Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004653

Physical Location & Seq

Genomic Coordinates Reverse (-)

5752474 .. 5755270

Gene Structure 2797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1082000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 696 bp

ATGGCGAAGAGCGACGTGAAGGTTTTGGGAGCGTGGCCGAGCCCATACGTGATGAGGGCTCGGATTGCGCTCAATGTCAAGTCAGTGGAGTATGAGCTTCTTGAGGAGACGTTCGCACCCAAAAGCCAGCTTCTTCTCCAGTCCAATCCAGTCCACAAGAAAATCCCAGTTCTCATTCATGGTGATAAGCCCGTCTGTGAGTCTCTCATAATTGTGGAGTACATCGATGAGGTTTGGGCCTCAGGCCCGTCGATTCTCCCTTCTGACCCGTTGGATCGCGCTACTGCAAGATTTTGGGCTGCCTACGTCGATGAGAAGTGGTTTCCGTCGCTGAGAGGCATTGGTGCCGCTGAAGGAGACGAGGCAAGGAAGGCAGCAGTCGAGCAAGTTGCAGAAGGGCTGGCTCAAATGGAGGAAGCCTTTCAAAAGACCAGCAAAGGCAAGGACTTCTTCAGTGGGGACAAAATTGGGTACCTGGACATTGCATTCGGGTGCTTCTTGGGGTGGCTCAGAGTGACAGAGAAAATGAATGGGGTCAAACTTCTGGACGAAGCAAAGATCCCAGGGCTGGCCAAATGGGCTGAGAAGTTCAGTGCAGATCCTGCTGTGAAGGATGTTATGCCGGAGACTGACAAGTTGGCTGAGGTTGCTAAGATTATTATGGCCAGATTGAGAGCTGCAGGTGCTTCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009314 GO:0009404 GO:0009407 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009636 GO:0009639 GO:0009640 GO:0009651 GO:0009704 GO:0009719 GO:0009725 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010243 GO:0016740 GO:0016765 GO:0019748 GO:0022622 GO:0032501 GO:0032502 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043434 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048364 GO:0048519 GO:0048527 GO:0048528 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051186 GO:0060416 GO:0065007 GO:0071704 GO:0080134 GO:0080148 GO:0080167 GO:0090696 GO:0098754 GO:0099402 GO:1901564 GO:1901652 GO:1901698 GO:1901700 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_C_2 GST_C_3 GST_N GST_N_3

Protein Analysis

232

Amino Acids

25.42

Weight (kDa)

5.86

Isoelectric Point (pI)

