Rw1G026450

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002796
Physical Location & Seq
Reverse (-)
53585037 .. 53586261
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026450.1

Sequence Viewer

Length: 633 bp
ATGGCACCTCCAAATTCAGCACTCATATCCGACCTTGTATCTGGTGCAACCCATGTTCCTTCTCAATTCGTCCGACCCGAACATGATCGTCCCAATCTTGACCAGGTCATTCAGTCCTCTGATATGTCCATTCCTCTCATCGACCTCCAAGATTTCGACGGCTCACGGCGCTCAGAAATCATTAAGCAGATCGGCCTTGCCTGCCAAGACTATGGCTTTTTTCAGGTTAAAAACCATGGAATTGACGAGGCAGTGATCGATAACTTGTTGGCTGTGGCAAAGGAGTTTTTCCATTTGCCAGAGAGTGAAAGGATGAAGGTTTACTCTGAAGACCCTTATAAGACTATAAGACTGTCGACTAGCTTCAATGTCAGAGTTGAGGAAGTATCCTGCTGGAGAGATTACCTAAGACTCCACTGCCAACCTCTGGAGGATTACATTCATGAGTGGCCAGCCAACCCTCCCTCTTTCAGGGAAGATGTGGCAGAGTATTGCAAGAAAGTCAGAGGCCTGGCAGTGAGACTCCTTCAAGGCATATCTGAAAGCCTGGGACTGGAACGAGACTACGGCTGTGTTTGGGGGGGCTTTTTTGCTCCCCCGCTTATAAGCAGAAGACCCATAAGTGTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

210

Amino Acids

23.99

Weight (kDa)

