Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004653

Physical Location & Seq

Genomic Coordinates Reverse (-)

5706295 .. 5707923

Gene Structure 1629 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1081100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 696 bp

ATGGCGAAGAGCAACGTGAAGGTTTTGGGAGCGTGGCCGAGCCCATACGTGATGAGGGCTCGGGTCGCGCTCAATATCAAGTCGGTGGAGTATGAGTTTCTGGAGGAGACATTCGGATCCAAAAGCCGGCTTCTGCTCCAGTCCAATCCCGTCCACAAGAAAATCCCAGTTCTGATTCATGGCGACAAGCCCGTTTGTGAATCTCTCATCATTGTTGAGTACATCGATGAGGTTTGGTCCTCTGGCCCATCTATCCTCCCTTCTGACCCGTTCGATCGCGCTACTGCAAGATTCTGGGCTGCCTATATCGATGAGAAGTGGTTTCCGGCAATGAAAGGCATTGGTGCTGCTCAAGGAGATGATGCAAGGAAGGCAGCAGTCGAGCAAGTTGCCGAGGGGCTAGCTCAGTTGGAGGAAGCCTTTCAAAAAACCAGCAAAGGCAAGGACTTCTTTAGTGGAGACAAAATTGGGTACCTAGACATTGCGTTCGGGTGCTTCTTGGGGTGGCTCAGAGTCACAGAGAAAATGAATGGGGTCAAACTTCTGGACGGCGCAAAGATCCCAGGGCTGGTCACATGGGCTGAGAAGTTCAGTGCAGATCCTGCCGTGAAGGATGTCATGCCGGAGACTGACAAGCTGGCTGAGTTTGCTAAGATTCTTGCGGCCAAAATGAGAGGTGCAGGGGCTCCCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009314 GO:0009404 GO:0009407 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009636 GO:0009639 GO:0009640 GO:0009651 GO:0009704 GO:0009719 GO:0009725 GO:0009791 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010243 GO:0016740 GO:0016765 GO:0019748 GO:0022622 GO:0032501 GO:0032502 GO:0034641 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043434 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048364 GO:0048519 GO:0048527 GO:0048528 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051186 GO:0060416 GO:0065007 GO:0071704 GO:0080134 GO:0080148 GO:0080167 GO:0090696 GO:0098754 GO:0099402 GO:1901564 GO:1901652 GO:1901698 GO:1901700 GO:2000070

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_C_2 GST_C_3 GST_N GST_N_3

Protein Analysis

232

Amino Acids

25.37

Weight (kDa)

7.68

Isoelectric Point (pI)