27.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	5 - 77	4.7e-19	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	14 - 77	8.3e-13	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	14 - 77	8.6e-11	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	114 - 201	3.7e-09	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	120 - 195	1e-09	Glutathione S-transferase, C-terminal domain
GST_C_7	PF22041	120 - 175	2.7e-07	Glutathione S-transferase, C-terminal domain
GST_C_3	PF14497	123 - 205	6.6e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10360 AT1G10370
fragaria_vesca	FvH4_4g35560 FvH4_4g35600
malus_domestica	MD13G1081900.v1.1 MD13G1082000.v1.1 MD13G1082100.v1.1 MD13G1082200.v1.1 MD16G1081100.v1.1
prunus_persica	Prupe.1G264700_v2.0.a1 Prupe.1G264900_v2.0.a1
pyrus_communis	pycom13g07080 pycom16g07010
rosa_chinensis	RchiOBHm_Chr4g0445201 RchiOBHm_Chr5g0060741
rosa_laevigata	RLG00000005757 RLG00000035391
rosa_multiflora	Rmu_co8192854.1_g000001 Rmu_sc0000050.1_g000034 Rmu_sc0006568.1_g000003 Rmu_sc0029476.1_g000002
rosa_roxburghii	Rroxscaffold_5G00385720
rosa_rugosa	Rorug04G0360000 Rorug05G0333500
rosa_samantha	Rh4AG420800 Rh4BG432700 Rh4CG447400 Rh4DG428800 Rh5BG408500 Rh5DG423700
rosa_wichuraiana	Rw4G036090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	671
Acc36I	ACCTGC	1 cut(s)	671
Acc65I	GGTACC	1 cut(s)	471
AccB1I	GGYRCC	2 cut(s)	344, 471
AccII	CGCG	1 cut(s)	279
AciI	CCGC	1 cut(s)	348
AclWI	GGATC	3 cut(s)	282, 553, 593
AcoI	YGGCCR	3 cut(s)	35, 570, 663
AcuI	CTGAAG	2 cut(s)	372, 436
AfaI	GTAC	2 cut(s)	221, 473
AfiI	CCNNNNNNNGG	1 cut(s)	568
AgsI	TTSAA	1 cut(s)	425
AjiI	CACGTC	1 cut(s)	16
AjnI	CCWGG	2 cut(s)	474, 562
AluBI	AGCT	3 cut(s)	97, 130, 677
AluI	AGCT	3 cut(s)	97, 130, 677
Alw26I	GTCTC	4 cut(s)	101, 207, 351, 620
AlwI	GGATC	3 cut(s)	282, 553, 593
AlwNI	CAGNNNCTG	1 cut(s)	602
AoxI	GGCC	5 cut(s)	35, 237, 244, 570, 663
ApeKI	GCWGC	3 cut(s)	299, 374, 677
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
Asp700I	GAANNNNTTC	1 cut(s)	420
Asp718I	GGTACC	1 cut(s)	471
AspLEI	GCGC	2 cut(s)	70, 281
AspS9I	GGNCC	2 cut(s)	237, 245
AsuHPI	GGTGA	1 cut(s)	194
AxyI	CCTNAGG	1 cut(s)	241
BalI	TGGCCA	2 cut(s)	572, 665
BanI	GGYRCC	2 cut(s)	344, 471
BanII	GRGCYC	2 cut(s)	44, 61
BbvCI	CCTCAGC	1 cut(s)	642
BbvI	GCAGC	3 cut(s)	286, 386, 664
BcgI	CGANNNNNNTGC	2 cut(s)	371, 405
BciT130I	CCWGG	2 cut(s)	476, 564
