Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004653

Physical Location & Seq

Genomic Coordinates Reverse (-)

5750247 .. 5751759

Gene Structure 1513 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1081900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 705 bp

ATGGGCGAGAGCGACGTCAAAGTTCTGGGAATGGCGCCGAGTCCGTTCGTGATGAGGGCTCGCATTGCCCTTAACCTCAAATCCGTCGACTACGAGTTTCTTCAGGAGACATTCGGATCTAAAAGCGAGCTTCTTCTTCAGTCCAATCCAGTCCACAAGAAAGTCCCAGTTCTCATTCATGGTGACAAACCAGTTTGTGAATCTCTCATCATTGTTGAGTACATTGACGAAGTTTGGGCATCTGGTCCGTCTATCCTCCCTTCTGACCCCTACGATCGCGCTACTGCACGATTTTGGGCTGCCTATATCAGCGAGAAGTGGTACCCATCCATGAAAGGCATTGGTCTTGCACAAGGAGAGGAGGCAAAGAAGGCAGCAATCGAGCAAGTGACAGAAGGGCTGGCGCTGCTGGAGGAAGCTTTTGAGAAATCCAGCAAAGGCAACGTATTTTTCGGTGGAGATGAGATCGGATACTTGGACATTGCGTTCGGGTGCTTCTTGGGGTGGCTCAGAGTAAACGAGAAGCTGCATGGAATCAAACTGCTTGACCAGACGAAAACGCCGGGGCTGGTAAAATGGGCTGACAAGTTTTGTGCTCATGCTGCTGTGAAGGATGTTATGCCTGAAACTGACAAGCTTGTTGAGATTGCTAAGATTCTTGCTGCCAAAGCAAGGGCTGCAGCCGCTCCTCCTCCGTCTAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009314 GO:0009404 GO:0009407 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009636 GO:0009639 GO:0009640 GO:0009651 GO:0009704 GO:0009719 GO:0009725 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010243 GO:0016740 GO:0016765 GO:0019748 GO:0022622 GO:0032501 GO:0032502 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043434 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048364 GO:0048519 GO:0048527 GO:0048528 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051186 GO:0060416 GO:0065007 GO:0071704 GO:0080134 GO:0080148 GO:0080167 GO:0090696 GO:0098754 GO:0099402 GO:1901564 GO:1901652 GO:1901698 GO:1901700 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_C_2 GST_C_3 GST_N GST_N_3

Protein Analysis

235

Amino Acids

25.62

Weight (kDa)

5.67

Isoelectric Point (pI)