30.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	5 - 77	1.5e-18	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	14 - 77	8.7e-13	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	14 - 77	1.9e-10	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	98 - 201	1e-08	Glutathione S-transferase, C-terminal domain
GST_C_7	PF22041	120 - 175	3e-07	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	121 - 193	3.6e-09	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10360 AT1G10370
fragaria_vesca	FvH4_4g35560 FvH4_4g35600
malus_domestica	MD13G1081900.v1.1 MD13G1082000.v1.1 MD13G1082100.v1.1 MD13G1082200.v1.1 MD16G1081100.v1.1
prunus_persica	Prupe.1G264700_v2.0.a1 Prupe.1G264900_v2.0.a1
pyrus_communis	pycom13g07080 pycom16g07010
rosa_chinensis	RchiOBHm_Chr4g0445201 RchiOBHm_Chr5g0060741
rosa_laevigata	RLG00000005757 RLG00000035391
rosa_multiflora	Rmu_co8192854.1_g000001 Rmu_sc0000050.1_g000034 Rmu_sc0006568.1_g000003 Rmu_sc0029476.1_g000002
rosa_roxburghii	Rroxscaffold_5G00385720
rosa_rugosa	Rorug04G0360000 Rorug05G0333500
rosa_samantha	Rh4AG420800 Rh4BG432700 Rh4CG447400 Rh4DG428800 Rh5BG408500 Rh5DG423700
rosa_wichuraiana	Rw4G036090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	471
AccB1I	GGYRCC	1 cut(s)	471
AccII	CGCG	2 cut(s)	68, 279
AciI	CCGC	1 cut(s)	662
AclWI	GGATC	4 cut(s)	111, 124, 553, 593
AcoI	YGGCCR	2 cut(s)	35, 663
AfaI	GTAC	2 cut(s)	221, 473
AfiI	CCNNNNNNNGG	2 cut(s)	126, 568
AgsI	TTSAA	1 cut(s)	425
AjnI	CCWGG	1 cut(s)	562
AluBI	AGCT	2 cut(s)	404, 637
AluI	AGCT	2 cut(s)	404, 637
Alw26I	GTCTC	3 cut(s)	101, 453, 620
AlwI	GGATC	4 cut(s)	111, 124, 553, 593
AlwNI	CAGNNNCTG	1 cut(s)	602
Ama87I	CYCGRG	1 cut(s)	60
AoxI	GGCC	3 cut(s)	35, 244, 663
ApeKI	GCWGC	3 cut(s)	299, 347, 374
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
Asp700I	GAANNNNTTC	1 cut(s)	420
Asp718I	GGTACC	1 cut(s)	471
AspLEI	GCGC	3 cut(s)	70, 281, 554
AspS9I	GGNCC	2 cut(s)	237, 245
AsuNHI	GCTAGC	1 cut(s)	400
AvaI	CYCGRG	1 cut(s)	60
AvaII	GGWCC	1 cut(s)	237
BamHI	GGATCC	1 cut(s)	116
BanI	GGYRCC	1 cut(s)	471
BanII	GRGCYC	3 cut(s)	44, 61, 688
BbvI	GCAGC	3 cut(s)	286, 334, 386
BccI	CCATC	1 cut(s)	256
BceAI	ACGGC	2 cut(s)	565, 590
BcgI	CGANNNNNNTGC	2 cut(s)	371, 405
BciT130I	CCWGG	1 cut(s)	564
BcoDI	GTCTC	3 cut(s)	101, 453, 620
BfaI	CTAG	2 cut(s)	401, 476
BisI	GCNGC	4 cut(s)	300, 348, 375, 663
BlsI	GCNGC	4 cut(s)	301, 349, 376, 664