BcoDI	GTCTC	4 cut(s)	101, 207, 351, 620
BfmI	CTRYAG	1 cut(s)	678
BfuAI	ACCTGC	1 cut(s)	671
BglI	GCCNNNNNGGC	1 cut(s)	578
BisI	GCNGC	4 cut(s)	300, 348, 375, 678
BlsI	GCNGC	4 cut(s)	301, 349, 376, 679
Bme1390I	CCNGG	2 cut(s)	476, 564
BmgBI	CACGTC	1 cut(s)	16
BmgT120I	GGNCC	2 cut(s)	237, 245
BmiI	GGNNCC	2 cut(s)	346, 473
BmrFI	CCNGG	2 cut(s)	476, 564
BmrI	ACTGGG	1 cut(s)	161
BmuI	ACTGGG	1 cut(s)	161
BpmI	CTGGAG	1 cut(s)	122
Bpu10I	CCTNAGC	1 cut(s)	642
BpuEI	CTTGAG	1 cut(s)	122
Bsa29I	ATCGAT	1 cut(s)	225
BsaAI	YACGTR	1 cut(s)	49
BsaJI	CCNNGG	2 cut(s)	562, 563
Bsc4I	CCNNNNNNNGG	1 cut(s)	568
Bse1I	ACTGG	3 cut(s)	139, 149, 167
Bse21I	CCTNAGG	1 cut(s)	241
Bse3DI	GCAATG	1 cut(s)	480
BseBI	CCWGG	2 cut(s)	476, 564
BseCI	ATCGAT	1 cut(s)	225
BseDI	CCNNGG	2 cut(s)	562, 563
BseGI	GGATG	1 cut(s)	619
BseLI	CCNNNNNNNGG	1 cut(s)	568
BseMI	GCAATG	1 cut(s)	480
BseMII	CTCAG	5 cut(s)	255, 323, 523, 573, 633
BseNI	ACTGG	3 cut(s)	139, 149, 167
BseRI	GAGGAG	1 cut(s)	119
BseXI	GCAGC	3 cut(s)	286, 386, 664
BsgI	GTGCAG	1 cut(s)	615
Bsh1236I	CGCG	1 cut(s)	279
BshFI	GGCC	5 cut(s)	37, 239, 246, 572, 665
BshNI	GGYRCC	2 cut(s)	344, 471
BshVI	ATCGAT	1 cut(s)	225
BsiSI	CCGG	1 cut(s)	623
BslFI	GGGAC	1 cut(s)	473
BslI	CCNNNNNNNGG	1 cut(s)	568
BsmAI	GTCTC	4 cut(s)	101, 207, 351, 620
BsmBI	CGTCTC	2 cut(s)	101, 351
BsmFI	GGGAC	1 cut(s)	473
BsmI	GAATGC	1 cut(s)	485
BsnI	GGCC	5 cut(s)	37, 239, 246, 572, 665
Bsp1286I	GDGCHC	2 cut(s)	44, 61
Bsp143I	GATC	3 cut(s)	274, 558, 598
BspACI	CCGC	1 cut(s)	348
BspANI	GGCC	5 cut(s)	37, 239, 246, 572, 665
BspCNI	CTCAG	5 cut(s)	254, 324, 522, 574, 634
BspDI	ATCGAT	1 cut(s)	225
BspFNI	CGCG	1 cut(s)	279
BspLI	GGNNCC	2 cut(s)	346, 473
BspMAI	CTGCAG	1 cut(s)	682
BspMI	ACCTGC	1 cut(s)	671
BspPI	GGATC	3 cut(s)	282, 553, 593
BspQI	GCTCTTC	1 cut(s)	2
BspT107I	GGYRCC	2 cut(s)	344, 471
BsrDI	GCAATG	1 cut(s)	480
BsrI	ACTGG	3 cut(s)	139, 149, 167
BssECI	CCNNGG	2 cut(s)	562, 563
BssMI	GATC	3 cut(s)	274, 558, 598
Bst2UI	CCWGG	2 cut(s)	476, 564
Bst6I	CTCTTC	1 cut(s)	2
BstAPI	GCANNNNNTGC	1 cut(s)	602
BstBAI	YACGTR	1 cut(s)	49
BstC8I	GCNNGC	3 cut(s)	128, 402, 570
BstDEI	CTNAG	6 cut(s)	241, 332, 509, 582, 642, 651
BstF5I	GGATG	1 cut(s)	619
BstFNI	CGCG	1 cut(s)	279
BstHHI	GCGC	2 cut(s)	70, 281
BstKTI	GATC	3 cut(s)	277, 561, 601
BstMAI	GTCTC	4 cut(s)	101, 207, 351, 620
BstMBI	GATC	3 cut(s)	274, 558, 598
BstMWI	GCNNNNNNNGC	6 cut(s)	65, 371, 578, 602, 647, 683
