RLG00000026207

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006138
Physical Location & Seq
Reverse (-)
1235750 .. 1237810
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026207

Sequence Viewer

Length: 1305 bp
ATGTATGCGGAATTGGGTTGCTTGGAGCAATGTCATAGAGTGTTTAGCGGCATTGCCGATCCTGATTTGGTGTGTCAGACTGCTATTGTGAGCGCTTGTGCTAAATGTGGGGATGTAGATTATGCACGGTTATTGTTCGATGAAATGCCTGAGAGAGACCCCATTTCTTGGAATGCGATGATTGCGGGGAATGCGCAATGTGGGAAGTCTAGGGAAGCTTTGGGTTTGTTCCATTTGATGCAAATGGAGGGTGTGAGGGTGAACGAGGTGTGTATGGTTTCGGTGTTATCAGCGTGTTGTCACTTGGGGGCACTGGATCAAGGGAGATGGGCTCATGCATATATAGAGAGAAACAAGGTGAGGATGACAGTGACATTGGGGACTGCACTGCTTGACATGTATGCAAAATGTGGGAACATGAGTAAGGCCATGGAAGTGTTTTGGGGGATGAAGGAAAAGAATGTGTATACGTGGAGTGTTGCCTTGGGGGGACTAGCTATGAATGGCTGTGGTGAGAAGTGTCTTGAGCTTTTCTCAGTCATGGAGAAAGAAGGCCTTCGGCCAAATGAAGTTACCTTTGTTTCGGTTCTTAGGGGTTGTGCTGTGGTTGGACTAGTGGAGGAAGGTCGTCGACATTTTGACTTGATGAAAGAATTGTATGGGATTGAACCTCAGCTAGAGCATTATGGGTGCATTGTTGACTTATATGGTCGAGCTGGGCATTTAGATGAAGCCCTGAACTTCATTAACAGCATGCCAATGAAGCCTCATGCGGGTGCGTGGGGGGCTTTGCTCCACGCTAGTAGAATGTATAGGAATATGGAACTGGGTGAGCTTGCGTCGAGAAAGATAATAGAACTCGAGGATAGGAATCACGGTGCTTATGTGCTTTTGTCCAATATATATGCGGATTCCAAGCTTTATGATGGAGTCAGTAATGTGCGGCAAAATATGAAGGCTAAAGGTGTGAGGAAGCTTCCTGGTTGTAGTGTCATAGAAGTTGATGGGGAAGTTCATGAATTCTTTGCAGGGGAGGACAAGTCTCATCCGAGGTATAATGAAATCGAAATGATGCTGGAAGAAATCTCCCGGCGCCTGAAATTAGCAGGTTATGTTGCCAATACTAACCCTGTGCTGTTCGATATAGAAGAAGAAGAGAAAGAGGATGCATTGTTTAAACACAGCGAGAAGTTTGCCATTGCTTTTGGTTTGATCAGTTTGAGAGGAGTACCCATTAGGATTGTGAAGAACCTCAGGTCAAAATTCATGGAAACCGAACCAACCGGTTTGGTCCGAGTTTGGTAA

Protein Analysis

435

Amino Acids

48.65

Weight (kDa)

