Rw1G039640

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006138
Physical Location & Seq
Forward (+)
67153316 .. 67155166
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G039640.1

Sequence Viewer

Length: 1851 bp
ATGAGCTCTTTGAGATGCATTGCAAAACACCCAACAATTGCACTAGTAGACTCATCCACCACTCTCAAAGATCTCAAGCAAATCCACACCCAACTCCTCATTAACGGCGTTCTCAACGATCCTAACCTCTCCGGCAATTTTGTTGCCACCATTGCTGTCCGAAACCCCAACAATTTGGAATATTCCAACCGAGTCCTGGACCAATGTGACAACCCATCTCTCTTTGCGTTCAACTCCATGATCAGGGCCTATTCGAAAAGCTCCACACCCAGTAAAAGCTTTCACTTTTACAGCAGAATCCTCCATTCATGTAACAATCTTCTACCTGATAACTACACATTTAACTTCTTGGTTCGCACTTCTGCGCAGCTTTCGTCATTTGAGACTGGTCCTTCGGTCCATGGTGCATTAGTAAAGCATGGATTTGAGAATGACCCGCATGTTCAAAGTGCTTTAATATTTATGTATGCGGAATTGGGTTGCTTGGAGCAATGTCATAGAGTGTTTAGCGGCATTGCCGATCCTGATTTGGTGTGTCAGACTGCTATTGTGAGCGCTTGTGCTAAATGTGGGGATGTAGATTATGCACGGTTATTGTTCGATGAAATGCCTGAGAGAGACCCCATTGCTTGGAATGCGATGATTGCGGGGTATGCGCAATGTGGGAAGTCTAGGGAAGCTTTGGGTTTGTTCCATTTGATGCAAATGGAGGGTGTGAGGGTGAATGAGGTGTGTATGGTTTCGGTGTTATCGGCGTGTTGTCACTTGGGGGCACTGGATCAAGGGAGATGGGCTCATGCATATATAGAGAGAAACAAGGTGAGGATGACAGTGACATTGGGGACTGCACTGCTTGACATGTATGCGAAATGTGGGAACATGAGTAAGGCTATGGAAGTGTTTTGGGGGATGAAGGAAAAGAATGTGTATACGTGGAGTGTTGCCTTGGGGGGACTAGCTATGAATGGCTTTGGTGAGAAGTGCCTTGAGCTTTTCTCAGTCATGGAGAAAGAAGGCGTTCGGCCAAATGAAGTTACCTTTGTTTCGGTTCTTAGGGGTTGTACTGTGGTTGGACTAGTGGAGGAAGGTCGTCGACATTTTGACTCGATGAAAGAATTGTATGGGATTGAACCTCAGCTCGAGCATTATGGGTGCATTGTTGACTTGTATGGTCGAGCTGGGCGTTTAGATGAAGCCTTGAACTTCATTAACAACATGCCAATGAAGCCTCATGCGGGTGCTTGGGGGGCTTTGCTCCACGCTAGTAGAATGTATCGGAATATGGAACTGGGTGAGCTTGCGTCGAGAAAGATAGTAGAACTCGAGGACAGGAATCACGGTGCTTATGTGCTTTTGTCCAATATATATGCGGATTCCAAGCTTTATGATGGAGTTAGTAATGTGCGGCAAAATATGAAGGCTAAAGGTGTGAGGAAGCTTCCTGGTTGTAGTGTCATAGAAGTTGATGGGGAAGTTCATGAATTCTTTGCAGGGGAAGACAATTCTCATCCAAGGTATAATGAAATCGAAATGATGCTGGAAGAAATCTCCCGGCGCCTGAAATTAGCAGGTTATGTTGCCAATACCAACCCTGTGCTGTTCGATATAGAAGAAGAAGAGAAAGAGGATGCATTGTTTAAACACAGCGAGAAGTTTGCCATTGCTTTTGGTTTGATCGGTTTGAGAGAAGGAGTGCCCATTAGGATTGCAAAGAACCTCAGGGTCTGTTGGGATTGTCATGATGTAACAAAAATGATATCCAAGATTTTCAATAGAGTGATTATAGTAAGAGACAGAAATAGGTTTCACCATTTTCAAAATGGTGAGTGCTCTTGTAAGGATTATTGGTGA

Protein Analysis

616

Amino Acids

69.33

Weight (kDa)

6.35

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 207 - 255 1.7e-09 PPR repeat family
PPR PF01535 210 - 239 5.3e-08 PPR repeat
PPR_2 PF13041 307 - 354 1.4e-08 PPR repeat family
E_motif PF20431 425 - 487 1.4e-19 E motif
DYW_deaminase PF14432 524 - 616 8.3e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 366, 657
Acc36I ACCTGC 1 cut(s) 1559
AccB1I GGYRCC 1 cut(s) 1554
