MD14G1140100.v1.1

caffeic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004266
Physical Location & Seq
Reverse (-)
23039919 .. 23040797
879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1140100.v1.1.491

Sequence Viewer

Length: 567 bp
ATGAATGTGGTGGATTACATTGGTAGGGATGACAGACTTGGTGGACTGTTTACGGACTCAATGAAAGAGTTTAACCTCATTTTTAAGAAGGAGATTCTGGAGATTTATACGGGTTTCGAAGGTCTACACACACTGGTAGATGTGGGTGGTGGCGACGGTACTATTCTTAACATGATAATTTCCAAGCACCCTGCCATCAAGGGCATCAACTATGATTTGCCTTCAGTTGTCGAAAAGTCTCCCTCCCATCCCGGTATAGAGCACATTGCAGGAGATATGTTTGTGAGGATTCCGAAAGGCGATGCCATTTTCATGAAGTGGATACTACACGGTTGGGATGATGATCGATGCTTGATGATACTGAAGAATTGCTATGAGGCCTTGCCAGACAACGGAAAAGTGATAGTGGTGGATATGGTGATACAAGAAGCCCCGGGAACAAGTTTTTCTGCTAAAAGTTTGTTTCTGTTTGATGTGTACCTAATGAATACCAATGTGATGGGAAAGGAGAGAACAGAGAGAGAATTACAAAGTATGGCAAAAGCAGCAGGATTTTCAGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

189

Amino Acids

20.98

Weight (kDa)

5.07

Isoelectric Point (pI)