38.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	5 - 77	7e-19	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	10 - 77	1.2e-14	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	14 - 77	8.9e-12	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	101 - 200	5.3e-08	Glutathione S-transferase, C-terminal domain
GST_C_7	PF22041	120 - 172	2.2e-06	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	121 - 194	9.2e-09	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10360 AT1G10370
fragaria_vesca	FvH4_4g35560 FvH4_4g35600
malus_domestica	MD13G1081900.v1.1 MD13G1082000.v1.1 MD13G1082100.v1.1 MD13G1082200.v1.1 MD16G1081100.v1.1
prunus_persica	Prupe.1G264700_v2.0.a1 Prupe.1G264900_v2.0.a1
pyrus_communis	pycom13g07080 pycom16g07010
rosa_chinensis	RchiOBHm_Chr4g0445201 RchiOBHm_Chr5g0060741
rosa_laevigata	RLG00000005757 RLG00000035391
rosa_multiflora	Rmu_co8192854.1_g000001 Rmu_sc0000050.1_g000034 Rmu_sc0006568.1_g000003 Rmu_sc0029476.1_g000002
rosa_roxburghii	Rroxscaffold_5G00385720
rosa_rugosa	Rorug04G0360000 Rorug05G0333500
rosa_samantha	Rh4AG420800 Rh4BG432700 Rh4CG447400 Rh4DG428800 Rh5BG408500 Rh5DG423700
rosa_wichuraiana	Rw4G036090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	18
Acc65I	GGTACC	1 cut(s)	321
AccB1I	GGYRCC	2 cut(s)	34, 321
AccBSI	CCGCTC	1 cut(s)	686
AccI	GTMKAC	1 cut(s)	87
AccII	CGCG	1 cut(s)	279
AciI	CCGC	1 cut(s)	684
AclWI	GGATC	1 cut(s)	124
AcuI	CTGAAG	2 cut(s)	86, 122
AcyI	GRCGYC	2 cut(s)	15, 35
AfaI	GTAC	2 cut(s)	221, 323
AfiI	CCNNNNNNNGG	1 cut(s)	672
AloI	GAACNNNNNNTCC	2 cut(s)	470, 502
AluBI	AGCT	4 cut(s)	130, 419, 526, 637
AluI	AGCT	4 cut(s)	130, 419, 526, 637
Alw21I	GWGCWC	1 cut(s)	598
Alw26I	GTCTC	1 cut(s)	101
AlwI	GGATC	1 cut(s)	124
ApeKI	GCWGC	8 cut(s)	299, 374, 406, 526, 602, 662, 677, 680
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
Asp718I	GGTACC	1 cut(s)	321
AspLEI	GCGC	3 cut(s)	37, 281, 406
AspS9I	GGNCC	1 cut(s)	245
AsuC2I	CCSGG	1 cut(s)	564
AsuHPI	GGTGA	1 cut(s)	194
AvaII	GGWCC	1 cut(s)	245
BanI	GGYRCC	2 cut(s)	34, 321
BanII	GRGCYC	1 cut(s)	61
Bbv12I	GWGCWC	1 cut(s)	598
BbvI	GCAGC	8 cut(s)	286, 386, 393, 513, 589, 649, 664, 692
BccI	CCATC	1 cut(s)	334
BciVI	GTATCC	1 cut(s)	464
BcnI	CCSGG	1 cut(s)	564
BcoDI	GTCTC	1 cut(s)	101
BfmI	CTRYAG	1 cut(s)	678
BfoI	RGCGCY	2 cut(s)	38, 407
BfuI	GTATCC	1 cut(s)	464
BisI	GCNGC	9 cut(s)	300, 375, 407, 527, 603, 663, 678, 681, 684
BlsI	GCNGC	9 cut(s)	301, 376, 408, 528, 604, 664, 679, 682, 685
Bme1390I	CCNGG	1 cut(s)	564
Bme18I	GGWCC	1 cut(s)	245
BmgT120I	GGNCC	1 cut(s)	245
BmiI	GGNNCC	2 cut(s)	36, 323
BmrFI	CCNGG	1 cut(s)	564
BmrI	ACTGGG	1 cut(s)	161
BmsI	GCATC	1 cut(s)	248
BmuI	ACTGGG	1 cut(s)	161
BpmI	CTGGAG	1 cut(s)	431
BpuMI	CCSGG	1 cut(s)	564
BsaHI	GRCGYC	2 cut(s)	15, 35
BsaJI	CCNNGG	1 cut(s)	563
Bsc4I	CCNNNNNNNGG	1 cut(s)	672
Bse1I	ACTGG	3 cut(s)	149, 167, 191
Bse3DI	GCAATG	2 cut(s)	63, 480
BseDI	CCNNGG	1 cut(s)	563
BseGI	GGATG	2 cut(s)	326, 619
BseLI	CCNNNNNNNGG	1 cut(s)	672