5.79

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 53 - 101 8.1e-08 PPR repeat family
PPR PF01535 55 - 85 7.4e-07 PPR repeat
PPR_2 PF13041 153 - 201 2.2e-09 PPR repeat family
E_motif PF20431 271 - 333 1.4e-19 E motif
Eplus_motif PF20430 338 - 366 2.7e-06 E+ motif
DYW_deaminase PF14432 370 - 419 5.1e-12 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 1097
AccB1I GGYRCC 1 cut(s) 1092
AccB7I CCANNNNNTGG 1 cut(s) 168
AccI GTMKAC 2 cut(s) 467, 631
AciI CCGC 6 cut(s) 8, 48, 185, 773, 908, 943
AclWI GGATC 2 cut(s) 53, 324
AcoI YGGCCR 1 cut(s) 560
AcsI RAATTY 2 cut(s) 1019, 1262
AcyI GRCGYC 1 cut(s) 1093
AfaI GTAC 1 cut(s) 1230
AfeI AGCGCT 1 cut(s) 94
AfiI CCNNNNNNNGG 2 cut(s) 168, 773
AflIII ACRYGT 1 cut(s) 396
AgeI ACCGGT 1 cut(s) 1283
AgsI TTSAA 1 cut(s) 668
AhlI ACTAGT 1 cut(s) 613
AjnI CCWGG 1 cut(s) 979
AluBI AGCT 8 cut(s) 218, 497, 529, 676, 716, 835, 919, 976
AluI AGCT 8 cut(s) 218, 497, 529, 676, 716, 835, 919, 976
Alw26I GTCTC 2 cut(s) 150, 1047
AlwI GGATC 2 cut(s) 53, 324
Ama87I CYCGRG 1 cut(s) 860
Aor51HI AGCGCT 1 cut(s) 94
AoxI GGCC 3 cut(s) 426, 553, 560
ApoI RAATTY 2 cut(s) 1019, 1262
AsiGI ACCGGT 1 cut(s) 1283
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 3 cut(s) 95, 196, 1095
AspS9I GGNCC 1 cut(s) 1291
AsuC2I CCSGG 1 cut(s) 1090
AsuHPI GGTGA 4 cut(s) 271, 370, 524, 842
AvaI CYCGRG 1 cut(s) 860
AvaII GGWCC 1 cut(s) 1291
AxyI CCTNAGG 1 cut(s) 1253
BaeGI GKGCMC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 1092
BanII GRGCYC 1 cut(s) 334
BbvCI CCTCAGC 1 cut(s) 672
BccI CCATC 3 cut(s) 321, 920, 998
BcgI CGANNNNNNTGC 2 cut(s) 1175, 1209
BciT130I CCWGG 1 cut(s) 981
BclI TGATCA 1 cut(s) 1212
BcnI CCSGG 1 cut(s) 1090
BcoDI GTCTC 2 cut(s) 150, 1047
BcuI ACTAGT 1 cut(s) 613
BfaI CTAG 5 cut(s) 210, 494, 614, 677, 801
BfoI RGCGCY 2 cut(s) 96, 1096
BfuAI ACCTGC 1 cut(s) 1097
BisI GCNGC 2 cut(s) 49, 944
BlsI GCNGC 2 cut(s) 50, 945
Bme1390I CCNGG 2 cut(s) 981, 1090
Bme18I GGWCC 1 cut(s) 1291
BmeT110I CYCGRG 1 cut(s) 860
BmgT120I GGNCC 1 cut(s) 1291
BmiI GGNNCC 1 cut(s) 1094
BmrFI CCNGG 2 cut(s) 981, 1090
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 3 cut(s) 228, 1062, 1156
BmuI ACTGGG 1 cut(s) 836
BplI GAGNNNNNCTC 4 cut(s) 316, 348, 518, 550
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 1090
BsaAI YACGTR 1 cut(s) 471
BsaBI GATNNNNATC 1 cut(s) 870
BsaHI GRCGYC 1 cut(s) 1093
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 429, 483, 1049
BsaWI WCCGGW 1 cut(s) 1283
BsaXI ACNNNNNCTCC 2 cut(s) 1025, 1055
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 773
Bse118I RCCGGY 1 cut(s) 1283
Bse1I ACTGG 2 cut(s) 318, 831
Bse21I CCTNAGG 1 cut(s) 1253
Bse3DI GCAATG 4 cut(s) 35, 51, 203, 1197
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 1 cut(s) 981
BseDI CCNNGG 3 cut(s) 429, 483, 1049
BseGI GGATG 5 cut(s) 118, 369, 453, 1045, 1171
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 2 cut(s) 168, 773
