Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002796

Physical Location & Seq

Genomic Coordinates Reverse (-)

23111875 .. 23113865

Gene Structure 1991 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1141000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1041 bp

ATGGCCACCACAACAAACCTACTGCTTACAGACCTCATGTCTGGTGTGAACCATATCCCTTCAAACTACATCAGACCCATCTCCGATCGTCCAAATTTCACTGATTTTCAGGTATCAGATGCCTCCTCCATTCCTCTCATTGATCTCAAGGACCTCCATGGCCCAACGCACTCGGATATTATCAAACAGATTGGTCTAGCATGCCAGACTGATGGTTTCTTTGAGGTGAAAAATCATGGAGTTCCAGAGATAATGATAAAAGACATGTTGAATATAGCAAGAGAGTTTTTCCAATTACCAGAGAGCGAGAGGTTGAAGATGTACTCAGATGACCCTTCTAAGACAACCAGGCTCTCCACCAGTTTTAACGTCAGGACTGAAAAACTGTCCAATTGGAGGGACTTCCTTAGGCTCCATTGCTACCCTCTTCAAGACTATGTCCAAGAATGGCCTAACAATCCCCCATTGTTCAGGGAGCAAGTGGGTGAGTACTGCACAAATGTGAGAGGATTGGTGCTGAGATTGCTTGAGGCCATATCAGAGAGTTTGGGCTTGGAAAAGAACTGTATTGTTAAGGCATTGGGGAAGCAAGGGCAGCATATGGCTTTGAATTACTATCCACCTTGTCCTCAACCAGAGCTGACTTATGGATTGCCTGGACATACTGATGCCAACCTAATCACCGTCCTTCTCCAAGATGAAGTGCCTGGATTGCAGGTCCTTAGAAATGGCAAGTGGGTTGCTGTCAACCCTATTCCCAACACTTTCATTGTCAATATTGGGGATATGATGCAGGTAATTAGCAACAATCGGTACAAGAGTGTACTCCATAGAGCTGTGGTAAATTGTAACTCAGAGAGAATATCGATCCCAACTTTCTATTGTCCATCACCAGATGCAGTAATTGGGCCTGCCAAGGAGCTCATCAACTATGACCACCCAGCCATGTACAGAAACTTCACATATGCCGAGTACTATGAGAAATTCTGGAATAAAGGACTTGCAACTGAGTGCTGCTTAGACTTGTTTAAACCCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002229 GO:0002239 GO:0006082 GO:0006725 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009696 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010150 GO:0010817 GO:0014070 GO:0016054 GO:0016999 GO:0017001 GO:0017144 GO:0018958 GO:0019336 GO:0019439 GO:0019752 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0042445 GO:0042447 GO:0042493 GO:0042537 GO:0042737 GO:0043207 GO:0043436 GO:0044237 GO:0044248 GO:0044281 GO:0044282 GO:0046244 GO:0046395 GO:0046677 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0071704 GO:0072329 GO:0090693 GO:0098542 GO:0099402 GO:1901360 GO:1901361 GO:1901575 GO:1901615 GO:1901616 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

347

Amino Acids

39.42

Weight (kDa)

5.87

Isoelectric Point (pI)