BstNI	CCWGG	2 cut(s)	476, 564
BstSCI	CCNGG	2 cut(s)	474, 562
BstSFI	CTRYAG	1 cut(s)	678
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	3 cut(s)	286, 386, 664
BstX2I	RGATCY	2 cut(s)	558, 598
BstYI	RGATCY	2 cut(s)	558, 598
Bsu15I	ATCGAT	1 cut(s)	225
Bsu36I	CCTNAGG	1 cut(s)	241
BsuRI	GGCC	5 cut(s)	37, 239, 246, 572, 665
BsuTUI	ATCGAT	1 cut(s)	225
BtrI	CACGTC	1 cut(s)	16
BtsCI	GGATG	1 cut(s)	619
BtsIMutI	CAGTG	3 cut(s)	90, 460, 598
BveI	ACCTGC	1 cut(s)	671
Cac8I	GCNNGC	3 cut(s)	128, 402, 570
CaiI	CAGNNNCTG	1 cut(s)	602
CfoI	GCGC	2 cut(s)	70, 281
Cfr13I	GGNCC	2 cut(s)	237, 245
ClaI	ATCGAT	1 cut(s)	225
Csp6I	GTAC	2 cut(s)	220, 472
CviAII	CATG	1 cut(s)	179
CviQI	GTAC	2 cut(s)	220, 472
DdeI	CTNAG	6 cut(s)	241, 332, 509, 582, 642, 651
DpnI	GATC	3 cut(s)	276, 560, 600
DpnII	GATC	3 cut(s)	274, 558, 598
EaeI	YGGCCR	3 cut(s)	35, 570, 663
Eam1104I	CTCTTC	1 cut(s)	2
EarI	CTCTTC	1 cut(s)	2
Eco24I	GRGCYC	2 cut(s)	44, 61
Eco57I	CTGAAG	2 cut(s)	372, 436
Eco81I	CCTNAGG	1 cut(s)	241
EcoRII	CCWGG	2 cut(s)	474, 562
EcoT38I	GRGCYC	2 cut(s)	44, 61
Esp3I	CGTCTC	2 cut(s)	101, 351
FaeI	CATG	1 cut(s)	182
FaiI	YATR	6 cut(s)	46, 93, 180, 209, 620, 662
FalI	AAGNNNNNCTT	2 cut(s)	434, 466
FaqI	GGGAC	1 cut(s)	473
FatI	CATG	1 cut(s)	178
Fnu4HI	GCNGC	4 cut(s)	300, 348, 375, 678
FokI	GGATG	1 cut(s)	626
FriOI	GRGCYC	2 cut(s)	44, 61
Fsp4HI	GCNGC	4 cut(s)	300, 348, 375, 678
GlaI	GCGC	2 cut(s)	69, 280
GluI	GCNGC	4 cut(s)	300, 348, 375, 678
GsuI	CTGGAG	1 cut(s)	122
HaeIII	GGCC	5 cut(s)	37, 239, 246, 572, 665
HapII	CCGG	1 cut(s)	623
HhaI	GCGC	2 cut(s)	70, 281
Hin1II	CATG	1 cut(s)	182
Hin6I	GCGC	2 cut(s)	68, 279
HinP1I	GCGC	2 cut(s)	68, 279
HinfI	GANTC	2 cut(s)	200, 253
HpaII	CCGG	1 cut(s)	623
HphI	GGTGA	1 cut(s)	194
Hpy166II	GTNNAC	1 cut(s)	154
Hpy188I	TCNGA	3 cut(s)	63, 265, 512
Hpy188III	TCNNGA	2 cut(s)	101, 545
Hpy8I	GTNNAC	1 cut(s)	154
Hpy99I	CGWCG	4 cut(s)	17, 253, 311, 331
HpyAV	CCTTC	6 cut(s)	13, 270, 347, 364, 389, 604
HpyCH4IV	ACGT	4 cut(s)	15, 48, 110, 306
HpyCH4V	TGCA	5 cut(s)	287, 392, 485, 596, 680
HpyF10VI	GCNNNNNNNGC	6 cut(s)	65, 371, 578, 602, 647, 683
HpyF3I	CTNAG	6 cut(s)	241, 332, 509, 582, 642, 651
HpySE526I	ACGT	4 cut(s)	15, 48, 110, 306
Hsp92II	CATG	1 cut(s)	182
HspAI	GCGC	2 cut(s)	68, 279
KpnI	GGTACC	1 cut(s)	475
Kzo9I	GATC	3 cut(s)	274, 558, 598
LguI	GCTCTTC	1 cut(s)	2
LmnI	GCTCC	1 cut(s)	29
Lsp1109I	GCAGC	3 cut(s)	286, 386, 664