AccB7I CCANNNNNTGG 1 cut(s) 630
AccI GTMKAC 3 cut(s) 48, 929, 1093
AciI CCGC 7 cut(s) 437, 470, 510, 647, 1235, 1370, 1405
AclWI GGATC 3 cut(s) 113, 515, 786
AcoI YGGCCR 1 cut(s) 1022
AcsI RAATTY 1 cut(s) 1481
AcyI GRCGYC 1 cut(s) 1555
AfaI GTAC 1 cut(s) 1063
AfeI AGCGCT 1 cut(s) 556
AfiI CCNNNNNNNGG 4 cut(s) 196, 243, 630, 1235
AflIII ACRYGT 1 cut(s) 858
AgsI TTSAA 6 cut(s) 232, 446, 1130, 1201, 1771, 1817
AhlI ACTAGT 2 cut(s) 43, 1075
AjnI CCWGG 2 cut(s) 195, 1441
Alw21I GWGCWC 2 cut(s) 8, 1832
Alw26I GTCTC 3 cut(s) 377, 612, 1785
AlwI GGATC 3 cut(s) 113, 515, 786
AlwNI CAGNNNCTG 1 cut(s) 1725
Ama87I CYCGRG 2 cut(s) 1139, 1322
Aor51HI AGCGCT 1 cut(s) 556
AoxI GGCC 2 cut(s) 246, 1022
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 1481
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
Asp700I GAANNNNTTC 1 cut(s) 1017
AspLEI GCGC 4 cut(s) 367, 557, 658, 1557
AspS9I GGNCC 4 cut(s) 199, 246, 389, 397
AsuC2I CCSGG 1 cut(s) 1552
AsuHPI GGTGA 6 cut(s) 733, 832, 986, 1304, 1799, 1835
AsuII TTCGAA 1 cut(s) 254
AvaI CYCGRG 2 cut(s) 1139, 1322
AvaII GGWCC 3 cut(s) 199, 389, 397
AxyI CCTNAGG 1 cut(s) 1718
BaeGI GKGCMC 2 cut(s) 775, 1698
BanI GGYRCC 1 cut(s) 1554
BanII GRGCYC 2 cut(s) 8, 796
BbsI GAAGAC 1 cut(s) 1503
Bbv12I GWGCWC 2 cut(s) 8, 1832
BbvCI CCTCAGC 1 cut(s) 1134
BbvI GCAGC 1 cut(s) 379
BccI CCATC 4 cut(s) 223, 783, 1382, 1460
BceAI ACGGC 1 cut(s) 121
BcgI CGANNNNNNTGC 2 cut(s) 1637, 1671
BciT130I CCWGG 2 cut(s) 197, 1443
BclI TGATCA 1 cut(s) 240
BcnI CCSGG 1 cut(s) 1552
BcoDI GTCTC 3 cut(s) 377, 612, 1785
BcuI ACTAGT 2 cut(s) 43, 1075
BfaI CTAG 5 cut(s) 44, 672, 956, 1076, 1263
BfoI RGCGCY 2 cut(s) 558, 1558
BfuAI ACCTGC 1 cut(s) 1559
BglII AGATCT 1 cut(s) 70
BisI GCNGC 3 cut(s) 368, 511, 1406
BlsI GCNGC 3 cut(s) 369, 512, 1407
Bme1390I CCNGG 3 cut(s) 197, 1443, 1552
Bme18I GGWCC 3 cut(s) 199, 389, 397
BmeT110I CYCGRG 2 cut(s) 1139, 1322
BmgT120I GGNCC 4 cut(s) 199, 246, 389, 397
BmiI GGNNCC 1 cut(s) 1556
BmrFI CCNGG 3 cut(s) 197, 1443, 1552
BmrI ACTGGG 2 cut(s) 264, 1298
BmsI GCATC 4 cut(s) 5, 690, 1524, 1618
BmuI ACTGGG 2 cut(s) 264, 1298
BpiI GAAGAC 1 cut(s) 1503
BplI GAGNNNNNCTC 4 cut(s) 778, 810, 980, 1012
Bpu10I CCTNAGC 1 cut(s) 1134
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 2 cut(s) 59, 1007
BpuMI CCSGG 1 cut(s) 1552
BsaAI YACGTR 1 cut(s) 933
BsaHI GRCGYC 1 cut(s) 1555
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 3 cut(s) 400, 945, 1511
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 243, 630, 1235
Bse1I ACTGG 4 cut(s) 270, 391, 780, 1293