41.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	44 - 152	1.2e-25	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	199 - 296	8.5e-39	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002796)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10490 AT4G10500
fragaria_vesca	FvH4_5g10430 FvH4_7g17840
malus_domestica	MD01G1090000.v1.1 MD06G1124100.v1.1 MD14G1140400.v1.1 MD14G1140700.v1.1 MD14G1141000.v1.1 MD14G1141200.v1.1
prunus_persica	Prupe.2G199700_v2.0.a1 Prupe.2G200200_v2.0.a1 Prupe.2G200200_v2.0.a1 Prupe.2G200200_v2.0.a1 Prupe.5G134500_v2.0.a1 Prupe.5G134700_v2.0.a1
pyrus_communis	pycom01g11450 pycom06g11450 pycom14g11590
rosa_chinensis	RchiOBHm_Chr1g0361261 RchiOBHm_Chr7g0187801
rosa_laevigata	RLG00000004755 RLG00000027730
rosa_multiflora	Rmu_sc0002119.1_g000038 Rmu_sc0004483.1_g000038
rosa_roxburghii	Rroxscaffold_3G00267060 Rroxscaffold_4G00295120
rosa_rugosa	Rorug01G0287700 Rorug01G0287700 Rorug02G0186700 Rorug06G0484300
rosa_samantha	Rh1AG299000 Rh1BG262900 Rh1CG280300 Rh1DG292900 Rh7CG090300 Rh7DG091900
rosa_wichuraiana	Rw1G026450 Rw7G007720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	706, 784
AclWI	GGATC	1 cut(s)	862
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	94, 983
AfaI	GTAC	6 cut(s)	323, 491, 815, 825, 950, 974
AfiI	CCNNNNNNNGG	1 cut(s)	396
AflIII	ACRYGT	1 cut(s)	264
AgsI	TTSAA	5 cut(s)	63, 271, 316, 431, 610
AhdI	GACNNNNNGTC	1 cut(s)	37
AjnI	CCWGG	3 cut(s)	347, 655, 706
AleI	CACNNNNGTG	1 cut(s)	500
AluBI	AGCT	3 cut(s)	640, 836, 922
AluI	AGCT	3 cut(s)	640, 836, 922
Alw21I	GWGCWC	1 cut(s)	924
AlwI	GGATC	1 cut(s)	862
AoxI	GGCC	5 cut(s)	3, 160, 449, 531, 908
ApeKI	GCWGC	2 cut(s)	595, 1014
ApoI	RAATTY	2 cut(s)	94, 983
AspS9I	GGNCC	4 cut(s)	151, 161, 718, 908
AsuHPI	GGTGA	4 cut(s)	238, 497, 673, 882
AvaII	GGWCC	2 cut(s)	151, 718
AxyI	CCTNAGG	1 cut(s)	407
BaeI	ACNNNNGTAYC	2 cut(s)	805, 838
BalI	TGGCCA	1 cut(s)	5
BanII	GRGCYC	1 cut(s)	924
BarI	GAAGNNNNNNTAC	2 cut(s)	941, 973
Bbv12I	GWGCWC	1 cut(s)	924
BbvI	GCAGC	2 cut(s)	607, 1001
BccI	CCATC	3 cut(s)	86, 206, 895
BciT130I	CCWGG	3 cut(s)	349, 657, 708
BfaI	CTAG	1 cut(s)	197
BfuAI	ACCTGC	2 cut(s)	706, 784
BisI	GCNGC	2 cut(s)	596, 1015
BlsI	GCNGC	2 cut(s)	597, 1016
BmcAI	AGTACT	2 cut(s)	491, 974
Bme1390I	CCNGG	3 cut(s)	349, 657, 708
Bme18I	GGWCC	2 cut(s)	151, 718
BmeRI	GACNNNNNGTC	1 cut(s)	37