BseMI	GCAATG	2 cut(s)	63, 480
BseMII	CTCAG	1 cut(s)	523
BseNI	ACTGG	3 cut(s)	149, 167, 191
BseRI	GAGGAG	3 cut(s)	374, 678, 681
BseXI	GCAGC	8 cut(s)	286, 386, 393, 513, 589, 649, 664, 692
BsgI	GTGCAG	1 cut(s)	270
Bsh1236I	CGCG	1 cut(s)	279
Bsh1285I	CGRYCG	1 cut(s)	277
BshNI	GGYRCC	2 cut(s)	34, 321
BsiEI	CGRYCG	1 cut(s)	277
BsiHKAI	GWGCWC	1 cut(s)	598
BsiSI	CCGG	1 cut(s)	563
BslFI	GGGAC	1 cut(s)	149
BslI	CCNNNNNNNGG	1 cut(s)	672
BsmAI	GTCTC	1 cut(s)	101
BsmFI	GGGAC	1 cut(s)	149
Bsp1286I	GDGCHC	2 cut(s)	61, 598
Bsp143I	GATC	3 cut(s)	116, 274, 465
BspACI	CCGC	1 cut(s)	684
BspCNI	CTCAG	1 cut(s)	522
BspFNI	CGCG	1 cut(s)	279
BspLI	GGNNCC	2 cut(s)	36, 323
BspMAI	CTGCAG	1 cut(s)	682
BspPI	GGATC	1 cut(s)	124
BspT107I	GGYRCC	2 cut(s)	34, 321
BsrBI	CCGCTC	1 cut(s)	686
BsrDI	GCAATG	2 cut(s)	63, 480
BsrI	ACTGG	3 cut(s)	149, 167, 191
BssECI	CCNNGG	1 cut(s)	563
BssMI	GATC	3 cut(s)	116, 274, 465
BssNI	GRCGYC	2 cut(s)	15, 35
BstACI	GRCGYC	2 cut(s)	15, 35
BstAPI	GCANNNNNTGC	1 cut(s)	677
BstC8I	GCNNGC	3 cut(s)	61, 128, 402
BstDEI	CTNAG	2 cut(s)	509, 651
BstF5I	GGATG	2 cut(s)	326, 619
BstFNI	CGCG	1 cut(s)	279
BstH2I	RGCGCY	2 cut(s)	38, 407
BstHHI	GCGC	3 cut(s)	37, 281, 406
BstKTI	GATC	3 cut(s)	119, 277, 468
BstMAI	GTCTC	1 cut(s)	101
BstMBI	GATC	3 cut(s)	116, 274, 465
BstMCI	CGRYCG	1 cut(s)	277
BstMWI	GCNNNNNNNGC	7 cut(s)	65, 371, 406, 602, 668, 677, 683
BstSCI	CCNGG	1 cut(s)	562
BstSFI	CTRYAG	1 cut(s)	678
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	8 cut(s)	286, 386, 393, 513, 589, 649, 664, 692
BstX2I	RGATCY	1 cut(s)	116
BstYI	RGATCY	1 cut(s)	116
BsuI	GTATCC	1 cut(s)	464
BtsCI	GGATG	2 cut(s)	326, 619
Cac8I	GCNNGC	3 cut(s)	61, 128, 402
CfoI	GCGC	3 cut(s)	37, 281, 406
Cfr13I	GGNCC	1 cut(s)	245
Csp6I	GTAC	2 cut(s)	220, 322
CviAII	CATG	4 cut(s)	179, 331, 530, 599
CviQI	GTAC	2 cut(s)	220, 322
DdeI	CTNAG	2 cut(s)	509, 651
DinI	GGCGCC	1 cut(s)	36
DpnI	GATC	3 cut(s)	118, 276, 467
DpnII	GATC	3 cut(s)	116, 274, 465
Eco24I	GRGCYC	1 cut(s)	61
Eco47I	GGWCC	1 cut(s)	245
Eco57I	CTGAAG	2 cut(s)	86, 122
EcoT38I	GRGCYC	1 cut(s)	61
EgeI	GGCGCC	1 cut(s)	36
EheI	GGCGCC	1 cut(s)	36
FaeI	CATG	4 cut(s)	182, 334, 533, 602
FaiI	YATR	6 cut(s)	180, 306, 332, 531, 600, 620
FaqI	GGGAC	1 cut(s)	149
FatI	CATG	4 cut(s)	178, 330, 529, 598
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	9 cut(s)	300, 375, 407, 527, 603, 663, 678, 681, 684
FokI	GGATG	2 cut(s)	313, 626
FriOI	GRGCYC	1 cut(s)	61
Fsp4HI	GCNGC	9 cut(s)	300, 375, 407, 527, 603, 663, 678, 681, 684
GlaI	GCGC	3 cut(s)	36, 280, 405
GluI	GCNGC	9 cut(s)	300, 375, 407, 527, 603, 663, 678, 681, 684
GsuI	CTGGAG	1 cut(s)	431
HaeII	RGCGCY	2 cut(s)	38, 407
HapII	CCGG	1 cut(s)	563
HhaI	GCGC	3 cut(s)	37, 281, 406
Hin1I	GRCGYC	2 cut(s)	15, 35
Hin1II	CATG	4 cut(s)	182, 334, 533, 602
Hin6I	GCGC	3 cut(s)	35, 279, 404
HinP1I	GCGC	3 cut(s)	35, 279, 404
HincII	GTYRAC	1 cut(s)	88
HindII	GTYRAC	1 cut(s)	88
HindIII	AAGCTT	2 cut(s)	417, 635
