MD14G1140600.v1.1

caffeic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004266
Physical Location & Seq
Reverse (-)
23070366 .. 23071714
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1140600.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCTTCGCCTTTTTTCCGCTTACTTTGTTCTCACTTGCTCGACAATCCAAGCTAATGGGAATGTTATTAGGATTTGTGGGTTTGCACCTGTCTCCAAGTACTTTATTAAGAACCAGGGTGGGGTGTCTTTGGCACCATTGCTCAATTTATGCCATGACAAGGCCTATGGTATGAATGTGGTGGATTACATTGATAGGGATGAAAGACTTGGTAATGTGTTCAAGGACTCAATGAAAGAGTTTAACCTCATTTTTAAGAAGGAGATTATGGAGATTTATACGAGTCTCGAAGGTTTACGTGCACTGGTAGATGTGGGTGGTGGCGATGGTACTATTCCTAACTATGATTTGCCTTCGGTTGTTGAAAAGTCTCCCTCCCATCCCGGTATAGAGCACATTGCAGGAGATATGTTTGTAAGGATTCCGAAAGGCGATGCCATTTTCATGAAGAATTGCTATGAGGCCTTGCCAGACAATGGAAAAGTGATAGTGGTGGATATGAGAATAAAGAGAGAATTACAAAGTTTGGCAAAAGCAGCAAGATTTTCAGACATTCGAGTTGCTTGTTCCACTTTTACCTTTTCCGTCGTGGAACTGTTAAAAATATGTAGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

206

Amino Acids

22.82

Weight (kDa)