BmgT120I	GGNCC	4 cut(s)	151, 161, 718, 908
BmiI	GGNNCC	1 cut(s)	413
BmrFI	CCNGG	3 cut(s)	349, 657, 708
BmsI	GCATC	4 cut(s)	109, 658, 780, 886
BpuEI	CTTGAG	2 cut(s)	131, 548
Bsa29I	ATCGAT	1 cut(s)	866
BsaJI	CCNNGG	2 cut(s)	157, 915
Bsc4I	CCNNNNNNNGG	1 cut(s)	396
Bse1I	ACTGG	1 cut(s)	360
Bse21I	CCTNAGG	1 cut(s)	407
Bse3DI	GCAATG	1 cut(s)	415
BseBI	CCWGG	3 cut(s)	349, 657, 708
BseCI	ATCGAT	1 cut(s)	866
BseDI	CCNNGG	2 cut(s)	157, 915
BseLI	CCNNNNNNNGG	1 cut(s)	396
BseMI	GCAATG	1 cut(s)	415
BseMII	CTCAG	4 cut(s)	339, 509, 867, 999
BseNI	ACTGG	1 cut(s)	360
BseRI	GAGGAG	1 cut(s)	115
BseXI	GCAGC	2 cut(s)	607, 1001
BseYI	CCCAGC	1 cut(s)	940
BsgI	GTGCAG	1 cut(s)	478
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	5 cut(s)	5, 162, 451, 533, 910
BshVI	ATCGAT	1 cut(s)	866
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	1 cut(s)	924
BslFI	GGGAC	1 cut(s)	413
BslI	CCNNNNNNNGG	1 cut(s)	396
BsmFI	GGGAC	1 cut(s)	413
BsnI	GGCC	5 cut(s)	5, 162, 451, 533, 910
Bsp1286I	GDGCHC	1 cut(s)	924
Bsp1407I	TGTACA	1 cut(s)	948
Bsp143I	GATC	3 cut(s)	85, 142, 867
Bsp19I	CCATGG	1 cut(s)	157
BspANI	GGCC	5 cut(s)	5, 162, 451, 533, 910
BspCNI	CTCAG	4 cut(s)	338, 510, 866, 1000
BspDI	ATCGAT	1 cut(s)	866
BspLI	GGNNCC	1 cut(s)	413
BspMI	ACCTGC	2 cut(s)	706, 784
BspPI	GGATC	1 cut(s)	862
BsrDI	GCAATG	1 cut(s)	415
BsrGI	TGTACA	1 cut(s)	948
BsrI	ACTGG	1 cut(s)	360
BssECI	CCNNGG	2 cut(s)	157, 915
BssMI	GATC	3 cut(s)	85, 142, 867
BssT1I	CCWWGG	2 cut(s)	157, 915
Bst2UI	CCWGG	3 cut(s)	349, 657, 708
Bst4CI	ACNGT	3 cut(s)	387, 566, 685
Bst6I	CTCTTC	1 cut(s)	432
BstAUI	TGTACA	1 cut(s)	948
BstC8I	GCNNGC	2 cut(s)	202, 912
BstDEI	CTNAG	8 cut(s)	325, 339, 407, 518, 722, 853, 1008, 1018
BstDSI	CCRYGG	1 cut(s)	157
BstKTI	GATC	3 cut(s)	88, 145, 870
BstMBI	GATC	3 cut(s)	85, 142, 867
BstMCI	CGRYCG	1 cut(s)	88
BstMWI	GCNNNNNNNGC	3 cut(s)	523, 595, 712
BstNI	CCWGG	3 cut(s)	349, 657, 708
BstNSI	RCATGY	2 cut(s)	204, 268
BstSCI	CCNGG	3 cut(s)	347, 655, 706
BstV1I	GCAGC	2 cut(s)	607, 1001
BstXI	CCANNNNNNTGG	1 cut(s)	212
Bsu15I	ATCGAT	1 cut(s)	866
Bsu36I	CCTNAGG	1 cut(s)	407
BsuRI	GGCC	5 cut(s)	5, 162, 451, 533, 910
BsuTUI	ATCGAT	1 cut(s)	866
BtgI	CCRYGG	1 cut(s)	157
BtsIMutI	CAGTG	1 cut(s)	99
BveI	ACCTGC	2 cut(s)	706, 784
Cac8I	GCNNGC	2 cut(s)	202, 912
Cfr13I	GGNCC	4 cut(s)	151, 161, 718, 908
ClaI	ATCGAT	1 cut(s)	866
Csp6I	GTAC	6 cut(s)	322, 490, 814, 824, 949, 973