MaeII	ACGT	4 cut(s)	15, 48, 110, 306
MaeIII	GTNAC	1 cut(s)	514
MalI	GATC	3 cut(s)	276, 560, 600
MboI	GATC	3 cut(s)	274, 558, 598
MboII	GAAGA	3 cut(s)	19, 125, 442
MflI	RGATCY	2 cut(s)	558, 598
MhlI	GDGCHC	2 cut(s)	44, 61
MlsI	TGGCCA	2 cut(s)	572, 665
MluCI	AATT	2 cut(s)	210, 465
MluNI	TGGCCA	2 cut(s)	572, 665
MlyI	GAGTC	1 cut(s)	209
MmeI	TCCRAC	1 cut(s)	252
MnlI	CCTC	8 cut(s)	48, 97, 223, 250, 329, 355, 406, 637
Mox20I	TGGCCA	2 cut(s)	572, 665
MroXI	GAANNNNTTC	1 cut(s)	420
MscI	TGGCCA	2 cut(s)	572, 665
MslI	CAYNNNNRTG	2 cut(s)	212, 490
Msp20I	TGGCCA	2 cut(s)	572, 665
MspA1I	CMGCKG	1 cut(s)	350
MspI	CCGG	1 cut(s)	623
MspR9I	CCNGG	2 cut(s)	476, 564
Mva1269I	GAATGC	1 cut(s)	485
MvaI	CCWGG	2 cut(s)	476, 564
MvnI	CGCG	1 cut(s)	279
MwoI	GCNNNNNNNGC	6 cut(s)	65, 371, 578, 602, 647, 683
NdeII	GATC	3 cut(s)	274, 558, 598
NlaIII	CATG	1 cut(s)	182
NlaIV	GGNNCC	2 cut(s)	346, 473
NmeAIII	GCCGAG	1 cut(s)	63
NmuCI	GTSAC	1 cut(s)	514
PaqCI	CACCTGC	1 cut(s)	671
PasI	CCCWGGG	1 cut(s)	563
PciSI	GCTCTTC	1 cut(s)	2
PctI	GAATGC	1 cut(s)	485
PdmI	GAANNNNTTC	1 cut(s)	420
PfeI	GAWTC	1 cut(s)	253
PkrI	GCNGC	4 cut(s)	301, 349, 376, 679
PleI	GAGTC	1 cut(s)	208
PpsI	GAGTC	1 cut(s)	208
Ppu21I	YACGTR	1 cut(s)	49
Psp6I	CCWGG	2 cut(s)	474, 562
PspGI	CCWGG	2 cut(s)	474, 562
PspN4I	GGNNCC	2 cut(s)	346, 473
PspPI	GGNCC	2 cut(s)	237, 245
PstI	CTGCAG	1 cut(s)	682
PstNI	CAGNNNCTG	1 cut(s)	602
PsuI	RGATCY	2 cut(s)	558, 598
RsaI	GTAC	2 cut(s)	221, 473
RsaNI	GTAC	2 cut(s)	220, 472
RseI	CAYNNNNRTG	2 cut(s)	212, 490
SapI	GCTCTTC	1 cut(s)	2
SatI	GCNGC	4 cut(s)	300, 348, 375, 678
Sau3AI	GATC	3 cut(s)	274, 558, 598
Sau96I	GGNCC	2 cut(s)	237, 245
SchI	GAGTC	1 cut(s)	209
ScrFI	CCNGG	2 cut(s)	476, 564
SduI	GDGCHC	2 cut(s)	44, 61
SfcI	CTRYAG	1 cut(s)	678
SmiMI	CAYNNNNRTG	2 cut(s)	212, 490
SmlI	CTYRAG	1 cut(s)	101
SmoI	CTYRAG	1 cut(s)	101
Sse9I	AATT	2 cut(s)	210, 465
SsiI	CCGC	1 cut(s)	348
StyD4I	CCNGG	2 cut(s)	474, 562
TaiI	ACGT	4 cut(s)	18, 51, 113, 309
TaqI	TCGA	4 cut(s)	225, 251, 309, 381
TasI	AATT	2 cut(s)	210, 465
TatI	WGTACW	1 cut(s)	219
TauI	GCSGC	1 cut(s)	350
TfiI	GAWTC	1 cut(s)	253
TscAI	CASTG	3 cut(s)	90, 460, 598
TseFI	GTSAC	1 cut(s)	514
TseI	GCWGC	3 cut(s)	299, 374, 677
Tsp45I	GTSAC	1 cut(s)	514
TspDTI	ATGAA	2 cut(s)	167, 542
TspGWI	ACGGA	1 cut(s)	315
TspRI	CASTG	3 cut(s)	90, 460, 598
XmnI	GAANNNNTTC	1 cut(s)	420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.