Bse21I CCTNAGG 1 cut(s) 1718
Bse3DI GCAATG 7 cut(s) 18, 150, 497, 513, 624, 665, 1659
BseBI CCWGG 2 cut(s) 197, 1443
BseDI CCNNGG 3 cut(s) 400, 945, 1511
BseGI GGATG 6 cut(s) 53, 580, 831, 915, 1507, 1633
BseLI CCNNNNNNNGG 4 cut(s) 196, 243, 630, 1235
BseMI GCAATG 7 cut(s) 18, 150, 497, 513, 624, 665, 1659
BseMII CTCAG 4 cut(s) 603, 1011, 1148, 1732
BseNI ACTGG 4 cut(s) 270, 391, 780, 1293
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 2 cut(s) 775, 1698
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 1178
BsgI GTGCAG 1 cut(s) 831
BshFI GGCC 2 cut(s) 248, 1024
BshNI GGYRCC 1 cut(s) 1554
BsiHKAI GWGCWC 2 cut(s) 8, 1832
BsiHKCI CYCGRG 2 cut(s) 1139, 1322
BsiSI CCGG 2 cut(s) 132, 1552
BslFI GGGAC 2 cut(s) 856, 966
BslI CCNNNNNNNGG 4 cut(s) 196, 243, 630, 1235
BsmAI GTCTC 3 cut(s) 377, 612, 1785
BsmFI GGGAC 2 cut(s) 856, 966
BsmI GAATGC 1 cut(s) 640
BsnI GGCC 2 cut(s) 248, 1024
Bso31I GGTCTC 1 cut(s) 612
BsoBI CYCGRG 2 cut(s) 1139, 1322
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 5 cut(s) 8, 775, 796, 1698, 1832
Bsp143I GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
Bsp19I CCATGG 1 cut(s) 400
BspACI CCGC 7 cut(s) 437, 470, 510, 647, 1235, 1370, 1405
BspANI GGCC 2 cut(s) 248, 1024
BspCNI CTCAG 4 cut(s) 604, 1010, 1147, 1731
BspHI TCATGA 2 cut(s) 1477, 1738
BspLI GGNNCC 1 cut(s) 1556
BspMI ACCTGC 1 cut(s) 1559
BspPI GGATC 3 cut(s) 113, 515, 786
BspT104I TTCGAA 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 1554
BspTNI GGTCTC 1 cut(s) 612
BsrDI GCAATG 7 cut(s) 18, 150, 497, 513, 624, 665, 1659
BsrI ACTGG 4 cut(s) 270, 391, 780, 1293
BssECI CCNNGG 3 cut(s) 400, 945, 1511
BssMI GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
BssNAI GTATAC 1 cut(s) 930
BssNI GRCGYC 1 cut(s) 1555
BssT1I CCWWGG 3 cut(s) 400, 945, 1511
Bst1107I GTATAC 1 cut(s) 930
Bst2UI CCWGG 2 cut(s) 197, 1443
Bst4CI ACNGT 4 cut(s) 591, 832, 1066, 1340
Bst6I CTCTTC 1 cut(s) 1611
BstACI GRCGYC 1 cut(s) 1555
BstBAI YACGTR 1 cut(s) 933
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 1299
BstDEI CTNAG 5 cut(s) 612, 997, 1052, 1134, 1718
BstDSI CCRYGG 1 cut(s) 400
BstF5I GGATG 6 cut(s) 53, 580, 831, 915, 1507, 1633
BstH2I RGCGCY 2 cut(s) 558, 1558
BstHHI GCGC 4 cut(s) 367, 557, 658, 1557
BstKTI GATC 6 cut(s) 73, 121, 243, 523, 781, 1677
BstMAI GTCTC 3 cut(s) 377, 612, 1785
BstMBI GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
BstMWI GCNNNNNNNGC 6 cut(s) 152, 635, 644, 653, 1225, 1247
BstNI CCWGG 2 cut(s) 197, 1443
BstNSI RCATGY 3 cut(s) 443, 862, 1219
BstSCI CCNGG 3 cut(s) 195, 1441, 1550