Bme1390I	CCNGG	1 cut(s)	564
Bme18I	GGWCC	1 cut(s)	237
BmeT110I	CYCGRG	1 cut(s)	60
BmgT120I	GGNCC	2 cut(s)	237, 245
BmiI	GGNNCC	3 cut(s)	118, 473, 687
BmrFI	CCNGG	1 cut(s)	564
BmrI	ACTGGG	1 cut(s)	161
BmsI	GCATC	1 cut(s)	352
BmtI	GCTAGC	1 cut(s)	404
BmuI	ACTGGG	1 cut(s)	161
BpmI	CTGGAG	2 cut(s)	122, 122
BpuEI	CTTGAG	1 cut(s)	336
Bsa29I	ATCGAT	2 cut(s)	225, 309
BsaAI	YACGTR	1 cut(s)	49
BsaJI	CCNNGG	3 cut(s)	393, 562, 563
BsaXI	ACNNNNNCTCC	2 cut(s)	80, 110
Bsc4I	CCNNNNNNNGG	2 cut(s)	126, 568
Bse118I	RCCGGY	1 cut(s)	126
Bse1I	ACTGG	2 cut(s)	139, 167
Bse3DI	GCAATG	2 cut(s)	336, 480
BseBI	CCWGG	1 cut(s)	564
BseCI	ATCGAT	2 cut(s)	225, 309
BseDI	CCNNGG	3 cut(s)	393, 562, 563
BseGI	GGATG	1 cut(s)	619
BseLI	CCNNNNNNNGG	2 cut(s)	126, 568
BseMI	GCAATG	2 cut(s)	336, 480
BseMII	CTCAG	4 cut(s)	419, 523, 573, 633
BseNI	ACTGG	2 cut(s)	139, 167
BseRI	GAGGAG	1 cut(s)	119
BseXI	GCAGC	3 cut(s)	286, 334, 386
BsgI	GTGCAG	1 cut(s)	615
Bsh1236I	CGCG	2 cut(s)	68, 279
Bsh1285I	CGRYCG	1 cut(s)	277
BshFI	GGCC	3 cut(s)	37, 246, 665
BshNI	GGYRCC	1 cut(s)	471
BshVI	ATCGAT	2 cut(s)	225, 309
BsiEI	CGRYCG	1 cut(s)	277
BsiHKCI	CYCGRG	1 cut(s)	60
BsiSI	CCGG	3 cut(s)	127, 326, 623
BslI	CCNNNNNNNGG	2 cut(s)	126, 568
BsmAI	GTCTC	3 cut(s)	101, 453, 620
BsnI	GGCC	3 cut(s)	37, 246, 665
BsoBI	CYCGRG	1 cut(s)	60
Bsp1286I	GDGCHC	3 cut(s)	44, 61, 688
Bsp143I	GATC	4 cut(s)	116, 274, 558, 598
BspACI	CCGC	1 cut(s)	662
BspANI	GGCC	3 cut(s)	37, 246, 665
BspCNI	CTCAG	4 cut(s)	418, 522, 574, 634
BspDI	ATCGAT	2 cut(s)	225, 309
BspFNI	CGCG	2 cut(s)	68, 279
BspLI	GGNNCC	3 cut(s)	118, 473, 687
BspOI	GCTAGC	1 cut(s)	404
BspPI	GGATC	4 cut(s)	111, 124, 553, 593
BspQI	GCTCTTC	1 cut(s)	2
BspT107I	GGYRCC	1 cut(s)	471
BsrDI	GCAATG	2 cut(s)	336, 480
BsrFI	RCCGGY	1 cut(s)	126
BsrI	ACTGG	2 cut(s)	139, 167
BssAI	RCCGGY	1 cut(s)	126
BssECI	CCNNGG	3 cut(s)	393, 562, 563
BssMI	GATC	4 cut(s)	116, 274, 558, 598
Bst2UI	CCWGG	1 cut(s)	564
Bst6I	CTCTTC	1 cut(s)	2
BstAPI	GCANNNNNTGC	1 cut(s)	602
BstBAI	YACGTR	1 cut(s)	49
BstC8I	GCNNGC	3 cut(s)	128, 402, 639
BstDEI	CTNAG	5 cut(s)	405, 509, 582, 642, 651
BstF5I	GGATG	1 cut(s)	619
BstFNI	CGCG	2 cut(s)	68, 279
BstHHI	GCGC	3 cut(s)	70, 281, 554
BstKTI	GATC	4 cut(s)	119, 277, 561, 601
BstMAI	GTCTC	3 cut(s)	101, 453, 620
BstMBI	GATC	4 cut(s)	116, 274, 558, 598
BstMCI	CGRYCG	1 cut(s)	277
BstMWI	GCNNNNNNNGC	4 cut(s)	65, 371, 602, 647
