Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004653

Physical Location & Seq

Genomic Coordinates Reverse (-)

5760481 .. 5761411

Gene Structure 931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1082200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 423 bp

ATGGCGAAGAGCGACGTGAAGGTTTTGGGAGCGTGGCCGAGCCCATACGCGATGAGGGCTTGGATCGCGCTCAATATCAAGTCAGTGGAGTATGAGCTTCTTGAGGAGACGTTCGAAGCCAAAAGCCAGCTTCTTCTCCAGTCCAATCCAGTCCACAAGAAAATCCCAGTTCTCATTCATTGTGATAAGTCCGTCTGTGAGTCTCTCATCATTGTGGAGTACATCGATGAGGTTTGGGCCTCAGGCCCGTCGATTCTCCCTTCTGACCCGTTGGATCGCGCTACTGCAAGATTTTGGGCTGCCTACGTCGATGAGAAGGTGCGCTTTTTTTTTGGTCAAATTGTAGGTGCGCCTTTTTTTTGGTCAAATTGTAGGTGTGTTTTTTTTTTGGGTCAAATTATAGGTGCGTTTGATTATCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009314 GO:0009404 GO:0009407 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009636 GO:0009639 GO:0009640 GO:0009651 GO:0009704 GO:0009719 GO:0009725 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010243 GO:0016740 GO:0016765 GO:0019748 GO:0022622 GO:0032501 GO:0032502 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043434 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048364 GO:0048519 GO:0048527 GO:0048528 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051186 GO:0060416 GO:0065007 GO:0071704 GO:0080134 GO:0080148 GO:0080167 GO:0090696 GO:0098754 GO:0099402 GO:1901564 GO:1901652 GO:1901698 GO:1901700 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_C_2 GST_C_3 GST_N GST_N_3

Protein Analysis

141

Amino Acids

15.92

Weight (kDa)

5.34

Isoelectric Point (pI)