BstSLI GKGCMC 2 cut(s) 775, 1698
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 1503
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 1 cut(s) 70
BstZ17I GTATAC 1 cut(s) 930
Bsu36I CCTNAGG 1 cut(s) 1718
BsuRI GGCC 2 cut(s) 248, 1024
BtgI CCRYGG 1 cut(s) 400
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 6 cut(s) 53, 580, 831, 915, 1507, 1633
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 3 cut(s) 773, 837, 848
BveI ACCTGC 1 cut(s) 1559
Cac8I GCNNGC 1 cut(s) 1299
CaiI CAGNNNCTG 1 cut(s) 1725
CciI TCATGA 2 cut(s) 1477, 1738
CfoI GCGC 4 cut(s) 367, 557, 658, 1557
Cfr13I GGNCC 4 cut(s) 199, 246, 389, 397
CseI GACGC 1 cut(s) 1290
Csp6I GTAC 1 cut(s) 1062
CviQI GTAC 1 cut(s) 1062
DdeI CTNAG 5 cut(s) 612, 997, 1052, 1134, 1718
DinI GGCGCC 1 cut(s) 1556
DpnI GATC 6 cut(s) 72, 120, 242, 522, 780, 1676
DpnII GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
DraI TTTAAA 1 cut(s) 1639
EaeI YGGCCR 1 cut(s) 1022
Eam1104I CTCTTC 1 cut(s) 1611
EarI CTCTTC 1 cut(s) 1611
Ecl136II GAGCTC 1 cut(s) 6
Eco130I CCWWGG 3 cut(s) 400, 945, 1511
Eco24I GRGCYC 2 cut(s) 8, 796
Eco31I GGTCTC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 1758
Eco47I GGWCC 3 cut(s) 199, 389, 397
Eco47III AGCGCT 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 6
Eco81I CCTNAGG 1 cut(s) 1718
Eco88I CYCGRG 2 cut(s) 1139, 1322
EcoICRI GAGCTC 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 1481
EcoRII CCWGG 2 cut(s) 195, 1441
EcoRV GATATC 1 cut(s) 1758
EcoT14I CCWWGG 3 cut(s) 400, 945, 1511
EcoT22I ATGCAT 3 cut(s) 20, 802, 1633
EcoT38I GRGCYC 2 cut(s) 8, 796
EgeI GGCGCC 1 cut(s) 1556
EheI GGCGCC 1 cut(s) 1556
ErhI CCWWGG 3 cut(s) 400, 945, 1511
FaqI GGGAC 2 cut(s) 856, 966
FauI CCCGC 3 cut(s) 444, 640, 1228
FbaI TGATCA 1 cut(s) 240
FblI GTMKAC 3 cut(s) 48, 929, 1093
Fnu4HI GCNGC 3 cut(s) 368, 511, 1406
FokI GGATG 6 cut(s) 40, 587, 838, 922, 1494, 1640
FriOI GRGCYC 2 cut(s) 8, 796
Fsp4HI GCNGC 3 cut(s) 368, 511, 1406
FspBI CTAG 5 cut(s) 44, 672, 956, 1076, 1263
FspI TGCGCA 2 cut(s) 366, 657
GlaI GCGC 4 cut(s) 366, 556, 657, 1556
GluI GCNGC 3 cut(s) 368, 511, 1406
GsaI CCCAGC 1 cut(s) 1182
HaeII RGCGCY 2 cut(s) 558, 1558
HaeIII GGCC 2 cut(s) 248, 1024
HapII CCGG 2 cut(s) 132, 1552
HgaI GACGC 1 cut(s) 1290
HhaI GCGC 4 cut(s) 367, 557, 658, 1557
Hin1I GRCGYC 1 cut(s) 1555
Hin6I GCGC 4 cut(s) 365, 555, 656, 1555
HinP1I GCGC 4 cut(s) 365, 555, 656, 1555
HincII GTYRAC 2 cut(s) 1094, 1162
HindII GTYRAC 2 cut(s) 1094, 1162
HindIII AAGCTT 4 cut(s) 277, 678, 1379, 1436