BstNI	CCWGG	1 cut(s)	564
BstSCI	CCNGG	1 cut(s)	562
BstUI	CGCG	2 cut(s)	68, 279
BstV1I	GCAGC	3 cut(s)	286, 334, 386
BstX2I	RGATCY	3 cut(s)	116, 558, 598
BstYI	RGATCY	3 cut(s)	116, 558, 598
Bsu15I	ATCGAT	2 cut(s)	225, 309
BsuRI	GGCC	3 cut(s)	37, 246, 665
BsuTUI	ATCGAT	2 cut(s)	225, 309
BtsCI	GGATG	1 cut(s)	619
BtsIMutI	CAGTG	1 cut(s)	598
Cac8I	GCNNGC	3 cut(s)	128, 402, 639
CaiI	CAGNNNCTG	1 cut(s)	602
CfoI	GCGC	3 cut(s)	70, 281, 554
Cfr10I	RCCGGY	1 cut(s)	126
Cfr13I	GGNCC	2 cut(s)	237, 245
ClaI	ATCGAT	2 cut(s)	225, 309
Csp6I	GTAC	2 cut(s)	220, 472
CviAII	CATG	3 cut(s)	179, 576, 619
CviQI	GTAC	2 cut(s)	220, 472
DdeI	CTNAG	5 cut(s)	405, 509, 582, 642, 651
DpnI	GATC	4 cut(s)	118, 276, 560, 600
DpnII	GATC	4 cut(s)	116, 274, 558, 598
EaeI	YGGCCR	2 cut(s)	35, 663
Eam1104I	CTCTTC	1 cut(s)	2
EarI	CTCTTC	1 cut(s)	2
Eco24I	GRGCYC	3 cut(s)	44, 61, 688
Eco47I	GGWCC	1 cut(s)	237
Eco88I	CYCGRG	1 cut(s)	60
EcoRII	CCWGG	1 cut(s)	562
EcoT38I	GRGCYC	3 cut(s)	44, 61, 688
FaeI	CATG	3 cut(s)	182, 579, 622
FaiI	YATR	6 cut(s)	46, 93, 180, 306, 577, 620
FalI	AAGNNNNNCTT	2 cut(s)	434, 466
FatI	CATG	3 cut(s)	178, 575, 618
Fnu4HI	GCNGC	4 cut(s)	300, 348, 375, 663
FokI	GGATG	1 cut(s)	626
FriOI	GRGCYC	3 cut(s)	44, 61, 688
Fsp4HI	GCNGC	4 cut(s)	300, 348, 375, 663
FspBI	CTAG	2 cut(s)	401, 476
GlaI	GCGC	3 cut(s)	69, 280, 553
GluI	GCNGC	4 cut(s)	300, 348, 375, 663
GsuI	CTGGAG	2 cut(s)	122, 122
HaeIII	GGCC	3 cut(s)	37, 246, 665
HapII	CCGG	3 cut(s)	127, 326, 623
HhaI	GCGC	3 cut(s)	70, 281, 554
Hin1II	CATG	3 cut(s)	182, 579, 622
Hin6I	GCGC	3 cut(s)	68, 279, 552
HinP1I	GCGC	3 cut(s)	68, 279, 552
HinfI	GANTC	5 cut(s)	175, 200, 291, 513, 655
HpaII	CCGG	3 cut(s)	127, 326, 623
Hpy166II	GTNNAC	1 cut(s)	154
Hpy188I	TCNGA	4 cut(s)	116, 174, 265, 512
Hpy188III	TCNNGA	2 cut(s)	101, 545
Hpy8I	GTNNAC	1 cut(s)	154
HpyAV	CCTTC	4 cut(s)	13, 270, 364, 604
HpyCH4IV	ACGT	2 cut(s)	15, 48
HpyCH4V	TGCA	4 cut(s)	287, 365, 596, 680
HpyF10VI	GCNNNNNNNGC	4 cut(s)	65, 371, 602, 647
HpyF3I	CTNAG	5 cut(s)	405, 509, 582, 642, 651
HpySE526I	ACGT	2 cut(s)	15, 48
Hsp92II	CATG	3 cut(s)	182, 579, 622
HspAI	GCGC	3 cut(s)	68, 279, 552
KpnI	GGTACC	1 cut(s)	475
KroI	GCCGGC	1 cut(s)	126
KroNI	GCCGGC	1 cut(s)	128
Kzo9I	GATC	4 cut(s)	116, 274, 558, 598
LguI	GCTCTTC	1 cut(s)	2
LmnI	GCTCC	3 cut(s)	29, 141, 691
Lsp1109I	GCAGC	3 cut(s)	286, 334, 386
LweI	GCATC	1 cut(s)	352