5.25

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 44 - 152 2.4e-30 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 339, 605
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 427
AccI GTMKAC 1 cut(s) 356
AciI CCGC 1 cut(s) 599
AcoI YGGCCR 1 cut(s) 449
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 348
AfiI CCNNNNNNNGG 3 cut(s) 427, 471, 553
AgsI TTSAA 2 cut(s) 367, 530
AjnI CCWGG 3 cut(s) 102, 510, 546
AluBI AGCT 1 cut(s) 363
AluI AGCT 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 514, 555
AoxI GGCC 3 cut(s) 193, 449, 508
ApoI RAATTY 1 cut(s) 13
AspLEI GCGC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 451
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 336, 619
BceAI ACGGC 3 cut(s) 175, 182, 583
BciT130I CCWGG 3 cut(s) 104, 512, 548
BciVI GTATCC 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 514, 555
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 172
BfuI GTATCC 1 cut(s) 397
Bme1390I CCNGG 3 cut(s) 104, 512, 548
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 104, 512, 548
BpiI GAAGAC 2 cut(s) 336, 619
BpmI CTGGAG 2 cut(s) 415, 449
Bsa29I ATCGAT 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 235, 547
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 471, 553
Bse1I ACTGG 1 cut(s) 558
BseBI CCWGG 3 cut(s) 104, 512, 548
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 235, 547
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 427, 471, 553
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 558
BshFI GGCC 3 cut(s) 195, 451, 510
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 258
BslFI GGGAC 2 cut(s) 75, 564
BslI CCNNNNNNNGG 3 cut(s) 427, 471, 553
BsmAI GTCTC 2 cut(s) 514, 555
BsmFI GGGAC 2 cut(s) 75, 564
BsnI GGCC 3 cut(s) 195, 451, 510
Bsp143I GATC 3 cut(s) 85, 189, 255
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 1 cut(s) 599
BspANI GGCC 3 cut(s) 195, 451, 510
BspCNI CTCAG 1 cut(s) 185
BspDI ATCGAT 1 cut(s) 258
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 558
BssECI CCNNGG 2 cut(s) 235, 547
BssMI GATC 3 cut(s) 85, 189, 255
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 3 cut(s) 104, 512, 548
Bst4CI ACNGT 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 202, 453
BstDEI CTNAG 2 cut(s) 172, 407
BstDSI CCRYGG 1 cut(s) 235
BstENI CCTNNNNNAGG 1 cut(s) 469
BstF5I GGATG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 3 cut(s) 88, 192, 258
BstMAI GTCTC 2 cut(s) 514, 555
BstMBI GATC 3 cut(s) 85, 189, 255
BstMWI GCNNNNNNNGC 2 cut(s) 168, 201
BstNI CCWGG 3 cut(s) 104, 512, 548
BstSCI CCNGG 3 cut(s) 102, 510, 546
BstV2I GAAGAC 2 cut(s) 336, 619
BstXI CCANNNNNNTGG 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 258
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 3 cut(s) 195, 451, 510
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 3 cut(s) 258, 415, 522
BtsIMutI CAGTG 3 cut(s) 258, 415, 522
Cac8I GCNNGC 2 cut(s) 202, 453
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 171
ClaI ATCGAT 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 102
CviAII CATG 4 cut(s) 53, 83, 236, 443
DdeI CTNAG 2 cut(s) 172, 407
DpnI GATC 3 cut(s) 87, 191, 257
DpnII GATC 3 cut(s) 85, 189, 255
EaeI YGGCCR 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 348
EcoNI CCTNNNNNAGG 1 cut(s) 469
EcoRII CCWGG 3 cut(s) 102, 510, 546
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 4 cut(s) 56, 86, 239, 446
FaqI GGGAC 2 cut(s) 75, 564
FatI CATG 4 cut(s) 52, 82, 235, 442
FauI CCCGC 1 cut(s) 606
FblI GTMKAC 1 cut(s) 356
FokI GGATG 1 cut(s) 325
FspBI CTAG 1 cut(s) 360
GlaI GCGC 1 cut(s) 170
GsuI CTGGAG 2 cut(s) 415, 449
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 195, 451, 510
HhaI GCGC 1 cut(s) 171
Hin1II CATG 4 cut(s) 56, 86, 239, 446
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 2 cut(s) 411, 522
Hpy166II GTNNAC 2 cut(s) 322, 357
Hpy188I TCNGA 8 cut(s) 31, 74, 121, 175, 328, 374, 506, 541
Hpy188III TCNNGA 3 cut(s) 98, 428, 443
Hpy8I GTNNAC 2 cut(s) 322, 357
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 69, 310, 536
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 47, 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 201
HpyF3I CTNAG 2 cut(s) 172, 407
Hsp92II CATG 4 cut(s) 56, 86, 239, 446
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 85, 189, 255
LmnI GCTCC 1 cut(s) 598
MabI ACCWGGT 1 cut(s) 102
MaeI CTAG 1 cut(s) 360
MalI GATC 3 cut(s) 87, 191, 257
MboI GATC 3 cut(s) 85, 189, 255
MboII GAAGA 3 cut(s) 341, 488, 624
MlsI TGGCCA 1 cut(s) 451
MluCI AATT 3 cut(s) 13, 65, 240
MluNI TGGCCA 1 cut(s) 451
MlyI GAGTC 2 cut(s) 405, 516
MmeI TCCRAC 2 cut(s) 54, 97
Mox20I TGGCCA 1 cut(s) 451
MscI TGGCCA 1 cut(s) 451
MseI TTAA 2 cut(s) 183, 228
Msp20I TGGCCA 1 cut(s) 451
MspR9I CCNGG 3 cut(s) 104, 512, 548
MvaI CCWGG 3 cut(s) 104, 512, 548
MwoI GCNNNNNNNGC 2 cut(s) 168, 201
NcoI CCATGG 1 cut(s) 235
NdeII GATC 3 cut(s) 85, 189, 255
NlaIII CATG 4 cut(s) 56, 86, 239, 446
NlaIV GGNNCC 1 cut(s) 6
PagI TCATGA 1 cut(s) 442
PceI AGGCCT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PflFI GACNNNGTC 1 cut(s) 104
PflMI CCANNNNNTGG 1 cut(s) 427
PleI GAGTC 2 cut(s) 405, 516
PpsI GAGTC 2 cut(s) 405, 516
PsiI TTATAA 2 cut(s) 339, 605
Psp6I CCWGG 3 cut(s) 102, 510, 546
PspGI CCWGG 3 cut(s) 102, 510, 546
PspN4I GGNNCC 1 cut(s) 6
PsyI GACNNNGTC 1 cut(s) 104
SalI GTCGAC 1 cut(s) 355
SaqAI TTAA 2 cut(s) 183, 228
Sau3AI GATC 3 cut(s) 85, 189, 255
SchI GAGTC 2 cut(s) 405, 516
ScrFI CCNGG 3 cut(s) 104, 512, 548
SetI ASST 9 cut(s) 10, 36, 108, 147, 228, 321, 365, 408, 427
SexAI ACCWGGT 1 cut(s) 102
Sse9I AATT 3 cut(s) 13, 65, 240
SseBI AGGCCT 1 cut(s) 510
SsiI CCGC 1 cut(s) 599
SspMI CTAG 1 cut(s) 360
StuI AGGCCT 1 cut(s) 510
StyD4I CCNGG 3 cut(s) 102, 510, 546
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 354
TaqI TCGA 4 cut(s) 141, 156, 258, 356
TasI AATT 3 cut(s) 13, 65, 240
Tru1I TTAA 2 cut(s) 183, 228
Tru9I TTAA 2 cut(s) 183, 228
TscAI CASTG 3 cut(s) 258, 422, 522
TspDTI ATGAA 2 cut(s) 329, 431
TspRI CASTG 3 cut(s) 258, 422, 522
Tth111I GACNNNGTC 1 cut(s) 104
Van91I CCANNNNNTGG 1 cut(s) 427
XagI CCTNNNNNAGG 1 cut(s) 469
XapI RAATTY 1 cut(s) 13
XmiI GTMKAC 1 cut(s) 356
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.