CviAII	CATG	6 cut(s)	37, 158, 201, 236, 265, 946
CviQI	GTAC	6 cut(s)	322, 490, 814, 824, 949, 973
DdeI	CTNAG	8 cut(s)	325, 339, 407, 518, 722, 853, 1008, 1018
DpnI	GATC	3 cut(s)	87, 144, 869
DpnII	GATC	3 cut(s)	85, 142, 867
DraI	TTTAAA	1 cut(s)	1030
DriI	GACNNNNNGTC	1 cut(s)	37
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	432
Eam1105I	GACNNNNNGTC	1 cut(s)	37
EarI	CTCTTC	1 cut(s)	432
Ecl136II	GAGCTC	1 cut(s)	922
Eco130I	CCWWGG	2 cut(s)	157, 915
Eco24I	GRGCYC	1 cut(s)	924
Eco47I	GGWCC	2 cut(s)	151, 718
Eco53kI	GAGCTC	1 cut(s)	922
Eco81I	CCTNAGG	1 cut(s)	407
EcoICRI	GAGCTC	1 cut(s)	922
EcoO109I	RGGNCCY	2 cut(s)	151, 718
EcoRII	CCWGG	3 cut(s)	347, 655, 706
EcoT14I	CCWWGG	2 cut(s)	157, 915
EcoT38I	GRGCYC	1 cut(s)	924
ErhI	CCWWGG	2 cut(s)	157, 915
FaeI	CATG	6 cut(s)	40, 161, 204, 239, 268, 949
FaqI	GGGAC	1 cut(s)	413
FatI	CATG	6 cut(s)	36, 157, 200, 235, 264, 945
FauNDI	CATATG	2 cut(s)	600, 964
Fnu4HI	GCNGC	2 cut(s)	596, 1015
FriOI	GRGCYC	1 cut(s)	924
Fsp4HI	GCNGC	2 cut(s)	596, 1015
FspBI	CTAG	1 cut(s)	197
GluI	GCNGC	2 cut(s)	596, 1015
GsaI	CCCAGC	1 cut(s)	944
HaeIII	GGCC	5 cut(s)	5, 162, 451, 533, 910
Hin1II	CATG	6 cut(s)	40, 161, 204, 239, 268, 949
HincII	GTYRAC	1 cut(s)	748
HindII	GTYRAC	1 cut(s)	748
HphI	GGTGA	4 cut(s)	238, 497, 673, 882
Hpy166II	GTNNAC	3 cut(s)	49, 748, 824
Hpy188I	TCNGA	7 cut(s)	74, 85, 118, 175, 328, 541, 856
Hpy188III	TCNNGA	4 cut(s)	245, 373, 431, 988
Hpy8I	GTNNAC	3 cut(s)	49, 748, 824
HpyAV	CCTTC	3 cut(s)	69, 345, 698
HpyCH4III	ACNGT	3 cut(s)	387, 566, 685
HpyCH4IV	ACGT	1 cut(s)	369
HpyCH4V	TGCA	5 cut(s)	495, 715, 793, 899, 1004
HpyF10VI	GCNNNNNNNGC	3 cut(s)	523, 595, 712
HpyF3I	CTNAG	8 cut(s)	325, 339, 407, 518, 722, 853, 1008, 1018
HpySE526I	ACGT	1 cut(s)	369
Hsp92II	CATG	6 cut(s)	40, 161, 204, 239, 268, 949
Kzo9I	GATC	3 cut(s)	85, 142, 867
LmnI	GCTCC	3 cut(s)	417, 475, 919
Lsp1109I	GCAGC	2 cut(s)	607, 1001
LweI	GCATC	4 cut(s)	109, 658, 780, 886
MaeI	CTAG	1 cut(s)	197
MaeII	ACGT	1 cut(s)	369
MaeIII	GTNAC	1 cut(s)	848
MalI	GATC	3 cut(s)	87, 144, 869
MboI	GATC	3 cut(s)	85, 142, 867
MboII	GAAGA	2 cut(s)	328, 419
MfeI	CAATTG	1 cut(s)	391
MhlI	GDGCHC	1 cut(s)	924
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	8 cut(s)	94, 293, 391, 610, 798, 844, 903, 983
MluNI	TGGCCA	1 cut(s)	5
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	366, 573, 1029
MslI	CAYNNNNRTG	2 cut(s)	500, 666