HinfI	GANTC	4 cut(s)	40, 200, 534, 655
HpaII	CCGG	1 cut(s)	563
HphI	GGTGA	1 cut(s)	194
Hpy166II	GTNNAC	3 cut(s)	88, 154, 517
Hpy188I	TCNGA	4 cut(s)	116, 265, 470, 512
Hpy188III	TCNNGA	2 cut(s)	49, 104
Hpy8I	GTNNAC	3 cut(s)	88, 154, 517
Hpy99I	CGWCG	2 cut(s)	17, 89
HpyAV	CCTTC	4 cut(s)	270, 364, 389, 604
HpyCH4IV	ACGT	2 cut(s)	15, 444
HpyCH4V	TGCA	4 cut(s)	287, 350, 529, 680
HpyF10VI	GCNNNNNNNGC	7 cut(s)	65, 371, 406, 602, 668, 677, 683
HpyF3I	CTNAG	2 cut(s)	509, 651
HpySE526I	ACGT	2 cut(s)	15, 444
Hsp92I	GRCGYC	2 cut(s)	15, 35
Hsp92II	CATG	4 cut(s)	182, 334, 533, 602
HspAI	GCGC	3 cut(s)	35, 279, 404
KasI	GGCGCC	1 cut(s)	34
KpnI	GGTACC	1 cut(s)	325
Kzo9I	GATC	3 cut(s)	116, 274, 465
LmnI	GCTCC	1 cut(s)	691
Lsp1109I	GCAGC	8 cut(s)	286, 386, 393, 513, 589, 649, 664, 692
LweI	GCATC	1 cut(s)	248
MaeII	ACGT	2 cut(s)	15, 444
MaeIII	GTNAC	2 cut(s)	182, 388
MalI	GATC	3 cut(s)	118, 276, 467
MbiI	CCGCTC	1 cut(s)	686
MboI	GATC	3 cut(s)	116, 274, 465
MboII	GAAGA	3 cut(s)	92, 125, 128
MflI	RGATCY	1 cut(s)	116
MhlI	GDGCHC	2 cut(s)	61, 598
MluCI	AATT	1 cut(s)	700
Mly113I	GGCGCC	1 cut(s)	35
MlyI	GAGTC	1 cut(s)	49
MnlI	CCTC	8 cut(s)	48, 86, 266, 352, 355, 406, 699, 702
MseI	TTAA	1 cut(s)	72
MspI	CCGG	1 cut(s)	563
MspR9I	CCNGG	1 cut(s)	564
MvnI	CGCG	1 cut(s)	279
MwoI	GCNNNNNNNGC	7 cut(s)	65, 371, 406, 602, 668, 677, 683
NarI	GGCGCC	1 cut(s)	35
NciI	CCSGG	1 cut(s)	564
NdeII	GATC	3 cut(s)	116, 274, 465
NlaIII	CATG	4 cut(s)	182, 334, 533, 602
NlaIV	GGNNCC	2 cut(s)	36, 323
NmeAIII	GCCGAG	1 cut(s)	63
NmuCI	GTSAC	2 cut(s)	182, 388
PfeI	GAWTC	3 cut(s)	200, 534, 655
PkrI	GCNGC	9 cut(s)	301, 376, 408, 528, 604, 664, 679, 682, 685
Ple19I	CGATCG	1 cut(s)	277
PleI	GAGTC	1 cut(s)	48
PluTI	GGCGCC	1 cut(s)	38
PpsI	GAGTC	1 cut(s)	48
PspN4I	GGNNCC	2 cut(s)	36, 323
PspPI	GGNCC	1 cut(s)	245
PstI	CTGCAG	1 cut(s)	682
PsuI	RGATCY	1 cut(s)	116
PvuI	CGATCG	1 cut(s)	277
RsaI	GTAC	2 cut(s)	221, 323
RsaNI	GTAC	2 cut(s)	220, 322
SalI	GTCGAC	1 cut(s)	86
SaqAI	TTAA	1 cut(s)	72
SatI	GCNGC	9 cut(s)	300, 375, 407, 527, 603, 663, 678, 681, 684
Sau3AI	GATC	3 cut(s)	116, 274, 465
Sau96I	GGNCC	1 cut(s)	245
SchI	GAGTC	1 cut(s)	49
ScrFI	CCNGG	1 cut(s)	564
SduI	GDGCHC	2 cut(s)	61, 598
SetI	ASST	7 cut(s)	18, 78, 132, 421, 447, 528, 639
SfaNI	GCATC	1 cut(s)	248
SfcI	CTRYAG	1 cut(s)	678
SfoI	GGCGCC	1 cut(s)	36
SinI	GGWCC	1 cut(s)	245
Sse9I	AATT	1 cut(s)	700
SsiI	CCGC	1 cut(s)	684
SspDI	GGCGCC	1 cut(s)	34
StyD4I	CCNGG	1 cut(s)	562
TaiI	ACGT	2 cut(s)	18, 447
TaqI	TCGA	2 cut(s)	87, 381
TasI	AATT	1 cut(s)	700
TatI	WGTACW	1 cut(s)	219
TauI	GCSGC	1 cut(s)	686
TfiI	GAWTC	3 cut(s)	200, 534, 655
Tru1I	TTAA	1 cut(s)	72
Tru9I	TTAA	1 cut(s)	72
TseFI	GTSAC	2 cut(s)	182, 388
TseI	GCWGC	8 cut(s)	299, 374, 406, 526, 602, 662, 677, 680
Tsp45I	GTSAC	2 cut(s)	182, 388
TspDTI	ATGAA	2 cut(s)	167, 347
TspGWI	ACGGA	4 cut(s)	33, 73, 237, 684
VpaK11BI	GGWCC	1 cut(s)	245
XmiI	GTMKAC	1 cut(s)	87
ZraI	GACGTC	1 cut(s)	16

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.