BseMI GCAATG 4 cut(s) 35, 51, 203, 1197
BseMII CTCAG 4 cut(s) 141, 549, 686, 1267
BseNI ACTGG 2 cut(s) 318, 831
BseRI GAGGAG 1 cut(s) 1239
BseSI GKGCMC 1 cut(s) 313
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 3 cut(s) 428, 555, 562
BshNI GGYRCC 1 cut(s) 1092
BshTI ACCGGT 1 cut(s) 1283
BsiHKCI CYCGRG 1 cut(s) 860
BsiSI CCGG 2 cut(s) 1090, 1284
BslFI GGGAC 2 cut(s) 394, 504
BslI CCNNNNNNNGG 2 cut(s) 168, 773
BsmAI GTCTC 2 cut(s) 150, 1047
BsmFI GGGAC 2 cut(s) 394, 504
BsmI GAATGC 2 cut(s) 178, 196
BsnI GGCC 3 cut(s) 428, 555, 562
Bso31I GGTCTC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 860
Bsp1286I GDGCHC 2 cut(s) 313, 334
Bsp143I GATC 3 cut(s) 58, 316, 1212
Bsp19I CCATGG 1 cut(s) 429
BspACI CCGC 6 cut(s) 8, 48, 185, 773, 908, 943
BspANI GGCC 3 cut(s) 428, 555, 562
BspCNI CTCAG 4 cut(s) 142, 548, 685, 1266
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 1 cut(s) 1094
BspMI ACCTGC 1 cut(s) 1097
BspPI GGATC 2 cut(s) 53, 324
BspT107I GGYRCC 1 cut(s) 1092
BspTNI GGTCTC 1 cut(s) 150
BsrDI GCAATG 4 cut(s) 35, 51, 203, 1197
BsrFI RCCGGY 1 cut(s) 1283
BsrI ACTGG 2 cut(s) 318, 831
BssAI RCCGGY 1 cut(s) 1283
BssECI CCNNGG 3 cut(s) 429, 483, 1049
BssMI GATC 3 cut(s) 58, 316, 1212
BssNAI GTATAC 1 cut(s) 468
BssNI GRCGYC 1 cut(s) 1093
BssT1I CCWWGG 2 cut(s) 429, 483
Bst1107I GTATAC 1 cut(s) 468
Bst2UI CCWGG 1 cut(s) 981
Bst4CI ACNGT 3 cut(s) 129, 370, 878
Bst6I CTCTTC 1 cut(s) 1149
BstACI GRCGYC 1 cut(s) 1093
BstBAI YACGTR 1 cut(s) 471
BstC8I GCNNGC 2 cut(s) 755, 837
BstDEI CTNAG 5 cut(s) 150, 535, 590, 672, 1253
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 5 cut(s) 118, 369, 453, 1045, 1171
BstH2I RGCGCY 2 cut(s) 96, 1096
BstHHI GCGC 3 cut(s) 95, 196, 1095
BstKTI GATC 3 cut(s) 61, 319, 1215
BstMAI GTCTC 2 cut(s) 150, 1047
BstMBI GATC 3 cut(s) 58, 316, 1212
BstMWI GCNNNNNNNGC 4 cut(s) 182, 191, 763, 785
BstNI CCWGG 1 cut(s) 981
BstNSI RCATGY 2 cut(s) 400, 757
BstSCI CCNGG 2 cut(s) 979, 1088
BstSLI GKGCMC 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 468
Bsu36I CCTNAGG 1 cut(s) 1253
BsuRI GGCC 3 cut(s) 428, 555, 562
BtgI CCRYGG 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 191
BtsCI GGATG 5 cut(s) 118, 369, 453, 1045, 1171
BtsI GCAGTG 1 cut(s) 386
BtsIMutI CAGTG 3 cut(s) 311, 375, 386
BveI ACCTGC 1 cut(s) 1097
Cac8I GCNNGC 2 cut(s) 755, 837
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 3 cut(s) 95, 196, 1095
Cfr10I RCCGGY 1 cut(s) 1283
Cfr13I GGNCC 1 cut(s) 1291
CseI GACGC 1 cut(s) 828
Csp6I GTAC 1 cut(s) 1229
CspAI ACCGGT 1 cut(s) 1283
CviAII CATG 9 cut(s) 335, 397, 418, 430, 541, 754, 770, 1016, 1267
CviQI GTAC 1 cut(s) 1229
DdeI CTNAG 5 cut(s) 150, 535, 590, 672, 1253
DinI GGCGCC 1 cut(s) 1094
DpnI GATC 3 cut(s) 60, 318, 1214
DpnII GATC 3 cut(s) 58, 316, 1212
DraI TTTAAA 1 cut(s) 1177
EaeI YGGCCR 1 cut(s) 560
Eam1104I CTCTTC 1 cut(s) 1149