MaeI	CTAG	2 cut(s)	401, 476
MaeII	ACGT	2 cut(s)	15, 48
MaeIII	GTNAC	2 cut(s)	514, 571
MalI	GATC	4 cut(s)	118, 276, 560, 600
MboI	GATC	4 cut(s)	116, 274, 558, 598
MboII	GAAGA	1 cut(s)	19
MflI	RGATCY	3 cut(s)	116, 558, 598
MhlI	GDGCHC	3 cut(s)	44, 61, 688
MluCI	AATT	1 cut(s)	465
MlyI	GAGTC	1 cut(s)	522
MmeI	TCCRAC	1 cut(s)	390
MnlI	CCTC	8 cut(s)	48, 97, 223, 250, 266, 388, 406, 668
MroNI	GCCGGC	1 cut(s)	126
MroXI	GAANNNNTTC	1 cut(s)	420
MspI	CCGG	3 cut(s)	127, 326, 623
MspR9I	CCNGG	1 cut(s)	564
MvaI	CCWGG	1 cut(s)	564
MvnI	CGCG	2 cut(s)	68, 279
MwoI	GCNNNNNNNGC	4 cut(s)	65, 371, 602, 647
NaeI	GCCGGC	1 cut(s)	128
NdeII	GATC	4 cut(s)	116, 274, 558, 598
NgoMIV	GCCGGC	1 cut(s)	126
NheI	GCTAGC	1 cut(s)	400
NlaIII	CATG	3 cut(s)	182, 579, 622
NlaIV	GGNNCC	3 cut(s)	118, 473, 687
NmeAIII	GCCGAG	2 cut(s)	63, 418
NmuCI	GTSAC	2 cut(s)	514, 571
PasI	CCCWGGG	1 cut(s)	563
PciSI	GCTCTTC	1 cut(s)	2
PdiI	GCCGGC	1 cut(s)	128
PdmI	GAANNNNTTC	1 cut(s)	420
PfeI	GAWTC	4 cut(s)	175, 200, 291, 655
PkrI	GCNGC	4 cut(s)	301, 349, 376, 664
Ple19I	CGATCG	1 cut(s)	277
PleI	GAGTC	1 cut(s)	521
PpsI	GAGTC	1 cut(s)	521
Ppu21I	YACGTR	1 cut(s)	49
Psp6I	CCWGG	1 cut(s)	562
PspGI	CCWGG	1 cut(s)	562
PspN4I	GGNNCC	3 cut(s)	118, 473, 687
PspPI	GGNCC	2 cut(s)	237, 245
PstNI	CAGNNNCTG	1 cut(s)	602
PsuI	RGATCY	3 cut(s)	116, 558, 598
PvuI	CGATCG	1 cut(s)	277
RsaI	GTAC	2 cut(s)	221, 473
RsaNI	GTAC	2 cut(s)	220, 472
SapI	GCTCTTC	1 cut(s)	2
SatI	GCNGC	4 cut(s)	300, 348, 375, 663
Sau3AI	GATC	4 cut(s)	116, 274, 558, 598
Sau96I	GGNCC	2 cut(s)	237, 245
SchI	GAGTC	1 cut(s)	522
ScrFI	CCNGG	1 cut(s)	564
SduI	GDGCHC	3 cut(s)	44, 61, 688
SetI	ASST	8 cut(s)	18, 24, 51, 234, 406, 477, 639, 679
SfaNI	GCATC	1 cut(s)	352
SinI	GGWCC	1 cut(s)	237
SmlI	CTYRAG	1 cut(s)	351
SmoI	CTYRAG	1 cut(s)	351
Sse9I	AATT	1 cut(s)	465
SsiI	CCGC	1 cut(s)	662
SspMI	CTAG	2 cut(s)	401, 476
StyD4I	CCNGG	1 cut(s)	562
TaiI	ACGT	2 cut(s)	18, 51
TaqI	TCGA	4 cut(s)	225, 273, 309, 381
TasI	AATT	1 cut(s)	465
TatI	WGTACW	1 cut(s)	219
TauI	GCSGC	1 cut(s)	665
TfiI	GAWTC	4 cut(s)	175, 200, 291, 655
TscAI	CASTG	1 cut(s)	598
TseFI	GTSAC	2 cut(s)	514, 571
TseI	GCWGC	3 cut(s)	299, 347, 374
Tsp45I	GTSAC	2 cut(s)	514, 571
TspDTI	ATGAA	3 cut(s)	167, 347, 542
TspRI	CASTG	1 cut(s)	598
VpaK11BI	GGWCC	1 cut(s)	237
XmnI	GAANNNNTTC	1 cut(s)	420
XspI	CTAG	2 cut(s)	401, 476

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.