HinfI GANTC 6 cut(s) 50, 192, 297, 1103, 1333, 1373
HpaII CCGG 2 cut(s) 132, 1552
HphI GGTGA 6 cut(s) 733, 832, 986, 1304, 1799, 1835
Hpy166II GTNNAC 4 cut(s) 49, 930, 1094, 1162
Hpy188I TCNGA 3 cut(s) 161, 540, 1278
Hpy188III TCNNGA 4 cut(s) 524, 1305, 1478, 1739
Hpy8I GTNNAC 4 cut(s) 49, 930, 1094, 1162
Hpy99I CGWCG 2 cut(s) 1095, 1306
HpyAV CCTTC 6 cut(s) 402, 907, 1007, 1079, 1411, 1682
HpyCH4III ACNGT 4 cut(s) 591, 832, 1066, 1340
HpyCH4IV ACGT 1 cut(s) 932
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 635, 644, 653, 1225, 1247
HpyF3I CTNAG 5 cut(s) 612, 997, 1052, 1134, 1718
HpySE526I ACGT 1 cut(s) 932
Hsp92I GRCGYC 1 cut(s) 1555
HspAI GCGC 4 cut(s) 365, 555, 656, 1555
KasI GGCGCC 1 cut(s) 1554
Ksp22I TGATCA 1 cut(s) 240
Kzo9I GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
LmnI GCTCC 3 cut(s) 266, 487, 1260
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 4 cut(s) 5, 690, 1524, 1618
MaeI CTAG 5 cut(s) 44, 672, 956, 1076, 1263
MaeII ACGT 1 cut(s) 932
MaeIII GTNAC 6 cut(s) 206, 311, 761, 832, 1033, 1744
MalI GATC 6 cut(s) 72, 120, 242, 522, 780, 1676
MboI GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
MboII GAAGA 6 cut(s) 311, 1508, 1553, 1622, 1625, 1628
MfeI CAATTG 1 cut(s) 36
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 5 cut(s) 8, 775, 796, 1698, 1832
MluCI AATT 8 cut(s) 36, 136, 172, 473, 1115, 1481, 1501, 1562
Mly113I GGCGCC 1 cut(s) 1555
MlyI GAGTC 3 cut(s) 44, 201, 1097
MmeI TCCRAC 2 cut(s) 210, 1051
Mph1103I ATGCAT 3 cut(s) 20, 802, 1633
MroXI GAANNNNTTC 1 cut(s) 1017
MseI TTAA 5 cut(s) 102, 342, 455, 1209, 1638
MslI CAYNNNNRTG 2 cut(s) 1220, 1236
MspI CCGG 2 cut(s) 132, 1552
MspR9I CCNGG 3 cut(s) 197, 1443, 1552
MssI GTTTAAAC 1 cut(s) 1639
MunI CAATTG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 640
MvaI CCWGG 2 cut(s) 197, 1443
MwoI GCNNNNNNNGC 6 cut(s) 152, 635, 644, 653, 1225, 1247
NarI GGCGCC 1 cut(s) 1555
NciI CCSGG 1 cut(s) 1552
NcoI CCATGG 1 cut(s) 400
NdeII GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
NlaIV GGNNCC 1 cut(s) 1556
NmuCI GTSAC 3 cut(s) 206, 761, 832
NsbI TGCGCA 2 cut(s) 366, 657
NsiI ATGCAT 3 cut(s) 20, 802, 1633
NspI RCATGY 3 cut(s) 443, 862, 1219
NspV TTCGAA 1 cut(s) 254
PaeR7I CTCGAG 2 cut(s) 1139, 1322
PagI TCATGA 2 cut(s) 1477, 1738
PciI ACATGT 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 1180
PctI GAATGC 1 cut(s) 640
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 3 cut(s) 297, 1333, 1373
PflMI CCANNNNNTGG 1 cut(s) 630
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 3 cut(s) 369, 512, 1407