EarI CTCTTC 1 cut(s) 1149
Eco130I CCWWGG 2 cut(s) 429, 483
Eco147I AGGCCT 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 1291
Eco47III AGCGCT 1 cut(s) 94
Eco81I CCTNAGG 1 cut(s) 1253
Eco88I CYCGRG 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 979
EcoT14I CCWWGG 2 cut(s) 429, 483
EcoT22I ATGCAT 2 cut(s) 340, 1171
EcoT38I GRGCYC 1 cut(s) 334
EgeI GGCGCC 1 cut(s) 1094
EheI GGCGCC 1 cut(s) 1094
ErhI CCWWGG 2 cut(s) 429, 483
FaeI CATG 9 cut(s) 338, 400, 421, 433, 544, 757, 773, 1019, 1270
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 2 cut(s) 394, 504
FatI CATG 9 cut(s) 334, 396, 417, 429, 540, 753, 769, 1015, 1266
FauI CCCGC 2 cut(s) 178, 766
FbaI TGATCA 1 cut(s) 1212
FblI GTMKAC 2 cut(s) 467, 631
Fnu4HI GCNGC 2 cut(s) 49, 944
FokI GGATG 5 cut(s) 125, 376, 460, 1032, 1178
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 2 cut(s) 49, 944
FspBI CTAG 5 cut(s) 210, 494, 614, 677, 801
FspI TGCGCA 1 cut(s) 195
GlaI GCGC 3 cut(s) 94, 195, 1094
GluI GCNGC 2 cut(s) 49, 944
GsaI CCCAGC 1 cut(s) 720
HaeII RGCGCY 2 cut(s) 96, 1096
HaeIII GGCC 3 cut(s) 428, 555, 562
HapII CCGG 2 cut(s) 1090, 1284
HgaI GACGC 1 cut(s) 828
HhaI GCGC 3 cut(s) 95, 196, 1095
Hin1I GRCGYC 1 cut(s) 1093
Hin1II CATG 9 cut(s) 338, 400, 421, 433, 544, 757, 773, 1019, 1270
Hin6I GCGC 3 cut(s) 93, 194, 1093
HinP1I GCGC 3 cut(s) 93, 194, 1093
HincII GTYRAC 2 cut(s) 632, 700
HindII GTYRAC 2 cut(s) 632, 700
HindIII AAGCTT 3 cut(s) 216, 917, 974
HinfI GANTC 3 cut(s) 871, 911, 930
HpaII CCGG 2 cut(s) 1090, 1284
HphI GGTGA 4 cut(s) 271, 370, 524, 842
Hpy166II GTNNAC 4 cut(s) 262, 468, 632, 700
Hpy188I TCNGA 3 cut(s) 78, 1050, 1295
Hpy188III TCNNGA 4 cut(s) 62, 524, 843, 1016
Hpy8I GTNNAC 4 cut(s) 262, 468, 632, 700
Hpy99I CGWCG 2 cut(s) 633, 844
HpyAV CCTTC 5 cut(s) 445, 545, 566, 617, 949
HpyCH4III ACNGT 3 cut(s) 129, 370, 878
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 8 cut(s) 125, 241, 338, 386, 404, 693, 1028, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 191, 763, 785
HpyF3I CTNAG 5 cut(s) 150, 535, 590, 672, 1253
HpySE526I ACGT 1 cut(s) 470
Hsp92I GRCGYC 1 cut(s) 1093
Hsp92II CATG 9 cut(s) 338, 400, 421, 433, 544, 757, 773, 1019, 1270
HspAI GCGC 3 cut(s) 93, 194, 1093
KasI GGCGCC 1 cut(s) 1092
Ksp22I TGATCA 1 cut(s) 1212
Kzo9I GATC 3 cut(s) 58, 316, 1212
LmnI GCTCC 2 cut(s) 25, 798
LweI GCATC 3 cut(s) 228, 1062, 1156
MaeI CTAG 5 cut(s) 210, 494, 614, 677, 801
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 3 cut(s) 299, 370, 571
MalI GATC 3 cut(s) 60, 318, 1214
MboI GATC 3 cut(s) 58, 316, 1212
MboII GAAGA 5 cut(s) 1091, 1160, 1163, 1166, 1258
MhlI GDGCHC 2 cut(s) 313, 334
MluCI AATT 5 cut(s) 11, 653, 1019, 1100, 1262
Mly113I GGCGCC 1 cut(s) 1093
MlyI GAGTC 1 cut(s) 939
MmeI TCCRAC 1 cut(s) 589
Mph1103I ATGCAT 2 cut(s) 340, 1171
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 2 cut(s) 747, 1176
MslI CAYNNNNRTG 4 cut(s) 434, 726, 758, 774