34.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	5 - 77	1.6e-17	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	14 - 77	5e-12	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	14 - 77	1.3e-10	Glutathione S-transferase, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0004653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10360 AT1G10370
fragaria_vesca	FvH4_4g35560 FvH4_4g35600
malus_domestica	MD13G1081900.v1.1 MD13G1082000.v1.1 MD13G1082100.v1.1 MD13G1082200.v1.1 MD16G1081100.v1.1
prunus_persica	Prupe.1G264700_v2.0.a1 Prupe.1G264900_v2.0.a1
pyrus_communis	pycom13g07080 pycom16g07010
rosa_chinensis	RchiOBHm_Chr4g0445201 RchiOBHm_Chr5g0060741
rosa_laevigata	RLG00000005757 RLG00000035391
rosa_multiflora	Rmu_co8192854.1_g000001 Rmu_sc0000050.1_g000034 Rmu_sc0006568.1_g000003 Rmu_sc0029476.1_g000002
rosa_roxburghii	Rroxscaffold_5G00385720
rosa_rugosa	Rorug04G0360000 Rorug05G0333500
rosa_samantha	Rh4AG420800 Rh4BG432700 Rh4CG447400 Rh4DG428800 Rh5BG408500 Rh5DG423700
rosa_wichuraiana	Rw4G036090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	50, 68, 279
AclWI	GGATC	2 cut(s)	71, 282
AcoI	YGGCCR	1 cut(s)	35
AfaI	GTAC	1 cut(s)	221
AjiI	CACGTC	1 cut(s)	16
AluBI	AGCT	2 cut(s)	97, 130
AluI	AGCT	2 cut(s)	97, 130
Alw26I	GTCTC	2 cut(s)	101, 207
AlwI	GGATC	2 cut(s)	71, 282
AoxI	GGCC	3 cut(s)	35, 237, 244
ApeKI	GCWGC	1 cut(s)	299
AspLEI	GCGC	4 cut(s)	70, 281, 324, 352
AspS9I	GGNCC	2 cut(s)	237, 245
AsuII	TTCGAA	1 cut(s)	114
AxyI	CCTNAGG	1 cut(s)	241
BanII	GRGCYC	1 cut(s)	44
BbvI	GCAGC	1 cut(s)	286
BcoDI	GTCTC	2 cut(s)	101, 207
BisI	GCNGC	1 cut(s)	300
BlsI	GCNGC	1 cut(s)	301
BmgBI	CACGTC	1 cut(s)	16
BmgT120I	GGNCC	2 cut(s)	237, 245
BmrI	ACTGGG	1 cut(s)	161
BmuI	ACTGGG	1 cut(s)	161
BpmI	CTGGAG	1 cut(s)	122
Bpu14I	TTCGAA	1 cut(s)	114
BpuEI	CTTGAG	1 cut(s)	122
Bsa29I	ATCGAT	1 cut(s)	225
Bse1I	ACTGG	3 cut(s)	139, 149, 167
Bse21I	CCTNAGG	1 cut(s)	241
BseCI	ATCGAT	1 cut(s)	225
BseMII	CTCAG	1 cut(s)	255
BseNI	ACTGG	3 cut(s)	139, 149, 167
BseRI	GAGGAG	1 cut(s)	119
BseXI	GCAGC	1 cut(s)	286
Bsh1236I	CGCG	3 cut(s)	50, 68, 279
BshFI	GGCC	3 cut(s)	37, 239, 246
BshVI	ATCGAT	1 cut(s)	225
BsmAI	GTCTC	2 cut(s)	101, 207
BsmBI	CGTCTC	1 cut(s)	101
BsnI	GGCC	3 cut(s)	37, 239, 246
Bsp119I	TTCGAA	1 cut(s)	114
Bsp1286I	GDGCHC	1 cut(s)	44
Bsp143I	GATC	2 cut(s)	63, 274
BspANI	GGCC	3 cut(s)	37, 239, 246
BspCNI	CTCAG	1 cut(s)	254
BspDI	ATCGAT	1 cut(s)	225
BspFNI	CGCG	3 cut(s)	50, 68, 279
BspPI	GGATC	2 cut(s)	71, 282
BspQI	GCTCTTC	1 cut(s)	2
BspT104I	TTCGAA	1 cut(s)	114
BsrI	ACTGG	3 cut(s)	139, 149, 167
BssMI	GATC	2 cut(s)	63, 274
Bst6I	CTCTTC	1 cut(s)	2
BstBI	TTCGAA	1 cut(s)	114
BstC8I	GCNNGC	1 cut(s)	128
BstDEI	CTNAG	1 cut(s)	241
BstFNI	CGCG	3 cut(s)	50, 68, 279
BstHHI	GCGC	4 cut(s)	70, 281, 324, 352
BstKTI	GATC	2 cut(s)	66, 277
BstMAI	GTCTC	2 cut(s)	101, 207
BstMBI	GATC	2 cut(s)	63, 274
BstMWI	GCNNNNNNNGC	2 cut(s)	56, 65
BstUI	CGCG	3 cut(s)	50, 68, 279