PleI GAGTC 3 cut(s) 44, 200, 1097
PluTI GGCGCC 1 cut(s) 1558
PmeI GTTTAAAC 1 cut(s) 1639
PpsI GAGTC 3 cut(s) 44, 200, 1097
Ppu21I YACGTR 1 cut(s) 933
PscI ACATGT 1 cut(s) 858
Psp124BI GAGCTC 1 cut(s) 8
Psp6I CCWGG 2 cut(s) 195, 1441
PspFI CCCAGC 1 cut(s) 1178
PspGI CCWGG 2 cut(s) 195, 1441
PspN4I GGNNCC 1 cut(s) 1556
PspPI GGNCC 4 cut(s) 199, 246, 389, 397
PspXI VCTCGAGB 2 cut(s) 1139, 1322
PstNI CAGNNNCTG 1 cut(s) 1725
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 1063
RsaNI GTAC 1 cut(s) 1062
RseI CAYNNNNRTG 2 cut(s) 1220, 1236
SacI GAGCTC 1 cut(s) 8
SalI GTCGAC 1 cut(s) 1092
SaqAI TTAA 5 cut(s) 102, 342, 455, 1209, 1638
SatI GCNGC 3 cut(s) 368, 511, 1406
Sau3AI GATC 6 cut(s) 70, 118, 240, 520, 778, 1674
Sau96I GGNCC 4 cut(s) 199, 246, 389, 397
SchI GAGTC 3 cut(s) 44, 201, 1097
ScrFI CCNGG 3 cut(s) 197, 1443, 1552
SduI GDGCHC 5 cut(s) 8, 775, 796, 1698, 1832
SfaNI GCATC 4 cut(s) 5, 690, 1524, 1618
SfoI GGCGCC 1 cut(s) 1556
Sfr274I CTCGAG 2 cut(s) 1139, 1322
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 3 cut(s) 199, 389, 397
SlaI CTCGAG 2 cut(s) 1139, 1322
SmiMI CAYNNNNRTG 2 cut(s) 1220, 1236
SmlI CTYRAG 4 cut(s) 74, 986, 1139, 1322
SmoI CTYRAG 4 cut(s) 74, 986, 1139, 1322
SpeI ACTAGT 2 cut(s) 43, 1075
Sse9I AATT 8 cut(s) 36, 136, 172, 473, 1115, 1481, 1501, 1562
SsiI CCGC 7 cut(s) 437, 470, 510, 647, 1235, 1370, 1405
SspDI GGCGCC 1 cut(s) 1554
SspI AATATT 2 cut(s) 182, 459
SspMI CTAG 5 cut(s) 44, 672, 956, 1076, 1263
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 3 cut(s) 195, 1441, 1550
StyI CCWWGG 3 cut(s) 400, 945, 1511
TaaI ACNGT 4 cut(s) 591, 832, 1066, 1340
TaiI ACGT 1 cut(s) 935
TaqII GACCGA 1 cut(s) 385
TasI AATT 8 cut(s) 36, 136, 172, 473, 1115, 1481, 1501, 1562
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 2 cut(s) 513, 1408
TfiI GAWTC 3 cut(s) 297, 1333, 1373
Tru1I TTAA 5 cut(s) 102, 342, 455, 1209, 1638
Tru9I TTAA 5 cut(s) 102, 342, 455, 1209, 1638
TscAI CASTG 3 cut(s) 780, 837, 855
TseFI GTSAC 3 cut(s) 206, 761, 832
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 3 cut(s) 206, 761, 832
TspRI CASTG 3 cut(s) 780, 837, 855
Van91I CCANNNNNTGG 1 cut(s) 630
VpaK11BI GGWCC 3 cut(s) 199, 389, 397
XapI RAATTY 1 cut(s) 1481
XceI RCATGY 3 cut(s) 443, 862, 1219
XcmI CCANNNNNNNNNTGG 2 cut(s) 193, 1817
XhoI CTCGAG 2 cut(s) 1139, 1322
XmiI GTMKAC 3 cut(s) 48, 929, 1093
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 5 cut(s) 44, 672, 956, 1076, 1263
Zsp2I ATGCAT 3 cut(s) 20, 802, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.