MspI CCGG 2 cut(s) 1090, 1284
MspR9I CCNGG 2 cut(s) 981, 1090
MssI GTTTAAAC 1 cut(s) 1177
Mva1269I GAATGC 2 cut(s) 178, 196
MvaI CCWGG 1 cut(s) 981
MwoI GCNNNNNNNGC 4 cut(s) 182, 191, 763, 785
NarI GGCGCC 1 cut(s) 1093
NciI CCSGG 1 cut(s) 1090
NcoI CCATGG 1 cut(s) 429
NdeII GATC 3 cut(s) 58, 316, 1212
NlaIII CATG 9 cut(s) 338, 400, 421, 433, 544, 757, 773, 1019, 1270
NlaIV GGNNCC 1 cut(s) 1094
NmuCI GTSAC 2 cut(s) 299, 370
NsbI TGCGCA 1 cut(s) 195
NsiI ATGCAT 2 cut(s) 340, 1171
NspI RCATGY 2 cut(s) 400, 757
PaeI GCATGC 1 cut(s) 757
PaeR7I CTCGAG 1 cut(s) 860
PagI TCATGA 1 cut(s) 1015
PceI AGGCCT 1 cut(s) 555
PciI ACATGT 1 cut(s) 396
PctI GAATGC 2 cut(s) 178, 196
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 871, 911
PflMI CCANNNNNTGG 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 1283
PkrI GCNGC 2 cut(s) 50, 945
PleI GAGTC 1 cut(s) 938
PluTI GGCGCC 1 cut(s) 1096
PmeI GTTTAAAC 1 cut(s) 1177
PpsI GAGTC 1 cut(s) 938
Ppu21I YACGTR 1 cut(s) 471
PscI ACATGT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 979
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 1 cut(s) 979
PspN4I GGNNCC 1 cut(s) 1094
PspPI GGNCC 1 cut(s) 1291
PspXI VCTCGAGB 1 cut(s) 860
RsaI GTAC 1 cut(s) 1230
RsaNI GTAC 1 cut(s) 1229
RseI CAYNNNNRTG 4 cut(s) 434, 726, 758, 774
SalI GTCGAC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 747, 1176
SatI GCNGC 2 cut(s) 49, 944
Sau3AI GATC 3 cut(s) 58, 316, 1212
Sau96I GGNCC 1 cut(s) 1291
SchI GAGTC 1 cut(s) 939
ScrFI CCNGG 2 cut(s) 981, 1090
SduI GDGCHC 2 cut(s) 313, 334
SfaNI GCATC 3 cut(s) 228, 1062, 1156
SfoI GGCGCC 1 cut(s) 1094
Sfr274I CTCGAG 1 cut(s) 860
SinI GGWCC 1 cut(s) 1291
SlaI CTCGAG 1 cut(s) 860
SmiMI CAYNNNNRTG 4 cut(s) 434, 726, 758, 774
SmlI CTYRAG 2 cut(s) 524, 860
SmoI CTYRAG 2 cut(s) 524, 860
SpeI ACTAGT 1 cut(s) 613
SphI GCATGC 1 cut(s) 757
Sse9I AATT 5 cut(s) 11, 653, 1019, 1100, 1262
SseBI AGGCCT 1 cut(s) 555
SsiI CCGC 6 cut(s) 8, 48, 185, 773, 908, 943
SspDI GGCGCC 1 cut(s) 1092
SspMI CTAG 5 cut(s) 210, 494, 614, 677, 801
StuI AGGCCT 1 cut(s) 555
StyD4I CCNGG 2 cut(s) 979, 1088
StyI CCWWGG 2 cut(s) 429, 483
TaaI ACNGT 3 cut(s) 129, 370, 878
TaiI ACGT 1 cut(s) 473
TaqI TCGA 7 cut(s) 138, 631, 712, 842, 861, 1065, 1140
TasI AATT 5 cut(s) 11, 653, 1019, 1100, 1262
TauI GCSGC 2 cut(s) 51, 946
TfiI GAWTC 2 cut(s) 871, 911
Tru1I TTAA 2 cut(s) 747, 1176
Tru9I TTAA 2 cut(s) 747, 1176
TscAI CASTG 3 cut(s) 318, 375, 393
TseFI GTSAC 2 cut(s) 299, 370
Tsp45I GTSAC 2 cut(s) 299, 370
TspRI CASTG 3 cut(s) 318, 375, 393
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 1291
XapI RAATTY 2 cut(s) 1019, 1262
XceI RCATGY 2 cut(s) 400, 757
XhoI CTCGAG 1 cut(s) 860
XmiI GTMKAC 2 cut(s) 467, 631
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 5 cut(s) 210, 494, 614, 677, 801
Zsp2I ATGCAT 2 cut(s) 340, 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.