BstV1I	GCAGC	1 cut(s)	286
Bsu15I	ATCGAT	1 cut(s)	225
Bsu36I	CCTNAGG	1 cut(s)	241
BsuRI	GGCC	3 cut(s)	37, 239, 246
BsuTUI	ATCGAT	1 cut(s)	225
BtgZI	GCGATG	1 cut(s)	65
BtrI	CACGTC	1 cut(s)	16
BtsIMutI	CAGTG	1 cut(s)	90
Cac8I	GCNNGC	1 cut(s)	128
CfoI	GCGC	4 cut(s)	70, 281, 324, 352
Cfr13I	GGNCC	2 cut(s)	237, 245
ClaI	ATCGAT	1 cut(s)	225
Csp6I	GTAC	1 cut(s)	220
CviQI	GTAC	1 cut(s)	220
DdeI	CTNAG	1 cut(s)	241
DpnI	GATC	2 cut(s)	65, 276
DpnII	GATC	2 cut(s)	63, 274
EaeI	YGGCCR	1 cut(s)	35
Eam1104I	CTCTTC	1 cut(s)	2
EarI	CTCTTC	1 cut(s)	2
Eco24I	GRGCYC	1 cut(s)	44
Eco81I	CCTNAGG	1 cut(s)	241
EcoT38I	GRGCYC	1 cut(s)	44
Esp3I	CGTCTC	1 cut(s)	101
FaiI	YATR	4 cut(s)	46, 93, 401, 421
FalI	AAGNNNNNCTT	2 cut(s)	308, 340
Fnu4HI	GCNGC	1 cut(s)	300
FriOI	GRGCYC	1 cut(s)	44
Fsp4HI	GCNGC	1 cut(s)	300
GlaI	GCGC	4 cut(s)	69, 280, 323, 351
GluI	GCNGC	1 cut(s)	300
GsuI	CTGGAG	1 cut(s)	122
HaeIII	GGCC	3 cut(s)	37, 239, 246
HhaI	GCGC	4 cut(s)	70, 281, 324, 352
Hin6I	GCGC	4 cut(s)	68, 279, 322, 350
HinP1I	GCGC	4 cut(s)	68, 279, 322, 350
HinfI	GANTC	2 cut(s)	200, 253
Hpy166II	GTNNAC	1 cut(s)	154
Hpy188I	TCNGA	1 cut(s)	265
Hpy188III	TCNNGA	1 cut(s)	101
Hpy8I	GTNNAC	1 cut(s)	154
Hpy99I	CGWCG	3 cut(s)	17, 253, 311
HpyAV	CCTTC	3 cut(s)	13, 270, 310
HpyCH4IV	ACGT	3 cut(s)	15, 110, 306
HpyCH4V	TGCA	1 cut(s)	287
HpyF10VI	GCNNNNNNNGC	2 cut(s)	56, 65
HpyF3I	CTNAG	1 cut(s)	241
HpySE526I	ACGT	3 cut(s)	15, 110, 306
HspAI	GCGC	4 cut(s)	68, 279, 322, 350
Kzo9I	GATC	2 cut(s)	63, 274
LguI	GCTCTTC	1 cut(s)	2
LmnI	GCTCC	1 cut(s)	29
LpnPI	CCDG	5 cut(s)	140, 152, 162, 180, 228
Lsp1109I	GCAGC	1 cut(s)	286
MaeII	ACGT	3 cut(s)	15, 110, 306
MalI	GATC	2 cut(s)	65, 276
MboI	GATC	2 cut(s)	63, 274
MboII	GAAGA	2 cut(s)	19, 125
MhlI	GDGCHC	1 cut(s)	44
MluCI	AATT	3 cut(s)	339, 367, 396
MlyI	GAGTC	1 cut(s)	209
MmeI	TCCRAC	1 cut(s)	252
MnlI	CCTC	4 cut(s)	48, 97, 223, 250
MslI	CAYNNNNRTG	1 cut(s)	212
MvnI	CGCG	3 cut(s)	50, 68, 279
MwoI	GCNNNNNNNGC	2 cut(s)	56, 65
NdeII	GATC	2 cut(s)	63, 274
NmeAIII	GCCGAG	1 cut(s)	63
NspV	TTCGAA	1 cut(s)	114
PciSI	GCTCTTC	1 cut(s)	2
PfeI	GAWTC	1 cut(s)	253
PkrI	GCNGC	1 cut(s)	301
PleI	GAGTC	1 cut(s)	208
PpsI	GAGTC	1 cut(s)	208
PspPI	GGNCC	2 cut(s)	237, 245
RsaI	GTAC	1 cut(s)	221
RsaNI	GTAC	1 cut(s)	220
RseI	CAYNNNNRTG	1 cut(s)	212
SapI	GCTCTTC	1 cut(s)	2
SatI	GCNGC	1 cut(s)	300
Sau3AI	GATC	2 cut(s)	63, 274
Sau96I	GGNCC	2 cut(s)	237, 245
SchI	GAGTC	1 cut(s)	209
SduI	GDGCHC	1 cut(s)	44
SfuI	TTCGAA	1 cut(s)	114
SmiMI	CAYNNNNRTG	1 cut(s)	212
SmlI	CTYRAG	1 cut(s)	101
SmoI	CTYRAG	1 cut(s)	101
Sse9I	AATT	3 cut(s)	339, 367, 396
TaiI	ACGT	3 cut(s)	18, 113, 309
TaqI	TCGA	4 cut(s)	114, 225, 251, 309
TasI	AATT	3 cut(s)	339, 367, 396
TatI	WGTACW	1 cut(s)	219
TfiI	GAWTC	1 cut(s)	253
TscAI	CASTG	1 cut(s)	90
TseI	GCWGC	1 cut(s)	299
TspDTI	ATGAA	1 cut(s)	167
TspGWI	ACGGA	1 cut(s)	181
TspRI	CASTG	1 cut(s)	90

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.