCGC	1 cut(s)	363
PciI	ACATGT	2 cut(s)	439, 858
PcsI	WCGNNNNNNNCGW	1 cut(s)	1180
PctI	GAATGC	1 cut(s)	640
PdmI	GAANNNNTTC	1 cut(s)	1017
PfeI	GAWTC	3 cut(s)	685, 1373, 1696
PflMI	CCANNNNNTGG	1 cut(s)	1383
PinAI	ACCGGT	1 cut(s)	386
PkrI	GCNGC	4 cut(s)	148, 369, 1407, 1554
PleI	GAGTC	1 cut(s)	1097
PmaCI	CACGTG	1 cut(s)	380
PmlI	CACGTG	1 cut(s)	380
PpsI	GAGTC	1 cut(s)	1097
Ppu21I	YACGTR	2 cut(s)	380, 1348
PpuMI	RGGWCCY	1 cut(s)	592
PscI	ACATGT	2 cut(s)	439, 858
Psp124BI	GAGCTC	2 cut(s)	8, 75
Psp5II	RGGWCCY	1 cut(s)	592
Psp6I	CCWGG	1 cut(s)	671
PspCI	CACGTG	1 cut(s)	380
PspFI	CCCAGC	2 cut(s)	838, 1534
PspGI	CCWGG	1 cut(s)	671
PspN4I	GGNNCC	2 cut(s)	593, 621
PspPI	GGNCC	2 cut(s)	389, 592
PspPPI	RGGWCCY	1 cut(s)	592
PspXI	VCTCGAGB	1 cut(s)	1303
PstNI	CAGNNNCTG	1 cut(s)	1722
PsuI	RGATCY	2 cut(s)	487, 619
PteI	GCGCGC	1 cut(s)	363
RsaI	GTAC	3 cut(s)	1121, 1274, 1514
RsaNI	GTAC	3 cut(s)	1120, 1273, 1513
RseI	CAYNNNNRTG	5 cut(s)	857, 896, 1026, 1220, 1236
SacI	GAGCTC	2 cut(s)	8, 75
SaqAI	TTAA	4 cut(s)	102, 455, 1767, 1812
SatI	GCNGC	4 cut(s)	147, 368, 1406, 1553
Sau3AI	GATC	6 cut(s)	240, 305, 487, 619, 778, 1671
Sau96I	GGNCC	2 cut(s)	389, 592
SchI	GAGTC	1 cut(s)	1097
ScrFI	CCNGG	3 cut(s)	673, 1180, 1443
SduI	GDGCHC	5 cut(s)	8, 75, 796, 1146, 1829
SfaNI	GCATC	4 cut(s)	5, 557, 690, 1615
Sfr274I	CTCGAG	2 cut(s)	986, 1303
SinI	GGWCC	2 cut(s)	389, 592
SlaI	CTCGAG	2 cut(s)	986, 1303
SmiMI	CAYNNNNRTG	5 cut(s)	857, 896, 1026, 1220, 1236
SmlI	CTYRAG	3 cut(s)	74, 986, 1303
SmoI	CTYRAG	3 cut(s)	74, 986, 1303
SpeI	ACTAGT	1 cut(s)	1075
SphI	GCATGC	1 cut(s)	1219
SsiI	CCGC	9 cut(s)	147, 405, 450, 570, 823, 873, 1405, 1552, 1565
SspI	AATATT	1 cut(s)	1363
SspMI	CTAG	6 cut(s)	44, 956, 1076, 1139, 1263, 1457
SstI	GAGCTC	2 cut(s)	8, 75
StyD4I	CCNGG	3 cut(s)	671, 1178, 1441
StyI	CCWWGG	3 cut(s)	400, 891, 1336
TaaI	ACNGT	4 cut(s)	122, 397, 832, 1066
TaiI	ACGT	6 cut(s)	382, 579, 1040, 1350, 1404, 1743
TaqI	TCGA	6 cut(s)	855, 987, 1101, 1304, 1464, 1599
TatI	WGTACW	1 cut(s)	1272
TauI	GCSGC	3 cut(s)	149, 1408, 1555
TfiI	GAWTC	3 cut(s)	685, 1373, 1696
Tru1I	TTAA	4 cut(s)	102, 455, 1767, 1812
Tru9I	TTAA	4 cut(s)	102, 455, 1767, 1812
TscAI	CASTG	1 cut(s)	1069
TseFI	GTSAC	5 cut(s)	122, 311, 494, 572, 832
TseI	GCWGC	1 cut(s)	367
Tsp45I	GTSAC	5 cut(s)	122, 311, 494, 572, 832
TspGWI	ACGGA	1 cut(s)	480
TspRI	CASTG	1 cut(s)	1069
Van91I	CCANNNNNTGG	1 cut(s)	1383
VpaK11BI	GGWCC	2 cut(s)	389, 592
XagI	CCTNNNNNAGG	1 cut(s)	1233
XapI	RAATTY	2 cut(s)	297, 1542
XceI	RCATGY	3 cut(s)	443, 862, 1219
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1814
XhoI	CTCGAG	2 cut(s)	986, 1303
XmiI	GTMKAC	2 cut(s)	48, 929
XmnI	GAANNNNTTC	1 cut(s)	1017
XspI	CTAG	6 cut(s)	44, 956, 1076, 1139, 1263, 1457
Zsp2I	ATGCAT	2 cut(s)	20, 1630

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.