Msp20I	TGGCCA	1 cut(s)	5
MspR9I	CCNGG	3 cut(s)	349, 657, 708
MssI	GTTTAAAC	1 cut(s)	1030
MunI	CAATTG	1 cut(s)	391
MvaI	CCWGG	3 cut(s)	349, 657, 708
MwoI	GCNNNNNNNGC	3 cut(s)	523, 595, 712
NcoI	CCATGG	1 cut(s)	157
NdeI	CATATG	2 cut(s)	600, 964
NdeII	GATC	3 cut(s)	85, 142, 867
NlaIII	CATG	6 cut(s)	40, 161, 204, 239, 268, 949
NlaIV	GGNNCC	1 cut(s)	413
NmeAIII	GCCGAG	1 cut(s)	994
NspI	RCATGY	2 cut(s)	204, 268
OliI	CACNNNNGTG	1 cut(s)	500
PaeI	GCATGC	1 cut(s)	204
PciI	ACATGT	1 cut(s)	264
PflFI	GACNNNGTC	1 cut(s)	437
PkrI	GCNGC	2 cut(s)	597, 1016
Ple19I	CGATCG	1 cut(s)	88
PmeI	GTTTAAAC	1 cut(s)	1030
PpuMI	RGGWCCY	2 cut(s)	151, 718
PscI	ACATGT	1 cut(s)	264
Psp124BI	GAGCTC	1 cut(s)	924
Psp5II	RGGWCCY	2 cut(s)	151, 718
Psp6I	CCWGG	3 cut(s)	347, 655, 706
PspFI	CCCAGC	1 cut(s)	940
PspGI	CCWGG	3 cut(s)	347, 655, 706
PspN4I	GGNNCC	1 cut(s)	413
PspPI	GGNCC	4 cut(s)	151, 161, 718, 908
PspPPI	RGGWCCY	2 cut(s)	151, 718
PsyI	GACNNNGTC	1 cut(s)	437
PvuI	CGATCG	1 cut(s)	88
RsaI	GTAC	6 cut(s)	323, 491, 815, 825, 950, 974
RsaNI	GTAC	6 cut(s)	322, 490, 814, 824, 949, 973
RseI	CAYNNNNRTG	2 cut(s)	500, 666
SacI	GAGCTC	1 cut(s)	924
SaqAI	TTAA	3 cut(s)	366, 573, 1029
SatI	GCNGC	2 cut(s)	596, 1015
Sau3AI	GATC	3 cut(s)	85, 142, 867
Sau96I	GGNCC	4 cut(s)	151, 161, 718, 908
ScaI	AGTACT	2 cut(s)	491, 974
ScrFI	CCNGG	3 cut(s)	349, 657, 708
SduI	GDGCHC	1 cut(s)	924
SfaNI	GCATC	4 cut(s)	109, 658, 780, 886
SinI	GGWCC	2 cut(s)	151, 718
SmiMI	CAYNNNNRTG	2 cut(s)	500, 666
SmlI	CTYRAG	2 cut(s)	146, 527
SmoI	CTYRAG	2 cut(s)	146, 527
SphI	GCATGC	1 cut(s)	204
Sse9I	AATT	8 cut(s)	94, 293, 391, 610, 798, 844, 903, 983
SspI	AATATT	1 cut(s)	778
SspMI	CTAG	1 cut(s)	197
SstI	GAGCTC	1 cut(s)	924
StyD4I	CCNGG	3 cut(s)	347, 655, 706
StyI	CCWWGG	2 cut(s)	157, 915
TaaI	ACNGT	3 cut(s)	387, 566, 685
TaiI	ACGT	1 cut(s)	372
TaqI	TCGA	1 cut(s)	866
TasI	AATT	8 cut(s)	94, 293, 391, 610, 798, 844, 903, 983
TatI	WGTACW	5 cut(s)	321, 489, 823, 948, 972
Tru1I	TTAA	3 cut(s)	366, 573, 1029
Tru9I	TTAA	3 cut(s)	366, 573, 1029
TscAI	CASTG	1 cut(s)	106
TseI	GCWGC	2 cut(s)	595, 1014
TspDTI	ATGAA	2 cut(s)	714, 757
TspRI	CASTG	1 cut(s)	106
Tth111I	GACNNNGTC	1 cut(s)	437
VpaK11BI	GGWCC	2 cut(s)	151, 718
XapI	RAATTY	2 cut(s)	94, 983
XceI	RCATGY	2 cut(s)	204, 268
XspI	CTAG	1 cut(s)	197
ZrmI	AGTACT	2 cut(s)	491, 974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.