26.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 4 - 186 1.4e-52 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 124
AcuI CTGAAG 2 cut(s) 207, 383
AfaI GTAC 2 cut(s) 160, 479
AfiI CCNNNNNNNGG 1 cut(s) 392
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 243
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 1 cut(s) 545
AsuC2I CCSGG 3 cut(s) 252, 434, 435
AsuHPI GGTGA 1 cut(s) 430
AsuII TTCGAA 1 cut(s) 117
AvaI CYCGRG 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 557
BccI CCATC 3 cut(s) 203, 255, 493
BciVI GTATCC 1 cut(s) 315
BcnI CCSGG 3 cut(s) 252, 434, 435
BcoDI GTCTC 1 cut(s) 243
BfuI GTATCC 1 cut(s) 315
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 3 cut(s) 252, 434, 435
BmeT110I CYCGRG 1 cut(s) 433
BmrFI CCNGG 3 cut(s) 252, 434, 435
BmsI GCATC 3 cut(s) 213, 292, 338
BpmI CTGGAG 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 117
BpuMI CCSGG 3 cut(s) 252, 434, 435
Bsa29I ATCGAT 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 432, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 346
BseDI CCNNGG 2 cut(s) 432, 433
BseGI GGATG 3 cut(s) 34, 247, 343
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 557
BshFI GGCC 1 cut(s) 380
BshVI ATCGAT 1 cut(s) 346
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 433
BsiSI CCGG 2 cut(s) 252, 434
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 433
Bsp119I TTCGAA 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 1 cut(s) 343
BspANI GGCC 1 cut(s) 380
BspDI ATCGAT 1 cut(s) 346
BspHI TCATGA 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 2 cut(s) 432, 433
BssMI GATC 1 cut(s) 343
Bst4CI ACNGT 3 cut(s) 48, 158, 332
BstBI TTCGAA 1 cut(s) 117
BstF5I GGATG 3 cut(s) 34, 247, 343
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstSCI CCNGG 3 cut(s) 250, 432, 433
BstV1I GCAGC 1 cut(s) 557
BstXI CCANNNNNNTGG 1 cut(s) 499
Bsu15I ATCGAT 1 cut(s) 346
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 1 cut(s) 380
BsuTUI ATCGAT 1 cut(s) 346
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 3 cut(s) 34, 247, 343
BtsIMutI CAGTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 312
Cfr9I CCCGGG 1 cut(s) 433
ClaI ATCGAT 1 cut(s) 346
Csp6I GTAC 2 cut(s) 159, 478
CviAII CATG 2 cut(s) 172, 313
CviJI RGCY 2 cut(s) 380, 431
CviKI_1 RGCY 2 cut(s) 380, 431
CviQI GTAC 2 cut(s) 159, 478
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
Eco147I AGGCCT 1 cut(s) 380
Eco57I CTGAAG 2 cut(s) 207, 383
Eco88I CYCGRG 1 cut(s) 433
FaeI CATG 2 cut(s) 175, 316
FaiI YATR 9 cut(s) 108, 173, 213, 257, 278, 314, 375, 416, 536
FatI CATG 2 cut(s) 171, 312
FblI GTMKAC 1 cut(s) 124
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 3 cut(s) 41, 234, 350
Fsp4HI GCNGC 1 cut(s) 546
GluI GCNGC 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 1 cut(s) 380
HapII CCGG 2 cut(s) 252, 434
Hin1II CATG 2 cut(s) 175, 316
HinfI GANTC 3 cut(s) 56, 94, 289
HpaII CCGG 2 cut(s) 252, 434
HphI GGTGA 1 cut(s) 430
Hpy166II GTNNAC 4 cut(s) 44, 51, 125, 478
Hpy188I TCNGA 2 cut(s) 294, 559
Hpy188III TCNNGA 2 cut(s) 98, 313
Hpy8I GTNNAC 4 cut(s) 44, 51, 125, 478
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 3 cut(s) 82, 113, 231
HpyCH4III ACNGT 3 cut(s) 48, 158, 332
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
Hsp92II CATG 2 cut(s) 175, 316
Kzo9I GATC 1 cut(s) 343
LpnPI CCDG 8 cut(s) 83, 119, 204, 255, 265, 399, 447, 534
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 3 cut(s) 213, 292, 338
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 3 cut(s) 177, 367, 524
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 4 cut(s) 86, 253, 279, 370
MseI TTAA 3 cut(s) 72, 84, 168
MslI CAYNNNNRTG 1 cut(s) 311
MspI CCGG 2 cut(s) 252, 434
MspR9I CCNGG 3 cut(s) 252, 434, 435
MwoI GCNNNNNNNGC 1 cut(s) 545
NciI CCSGG 3 cut(s) 252, 434, 435
NdeII GATC 1 cut(s) 343
NlaIII CATG 2 cut(s) 175, 316
NspV TTCGAA 1 cut(s) 117
PagI TCATGA 1 cut(s) 312
PceI AGGCCT 1 cut(s) 380
PfeI GAWTC 2 cut(s) 94, 289
PkrI GCNGC 1 cut(s) 547
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
RsaI GTAC 2 cut(s) 160, 479
RsaNI GTAC 2 cut(s) 159, 478
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 3 cut(s) 72, 84, 168
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 1 cut(s) 343
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 3 cut(s) 252, 434, 435
SduI GDGCHC 1 cut(s) 264
SetI ASST 3 cut(s) 78, 124, 483
SfaNI GCATC 3 cut(s) 213, 292, 338
SfuI TTCGAA 1 cut(s) 117
SmaI CCCGGG 1 cut(s) 435
SmiMI CAYNNNNRTG 1 cut(s) 311
Sse9I AATT 3 cut(s) 177, 367, 524
SseBI AGGCCT 1 cut(s) 380
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 250, 432, 433
TaaI ACNGT 3 cut(s) 48, 158, 332
TaqI TCGA 3 cut(s) 117, 231, 346
TasI AATT 3 cut(s) 177, 367, 524
TfiI GAWTC 2 cut(s) 94, 289
Tru1I TTAA 3 cut(s) 72, 84, 168
Tru9I TTAA 3 cut(s) 72, 84, 168
TscAI CASTG 1 cut(s) 138
TseI GCWGC 1 cut(s) 545
TspDTI ATGAA 5 cut(s) 17, 77, 301, 329, 500
TspGWI ACGGA 2 cut(s) 68, 408
TspMI CCCGGG 1 cut(s) 433
TspRI CASTG 1 cut(s) 138
XmaI CCCGGG 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.