7.57

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 51 - 172 3e-19 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 1 cut(s) 476
AciI CCGC 1 cut(s) 18
AfaI GTAC 2 cut(s) 101, 331
AfiI CCNNNNNNNGG 3 cut(s) 121, 160, 476
AgsI TTSAA 2 cut(s) 223, 365
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 1 cut(s) 53
AluI AGCT 1 cut(s) 53
Alw21I GWGCWC 2 cut(s) 304, 396
Alw26I GTCTC 3 cut(s) 97, 290, 375
Alw44I GTGCAC 1 cut(s) 300
AoxI GGCC 2 cut(s) 162, 462
ApaLI GTGCAC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 536
AsuC2I CCSGG 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 304
BanI GGYRCC 1 cut(s) 133
Bbv12I GWGCWC 2 cut(s) 304, 396
BbvI GCAGC 1 cut(s) 548
BccI CCATC 2 cut(s) 320, 387
BciT130I CCWGG 1 cut(s) 116
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 3 cut(s) 97, 290, 375
BfaI CTAG 1 cut(s) 616
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 2 cut(s) 116, 384
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 2 cut(s) 116, 384
BmsI GCATC 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 115
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 160, 476
Bse1I ACTGG 1 cut(s) 309
Bse3DI GCAATG 2 cut(s) 137, 396
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 2 cut(s) 205, 379
BseLI CCNNNNNNNGG 3 cut(s) 121, 160, 476
BseMI GCAATG 2 cut(s) 137, 396
BseNI ACTGG 1 cut(s) 309
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 1 cut(s) 548
BshFI GGCC 2 cut(s) 164, 464
BshNI GGYRCC 1 cut(s) 133
BsiHKAI GWGCWC 2 cut(s) 304, 396
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 3 cut(s) 121, 160, 476
BsmAI GTCTC 3 cut(s) 97, 290, 375
BsnI GGCC 2 cut(s) 164, 464
Bsp1286I GDGCHC 2 cut(s) 304, 396
BspACI CCGC 1 cut(s) 18
BspANI GGCC 2 cut(s) 164, 464
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 2 cut(s) 137, 396
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 597
BstBAI YACGTR 1 cut(s) 299
BstF5I GGATG 2 cut(s) 205, 379
BstMAI GTCTC 3 cut(s) 97, 290, 375
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 2 cut(s) 114, 382
BstSLI GKGCMC 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 548
BstXI CCANNNNNNTGG 1 cut(s) 56
BsuRI GGCC 2 cut(s) 164, 464
BtgZI GCGATG 2 cut(s) 339, 447
BtsCI GGATG 2 cut(s) 205, 379
BtsIMutI CAGTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 444
Csp6I GTAC 2 cut(s) 100, 330
CviAII CATG 2 cut(s) 155, 445
CviJI RGCY 3 cut(s) 53, 164, 464
CviKI_1 RGCY 3 cut(s) 53, 164, 464
CviQI GTAC 2 cut(s) 100, 330
Eco147I AGGCCT 2 cut(s) 164, 464
EcoRII CCWGG 1 cut(s) 114
FaeI CATG 2 cut(s) 158, 448
FatI CATG 2 cut(s) 154, 444
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 2 cut(s) 212, 366
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 1 cut(s) 616
GluI GCNGC 1 cut(s) 537
HaeIII GGCC 2 cut(s) 164, 464
HapII CCGG 1 cut(s) 384
Hin1II CATG 2 cut(s) 158, 448
HinfI GANTC 3 cut(s) 227, 283, 421
HpaII CCGG 1 cut(s) 384
Hpy166II GTNNAC 2 cut(s) 296, 302
Hpy188I TCNGA 2 cut(s) 426, 550
Hpy188III TCNNGA 2 cut(s) 287, 445
Hpy8I GTNNAC 2 cut(s) 296, 302
Hpy99I CGWCG 1 cut(s) 590
HpyAV CCTTC 3 cut(s) 253, 284, 363
HpyCH4III ACNGT 1 cut(s) 597
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 3 cut(s) 86, 302, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 2 cut(s) 158, 448
LpnPI CCDG 7 cut(s) 101, 102, 128, 290, 387, 397, 483
Lsp1109I GCAGC 1 cut(s) 548
LweI GCATC 1 cut(s) 424
MaeI CTAG 1 cut(s) 616
MaeII ACGT 1 cut(s) 298
MboII GAAGA 1 cut(s) 460
MhlI GDGCHC 2 cut(s) 304, 396
MluCI AATT 3 cut(s) 145, 451, 515
MlyI GAGTC 2 cut(s) 221, 292
MnlI CCTC 3 cut(s) 257, 385, 454
MseI TTAA 4 cut(s) 108, 243, 255, 599
MslI CAYNNNNRTG 1 cut(s) 443
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 2 cut(s) 116, 384
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 1 cut(s) 384
NlaIII CATG 2 cut(s) 158, 448
NlaIV GGNNCC 1 cut(s) 135
PagI TCATGA 1 cut(s) 444
PceI AGGCCT 2 cut(s) 164, 464
PfeI GAWTC 1 cut(s) 421
PflMI CCANNNNNTGG 1 cut(s) 476
PkrI GCNGC 1 cut(s) 538
PleI GAGTC 2 cut(s) 221, 291
PpsI GAGTC 2 cut(s) 221, 291
Ppu21I YACGTR 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 135
RsaI GTAC 2 cut(s) 101, 331
RsaNI GTAC 2 cut(s) 100, 330
RseI CAYNNNNRTG 1 cut(s) 443
SaqAI TTAA 4 cut(s) 108, 243, 255, 599
SatI GCNGC 1 cut(s) 537
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 2 cut(s) 221, 292
ScrFI CCNGG 2 cut(s) 116, 384
SduI GDGCHC 2 cut(s) 304, 396
SetI ASST 6 cut(s) 55, 91, 249, 295, 301, 581
SfaNI GCATC 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 443
Sse9I AATT 3 cut(s) 145, 451, 515
SseBI AGGCCT 2 cut(s) 164, 464
SsiI CCGC 1 cut(s) 18
SspMI CTAG 1 cut(s) 616
StuI AGGCCT 2 cut(s) 164, 464
StyD4I CCNGG 2 cut(s) 114, 382
TaaI ACNGT 1 cut(s) 597
TaiI ACGT 1 cut(s) 301
TaqI TCGA 3 cut(s) 41, 288, 556
TasI AATT 3 cut(s) 145, 451, 515
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 1 cut(s) 421
Tru1I TTAA 4 cut(s) 108, 243, 255, 599
Tru9I TTAA 4 cut(s) 108, 243, 255, 599
TscAI CASTG 1 cut(s) 309
TseI GCWGC 1 cut(s) 536
TspDTI ATGAA 5 cut(s) 188, 216, 248, 433, 461
TspGWI ACGGA 1 cut(s) 574
TspRI CASTG 1 cut(s) 309
Van91I CCANNNNNTGG 1 cut(s) 476
VneI GTGCAC 1 cut(s) 300
XspI CTAG 1 cut(s) 616
ZrmI AGTACT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.