MD14G1140300.v1.1

caffeic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004266
Physical Location & Seq
Reverse (-)
23045447 .. 23045785
339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1140300.v1.1.491

Sequence Viewer

Length: 339 bp
ATGTCCACAATTAATGGAGAAGAGGAAGCTGACTCCCAATATGCTTCGCAGTTGGCAAACGTATCAGTATTGCCTATGGTGCTTAAAGCAGCCATAGAGCTTGGTGTACTTGACATTATCCACAGAGCTGGCGCCGGAGCCCTGCTTTCGCCTTCCCAAATAGCGTCTCAGCTTCGATCTCTCCACAATCCGAATGCCTCTTCAGTTCTTGATCGCATGCTTTGCCTTCTTTCTGCTTACTCTATTCTCACTTGCTCAACAATCCAAGCTAATGGGAAGGTTATTAGGGTTTATGGGTTGGCACCTGTCTCCAAATACTTTATTCACGAGAAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

113

Amino Acids

11.91

Weight (kDa)

6.81

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 17 - 108 2.4e-18 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 131, 301
AcuI CTGAAG 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 108
AluBI AGCT 5 cut(s) 29, 100, 128, 172, 269
AluI AGCT 5 cut(s) 29, 100, 128, 172, 269
Alw26I GTCTC 2 cut(s) 171, 313
ApeKI GCWGC 1 cut(s) 89
AseI ATTAAT 1 cut(s) 12
AspLEI GCGC 1 cut(s) 134
BanI GGYRCC 2 cut(s) 131, 301
BanII GRGCYC 1 cut(s) 142
BauI CACGAG 1 cut(s) 326
BbvI GCAGC 1 cut(s) 101
BcoDI GTCTC 2 cut(s) 171, 313
BfoI RGCGCY 1 cut(s) 135
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
BmiI GGNNCC 3 cut(s) 133, 139, 303
BsaHI GRCGYC 1 cut(s) 132
BseMII CTCAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 101
BshNI GGYRCC 2 cut(s) 131, 301
BsiSI CCGG 1 cut(s) 135
BsmAI GTCTC 2 cut(s) 171, 313
BsmBI CGTCTC 1 cut(s) 171
BsmI GAATGC 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 2 cut(s) 176, 211
BspCNI CTCAG 1 cut(s) 181
BspLI GGNNCC 3 cut(s) 133, 139, 303
BspT107I GGYRCC 2 cut(s) 131, 301
BssMI GATC 2 cut(s) 176, 211
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 326
Bst2BI CACGAG 1 cut(s) 326
Bst6I CTCTTC 2 cut(s) 15, 205
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 130, 218
BstDEI CTNAG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 2 cut(s) 179, 214
BstMAI GTCTC 2 cut(s) 171, 313
BstMBI GATC 2 cut(s) 176, 211
BstMWI GCNNNNNNNGC 2 cut(s) 79, 222
BstNSI RCATGY 1 cut(s) 220
BstV1I GCAGC 1 cut(s) 101
BstXI CCANNNNNNTGG 2 cut(s) 128, 272
Cac8I GCNNGC 2 cut(s) 130, 218
CfoI GCGC 1 cut(s) 134
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 107
CviAII CATG 1 cut(s) 217
CviJI RGCY 7 cut(s) 29, 92, 100, 128, 140, 172, 269
CviKI_1 RGCY 7 cut(s) 29, 92, 100, 128, 140, 172, 269
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 1 cut(s) 168
DinI GGCGCC 1 cut(s) 133
DpnI GATC 2 cut(s) 178, 213
DpnII GATC 2 cut(s) 176, 211
Eam1104I CTCTTC 2 cut(s) 15, 205
EarI CTCTTC 2 cut(s) 15, 205
Eco24I GRGCYC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 142
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 1 cut(s) 220
FaiI YATR 5 cut(s) 42, 77, 95, 218, 294
FatI CATG 1 cut(s) 216
Fnu4HI GCNGC 1 cut(s) 90
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 1 cut(s) 90
GlaI GCGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 90
HaeII RGCGCY 1 cut(s) 135
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 1 cut(s) 32
HpaII CCGG 1 cut(s) 135
Hpy166II GTNNAC 2 cut(s) 6, 107
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 2 cut(s) 209, 326
Hpy8I GTNNAC 2 cut(s) 6, 107
HpyAV CCTTC 3 cut(s) 162, 236, 271
HpyCH4IV ACGT 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 222
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 1 cut(s) 60
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 132
KasI GGCGCC 1 cut(s) 131
Kzo9I GATC 2 cut(s) 176, 211
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 4 cut(s) 114, 148, 155, 318
Lsp1109I GCAGC 1 cut(s) 101
MaeII ACGT 1 cut(s) 60
MalI GATC 2 cut(s) 178, 213
MboI GATC 2 cut(s) 176, 211
MboII GAAGA 2 cut(s) 32, 192
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 1 cut(s) 9
Mly113I GGCGCC 1 cut(s) 132
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 2 cut(s) 16, 208
MseI TTAA 2 cut(s) 12, 84
MspI CCGG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 79, 222
NarI GGCGCC 1 cut(s) 132
NdeII GATC 2 cut(s) 176, 211
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 3 cut(s) 133, 139, 303
NspI RCATGY 1 cut(s) 220
PaeI GCATGC 1 cut(s) 220
PctI GAATGC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 91
PleI GAGTC 1 cut(s) 26
PluTI GGCGCC 1 cut(s) 135
PpsI GAGTC 1 cut(s) 26
PshBI ATTAAT 1 cut(s) 12
PspN4I GGNNCC 3 cut(s) 133, 139, 303
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 12, 84
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 2 cut(s) 176, 211
SchI GAGTC 1 cut(s) 26
SduI GDGCHC 1 cut(s) 142
SetI ASST 8 cut(s) 31, 63, 102, 130, 174, 271, 282, 307
SfoI GGCGCC 1 cut(s) 133
SphI GCATGC 1 cut(s) 220
Sse9I AATT 1 cut(s) 9
SspDI GGCGCC 1 cut(s) 131
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 175
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 106
Tru1I TTAA 2 cut(s) 12, 84
Tru9I TTAA 2 cut(s) 12, 84
TseI GCWGC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 12
XceI RCATGY 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.